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Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridi-zation and cDNA library array

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Science China(Life Sciences) 2004年第2期47卷 148-157页

作者： ai junkui1, ZHANG Zhiwen2, XIN Dianqi1, ZHU Hongjian1, YAN Quanjian3, XIN Zhongcheng1, NA Yanqun1 & GUO Yinglu1 1. Institute of Urology, Peking University & Department of Urology, First Hospital, Peking University, Beijing 100034, China 2. Department of Physiology, Peking University Health Science Center, Beijing 100083, China 3. Department of Urology, Xijing Hospital, the 4th Military Medical University, Xi抋n 710033, China Institute of Urology Peking University & Department of Urology First Hospital Peking University Beijing China Department of Physiology Peking University Health Science Center Beijing China Department of Urology Xijing Hospital The 4th Military Medical University Xi’an China

To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon mem-brane and the over-expressed genes were then screened by hybridizing the filter with radioac-tively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank da-tabase. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular en-dothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohis-tochemical and Western blot analysis for PTTG1 and ZNRD1 revealed increased protein level in RCC. The over-expressed genes in RCC are the potential molecular targets for diagnosis and therapy and it is very important to understand the molecular mechanism of RCC through the profile of over-expressed genes.

关键词： suppression subtractive hybridization (SSH), cDNA microarray, renal cell carcinoma (RCC), gene, differential expression.

Retrieval of aerosol optical constants from angular light-scattering measurement signals using principal component analysis approach

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Particuology 2019年第2期17卷 210-218页

作者： Zhenzong He junkui Mao Xingsi Han Hao Wang Jiangsu Province Key Laboratory of Aerospace Power System College of Energy and Power Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 China

An angular light-scattering measurement (ALSM) method combined with the probability density function-bmsed ant colony optimization algorithm (PDF-ACO) is proposed for retrieval of aerosol optical constants. An optimal measurement angle selection method using a principal component analysis (PCA) approach is developed to improve retrieval accuracy. Results indicate that optimized angle selection can ensure retrieval accuracy. The aerosol optical constants over Beijing, China, which are available from the Aerosol Robotic Network (AERONET), are then rec on structed. The ALSM method's con vergence properties are also studied via comparison w让h those of the light reflection-transmittance measurement (LRTM) method. Results retrieved using the ALSM method show better convergence speed and accuracy than those retrieved using the LRTM method because the ALSM method does not require solution of the radiative transfer equation and allows more useful signals to be obtained. Additionally, the inverse accuracy of the refractive index results is better than that of the absorption index results;this is attributed to differences between the monodromic characteristics of the refractive index and absorption index retrieval results. All results confirm that the combination of the ALSM method w让h the PDF-ACO algorithm and the optimal measurement angle selection method provides effective and reliable aerosol optical constant reconstructio n.

关键词： Angular light-scattering measurement method Aerosol optical constant Ant colony optimization algorithm Principal comp orient analysis Inverse radiation problem

An optimized time-adaptive aerothermal coupling calculation method for aerothermal analysis of disc cavity system

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维普期刊数据库

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Chinese Journal of Aeronautics 2024年第9期37卷 164-177页

作者： Shuai BI junkui MAO Lei WANG Feng HAN College of Energy and Power Engineering Nanjing University of Aeronautics and AstronauticsNanjing 20016China AECC Aeroengine Control System Institute Wuxi 214063China Integrated Energy Institute Nanjing University of Aeronautics and AstronauticsNanjing 210016China

In order to further achieve the balance between the calculation accuracy and efficiency of the transient analysis of the aero-engine disc cavity system,an Optimized Time-adaptive Aerother-mal Coupling calculation(OTAC)method has been *** combines one-dimensional tran-sient calculation of air system,Conventional Sequence Staggered(CSS)method,Time-adaptive Aerothermal Coupling calculation(TAC)method and differential evolution optimization algorithm to obtain an efficient and high-precision aerothermal coupling calculation method of air *** both the heat conduction in the solid domain and the flow in the fluid domain as unsteady states in the OTAC,the interaction of fluid-solid information within a single coupling time step size was implemented based on the CSS ***,the coupling time step size was automatically adjusted with the number of iterations by using the Proportional-Integral-Deri vative(PID)*** show that when compared with the traditional loosely coupling method with a fixed time step size,the computational accuracy and efficiency of the OTAC method are improved by 8.9%and 30%,*** with the tight coupling calculation,the OTAC method can achieve a speedup of 1 to 2 orders of magnitude,while the calculation error is maintained within 6.1%.

关键词： air system 1D-3D coupling Conjugate heat transfer Transient calculation Adaptive time step size

Effective Thermal Conductivity for 3D Five-Directional Braided Composites Based on Microstructural Analysis

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Transactions of Nanjing University of Aeronautics and Astronautics 2019年第1期36卷 128-138页

作者： ZHAO Xiao MAO junkui JIANG Hua Key Laboratory of Aerospace Power System College of Energy and Power EngineeringNanjing University of Aeronautics and Astronautics Cummins(China) Investment Co. Ltd. Commercial aircraft Corporation of China Ltd.

A method for predicting effective thermal conductivities(ETCs) of three-dimensional five-directional(3D5D) braided composites is presented. The effective thermal conductivity prediction method contains a digital image processing technology. Multiple scanning electron microscopy(SEM)images of composites are analyzed to obtain actual microstructural features. These actual microstructural features of 3D5D braided composites are introduced into representative volume element(RVE) modeling. Apart from applying actual microstructural features,compression effects between yarns are considered in the modeling of RVE,making the RVE more realistic. Therefore,the ETC prediction method establishes a representative unit cell model that better reflects the true microstructural characteristics of the 3D5D braided composites. The ETCs are predicted with the finite element method. Then thermal conductivity measurements are carried out for a 3D5D braided composite *** comparing the predicted ETC with the measured thermal conductivity, the whole process of the ETC prediction method is proved to be effective and accurate,where a relative error of only 2.9 % is ***,the effects of microstructural features are investigated,indicating that increasing interior braiding angles and fiber fill factor can lead to higher transverse ETCs. Longitudinal ETCs decrease with increasing interior braiding angles,but increase with increasing fiber fill factor. Finally,the influence of variations of microstructure parameters observed in digital image processing are investigated. To explore the influence of variations in microstructural features on variations in predicted ETCs,the actual probability distributions of microstructural features obtained from the 3D5D braided composite sample are introduced into the ETC investigation. The results show that,compared with the interior braiding angle,variations in the fiber fill factor exhibit more significant effects on variations in ETCs.

关键词： effective thermal conductivity digital image processing variation 3D five-directional braided composites

Unsteady analysis of jet impingement under vibration conditions

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Chinese Journal of Aeronautics 2022年第5期35卷 291-308页

作者： Yue YANG junkui MAO Feilong WANG Xingsi HAN Jiangsu Province Key Laboratory of Aerospace Power System Nanjing University of Aeronautics and AstronauticsNanjing 210016China

The vibration of thermodynamic machinery will affect its cooling *** this research,a high-resolution simulation of jet impingement was performed to quantify the unsteady turbulent convection under vibration conditions.A newly developed Self-Adaptive Turbulence Eddy Simulation(SATES)method was *** Reynolds number was Re=23000,the jet-towall distance was fixed at H/D=2,and the vibrating frequency of the impinging wall f varied from 0 to 200 *** with the static wall case,the maximum enhancement of the stagnation point and area averaged Nusselt number within r/D=1 could reach up to 5%due to the larger primary vortices,whereas it could reduce the heat transfer by 10%beyond r/D=3 due to the suppression of the wall vortices *** on the unsteady analysis and Proper Orthogonal Decomposition(POD)pattern,the modes controlled by vibration were recognized and their contributions to the heat transfer performance were also *** introduction of the vibration promoted the development of the primary vortices and changed the radial alternating motion to a vertical alternating motion at the wall jet *** former was beneficial for the heat transfer,while the latter was unfavorable.

关键词： Impinging jet Proper orthogonal decomposition Self-Adaptive Turbulence Eddy Simulation(SATES) Unsteady turbulent convection Vibration

Disinfection byproduct formation from algal organic matters after ozonation or ozone combined with activated carbon treatment with subsequent chlorination

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Journal of Environmental Sciences 2021年第6期33卷 233-241页

作者： Qiufeng Lin Feilong Dong Cong Li junkui Cui School of Environment and Architecture University of Shanghai for Science and TechnologyShanghai200433China Department of Earth and Environmental Studies Montclair State UniversityMontclairNew Jersey 07043United States College of Environment Zhejiang University of TechnologyHangzhou310032China

Algal organic matter(AOM),including extracellular organic matter(EOM)and intracellular organic matter(IOM)from algal blooms,is widely accepted as essential precursors of disinfection byproducts(DBPs).This study evaluated the effect of ozonation or ozone combined with activated carbon(O_(3)-AC)treatment on characteristic alternation and DBP formation with subsequent chlorination of Chlorella sp..The effects of p H and bromide concentration on DBP formation by ozonation or O_(3)-AC treatment were also *** showed that the potential formation of DBPs might be attributed to ozonation,but these DBP precursors could be further removed by activated carbon(AC)***,the formation of target DBPs was controlled at acidic pH by alleviating the reactions between chlorine and ***,the bromide substitution factor(BSF)value of trihalomethanes(THMs)from EOM and IOM remained constant after AC ***,THM precursors could be significantly decreased by AC *** above results indicated that O_(3)-AC was a feasible treatment method for algal-impacted water.

关键词： Ozone combined with activated carbon treatment Chlorella sp. Disinfection byproducts PH Bromide substitution factor

Experimental Study on Impingement and Film Overall Cooling Characteristics of Turbine Shroud

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Journal of Thermal Science 2022年第1期31卷 239-250页

作者： LI Ziqiang WANG Longfei MAO junkui BI Shuai WANG Feilong College of Energy and Power Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China

Based on the variable characteristics of the actual operating conditions of the turbine shroud and the purpose of improving the cooling effect of the turbine shroud,this paper builds a test system of the impingement-film cooling shroud with two gas inlet angles(90°,167°).The effects of film cooling hole arrangement,gas inlet angle,blowing ratio(0.7,1.0,1.5,2.0,2.5,3.0)and temperature ratio(1.2,1.3,1.4,1.5,1.6)on the cooling characteristics of the impingement-film cooling shroud were experimentally studied by infrared temperature measurement technology,especially the effects of gas inlet angle and temperature *** results showed that the film covering effect of the film cooling hole vertical or the same direction of the high-temperature gas incoming flow is better than the film covering effect of the reverse direction with the incoming flow,and the optimal arrangement of film cooling holes can improve the cooling effectiveness of the *** with 90°intake gas,the film coverage area on the shroud surface of the 167°intake gas is expanded,and the surface average overall cooling effectiveness is increased by 1.03%to 12.6%.The overall cooling effectiveness of turbine shroud increases with the increase of blowing ratio,which increases the flow rate and pressure of cooling gas,and the corresponding increase rate is between 1.04%and 9.96%.However,the increase in the temperature ratio increases the mainstream heating capacity,resulting in a decrease in the cooling effectiveness of the shroud,with a maximum reduction rate of 11.04%.

关键词： turbine shroud overall cooling effectiveness impingement-film cooling blowing ratio temperature ratio

Multi-scale collaborative design method for macroscopic thermal optimization and mesoscopic woven structure of hypersonic vehicle's TOCMC leading edge

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Chinese Journal of Aeronautics 2024年第4期37卷 524-541页

作者： Chenwei ZHAO Zecan TU junkui MAO Jian HUI Pingting CHEN College of Energy and Power Engineering Nanjing University of Aeronautics and AstronauticsNanjing 21016China Integrated Energy Institute Nanjing University of Aeronautics and AstronauticsNanjing 210016China

A new thermal protection design method for hypersonic vehicle's leading edge is proposed, which can effectively reduce temperature of the leading edge without additional cooling measures. This method reduces the leading-edge's temperature by the multi-scale collaborative design of the macroscopic thermal optimization and the mesoscopic woven structures of Three-dimensional Orthogonal Woven Ceramic Matrix Composites(TOCMC). The macroscopic thermal optimization is achieved by designing different mesoscopic woven structures in different regions to create combined heat transfer channels to dredge the heat. The combined heat transfer channel is macroscopically represented by the anisotropic thermal conductivity of TOCMC. The thermal optimization multiple linear regression model is established to optimize the heat transport channel, which predicts Theoretical Optimal Thermal Conductivity Configuration(TOTCC) in different regions to achieve the lowest leading-edge temperature. The function-oriented mesostructure design method is invented to design the corresponding mesostructure of TOCMC according to the TOTCC, which consists of universal thermal conductivity prediction formulas for TOCMC. These universal formulas are firstly derived based on the thermal resistance network method, which is verified by experiments with an error of 6.25%. The results show that the collaborative design method can effectively reduce the leading edge temperature by about 12.8% without adding cooling measures.

关键词： Multi-scale collaborative design Thermal optimization Ceramic matrix composite Hypersonic vehicle Thermal protection Intelligent optimization

突变型hTNFα重组体的构建及其在真核细胞中的表达

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微生物学免疫学进展 1998年第4期26卷 10-12页

作者：艾军魁孟筱琦陈一戎秦大山王兴民王志平白淑芳刘国栋兰州医学院第二附属医院泌尿外科研究所卫生部兰州生物制品研究所

选择一种高效低毒的ｈＴＮＦαｃＤＮＡ突变体为目的基因，以质粒ｐｃＤＮＡ３１（＋）为载体，构建了ｈＴＮＦα重组体ｐｃＤＮＡ３１（＋）－ｈＴＮＦα。经限制性内切酶分析证实了重组体结构的正确性，用ＤＮＡ－磷酸钙共沉淀法... 详细信息

选择一种高效低毒的ｈＴＮＦαｃＤＮＡ突变体为目的基因，以质粒ｐｃＤＮＡ３１（＋）为载体，构建了ｈＴＮＦα重组体ｐｃＤＮＡ３１（＋）－ｈＴＮＦα。经限制性内切酶分析证实了重组体结构的正确性，用ＤＮＡ－磷酸钙共沉淀法将重组体导入鼠成纤维细胞ＮＩＨ３Ｔ３中，测其瞬时表达，证明重组体有表达突变型ｈＴＮＦα蛋白的功能。突变型ｈＴＮＦα表达质粒的成功构建。

关键词：肿瘤坏死因子α 突变体重组体构建转染