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The potential utility of nested PCR for investigation of Coxiella burnetii in Iranian snails

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Journal of Coastal Life Medicine 2016年第3期4卷 193-196页

作者： Mina Dehghani-Samani abbas doosti Asghar Arshi Young Researchers and Elite Club Shahrekord BranchIslamic Azad UniversityShahrekordIran Biotechnology Research Center Islamic Azad UniversityShahrekord BranchShahrekordIran

Objective:To detect the prevalence of Coxiella burnetii(***)in two species of snails consisted of Lymnaea palustris(***)and Pomacea canaliculata(***)by using nested PCR method in Chaharmahel Va Bakhtiari Province which is located in the southwest of ***:A total of 160 snail samples consisted of 100 *** and 60 *** were collected from 4 rice paddy fields in the southwest of Iran between June and August ***'DNA was extracted by a genomic DNA purification kit according to the manufacturer's *** of the presence of ***'s DNA was carried out by using a nested PCR assay with[specific primers outer membrane protein 1(OMP1)-OMP2 and OMP3-OMP4]targeting the com1 ***:In this study,a total of 160 snail samples were tested and 15(9.37%)samples were found positive for ***,15 samples were positive from the *** and there were no positive samples from ***:Snails are kind of gastropods which seem to be harmless in life,but these small gastropods can be very dangerous for farmers,especially in humid ***,*** in snails showed that this bacterium can be a factor of transmission of contamination to human beings and animals.

关键词： Coxiella burnetii Nested PCR Snails Iran

The relationship between <i>Helicobacter pylori</i>disease and bacterial count in stomach

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Health 2014年第4期6卷 259-262页

作者： Sadegh Ghorbani-Dalini Mohammad Kargar abbas doosti Akram Najafi Biotechnology Research Center Shahrekord Branch Islamic Azad University Shahrekord Iran Department of Biology University of Sistan and Baluchestan Zahedan Iran Department of Marine Microbiology The Persian Gulf Marine Biotechnology Research Center Bushehr University of Medical Science Iran Department of Microbiology Jahrom Branch Islamic Azad University Jahrom Iran

Several studies showed significant relationships between bacterial counts and the severity and type of disease in patients. The aim of this study was to evaluate the relationship between Helicobacter pylori disease and bacterial count. In this study, 287 patients with dyspeptic symptoms were evaluated. Three variables including patient-reported data, clinical signs and bacterial load of gastric specimens were analyzed. Biopsy samples were collected from patients who were referred for endoscopies because of dyspeptic symptoms. Initially, the clinical status was evaluated and recorded by a questionnaire. DNA extraction was performed and H. pylori was analyzed for bacterial count by Real-time PCR assay and specific primers and probe. The variety range of bacteria in specimens was 104 to 1012. The results revealed that a greater relationships existed between 1012 and gastric cancer (p = 0.036), also 104 and acid reflux (p = 0.006) and vomiting (p = 0.047). Real-time PCR assay provides a highly accurate, rapid and precise method for detection of H. pylori and determination of progressive disease due to this bacterium.

关键词： Helicobacter pylori Gastric Disorder Quantitative Real-Time PCR Stomach

Expanding role of antibodies in kidney transplantation

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World Journal of Transplantation 2025年第1期15卷 57-71页

作者： Khawar abbas Muhammed Mubarak Department of Transplant Immunology Sindh Institute of Urology&TransplantationKarachi 74200SindhPakistan Javed I.Kazi Department of Histopathology Sindh Institute of Urology&TransplantationKarachi 74200SindhPakistan

The role of antibodies in kidney transplant(KT)has evolved significantly over the past few *** role of antibodies in KT is multifaceted,encompassing both the challenges they pose in terms of antibody-mediated rejection(AMR)and the opportunities for improving transplant outcomes through better detection,prevention,and treatment *** our understanding of the immunological mechanisms continues to evolve,so too will the approaches to managing and harnessing the power of antibodies in KT,ultimately leading to improved patient and graft *** narrative review explores the multifaceted roles of antibodies in KT,including their involvement in rejection mechanisms,advancements in desensitization protocols,AMR treatments,and their potential role in monitoring and improving graft survival.

关键词： Antibodies Humoral theory Kidney transplantation Rejection

Transport mechanism in chemically reactive hybrid nanofluid flow containing gyrotactic micro-organisms over a curved oscillatory surface

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 177-192页

作者： M.NAVEED M.IMRAN T.ASGHAR Z.abbas Department of Mathematics Division of Science and TechnologyUniversity of Education Department of Mathematics The Islamia University of Bahawalpur

This paper examines the transport analysis, including both heat transfer and mass transfer, in hybrid nanofluid flow containing gyrotactic microorganisms towards a curved oscillatory surface. The influence of magnetic fields is also inspected in terms of their physical characteristics. To depict the phenomena of transport, modified versions of both Fick's and Fourier's laws are used. Additionally, the characteristics of both heterogeneous and homogeneous chemical reactions are also incorporated. Utilizing a curvilinear coordinate system, the flow problem is formulated as partial differential equations(PDEs)for momentum, concentration, microorganism field, and energy. An analytical solution to the obtained flow equations is achieved utilizing the homotopy analysis method(HAM).The effects of significant flow parameters on the pressure and microorganism fields, velocity, oscillation velocity, concentration, and temperature distributions are shown via graphs. Furthermore, the variations in skin friction, mass transfer rate, heat transfer rate, and local motile number due to different involved parameters are presented in tables and are analyzed in detail. Graphical results indicate that the curves of velocity and temperature fields are enhanced as the values of the solid volume fraction variables *** is also verified that the concentration rate field decreases as the values of the homogeneous reaction strength parameter and the radius of curvature parameter increase, and it increases with the Schmidt number and the heterogeneous reaction strength *** outcomes show a favorable response of the motile number to advanced values of the Peclet number, the Schmidt number, the microorganism difference parameter, and the bio-convective Lewis number.

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Introduction to the Special Issue on New Trends in Statistical Computing and Data Science

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Computer Modeling in Engineering & Sciences 2023年第8期136卷 981-983页

作者： Christophe Chesneau Hassan doosti Department of Mathematics LMNOUniversity of CaenCaen14032France School of Mathematical and Physical Sciences Macquarie UniversitySydney2109Australia

The interface between computer science and statistics has developed considerably in recent years,with exponential progress in the fields of data analysis,stochastic modeling,machine learning,econometrics,simulation,algorithms,classification,and *** discoveries in this field appear every day,opening new scientific horizons for the modern *** is especially true in the post-2020 period,with the treatment of large volumes of data that feed the daily operations of large corporations,as well as the development of artificial intelligence,including advanced machine learning techniques,particularly“deep learning”.

关键词： has operations corporation

A Two-Step Algorithm to Estimate Variable Importance for Multi-State Data:An Application to COVID-19

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Computer Modeling in Engineering & Sciences 2023年第6期135卷 2047-2064页

作者： Behnaz Alafchi Leili Tapak Hassan doosti Christophe Chesneau Ghodratollah Roshanaei Department of Biostatistics School of Public HealthModeling of Noncommunicable Diseases Research CenterHamadan University of Medical SciencesHamadanIran School of Mathematical and Physical Sciences Macquarie UniversitySydneyAustralia Department of Mathematics LMNOUniversity of Caen-NormandieCaenFrance

Survival data with amulti-state structure are frequently observed in follow-up *** analytic approach based on a multi-state model(MSM)should be used in longitudinal health studies in which a patient experiences a sequence of clinical progression *** main objective in the MSM framework is variable selection,where attempts are made to identify the risk factors associated with the transition hazard rates or probabilities of disease *** usual variable selection methods,including stepwise and penalized methods,do not provide information about the importance of *** this context,we present a two-step algorithm to evaluate the importance of variables formulti-state *** differentmachine learning approaches(randomforest,gradient boosting,and neural network)as themost widely usedmethods are considered to estimate the variable importance in order to identify the factors affecting disease progression and rank these factors according to their *** performance of our proposed methods is validated by simulation and applied to the COVID-19 data *** results revealed that the proposed two-stage method has promising performance for estimating variable importance.

关键词： Multi-state data deviance residual martingale residual gradient boosting randomforest neural network variable importance variable selection

Applying the spectral stochastic finite element method in multiple-random field RC structures

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Frontiers of Structural and Civil Engineering 2022年第4期16卷 434-447页

作者： abbas YAZDANI Civil Engineering Department University of Sistan and BaluchestanZahedan 98167-45845Iran

This paper uses the spectral stochastic finite element method(SSFEM)for analyzing reinforced concrete(RC)beam/slab *** doing so,it presents a new framework to study how the correlation length of a random field(RF)with uncertain parameters will affect modeling uncertainties and reliability *** considers:1)different correlation lengths for uncertainty parameters,and 2)dead and live loads as well as the elasticity moduli of concrete and steel as a multi-dimensional RF in concrete *** show the SSFEM’s efficiency in the study of concrete structures and to evaluate the sensitivity of the correlation length effects in evaluating the reliability,two examples of RC beams and slabs have been *** to the results,the RF correlation length is effective in modeling uncertainties and evaluating reliabilities;the longer the correlation length,the greater the dispersion range of the structure response and the higher the failure probability.

关键词： uncertainty spectral stochastic finite element method correlation length reliability assessment reinforced concrete beam/slab

Characteristics of settling of dilute suspension of particles with different density at high Reynolds numbers

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Particuology 2021年第3期19卷 62-74页

作者： Ali abbas Zaidi Department of Mechanical and Process Engineering ETH Zurich8092 ZurichSwitzerland

Dilute suspension of particles with same density and size develops clusters when settle at high Reynolds number(≥250).It is due to particles entrapment in the wakes produced by upstream *** this work,this phenomenon is studied for suspension having particles with different densities by numerical *** particle-fluid interactions are modelled using immersed boundary method and inter-particle collisions are modelled using discrete element *** simulations,settling Reynolds number is always kept above 250 and the suspension solid volume fraction is nearly 0.1 *** particle density ratios(*** of heavy particles to lighter particles)equal to 4:1 and 2:1 and particles with same density are *** each density ratio,the percentage volume fraction of each particle density is nearly varied from 0.8 to *** characteristics such as microstructures of settling particle,average settling velocity and velocity fluctuations of settling particles are *** show that for different density particles settling characteristics of suspension is largely dominated by heavy *** the end of paper,the underlying physics is explained for the anomalies observed in simulation.

关键词： Particle clustering at high reynolds number Suspension with different density particles Particle microstructure due to settling Immersed boundary method

Photocatalytic degradation evaluation of N–Fe codoped aligned TiO2 nanorods based on the effect of annealing temperature

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Journal of Advanced Ceramics 2020年第1期9卷 107-122页

作者： abbas Sadeghzadeh-Attar Department of Metallurgy and Materials Engineering University of KashanP.O.Box.87317-53153Ghotb Ravandi Blvd.KashanIran

In this paper,a comparative study on the photocatalytic degradation of the Rhodamine B(Rh B)dye as a model compound using N–Fe codoped Ti O2 nanorods under UV and visible-light(λ≥420 nm)irradiations has been *** O2 photocatalysts were fabricated as aligned nanorod arrays by liquid-phase deposition process,annealed at different temperatures from 400 to 800℃.The effects of annealing temperature on the phase structure,crystallinity,BET surface area,and resulting photocatalytic activity of N–Fe codoped Ti O2 nanorods were also *** degradation studies confirmed that the nanorods annealed at 600℃composed of both anatase(79%)and rutile phases(21%)and offered the highest activity and stability among the series of nanorods,as it degraded 94.8%and 87.2%Rh B in 120 min irradiation under UV and visible-light,*** 600℃,the photocatalytic performance of nanorods decreased owning to a phase change,decreased surface area and bandgap,and growth of Ti O2 crystallites induced by the annealing *** is hoped that this work could provide precious information on the design of 1 D catalyst materials with more superior photodegradation properties especially under visible-light for the further industrial applications.

关键词： N–Fe codoped TiO2 nanorods liquid-phase deposition annealing temperature photocatalytic degradation