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p53 promotes AKT and SP1-dependent metabolism through the pentose phosphate pathway that inhibits apoptosis in response to Nutlin-3a

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Journal of Molecular Cell Biology 2018年第4期10卷 331-340页

作者： Text Lei Duan Ricardo e. Perez Ling Chen Lothar A. Blatter Carl G. Maki Department of Cell & Molecular Medicine Rush University Medical Center Chicago IL 60612 USA Department of Laboratory Medicine Shiyan Taihe Hospital College of Biomedical Engineering Hubei University of Medicine Shiyan 442000 China Department of Physiology & Biophysics Rush University Medical Center Chicago IL 60612 USA

Nutlin-3a is a MDM2 antagonist and preclinical drug that activates p53. Cells with MDM2 gene amplification are especially prone to Nutlin-3a-induced apoptosis, though the basis for this is unclear. Glucose metabolism can inhibit apoptosis in response to Nutlin-3a through mechanisms that are incompletely understood. Glucose metabolism through the pentose phosphate pathway （PPP） produces NADPH that can protect cells from potentially lethal reactive oxygen species （ROS）. We compared apoptosis and glucose metabolism in cancer cells with and without MDM2 gene amplification treated with Nutlin-3a. Apoptosis in MDM2-amplified cells was associated with a reduction in glycolysis and the PPP, reduced NADPH, increased ROS, and depletion of the transcription factor SP1, which normally promotes PPP gene expression. In contrast, glycolysis and the PPP were maintained or increased in MDM2 non-amplified cells treated with Nutlin-3a. This was dependent on p53-mediated AKT activation and was associated with maintenance of SP1 and continued expression of PPP genes. Knockdown or inhibition of AKT, SP1, or the PPP sensitized MDM2-non-amplified cells to apoptosis. The data indicate that p53 promotes AKT and SP1-dependent activation of the PPP that protects cells from Nutlin-3a-induced apoptosis. These findings provide insight into how glucose metabolism reduces Nutlin-3a-induced apoptosis, and also provide a mechanism for the heightened sensitivity of MDM2-amplified cells to apoptosis in response to Nutlin-3a.

关键词： Nutlin p53 MDM2 SP1 glycolysis pentose phosphate pathway

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Generic sensor platform based on electro- responsive molecularly imprinted polymer nanoparticles (e-NanoMIPs)

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Microsystems & Nanoengineering 2020年第1期6卷 266-274页

作者： A.Garcia-Cruz O.S.Ahmad K.Alanazi e.Piletska S.A.Piletsky School of Chemistry University of LeicesterLeicesterUK

The present research describes the design of robust electrochemical sensors based on electro-responsive molecularly imprinted polymer nanoparticles(e-MIPs).The e-MIPs,tagged with a redox probe,combine both recognition and reporting *** system replaces enzyme-mediator pairs used in traditional *** analyte recognition process relies on the generic actuation phenomenon when the polymer conformation of e-MIPs is changing in response to the presence of the template *** analyte concentration is measured using voltammetric *** an exemplification of this technology,electrochemical sensors were developed for the determination of concentrations of trypsin,glucose,paracetamol,C4-homoserine lactone,and *** present technology allows for the possibility of producing generic,inexpensive,and robust disposable sensors for clinical,environmental,and forensic applications.

关键词： functions. methods. polymer

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Optimizing methyl-eugenol aromatherapy to maximize posttreatment effects to enhance mating competitiveness of male Bactrocera carambolae （Diptera： Tephritidae）

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Insect Science 2015年第5期22卷 661-669页

作者： Ihsan ul Haq Marc J.B. Vreysen Carlos Caceres Todd e. Shelly Jorge Hendrichs Insect Pest Control Laboratory Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Seibersdorf Austria National Agricultural Research Centre Park Road Islamabad Pakistan USDAIAPHIS 41-650 Ahiki Street Waimanalo HI 96795 USA and Insect Pest Control Section Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Vienna Austria

Methyl-eugenol （Me）（1,2-dimethoxy-4-（2-propenyl）benzene）, a natural phy- tochemical, did enhance male Bactrocera carambolae Drew ＆ Hancock （Diptera： Tephri- tidae） mating competitiveness 3 d after ingestion. enhanced male mating competitiveness can significantly increase the effectiveness of the sterile insect technique （SIT）. Me appli- cation to mass reared sterile flies by feeding is infeasible. Me application by aromatherapy however, would be a very practical way of Me application in fly emergence and release facilities. This approach was shown to enhance mating competitiveness ofB. carambolae 3 d posttreatment （DPT）. Despite this added benefit, every additional day of delaying re- lease will reduce sterile fly quality and will add cost to SIT application. The present study was planned to assess the effects of Me-aromatherapy on male B. carambolae mating competitiveness 1DPT and 2DPT. Me aromatherapy 1DPT or 2DPT did enhance mating competitiveness of B. carambolae males whereas Me feeding 1DPT and 2DPT did not. Male mating competitiveness was enhanced by the Me aromatherapy irrespective if they received 1DPT, 2DPT or 3DPT. Me aromatherapy, being a viable approach for its applica- tion, did enhance mating competitiveness of male B. carambolae 1 d posttreatrnent as Me feeding did 3 d after ingestion.

关键词： Bactrocera carambolae mating competitiveness Me aromatherapy methyl-eugenol Me feeding posttreatment effect SIT

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experimental Study on Heterograft of Glomus Cells of Carotid Body for Hemiparkinsonian Rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第2期22卷 129-131页

作者： CAO Xuebing(曹学兵) SUN Shenggang(孙圣刚) TONG e＇tang(童萼塘) Department of Neurology Xiehe Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430022 Department of Neurology Xiehe Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430022 Department of Neurology Xiehe Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430022

Summary: To observe the effects of heterograft of glomus cells of carotid body on hemiparkinsonian rat models, rats with unilateral 6-hydroxydopamine (6-OHDA)-induced lesions of the right dopaminergic neurons of substantia nigra received intrastriatal glomus cells heterograft. Apomorphine-induced rotation was monitored for 30 min at various time points after grafting. The striata were cut and examined for dopamine content by HPLC and for immunohistochemical staining of tyrosine hydroxylase positive neurons (TH +) at the end of the experiments. The results showed that apomorphine-induced rotational behavior was significantly reduced for 12 weeks and the dopamine contents were significantly elevated after grafting (Pells survived better. The present study demonstrates that intrastriatal heterograft of glomus cells within carotid body in rats with 6-OHDA-elicited lesions could reduce apomorphine-induced rotational behavior and elevate the dopamine contents and numbers of TH + cell surviving within striatum, and can serve as a new and effective alternative for Parkinson disease.

关键词： carotid body glomus cell heterograft Parkinson disease

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Mass transfer and loss of the massive semi-detached binary AI Crucis

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Research in Astronomy and Astrophysics 2010年第5期10卷 438-444页

作者： er-Gang Zhao Sheng-Bang Qian e. Femanindez Lajus Carolina von essen Li-Ying Zhu National Astronomical Observatories / Yunnan Observatory Chinese Academy of SciencesKunming 650011 China Key Laboratory for the Structure and evolution of Celestial Objects Chinese Academy ofSciences Kunming 650011 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Facultad de Ciencias Astron6micas y Geoffsicas Universidad Nacional de La Plata 1900 LaPlata Buenos Aires Argentina

AI Crucis is a short-period semi-detached massive close binary （P = 1.41771d, Sp.=B1.5） in the open cluster NGC 4103. It is a good astrophysical laboratory for investigating the formation and evolution of massive close binary stars via case A mass transfer. Orbital period variations of the system were analyzed based on one newly determined eclipse time and the others compiled from the literature. It is discovered that the orbital period of the binary is continuously increasing at a rate of dP/dt = ＋1.00（±0.04） × 10-7 d yr-1. After the long-term increase is subtracted from the O - C diagram, weak evidence indicates the presence of a cyclic oscillation with a period of 30.1yr, which may reveal a very cool stellar companion in the system. The long-term period increase can be explained by mass transfer from the less massive component to the more massive one. This is in agreement with the semidetached configuration of the binary, indicating that the system is undergoing a slow mass-transfer stage on the nuclear time scale of the secondary. However, it is found that the slow mass transfer is insufficient to cause the observed period increase, which suggests that the stellar wind from the hot component should contribute to the amount of period increase dP/dt = ＋0.54× 10-7 d yr-1 that corresponds to a mass loss rate of M˙1 = 2.72 × 10-7 M yr-1. It is estimated that the hot component lost a total mass of 4.1M during the slow mass-transfer stage and, thus, the evolution of the binary system should be changed greatly by the mass loss.

关键词： stars: binaries: close — stars: binaries: eclipsing — stars: individual (AI Crucis) — stars: early-type — stars: evolution

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Cdc42 regulates the cellular localization of Cdc42ep1 in controlling neural crest cell migration

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Journal of Molecular Cell Biology 2018年第5期10卷 376-387页

作者： Shlomi Cohen Daniel T. Kovari Wenbin Wei Rebecca Keate Jennifer e. Curtis Shuyi Nie School of Biological Sciences Georgia institute of Technology Atlanta GA 30332 USA School of Physics Georgia Institute of Technology Atlanta GA 30332 USA Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology Atlanta GA 30332 USA Department of Physics Emory University Atlanta GA 30322 USA Wallace H. Coulter Department of Biomedical engineering Georgia institute of Technology Atlanta GA 30332 USA

The member of Rho family of small GTPases Cdc42 plays important and conserved roles in cell polarity and motility. The Cdc42ep family proteins have been identified to bind to Cdc42, yet how they interact with Cdc42 to regulate cell migration remains to be elucidated. In this study, we focus on Cdc42epl, which is expressed predominantly in the highly migratory neural crest ceils in frog embryos. Through morpholino-mediated knockdown, we show that Cdc42epl is required for the migration of cranial neural crest cells. Loss of Cdc42epl leads to rounder cell shapes and the formation of membrane blebs, consistent with the observed disruption in actin organization and focal adhesion alignment. As a result, Cdc42ep1 is critical for neural crest cells to apply traction forces at the correct place to migrate efficiently. We further show that Cdc42ep1 is localized to two areas in neural crest celts： in membrane protrusions together with Cdc42 and in perinuciear patches where Cdc42 is absent. Cdc42 directly interacts with Cdc42epl （through the CRIB domain） and changes in Cdc42 level shift the distribution of Cdc42epl between these two subcellular locations, controlling the formation of membrane protrusions and directionality of migration as a consequence. These results suggest that Cdc42ep1 elaborates Cdc42 activity in neural crest cells to promote their efficient migration.

关键词： actin cytoskeleton neural crest Rho GTPases Cdc42 effector protein 1 cell migration

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Short-pulse laser-driven x-ray radiography

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High Power Laser Science and engineering 2016年第3期4卷 101-105页

作者： e.Brambrink S.Baton M.Koenig R.Yurchak N.Bidaut B.Albertazzi J.e.Cross G.Gregori A.Rigby e.Falize A.Pelka F.Kroll S.Pikuz Y.Sakawa N.Ozaki C.Kuranz M.Manuel C.Li P.Tzeferacos D.Lamb LULI-CNRS Ecole PolytechniqueCEA Universit Paris-Saclay UPMC Univ Paris 06 Sorbonne Universits Institute for Academic Initiatives Osaka U. Clarendon Laboratory University of Oxford CeA-DAM-DIF HZDR JIHT-RAS Institute of Laser engineering Osaka U. Graduate School of engineering Osaka U. Department of energy engineering Science Faculty of Engineering SciencesKyushu University Plasma Science and Fusion Center Massachusetts Institute of Technology Flash Center for Computational Science University of Chicago

We have developed a new radiography setup with a short-pulse laser-driven x-ray source. Using a radiography axis perpendicular to both long- and short-pulse lasers allowed optimizing the incident angle of the short-pulse laser on the x-ray source target. The setup has been tested with various x-ray source target materials and different laser *** to noise ratios are presented as well as achieved spatial resolutions. The high quality of our technique is illustrated on a plasma flow radiograph obtained during a laboratory astrophysics experiment on POLARs.

关键词： laboratory astrophysics short-pulse laser x-ray radiography

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Vessels Segmentation in Angiograms Using Convolutional Neural Network: A Deep Learning Based Approach

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Computer Modeling in engineering & Sciences 2023年第7期136卷 241-255页

作者： Sanjiban Sekhar Roy Ching-Hsien Hsu Akash Samaran Ranjan Goyal Arindam Pande Valentina e.Balas School of Computer Science and engineering Vellore Institute of TechnologyVellore632014India Department of Computer Science and Information engineering Asia UniversityTaichung41354Taiwan Department of Medical Research China Medical University HospitalChina Medical UniversityTaichung406Taiwan Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology School of Mathematics and Big DataFoshan UniversityFoshan528000China Medica Superspecialty Hospital Kolkata700099India Automation and Applied Informatics Aurel Vlaicu University of AradArad310130Romania

Coronary arterydisease(CAD)has become a significant causeof heart attack,especially amongthose 40yearsoldor *** is a need to develop new technologies andmethods to deal with this *** researchers have proposed image processing-based solutions for CADdiagnosis,but achieving highly accurate results for angiogram segmentation is still a *** different types of angiograms are adopted for CAD *** paper proposes an approach for image segmentation using ConvolutionNeuralNetworks(CNN)for diagnosing coronary artery disease to achieve state-of-the-art *** have collected the 2D X-ray images from the hospital,and the proposed model has been applied to *** augmentation has been performed in this research as it’s the most significant task required to be initiated to increase the dataset’s ***,the images have been enhanced using noise removal techniques before being fed to the CNN model for segmentation to achieve high *** the output,different settings of the network architecture undoubtedly have achieved different accuracy,among which the highest accuracy of the model is 97.61%.Compared with the other models,these results have proven to be superior to this proposed method in achieving state-of-the-art results.

关键词： Angiogram convolution neural network coronary artery disease diagnosis of CAD image segmentation

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迷走神经刺激的脑-肠轴机制研究现状

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癫痫杂志 2024年第5期10卷 411-416页

作者：曾龙刘浩霖谢韬袁金娥徐玉婷南方医科大学珠江医院神经外科中心广州510000 脑血管病诊断与治疗教育部工程研究中心广州510000 广东省普通高校脑功能修复与再生重点实验室广州510000 广东神经外科研究所广州510000

迷走神经刺激最早用于治疗难治性癫痫和抑郁症,近年来其适应证不断扩大。脑-肠轴是连接于肠道与大脑的双向通讯网络通路,维持着肠道微生物的稳态平衡并塑造大脑功能。迷走神经在神经系统疾病的脑-肠轴机制中扮演着重要的角色,这可能是... 详细信息

迷走神经刺激最早用于治疗难治性癫痫和抑郁症,近年来其适应证不断扩大。脑-肠轴是连接于肠道与大脑的双向通讯网络通路,维持着肠道微生物的稳态平衡并塑造大脑功能。迷走神经在神经系统疾病的脑-肠轴机制中扮演着重要的角色,这可能是迷走神经刺激治疗相关疾病的重要依据。最近的研究表明迷走神经刺激能调节肠道微环境及肠道菌群,但是这种改变的具体机制还需要进一步研究。粪菌移植或口服益生菌联合迷走神经刺激在未来可能成为重要的治疗手段,尤其是提高迷走神经刺激对癫痫的疗效;肠道菌群也可能成为迷走神经刺激治疗癫痫疗效的预测靶点。

关键词：迷走神经刺激脑-肠轴癫痫肠道菌群

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Growth and Cd Accumulation of Orychophragmus violaceus as Affected by Inoculation of Cd-Tolerant Bacterial Strains

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Pedosphere 2014年第3期24卷 322-329页

作者： LIANG Xia He Chi-Quan NI Gang TANG Gui-e CHeN Xue-Ping LeI Yan-Ru School of environmental and Chemical engineering Shanghai University

Beneficial interactions between microorganisms and plants, particularly in the rhizosphere, are a research area of global interest. Four cadmium(Cd)-tolerant bacterial strains were isolated from heavy metal-contaminated sludge and their effects on Cd mobility in soil and the root elongation and Cd accumulation of Orychophragmus violaceus were explored to identify the capability of metalresistant rhizobacteria for promoting the growth of O. violaceus roots on Cd-contaminated soils. The isolated strains, namely, Bacillus subtilis, B. cereus, B. megaterium, and Pseudomonas aeruginosa, significantly enhanced the plant Cd accumulation. The Cd concentrations in the roots and shoots were increased by up to 2.29- and 2.86-fold, respectively, by inoculation of B. megaterium, as compared with the uninoculated control. The bacterial strains displayed different effects on the shoot biomass. Compared with the uninoculated plants, the shoot biomass of the inoculated plants was slightly increased by B. megaterium and significantly decreased by the other strains. B. megaterium was identified as the best candidate for enhancing Cd accumulation in O. violaceus. Thus, this study provides novel insight into the development of plant-microbe systems for phytoremediation.

关键词： heavy metals phytoremediation plant-microbe systems rhizobacteria soil contamination

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