检索结果-南通市图书馆

Molecular Studies on Heat Shock Protein 70 Gene （Hsp70） Using ClustalW Multiple Sequence Alignment Analy.is

Journal of Life Sciences 2010年第6期4卷 1-8页

作者： Mohamed A. Ezz Mohamed H. Soliman abd alkader y. gamal.el-din Aly.Z.E. abdelsalam Molecular Genetics Lab Biotechnology College Misr University for Science and Technology 6th of October City October 12451 Egypt Department of Genetics Faculty of Agriculture Cairo University Giza 12613 Egypt A in Shams Center for Genetics Engineering and Biotechnology Faculty of Agriculture Ain Shams University Cairo 11241 Egypt

Degenerate primers are particularly.useful in amplify.ng homologous genes from different organisms. This paper describes a method for designing degenerate primers for a given multiple alignment of DNA sequences of Hsp70 gene family.using ClustalW algorithm. The authors used an in silico approach to find a homology.between more than one accession numbers of DNA sequences, X67711.2 was for Ory.a sativa Hsp70, Ay.72071.1 was for Nicotiana tabacum Hsp70 and L41253.2 was for Ly.opersicon esculentum Hsc70. The three accession numbers which were retrieved by.the BLASTn program depend on their expected value （E-value）. Multiple sequence alignment was performed by.ClustalW algorithm to produce a conserved blocks and determine the consensus region which had been used to produce the forward and reverse primer by.the primer select module of DNAStar Lasergene V7 and In-Silco PCR module of FASTPCR program ver.4.0.8 was performed to detect the melting temperatures （Tm） and predict the PCR product size, The results of designed degenerate primer showed that there was a homology.found between the designed primers and the DNA templates for the three accession numbers with at least 80% identity. The result of degenerate PCR showed that the three bands of the amplified PCR products of the three accession numbers were detected at the same molecular weight of marker （400 bp） with a difference about 15 pb compared to the in silco PCR product （385 pb）. In conclusion, this study.focused on the importance of using the clustalW algorithm for designing the degenerate primer.

关键词： ClustalW tool degenerate PCR different melting temperatures In-Silico PCR.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：