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Development of a PCR-based method to monitor arthropod dispersal in agroecosystems:Macrolophus pygmaeus(Hemiptera:Miridae)from banker plants to tomato crops

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Insect Science 2020年第5期27卷 1125-1134页

作者： Nuria agusti Cristina Castane Irene Fraile Oscar Alomar IRTA(Institute of Agrifood Research and Technology) CabrilsBarcelonaSpain

Development of conservation biological control programs requires the identifi-cation of sources that contribute to predator colonization of crops.Macrolophus pygmaeus(Rambur)(Hemiptera:Miridae)is an efficient polyphagous predator used in biological con-trol programs in vegetable crops in Europe.We have developed a marking method based on spraying with a solution of the brine shrimp Artemia spp.(Anostraca:Artemidac)cysts,followed by a PCR detection of Artemia DNA to monitor M.pygmaeus dispersal from banker plants to tomato crops.Experiments conducted in climatic chambers show that the topical application of this marking solution on M.pygmaeus does not significantly reduce adult longevity and that it is detected up to 6 d after the application.When this Artemia solution was applied on Calendula officinalis L.banker plants harboring M.pygmaeus and maintained outdoors,Artemia DNA was still detected on 62%of the insects after6 d.The conducted field applications in commercial greenhouses have confirmed the usefulness of this method to monitor M.pygmaeus dispersal from banker plants to a newly planted tomato crop.This method can be used to assess arthropod movement,being an interesting molecular approach for further improving future pest management strategies.

关键词： Artemia arthropod dispersal Calendula officinalis Macrolophus pygmaeus PCR analysis tomato crop

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GOLD2017：建议与问题

中华医学杂志 2017年第38期97卷 2963-2966页

作者： Alvar agusti 西班牙巴塞罗那大学医院呼吸研究所西班牙呼吸疾病生物医学研究网络中心 08036

慢性阻塞性肺疾病（简称慢阻肺）全球倡议（GOLD）于1998年由美国国立心肺和血液研究所（NHLBI）和世界卫生组织（WHO）共同成立，并于2001年颁布了第一版慢阻肺诊断、处理和预防全球策略（GOLD2001），其目标是根据已发表的最新研究结... 详细信息

慢性阻塞性肺疾病（简称慢阻肺）全球倡议（GOLD）于1998年由美国国立心肺和血液研究所（NHLBI）和世界卫生组织（WHO）共同成立，并于2001年颁布了第一版慢阻肺诊断、处理和预防全球策略（GOLD2001），其目标是根据已发表的最新研究结果制订慢阻肺的防治管理推荐策略。尽管不是临床指南，但该策略已成为各个国家或地区制定慢阻肺临床指南的重要参考依据。GOLD科学委员会每年对该策略进行年度更新，并分别于2006、2011年进行过两次重大修订。目前，GOLD2017版已经发布，并在定义，诊断评估和治疗等多方面进行了修订和更新。我们有幸邀请到GOLD理事会现任主席、西班牙巴塞罗那大学医院呼吸研究所的Alvaragusti教授撰写此文，就GOLD2017与中国呼吸界医师分享一些他个人的观点。本文由四川大学华西医院呼吸与危重症医学科安静医师翻译、文富强教授审校，并在译文后附有Alvaragusti教授的原文供大家参考与借鉴。

关键词：慢性阻塞性肺疾病四川大学华西医院血液研究所世界卫生组织临床指南 GOLD 诊断评估危重症医学

Analysis and Design of Vector Control for VSC-HVDC Connected to Weak Grids

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CSEE Journal of Power and Energy Systems 2017年第2期3卷 115-124页

作者： Guanglu Wu Jun Liang Xiaoxin Zhou Yalou Li agusti Egea-Alvarez Gen Li Hongying Peng Xing Zhang State Key Laboratory of Power Grid Safety and Energy Conservation(China Electric Power Research Institute) Beijing 100192China School of Engineering Cardiff UniversityCardiffCF243AAUK

Voltage source converter based high voltage direct current transmission(VSC-HVDC)is considered one of the most suitable technologies to integrate renewable energies.However,connecting VSC to a weak grid is challenging since traditional vector control tends to become unstable under high power demand conditions.In this paper,an improved vector control method is proposed wherein a feed forward branch based on steady state and small signal analysis of the VSC system is added under weak grid situations.The feed forward branch promotes faster reactive power response,thus enhancing the stability of the VSC system.Since the improved vector control uses the same inner loop as traditional vector control,the proposed method allows for the ability to retain fault current suppression capabilities.Furthermore,the control parameters of the outer loop of the improved vector control need not vary according to the variation of the operating points,which makes it easy to implement.The feed forward branch is implemented by solving a nonlinear equation or through use of a look-up table.The influence of the estimation errors of short circuit ratio(SCR)on the control performance is also studied.The effectiveness of the improved vector control is demonstrated through small signal model analysis and time domain simulations.

关键词： Improved vector control VSC-HVDC weak AC grids

弱交流电网条件下VSC-HVDC改进矢量控制方法

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中国电机工程学报 2017年第16期37卷 4577-4590页

作者：吴广禄周孝信李亚楼梁军 agusti Egea-Alvarez 彭红英张星电网安全与节能国家重点实验室(中国电力科学研究院) 北京市海淀区100192 卡迪夫大学

当电压源换流器型直流输电(voltage source converter based high voltage direct current transmission,VSC-HVDC)联接弱交流电网输送功率接近交流电网静态稳定极限时,经典矢量控制方法无法保证换流器稳定运行。文中分析VSC联接于弱交... 详细信息

当电压源换流器型直流输电(voltage source converter based high voltage direct current transmission,VSC-HVDC)联接弱交流电网输送功率接近交流电网静态稳定极限时,经典矢量控制方法无法保证换流器稳定运行。文中分析VSC联接于弱交流电网时的潮流特性,研究经典矢量控制外环在弱交流电网条件下的特性,通过基于小信号模型的极点分析研究导致换流器无法稳定运行的原因,基于VSC联接于弱交流电网时的失稳机理提出一种改进矢量控制方法,在矢量控制外环增加前馈支路,增加暂态无功响应速度,提高弱交流电网条件下VSC在输送功率接近交流电网静态稳定极限时的稳定性。最后,基于小信号建模的极点分析和时域仿真结果验证改进矢量控制方法的有效性。

关键词：电压源换流器直流输电弱交流电网改进矢量控制

初榨橄榄油中多酚化合物的UPLC-FLD检测及其抗氧化活性研究

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浙江农业学报 2021年第5期33卷 907-915页

作者：李雪张玉王君虹朱作艺孙素玲刘志朱申龙沈国新 agusti ROMERO 王伟浙江省农业科学院农产品质量安全与营养研究所浙江杭州310021 农业农村部农产品信息溯源重点实验室浙江杭州310021 木本油料品质营养国际联合实验室浙江杭州310021 湖南人文科技学院农业与生物技术学院湖南娄底417000 西班牙加泰罗尼亚农业与食品科技研究所西班牙加泰罗尼亚08140

采用超高效液相色谱荧光检测法(UPLC-FLD)检测不同品种初榨橄榄油中6种多酚化合物的含量,并测定样品的总多酚含量及抗氧化活性,同时对多酚组分与抗氧化能力的相关性进行分析。结果表明:色谱条件为采用Cadenza色谱柱(150 mm×4.6 mm,5μ... 详细信息

采用超高效液相色谱荧光检测法(UPLC-FLD)检测不同品种初榨橄榄油中6种多酚化合物的含量,并测定样品的总多酚含量及抗氧化活性,同时对多酚组分与抗氧化能力的相关性进行分析。结果表明:色谱条件为采用Cadenza色谱柱(150 mm×4.6 mm,5μm),柱温35℃,以0.1%甲酸-水(A)-乙腈(B)为流动相进行梯度洗脱,荧光光谱激发波长为278 nm,发射波长为339 nm。在此条件下,初榨橄榄油样品中6种多酚化合物可在17 min内得到较好分离,并在较宽的浓度范围内呈良好的线性关系(R2≥0.9995),且精密度高,日内精密度相对标准偏差(RSD)≤4.91%,日间精密度RSD≤5.41%,加标回收结果准确可靠,平均回收率93.42%~119.86%,RSD≤6.26%。不同初榨橄榄油样品中羟基酪醇、酪醇、香草酸、松脂酚及总多酚含量差异较大,而橄榄苦苷和女贞苷含量最低,且样品间差异较小。初榨橄榄油多酚提取物具有良好的抗氧化活性,但不同品种间具有较大差异,ABTS法测定的总抗氧化能力为0.95~2.71 mmol·L^(-1) Trolox,DPPH自由基清除率为7.22%~66.23%,抑制羟自由基能力为13.24~136.06 U·mL^(-1),其中Coratina的抗氧化活性最强,总多酚含量与提取物总抗氧化能力呈显著正相关。

关键词：初榨橄榄油多酚化合物超高效液相色谱荧光检测法抗氧化活性

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环剥对柑桔大小年结果的影响

国外农学（果树） 1992年第4期 23-26页

作者： M.agusti 聂华堂

环剥二级分枝增加了柑桔开花。环剥能使萌芽数增加,但其效果依环剥日期而定,夏季环剥效果最佳。除营养枝外,环剥能使其它各类枝数量增加,但枝梢特性不变。表现大小年结果的品种,如沙鲁斯梯阿纳甜橙。

关键词：柑桔环剥结果产量

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肝硬化患者的肺部疾患

国外医学（消化系疾病分册） 1991年第1期11卷 29-30页

作者： agusti AGN 翁林

肝硬化患者可发生各种肺部疾患,约1/3肝硬化者可在无明显的心肺疾病情况下发生轻中度低氧血症。近两年来,对肝-肺综合征的病理生理基础已有所认识。历史背景入肺静脉血不能充分氧含的原因为:1.肺换气不足可以排除,一般是换气过度;2.肺... 详细信息

肝硬化患者可发生各种肺部疾患,约1/3肝硬化者可在无明显的心肺疾病情况下发生轻中度低氧血症。近两年来,对肝-肺综合征的病理生理基础已有所认识。历史背景入肺静脉血不能充分氧含的原因为:1.肺换气不足可以排除,一般是换气过度;2.肺动静脉分流曾是肝硬化者低氧血症的“经典”解释,这一发现产生了三种不同的假设,a.静脉血能通过肺内动静脉交通,b.肝血管在前毛细血管水平呈大而明显的扩张,c.在门脉系统和肺血管系统间存在交通,虽然某些动静脉分流的存在有助于解释肝硬化者低氧血症,但必定尚有其他因素存在;3.

关键词：肝硬变肺部疾病肝-肺综合征

Pedigree analysis of 220 almond genotypes reveals two world mainstream breeding lines based on only three different cultivars

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Horticulture Research 2021年第1期8卷 72-82页

作者： Felipe Perez de los Cobos Pedro J.Martinez-Garcia agusti Romero Xavier Miarnau Iban Eduardo Werner Howad Mourad Mnejja Federico Dicenta Rafel Socias i Company Maria J.Rubio-Cabetas Thomas M.Gradziel Michelle Wirthensohn Henri Duval Doron Holland Pere Arus Francisco J.Vargas Ignasi Batlle Institut de Recerca i Tecnologia Agroalimentaries(IRTA) Mas BoveCtra.Reus-El Morell Km 3843120 ConstantiTarragonaSpain Institut de Recerca i Tecnologia Agroalimentaries(IRTA) Centre de Recerca en Agrigenomica(CRAG)CSIC-IRTA-UAB-UB.Cerdanyola del Valles(Bellaterra)08193 BarcelonaSpain Centro de Edafologia y Biologia Aplicada del Segura Consejo Superior de Investigaciones Cientificas(CEBAS-CSIC)P.O.Box 16430100 EspinardoMurciaSpain Institut de Recerca i Tecnologia Agroalimentaries(IRTA) FruitcentrePCiTALGardeny ParkFruitcentre Building25003 LleidaSpain Centro de Investigacion y Tecnologia Agroalimentaria de Aragon(CITA) Avda.Montanana 93050059ZaragozaInstituto Agroalimentario de Aragon IA2(CITA-Universidad de Zaragoza)ZaragozaSpain University of California 1 Shields AvenueDavisCA 95616USA University of Adelaide Waite ResearchSchool of AgricultureFood and WinePMB 1Glen OsmondAdelaideSA 5064Australia Institut National de la Recherche Agronomique(INRA) Domain St.Maurice CS 6009484143 Montfavet CedexFrance Agricultural Research Organization Newe-Ya’ar Research CenterP.O.Box 1021Ramat Yishad 30095Israel

Loss of genetic variability is an increasing challenge in tree breeding programs due to the repeated use of a reduced number of founder genotypes.However,in almond,little is known about the genetic variability in current breeding stocks,although several cases of inbreeding depression have been reported.To gain insights into the genetic structure in modern breeding programs worldwide,marker-verified pedigree data of 220 almond cultivars and breeding selections were analyzed.Inbreeding coefficients,pairwise relatedness,and genetic contribution were calculated for these genotypes.The results reveal two mainstream breeding lines based on three cultivars:“Tuono”,“Cristomorto”,and“Nonpareil”.Descendants from“Tuono”or“Cristomorto”number 76(sharing 34 descendants),while“Nonpareil”has 71 descendants.The mean inbreeding coefficient of the analyzed genotypes was 0.041,with 14 genotypes presenting a high inbreeding coefficient,over 0.250.Breeding programs from France,the USA,and Spain showed inbreeding coefficients of 0.075,0.070,and 0.037,respectively.According to their genetic contribution,modern cultivars from Israel,France,the USA,Spain,and Australia trace back to a maximum of six main founding genotypes.Among the group of 65 genotypes carrying the S f allele for self-compatibility,the mean relatedness coefficient was 0.125,with“Tuono”as the main founding genotype(24.7%of total genetic contribution).The results broaden our understanding about the tendencies followed in almond breeding over the last 50 years and will have a large impact into breeding decision-making process worldwide.Increasing current genetic variability is required in almond breeding programs to assure genetic gain and continuing breeding progress.

关键词： breeding cultivar founding

阻塞性睡眠呼吸暂停综合征男性患者持续正压通气治疗对心血管系统远期预后的疗效观察

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世界核心医学期刊文摘（神经病学分册） 2005年第9期1卷 5-6页

作者： Marin J.M. Carrizo S.J. Vicente E. agusti A.G.N. 黄卫东 Servicio de Neurologia Hospital Universitario Miguel Servet Isabel la Catolica 50006 Zaragoza SpainDr.

Background: The effect of obstructive sleep apnoea-hypopnoea as a cardiovascular risk factor and the potential protective effect of its treatment with continuous positive airway pressure (CPAP) is unclear. We did an observational study to compare incidence of fatal and non-fatal cardiovascular events in simple snorers, patients with untreated obstructive sleep apnoea-hypopnoea, patients treated with CPAP, and healthy men recruited from the general population. Methods: We recruited men with obstructive sleep apnoea-hypopnoea or simple snorers from a sleep clinic, and a population-based sample of healthy men, matched for age and body-mass index with the patients with untreated severe obstructive sleep apnoea-hypopnoea. The presence and severity of the disorder was determined with full polysomnography, and the apnoea-hypopnoea index (AHI) was calculated as the average number of apnoeas and hypopnoeas per hour of sleep. Participants were followed-up at least once per year for a mean of 10.1 years (SD 1.6) and CPAP compliance was checked with the built-in meter. Endpoints were fatal cardiovascular events (death from myocardial infarction or stroke) and non-fatal cardiovascular events (non-fatal myocardial infarction, non-fatal stroke, coronary artery bypass surgery, and percutaneous transluminal coronary angiography). Findings: 264 healthy men, 377 simple snorers, 403 with untreated mild-moderate obstructive sleep apnoea-hypopnoea, 235 with untreated severe disease, and 372 with the disease and treated with CPAP were included in the analysis. Patients with untreated severe disease had a higher incidence of fatal cardiovascular events (1.06 per 100 person-years) and non-fatal cardiovascular events (2.13 per 100 person-years) than did untreated patients with mild-moderate disease (0.55, p=0.02 and 0.89, p < 0.0001), simple snorers (0.34, p=0.0006 and 0.58, p < 0.0001), patients treated with CPAP (0.35, p=0.0008 and 0.64, p < 0.0001), and healthy participants (0.3, p=0.0012 and 0.45, p < 0.0001). Multivariate analysis, a

关键词：正压通气治疗心血管系统呼吸暂停指数持续正压通气 CPAP 多导睡眠记录仪健康受试者非致死性心血管事件中度患者