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Production and Partial Characterization of an Extracellular Phytase Produced by Muscodor sp. under Submerged Fermentation

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Advances in Microbiology 2016年第1期6卷 23-32页

作者： Natálie Martins Alves Luis Henrique Souza Guimarães Roberta Hilsdorf piccoli Patrícia Gomes Cardoso Department of Biology Sector of Microbiology Federal University of Lavras Lavras Brazil Department of Biology University of Sao Paulo Ribeirao Preto Brazil Department of Food Science Federal University of Lavras Lavras Brazil

In most of the raw materials of plant origin used in animal feed, a portion of the phosphorus is stored as phytic acid or phytate. Phytate is the main storage form of phosphorus in vegetables but is not readily assimilated into food at low concentrations of the enzyme phytase. In addition to making phosphorous unavailable, phytate binds divalent cations such as calcium, copper, magnesium, iron, manganese and zinc, preventing the absorption of these nutrients in the gut of the animal. Phytase promotes the hydrolysis of the phytate phosphorus-releasing molecule, thereby increasing its bioavailability in feed. Phytase is distributed in plant and animal tissues and it is synthesized by some species of bacteria and fungi. The addition of this enzyme in the diet of animals is essential to promote greater uptake of phosphorus and also contributes to a decrease in the levels of phosphorus excreted by animals, thus reducing the pollution caused by excess phosphorus in the environment. This work aimed to select a fungus that stands out in the production of phytase among 100 isolates from Brazilian caves belonging to the genera Aspergillus, Penicillium and Cladosporium and 13 endophytic fungi of the aerial part of the coffee plant. For selection, the fungi were cultured in medium containing phytic acid as a sole source of phosphorus. After seven days at 25 °C, we evaluated growth and enzyme production by the presence of the phytic acid halo degradation (Enzymatic Index-EI) surrounding the colonies. Forty-seven species produced phytase, and the fungi Penicillium minioluteum (CF279) and Muscodor sp. (UBSX) showed higher degradation halos, 2.41 and 4.46, respectively. Considering the Muscodor sp. as the main source of phytase, high enzymatic levels were obtained when the fungus was grown under submerged fermentation with initial pH of 5.0 using wheat bran as additional carbon source for 144 h, at 125 rpm and 30 °C. Additionally, the enzyme was stable at pH 5.0 and 40 °C, and inhi

关键词： Endophytic Fungus Muscodor sp. Phosphorus Phytase Phytic Acid

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用于敞开式变电站的特高压开关设备

华东电力 2007年第C期35卷 104-113页

作者： Francois Biquez Denis Chatrefou Jean-Marie Delcoustal Ferruccio Dianin Christian Lindner Raoul piccoli AREVA T&D France

作为1100kV的UHV（特高压）输电，面临技术——生态环境的巨大挑战。变电站的UHV开关装置模块，需要的是可靠、成本／效益比好、环境友好、可维护的方案。对于这些所需的参数以及技术规范的验证，采用AIS（空气隔离变电所）技术能完全... 详细信息

作为1100kV的UHV（特高压）输电，面临技术——生态环境的巨大挑战。变电站的UHV开关装置模块，需要的是可靠、成本／效益比好、环境友好、可维护的方案。对于这些所需的参数以及技术规范的验证，采用AIS（空气隔离变电所）技术能完全满足这些需要。介绍由瓷柱式断路器、隔离开关和NCIT（非传统互感器）所组成的UHV模块。这些装置是根据模块原理预先设计好的，其中的设备间的连接肯定允许快速安装。在变电站设备控制和检测方面，模块是预制标准化。加拿大和德国的试验站对断路器、隔离开关等独立的开关元件，在1100kV上做BIL、SIL和工频等绝缘试验，性能表现非常优越。因为对于特高压电压等级，IEC标准至今尚未规定，所以将根据外推法和现场数据作合理解释。瓷柱式断路器采用了最新复合绝缘体技术，在安全方面有所改进。AIS方案其他的关键技术，例如环境保护和成本，在可比的功能间，根据市场估计价值，与GIS解决方法作了比较。

关键词：特高压敞开式变电站开关设备

Expression of CD55, CD59, CD46 and CD35 in Peripheral Blood Cells from Rheumatoid Arthritis Patients

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Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 69-73页

作者： Amanda Kirchner piccoli Ana Paula Alegretti Laiana Schneider Priscila Schmidt Lora Claiton Viegas Brenol Ricardo Machado Xavier Division of Rheumatology Hospital de Clínicas de Porto Alegre Porto Alegre Brazil. Divison of Clinical Pathology Hospital de Clínicas de Porto Alegre Porto Alegre Brazil Divison of Clinical Pathology Hospital de Clínicas de Porto Alegre Porto Alegre Brazil

Evidences point that complement activation plays a role in rheumatoid arthritis (RA). In this context, expression of the CReg was investigated on white cells from peripheral blood of 30 RA patients and 30 healthy controls. Using flow cytometric analyses the relative fluorescence intensities (MFI) of Cregs were determined. CD59 MFI was significantly increased in RA cells comparing to controls, respectively: lymphocytes 36.8 versus 27.07;monocytes 32.0 versus 21.37;and granulocytes: 84.6 versus 66.1 (p 0.05). Interestingly, no difference was observed on the MFI to CD55, CD46 and CD35 in these cells. These data indicate an overexpression of CD59 in all the peripheral blood cells of RA patients, perhaps due to an increased synthesis for compensatory mechanisms because of complement activation, inflammatory status or other factors associated with the disease.

关键词： Rheumatoid Arthritis Complement System Complement Regulatory Protein Flow Cytometry

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特殊用途胶管的设计

世界橡胶工业 1980年第3期 69-74页

作者： D.E.piccoli 赵光贤

引言在讨论特殊用途胶管的设计之前,宜先重温一下胶管结构的各部件、基本增强方法以及骨架材料。胶管可以认为是一种挠性的导管,用来容纳流体或把它从一处输往另一处;或用来以液威气动方式传递能量。胶管与刚性导管相比,具有如下一些优... 详细信息

引言在讨论特殊用途胶管的设计之前,宜先重温一下胶管结构的各部件、基本增强方法以及骨架材料。胶管可以认为是一种挠性的导管,用来容纳流体或把它从一处输往另一处;或用来以液威气动方式传递能量。胶管与刚性导管相比,具有如下一些优点:富有柔曲性、吸振、阻声、安装简易、耐腐蚀、耐磨。

关键词：胶管特殊用途挠性编织角钢缆钢丝帘布力学性能外胶层内胶层

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2018世界肾脏日：关注女性和肾脏疾病

肾脏病与透析肾移植杂志 2018年第1期27卷 1-6页

作者： Giorgina B.piccoli Mona Alrukhaimi Zhi-Hong Liu Elena Zakharova Adeera Levin Philip Kam Tao Li Guillermo Garcia-Garcia Mohammed Benghanem-Gharbi Kamyar Kalantar-Zadeh Charles Kernahan Latha Kumaraswami Giorgina Barbara piccoli Gamal Saadi Louise Fox Sharon Andreoli 陈樱花 Department of Clinical and Biological Sciences University of Torino Nephrology Centre Hospitalier Le Mans Department of Medicine Dubai Medical College National Clinical Research Center of Kidney Diseases Jinling Hospital Nanjing University School of Medicine Nephrology Moscow City Hospital n.a. S.P. Botkin Nephrology Moscow State University of Medicine and Dentistry Nephrology Russian Medical Academy of Continuous Professional Education the World Kidney Day Steering Committee Department of Medicine Division of NephrologyUniversity of British Columbia 国家肾脏疾病临床医学研究中心南京总医院

女性是现代家庭和社会的重要贡献者。但目前在世界范围内,男性和女性在受教育程度、医疗保健等方面仍存在显著差异。2018年第13个世界肾脏日恰逢国际妇女节,其主题是"肾脏和女性健康",给我们提供了一个关注女性健康,尤其是女性肾脏健康... 详细信息

女性是现代家庭和社会的重要贡献者。但目前在世界范围内,男性和女性在受教育程度、医疗保健等方面仍存在显著差异。2018年第13个世界肾脏日恰逢国际妇女节,其主题是"肾脏和女性健康",给我们提供了一个关注女性健康,尤其是女性肾脏健康的重要契机。为此,世界肾脏日指导委员会(World Kidney Day Steering Committee)特别组织了全球具有代表性的5位女性肾脏病学家,撰写了述评文章,借机呼吁肾脏病学术领域和全社会更加重视女性健康,让更多女性从肾脏病学术研究中获益。关注女性肾脏健康,利在当代功在千秋!

关键词：女性健康肾脏疾病世界范围肾脏病学学术研究国际妇女节现代家庭医疗保健

Liver dysfunction markedly decreases the inhibition of CYP1A2-mediated theophylline metabolism by fluvoxamine

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Liver dysfunction markedly decreases the inhibition of CYP1A...

Homologous and Heterologous Adaptation of Listeria *** Essential Oils of Condiment Plants

第十五届国际药理学大会

作者： P. Palatini S. De Martin P. piccoli R. Padrini R. Orlando Department of Pharmacology and Anaesthesiology Department of Pharmacology and Anaesthesiology Department of Pharmacology and Anaesthesiology Department of Pharmacology and Anaesthesiology Department of Medical and Surgical Sciences University of PadovaItaly.

＜正＞ Objectives: To evaluate the effect of cirrhosis on the inhibition by fluvoxamine of theophylline metabolism, to assess whether liver dysfunction has any influence of drug interaction involving CYP1A2. Methods: The study was carried out in 10 healthy volunteers, 10 patients with Child A, and 10 with Child C cirrhosis, according to a randomized, double - blind, 2 - phase, crossover design. Results: Fluvoxamine - induced inhibition of theophylline clearance decreased from 62 %. in controls, to 52% and 12% in Child A and C cirrhotics, respectively. CYP1A2 - mediated formations of 3 - methylxanthine and 1 - methyluric acid were totally inhibited in controls, but reduced by only one third in Child C cirrhotics . Inhibition of 1,3 - dimethyluric acid formation decreased from 58% , in controls, to 43% and 7% in patients with Child grade A and C cirrhosis, respectively. Conclusions: Two mechanisms are proposed to explain the attenuating effect of cirrhosis on CYP1A2 inhibition: decreased sensitivity to fluvoxamine of CYP1A2 - mediated biotransformations. probably due to reduced uptake of the inhibitory drug by the cirrhotic liver;reduced hepatic expression of CYP1A2, which makes its inhibition less important.

关键词： theophylline - fluvoxamine interaction liver disease

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Advances in Microbiology 2018年第8期8卷 639-649页

作者： Jéssica M.P.Santos Michelle C.Goncalves Heloisa A.Martins Juliana J.Pinelli Silas R.Isidoro Roberta H.piccoli Food Microbiology Laboratory Universidade Federal de LavrasLavrasBrazil Food Science Department Universidade Federal de LavrasLavrasBrazil

The homologous and heterologous adaptation capacity of L. monocytogenes and L. innocua were determined for thyme, oregano and nutmeg essential oils, as well as their adaptation capacities to acidic stress. Minimum bactericidal concentrations (CMB) and minimum inhibitory and minimum growth pH, were established. The capacity for increased tolerance to essential oils and acidic stress, along with heterologous adaptation among the essential oils tested and to acidic pH was determined, and that between pH and essential oils. L. monocytogenes and L. innocua adapted to all essential oils and to the minimum inhibitory pH, after exposition to sub-lethal conditions. Both strains presented heterologous adaptation capacity. After previous exposition to sub-lethal essential oil concentrations, the regenerated cells were capable of growth under 3.5 pH values, and increased CMB values. Essential oil CMBs for previously cultivated cells under minimum growth pH environments also increased, attaining values 1.6 times superior to previous ones.

关键词： Cross-Adaptation Natural Antimicrobial L. monocytogenes Tolerance

NOTCH2 Mutations Cause Alagille Syndrome,a Heterogeneous Disorder of the Notch Signaling Pathway

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NOTCH2 Mutations Cause Alagille Syndrome,a Heterogeneous Dis...

SS-LASS ICP-MS锆石年代学新技术对有限流体条件下深俯冲榴辉岩变质年龄的制约

第二届走向前沿——IFCC国际高级生化和分子细胞生物学暑期培训班

作者： Ryan McDaniell Daniel M.Warthen Pedro A.Sanchez-Lara Athma Pai Ian D.Krantz David A.piccoli Nancy B.Spinner

Alagille syndrome(AGS)is caused by mutations in the gene for the Notch signaling pathway ligand Jaggedl(JAG1),which are found in 94% of *** identify the cause of disease in patients without JAG1 mutations,we screened11 JAG1 mutation-negative probands with AGS for alterations in the gene for the Notch2 receptor(NOTCH2).We found NOTCH2 mutations segregating in two families and identified five affected *** manifestations,a minor feature in AGS,were present in all the affected *** demonstrates that AGS is a heterogeneous disorder and implicates N0TCH2 mutations in human disease.

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SS-LASS ICP-MS锆石年代学新技术对有限流体条件下深俯冲榴辉岩变...