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Assessment of genotoxicity and cytotoxicity of standardized aqueous extract from leaves of Erythroxylum cuneatum in human HepG2 and WRL68 cells line

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Asian Pacific Journal of Tropical Medicine 2013年第10期6卷 811-816页

作者： RK Wesam an ghanya HH Mizaton M ILham A Aishah Faculty of Pharmacy Universiti Teknologi MARAShah AlamSelangor Department of Food Sciences and Technology Faculty of AgricultureUniversity of Sana'a Forest Research Institute of Malaysia(FRIM)

Objective:To investigate the cytotoxicity and the genotoxicity of standardized aqueous of dry leaves of Erythroxylum cuneatum(***)in human HepG2 and WRL68 ***:The cytoloxicity of *** extract was evaluated by both MTS and LDH *** study on *** extract was assessed by the single cell gel electrophoresis(comet assay).The protective effect of *** against menadione-induced cytotoxicity was also ***:Results from this study showed that *** extract exhibited cytotoxic activities towards the cells with IC_(50)value of(125±12)and(125±14)μg/mL for HepG2and WRL68 cells respectively,after 72 h incubation period as determined by MTS *** leakage was detected at(251±19)and(199.5±12.0)μg/mL for HepG2 and WRL68 *** study results showed that treatment with *** up to 1 mg/mL did not cause obvious DNA damage in WRL68 and HepG2 *** of *** did not show significant protection towards menadione in WRL68 and HepG2 ***:*** standardized aqueous extract might be developed in onler to establish new pharmacological possibilities for its application.

关键词： Erythroxylum cuneatu Cytotoxicity Genotoxicity DNA damage HepG2 WRL68

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Hypoglycemic effect of Octomeles sumatrana aqueous extract in streptozotocin—induced diabetic rats and its molecular mechanisms

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Asian Pacific Journal of Tropical Medicine 2012年第11期5卷 875-881页

作者： Mohd Aidil Azahar ghanya Al-Naqeb Mizaton Hasan Aishah Adam Faculty of Pharmacy Universiti Teknologi MARAPuncak Alam campusBandar Puncak Alam42300SelangorMalaysia

Objective:To investigate the hypoglycemic effect of the aqueous extract of Octomeles sumatrana (***)(OS) in streptozotocin-induced diabetic rats(STZ) and its molecular mechanisms. Methods:Diabetes was induced by intraperitoneal(i.p.) injection of streptozotocin(55 mg/kg) in to male Sprague-Dawley *** were divided into six different groups;normal control rats were not induced with STZ and served as reference,STZ diabetic control rats were given normal *** groups were treated with OS aqueous extract at 0.2,0.3 and 0.5 g/kg,orally twice daily continuously for 21 *** fifth group was treated with glibenclamide(6 mg/kg) in aqueous solution orally continuously for 21 *** completion of the treatment period,biochemical parameters and expression levels of glucose transporter 2(Slc2a2),glucose-6-phosphatase (GoPase) and phosphoenolpyruvate carboxykinase(PCK1) were determined in liver by quantitative real time ***:Administration of OS at different doses to STZ induced diabetic rats, resulted in significant decrease(P<0.05) in blood glucose level in a dose dependent manner by 36%,48%,and 64%at doses of 0.2,0.3 and 0.5 g/kg,respectively,in comparison to the STZ control *** with OS elicited an increase in the expression level of Slc2a2 gene but reduced the expression of G6Pase and PCK1 ***,OS treated rats,showed significantly lower levels of serum alanine transaminase(ALT),aspartate aminotransferase(AST) and urea levels compared to STZ untreated *** extract at different doses elicited signs of recovery in body weight gain when compared to STZ diabetic controls although food and water consumption were significantly lower in treated groups compared to STZ diabetic control ***:*** aqueous extract is beneficial for improvement of hyperglycemia by increasing gene expression of liver Slc2a2 and reducing expression of G6Pase and PCK1 genes in streptozotocin-induced diabetic rats.

关键词： Octomeles sumatrana Aqueous extract Antioxidant Oxidative stress STZ-induced diabetic rats Gene expression

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