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Application of Natural Antioxidants from Strawberry Tree(Arbutus unedo L.) and Dog Rose(Rosa canina L.) to Frankfurters Subjected to Refrigerated Storage

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Journal of Integrative Agriculture 2013年第11期12卷 1972-1981页

作者： mónica a.m.nteros David morcuende Sonia Ventanas mario estévez Animal Production and Food Science University of ExtremaduraAv.de la Universidad s/n

The effect of the addition of natural antioxidants from strawberry tree （Arbutus unedo L.; AU） and dog rose （Rosa canina L.; RC）, in frankfurters elaborated with or without the addition of antioxidant additives （sodium ascorbate and nitrite） was studied. Six different types of experimental frankfurters were prepared depending on the addition of phenolic-rich extracts from RC and AU and the presence （P） or absence （C） of antioxidant additives. Thiobarbituric acid reactive substances （TBARS）-numbers signiifcantly increased during chilled storage of C-frankfurters while additives and fruit phenolics inhibited lipid oxidation in P-frankfurters. The amount of protein carbonyls signiifcantly increased in all trea.m.nts except in P-AU frankfurters. The discoloration process that occurred during the chilled storage was reduced by the addition of substances with proven antioxidant activity （P-frankfurters）. Texture characteristics as hardness, springiness, cohesiveness and gumminess also suffered a signiifcant deterioration in C-frankfurters. The use of phenolic fruit extracts in combination with traditional antioxidant additives is a successful strategy to enhance the oxidative stability of frankfurters without modifying their color and texture properties.

关键词： fruit phenolics protein oxidation lipid oxidation nitrite ascorbate

mediterranean Berries as Inhibitors of Lipid Oxidation in Porcine Burger Patties Subjected to Cooking and Chilled Storage

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Journal of Integrative Agriculture 2013年第11期12卷 1982-1992页

作者： Rui Ganho mario estévez mónica a.m.nteros David morcuende Food Science Department ESTMPolytechnic Institute of Leiria Animal Production and Food Science University of ExtremaduraAv.de la Universidad s/n Animal Production and Food Science University of ExtremaduraAv.de la Universidad s/nCáceres 10003Spain

The efifciency of extracts from Arbutus unedo L. （AU）, Crataegus monogyna L. （Cm）, Rosa canina L. （RC）, and Rubus ulmifolius Schott. （RU） to inhibit lipid oxidation in raw, cooked and cooked and chilled （2°C/12 d） porcine burger patties, was investigated. The modiifcation of the fatty acid proifle during processing trea.m.nts （cooking and chilling）, the quantitative measurements of thiobarbituric acid reactive substances （TBA-RS）, and lipid-derived volatiles, were used as indicators of lipid oxidation. Polyunsaturated fatty acids （PUFA） gradually decreased during cooking and the subsequent storage of cooked burger patties with this decrease being signiifcantly greater （P〈0.05） in control patties than in those with added berry extracts. In accordance, the control patties showed signiifcantly higher TBA-RS numbers and counts of lipid-derived volatiles in all trea.m.nts when compared to the berry-added counterparts （P〈0.05）. Results from the present work show, for the ifrst time, that extracts from A. unedo, C. monogyna, R. canina, and R. ulmifolius are promising antioxidants which could enhance the nutritional, safety and sensory properties of porcine burger patties.

关键词： berries lipid oxidation meat pattiesm TBA-RSm polyunsaturated fatty acids lipid-derived volatiles

Highly Fluorescent Dye-Doped Silica Nanoparticles Increase Flow Cytometry Sensitivity for Cancer Cell monitoring

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Nano Research 2009年第6期2卷 448-461页

作者： m.-Ca.m.n estévez meghan B.OʼDonoghue Xiaolan Chen Weihong Tan Center for Research at the Bio/Nano Interface Departments of Chemistry and Department of Physiology and Functional GenomicsShands Cancer Center and UF Genetics InstituteMcKnight Brain InstituteUniversity of FloridaGainesvilleFlorida 32611-7200USA

Early and accurate diagnosis and trea.m.nt of cancer depend on rapid,sensitive,and selective detection of tumor *** diagnosis of cancers,especially leukemia,relies on histology and fl ow cytometry using single dye-labeled ***,this combination may not lead to high signal output,which can hinder detection,especially when the probes have relatively weak affi nities or when the receptor is expressed in a low concentration on the target cell *** solve these problems,we have developed a novel method for sensitive and rapid detection of cancer cells using dye-doped silica nanoparticles(NPs)which increases detection sensitivity in fl ow cytometry analyses between 10-and 100-fold compared to standard *** NPs are~60 nm in size and can encapsulate thousands of individual dye molecules within their *** have extensively investigated surface modifi cation strategies in order to make the NPs suitable for selective detection of cancer cells using fl ow *** NPs are functionalized with polyethylene glycol(PEG)to prevent nonspecifi c interactions and with neutravidin to allow universal binding with biotinylated *** virtue of their reliable and selective detection of target cancer cells,these NPs have demonstrated their promising usefulness in conventional fl ow ***,they have shown low background signal,high signal enhancement,and effi cient functionalization,either with antibody-or aptamer-targeting moieties.

关键词： Silica nanoparticle flow cytometry aptamer cell detection polyethylene glycol fl uorophore

NLO response of derivatives of benzene, stilbene and diphenylacetylene：mP2 and DFT calculations

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Chinese Journal of Chemical Engineering 2017年第6期25卷 800-808页

作者： a.m. Elhorri m. Zouaoui-Rabah Laboratoire de microscopie Microanalyse et Spectroscopie Mol&ulaire Faculty of Sciences Djillali Liabes University of Sidi-Bel-Abbes 22000 Sidi-BeI-Abbes Algeria

mP2 method and different functionals such as m06-HF, m06-2X, CAm-B3 LYP, PBE0, B3 LYP and m06 L incorporating different XC amounts were used to calculate the static first hyperpolarizabilies β(0) of eight molecules which are derivatives of benzene, stilbene and diphenylacetylene. The values were then compared to the experimental ones. The m06-2X functional was then selected for further calculations. NBO calculations were also performed to define the intramolecular charge transfer in each molecule. TD-DFT calculations were performed taking into account the solvent effect using the IEF-PCm formalism. Some parameters characterizing the vertical transitions such as the wavelength of the maximum absorption λ_(max) were compared to the experimental ones. Finally,solvation Gibbs free enthalpy ΔG_(solv) values have also been examined in order to determine which the specific solvent for a given molecule is.

关键词： First hyperpolarizability DFT TD-DFT Push-pull molecules NBOCharge transfer

Effect of Electroplating Parameters on microstructure of Nanocrystalline Nickel Coatings

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Journal of materials Science & Technology 2010年第1期26卷 82-86页

作者： a.m. Rashidi A. Amadeh Faculty of Engineering Razi University School of metallurgy & materials Engineering University College of Engineering University of Tehran

In order to achieve the optimum conditions for electroplating nanocrystalline nickel coating from Watts-type bath, the effect of some process parameters namely, bath temperature, current density, and saccharin addition on grain size and texture coefficient （TC= I（200）/I（111）） of the deposits were investigated by X-ray diffraction （XRD）. The results showed that in a bath containing 5 g/L saccharin, by increasing the bath temperature from 45℃ to 55℃, the grain size decreased, whereas further increase of bath temperature resulted in a contrary effect. By increasing the current density from 10 to 75 mA/cm^2, both the grain size and TC decreased, while further increase in current density had no significant effect on the grain size. At a given current density, the grain size and TC decreased rapidly by increasing the saccharin content before leveling off at 3 g/L of saccharin. Finally, based on the grain refining the optimum conditions for producing nanocrystalline nickel coating from Watts-type bath have been proposed.

关键词： Electroplating Grain size Nanocrystalline Coating

Characterization and separation of pyrite from Abu Tartur black shale

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International Journal of mining Science and Technology 2015年第4期25卷 565-571页

作者： Ibrahim S.S. El Ka.m.r a.m. Guda a.m. Central metallurgical R&D Institute CMRDI Geology Dept. Faculty of Science National Research Astronomical & Geophysical Institute NRIAG

This work a.m.d for pyrite separation from Abu Tartur black shale as a source of sulfur to be an added economic value of Abu Tartur area. The considered samples in the present work were collected from a core drilled in Abu Tartur plateau representing the pyrite-rich black shale of the U. Cretaceous age, Sample characterization was carried out using petrographic microscope, XRD, DTA/DTG, C/S and XRF techniques. Clay minerals, silt-sized quartz, calcite, and hematite were the main minerals associating pyr- ite （5.34g）. Liberation behavior of the sample was about 80% below 5 μm. Sample processing was achieved through one-day soaking followed by classification using I inch mozley hydro-cyclone where about 35.5% by weight went to underflow and 64.5% went to overflow. The underflow product was subjected to an advanced gravity separation process using SB-40 Falcon Concentrator through a CCD statistical design prepared by Design-Expert 6.0 software proposed to opti- mize the separation process through a study for the effects of frequency （Hz） and water pressure （Psi） on both assay and recovery of the sulfur-rich heavy fraction. A heavy concentrate weighed 10.90% with inorganic sulfur content reached 11.37% （21.24% pyrite） with overall recovery （50.01%） was obtained after two cleaning at the optimum conditions.

关键词： Abu Tartur plateau Black shale Pyrite Falcon Concentrator

Engineering hyaluronic acid-based nanoassemblies for monoclonal antibody delivery-design,characterization,and biological insights

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Nano Research 2024年第10期17卷 9111-9125页

作者： Ana.m.López-estévez Y.Zhang maría.m.del Iker Arriaga Lucía Sanjurjo Cristian Huck-Iriart Nicola G.A.Abrescia.m.ría J.Vicent Defang Ouyang Dolores Torres maría José Alonso Center for Research in molecular medicine and Chronic Diseases(CimUS) University of Santiago de CompostelaSantiago de Compostela 15782Spain Department of Pha.m.cology Pharmacy and Pharmaceutical TechnologySchool of PharmacyUniversity of Santiago de CompostelaSantiago de Compostela 15782Spain Health Research Institute of Santiago de Compostela(IDIS) Santiago de Compostela 15782Spain State Key Laboratory of Quality Research in Chinese medicine Institute of Chinese Medical Sciences(ICMS)University of MacaoMacao 999078China Polymer Therapeutics Laboratory and CIBERONC Prince Felipe Research Centre Valencia E-46012Spain Structure and Cell Biology of Viruses Lab CIC bioGUNEBasque Research and Technology Alliance(BRTA)Derio 48160Spain ALBA Synchrotron Light Source Experiments DivisionCerdanyola del VallèsBarcelona 08290Spain IKERBASQUE Basque Foundation for ScienceBilbao 48009Spain

The current spotlight of cancer therapeutics is shifting towards personalized medicine with the widespread use of monoclonal antibodies(mAbs).Despite their increasing potential,mAbs have an intrinsic limitation related to their inability to cross cell membranes and reach intracellular *** offers promising solutions to overcome this limitation,however,formulation challenges *** challenges are the limited loading capacity(often insufficient to achieve clinical dosing),the complex formulation methods,and the insufficient characterization of mAb-loaded ***,we present a new nanocarrier consisting of hyaluronic acid-based nanoassemblies(HANAs)specifically designed to entrap mAbs with a high efficiency and an outstanding loading capacity(50%,w/w).HANAs composed by an mAb,modified HA and phosphatidylcholine(PC)resulted in sizes of~100 nm and neutral surface *** modeling identified the principal factors governing the high affinity of mAbs with the amphiphilic HA and *** composition and structural configuration were analyzed using the orthogonal techniques cryogenic transmission electron microscopy(cryo-TEm),asymmetrical flow field-flow fractionation(AF4),and small-angle X-ray scattering(SAXS).These techniques provided evidence of the formation of core-shell nanostructures comprising an aqueous core surrounded by a bilayer consisting of phospholipids and amphiphilic *** vitro experiments in cancer cell lines and macrophages confirmed HANAs’low toxicity and ability to transport mAbs to the intracellular *** reproducibility of this assembling process at industrial-scale batch sizes and the long-term stability was *** conclusion,these results underscore the suitability of HANAs technology to load and deliver biologicals,which holds promise for future clinical translation.

关键词： monoclonal antibody nanoparticles hyaluronic acid characterization particle size computational modeling

Polygeneration Study of Low-to-medium Enthalpy Geothermal Reservoirs in mexico

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Journal of Thermal Science 2021年第3期30卷 1077-1087页

作者： CHÁvez Oscar GODÍNEZ Francisco Tecnológico Nacional de méxico/I.T.Chihuahua.Av.Tecnológico 2909 C.P.31310ChihuahuaChihuahuaMéxico Instituto de Ingeniería Universidad Nacional Autónoma de MéxicoUNAMC.U.Ciudad de MéxicoC.P.04510México Polo Universitario de Tecnología Avanzada Universidad Nacional Autónoma de MéxicoVía de la Innovación No.410Autopista Monterrey-Aeropuertokm.10 PIITC.P.66629Apodaca Nuevo LeónMéxico

This study combines the thermodynamic analysis of a polygeneration system along with the numerical modelling of the thermal behavior of geothermal reservoirs in mexico to exploit their *** reservoir was modeled as a porous medium assuming a five-spot well configuration and local thermal *** heat conduction-convection along with the Laplace equations were solved to compute temperature distributions,the useful life and the optimum distance between injection-extraction *** predicted temperature and pressure of the geothermal fluid at the outlet of the reservoir were exploited in the polygeneration system consisting of:(1)a Rankine cycle,(2)an absorption refrigeration cycle,and(3)a heat *** developed approach allows calculating both the optimal distance between injection-extraction wells and the global(utilization)efficiency of six arrangements(each composed by a reservoir connected to a polygeneration system)by assuming that reservoirs have a lifespan of 30 *** also show that:(a)due to the low efficiency of the Rankine cycle,very little thermal energy is converted into electrical one;(b)not only the temperature and the size are important when evaluating the power production performance of reservoirs,but also the permeability plays a fundamental role;(c)the first law efficiency of the polygeneration system ranges from 41.9%to 43.7%;(d)the utilization efficiency of the six arrangements lies in the range between 25.8%and 31%.

关键词： polygeneration system geothermal reservoir local thermal equilibrium low-to-medium enthalpy

Effective Lower Bounds of Wrap-Around L_2-Discrepancy on Three-Level Combined Designs

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Journal of Systems Science & Complexity 2017年第6期30卷 1459-1469页

作者： ELSAWAH a.m. HU Jianwei QIN Hong Faculty of mathematics and Statistics Central China Normal University Division of Science and Technology BNU-HKBU United International College Department ofmathematics Faculty of Science Zagazig University

How to obtain an effective design is a.m.jor concern of scientific research. This topic always involves high-dimensional inputs with limited resources. The foldover is a quick and useful technique in construction of fractional designs, which typically releases aliased factors or *** paper takes the wrap-around L_2-discrepancy as the optimality measure to assess the optimal three-level combined designs. New and efficient analytical expressions and lower bounds of the wraparound L_2-discrepancy for three-level combined designs are obtained. The new lower bound is useful and sharper than the existing lower bound. Using the new analytical expression and lower bound as the benchmarks, the authors may implement an effective algorithm for constructing optimal three-level combined designs.

关键词： Combined design Foldover plan L2-discrepancy lower bound optimal combined design wrap-around