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Value of duplex doppler ultrasonography in non-invasive assessment of children with chronic liver disease

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World Journal of Gastroenterology 2010年第48期16卷 6139-6144页

作者： mortada HF El-Shabrawi maissa El-Raziky maha Sheiba Hanaa m El-Karaksy mona El-Raziky Fetouh hassanin Abeer Ramadan Department of Pediatrics Cairo University Department of Tropical medicine Cairo University Department of Clinical Pharmacy Misr International University

AIm:To investigate the value of duplex Doppler ultrasonography (US) in the assessment of the hemodynamics of the portal and hepatic veins in a cohort of children with chronic liver disease (CLD) and to detect any relationship between the US changes,etiology and severity (or stage) of CLD. mETHODS:We prospectively enrolled 25 children with biopsy-proven CLD. Thirteen had cirrhosis (aged 8.9 ± 2.0 years) and 12 had chronic hepatitis (aged 9.3 ± 2.3 years). Gray scale and color-coded duplex Doppler US were performed for all,as well as 30 healthy age and sex-matched controls. Findings were correlated with clinical,laboratory and histopathological characteristics. RESULTS:Prominent caudate lobe was detected in 100% of cirrhotics,but none of the chronic hepatitis or controls. Thickened lesser omentum and loss of the triphasic waveform of the hepatic vein were present in 69.2% and 53.8% of cirrhotics vs 33.3% and 8.3% of chronic hepatitis respectively. Portal vein flow velocity was significantly lower (P mpared to controls. Child-Pugh's staging showed a positive correlation with both abnormal hepatic vein waveform and direction of portal blood flow; and a negative correlation with both hepatic and portal vein flow velocities. No correlation with the etiology of CLD could be detected. CONCLUSION:Duplex Doppler added to grayscale US can detect significant morphologic and portal hemodynamic changes that correlate with the severity (stage) of CLD,but not with etiology.

关键词： Chronic hepatitis Chronic liver disease Cirrhosis Doppler Grayscale Pediatrics Ultrasound

micropropagation of Daylily(Hemerocallis fulva)from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT markers

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2183-2196页

作者： Esraa E.Shalan Said S.Soliman Ahmed A.mahmoud Jameel m.Al-Khayri Salha m.ALshamrani Fatmah A.Safhi Areej S.Jalal Diaa Abd El-moneim Abdallah A.hassanin Genetics Department Faculty of AgricultureZagazig UniversityZagazig44511Egypt Department of Agricultural Biotechnology College of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityRiyadh11671Saudi Arabia Department of Plant Production (Genetic Branch)Faculty of Environmental and Agricultural SciencesArish UniversityEl-Arish45511Egypt

Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale *** this research,nine multiplication media(mm)augmented with different concentrations of 6-benzyl adenine(BA),Kinetin(Kin),and Thidiazuron(TDZ),Three rooting media(Rm)supplemented with three levels ofα-naphthalene acetic acid(NAA)and three types of soil mixtures(v/v);Coco peat/Vermiculite/Sand(CVS),Peat moss/Perlite/Sand(PPS)and Peat moss/Perlite(PP)were used in the micropropagation protocol of daylily ***2 showed the maximum shoot length and the number of leaves,while mm9 showed the maximum number of *** Rm1 showed the maximum root length and the number of *** acclimatization,CVS,PPS,and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and *** genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted(SCoT)*** SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp *** one out of 51 loci were presented in the mother plants.40 loci were polymorphic,11 were monomorphic and 7 were *** amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway,thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication *** research can be successfully used for other ornamental and medicinal plants’bulk multiplication,germplasm conservation,and future genetic improvement.

关键词： Genetic fidelity medicinal plants micropropagation molecular markers ornamental plants somaclonal variation

Fetal RBCs Potential in Obstetric Protocols to minimize Fetomaternal Hemorrhage

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Open Journal of Blood Diseases 2017年第1期7卷 51-63页

作者： Hasnaa A. Abo-Elwafa Salah A. Ismail Ibrahim m. A. hassanin Rania m. Ahmed Sohag Faculty of medicine Sohag University Sohag Egypt

Background: During pregnancy a small number of fetal RBCs (Red Blood Cells) enter maternal blood without risk, and incidence of significant feto-maternal hemorrhage (FmH) and isoimmunization depends on how much fetal blood enters maternal circulation;the use of flow cytometer (FCm) in FmH detection is considered the best laboratory technique. Aims: To evaluate role of FCm in FmH estimation to optimize delivery protocols and decrease isoimmunization. Subject and method: 100 pregnant women at labor were included, equally classified into early cord clamping (ECC) and delayed cord clamping (DCC) groups, each including 25 women delivered vaginally and 25 with caesarian section;the control groups included 20 non-pregnant females, 20 at 3rd trimester and 20 neonates. Fetal RBCs were done on Becton Dickinson (B.D) FCm, and the reagents used were 5-Glutaraldehyde 0.5% Sigma cat. G5882, flouroscine isothiocyanite (FITC) anti-hemoglobin F (Hb-F) cat. 555748, anti-carbonic anhydrase, Triton-X100 solution and Alsever’s solution cat. HFH-11, 0.1% phosphate buffer saline/bovine serum albumin (PBS/BSA). Statistical Analysis: mann Whitney’s U-test, Chi-squared, Fischer’s Exact tests, and SPSS for windows version 15.0. Results: There was a significant increase in the volume of the FmH in the ECC compared to DCC groups (p mparing to caesarian section subgroups within the DCC group (p mpared to DCC within caesarian section subgroup (p mation by FCm is good;perinatal laboratory test should be done in routine investigations, as a guide for obstetric techniques that can alter FmH volume and decrease subsequent isoimmunization.

关键词： Fetomaternal Hemorrhage Obstetric Protocols Flow-Cytometer

Pre-Breeding Genetic Diversity Assessment of Tomato(Solanum lycopersicum L.)Cultivars Based on molecular,morphological and Physicochemical Parameters

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1493-1512页

作者： Jameel m.Al-Khayri Salha m.Alshamrani Adel A.Rezk Wael F.Shehata mustafa I.Almaghasla Tarek A.Shalaby Ahmed m.Saad Fatmah A.Safhi muhammad N.Sattar Arafat A.Abdel Latef mahmoud Z.Sitohy Abdallah A.hassanin Department of Agricultural Biotechnology College of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Plant Pests and Diseases UnitDepartment of Arid Land AgricultureCollege of Agriculture and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Horticulture Faculty of AgricultureKafrelsheikh UniversityKafr El-Sheikh33516Egypt Department of Biochemistry Faculty of AgricultureZagazig UniversityZagazig44511Egypt Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Central Laboratories King Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Botany and microbiology Faculty of ScienceSouth Valley UniversityQena83523Egypt Department of Genetics Faculty of AgricultureZagazig UniversityZagazig44511Egypt

Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding *** study characterized fruit yield,quality attributes,and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse *** 86,Castle Rock,and Red Star cultivars showed the highest fruit yield(kg/plant),total phenolic compounds(TPC),and sap *** component analysis categorized the evaluated fruit yield into three groups based on their quality attributes.A robust positive correlation appeared among traits inside each group.A positive correlation was likewise noticed between the first and the second ***,a negative correlation was detected between the first,the second and the third *** profiling,using seven inter-simple sequence repeat(ISSR)primers,produced 60 loci,including 49 polymorphic *** molecular analysis also pinpointed the highest genetic similarity(0.92)between P73 and moneymaker,while the lowest genetic similarity(0.46)was observed between Castle Rock and *** cultivars P73 and moneymaker showed the lowest genetic distance(2.24),while the highest genetic distance(5.92)was observed between Super marmand and Peto86,on the one hand,and between Castle Rock and moneymaker,on the other *** chemical analysis of fruit sap indicated the highest levels of TPC,total flavonoids,anthocyanin,ascorbic acid and total soluble solids in Peto 86 and Castle Rock *** analysis of tomato cultivars based on morphological and molecular attributes indicated four distinct *** 86,Castle Rock,and Red star cultivars can be recommended for the tomato hybridization breeding programs in the future,with other tomato cultivars as potentially high-yielding parents.

关键词： Fruit quality fruit yield genetic diversity hybrid seeds molecular markers plant breeding tomato

Evolution of an avian H5N1 influenza A virus escape mutant

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World Journal of Virology 2013年第4期2卷 160-169页

作者： Kamel m A hassanin Ahmed S Abdel-moneim Biochemistry Department Faculty of Veterinary Medicine Beni-Suef UniversityBeni-Suef 62511Egypt Virology Department Faculty of Veterinary Medicine Beni-Suef UniversityBeni-Suef 62511 Egypt microbiology Department Virology Division College of Medicine Taif UniversityAl-Taif 21944Makkah Province Saudi Arabia

AIm: To investigate the genetic constitution of an escape mutant H5N1 strain and to screen the presence of possible amino acid signatures that could differentiate it from other Egyptian H5N1 ***: Phylogenetic, evolutionary patterns and amino acid signatures of the genes of an escape mutant H5N1 influenza A virus isolated in Egypt on 2009 were analyzed using direct sequencing and multisequence ***: All the genes of the escape mutant H5N1 strain showed a genetic pattern potentially related to Eurasian lineages. Evolution of phylogenetic trees of different viral genes revealed the absence of reassortment in the escape mutant strain while confirming close relatedness to other H5N1 Egyptian strains from human and avian species. A variety of amino acid substitutions were recorded in different proteins compared to the available Egyptian H5N1 strains. The strain displayed amino acid substitutions in different viral alleles similar to other Egyptian H5N1 strains without showing amino acid signatures that could differentiate the escape mutant from other Egyptian ***: The genetic characteristics of avian H5N1 in Egypt revealed evidence of a high possibility of inter-species transmission. No amino acid signatures were found to differentiate the escape mutant H5N1 strain from other Egyptian H5N1 strains.

关键词： Chicken genotyping H5N1 Influenza virus evolution