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Dendritic cell-mimicking scaffolds for ex vivo T cell expansion

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Bioactive Materials 2023年第3期21卷 241-252页

作者： Hye Sung Kim Tzu-Chieh Ho Moshe J.Willner Michael W.becker Hae-Won Kim Kam W.Leong Department of Biomedical Engineering Columbia UniversityNew YorkNYUSA Institute of Tissue Regeneration Engineering Dankook UniversityCheonanRepublic of Korea Wilmot Cancer Institute University of Rochester Medical CenterRochesterNYUSA Department of Regenerative Dental Medicine College of DentistryDankook UniversityCheonanRepublic of Korea Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonanRepublic of Korea Mechanobiology Dental Medicine Research Center Dankook UniversityCheonanRepublic of Korea Department of Systems Biology Columbia University Irving Medical CenterNew YorkNY10032USA

We propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells(APCs)for adoptive cell therapy(ACT).Microfiber scaffolds coated with dendritic cell(DC)membrane replicate physicochemical properties of dendritic cells specific for T cell activation such as rapid recognition by T cells,long duration of T cell tethering,and DC-specific co-stimulatory *** DC membrane-coated scaffold is first surface-immobilized with T cell stimulatory ligands,anti-CD3(αCD3)and anti-CD28(αCD28)antibodies,followed by adsorption of releasable interleukin-2(IL-2).The scaffolds present both surface and soluble cues to T cells ex vivo in the same way that these cues are presented by natural APCs in *** demonstrate that the DC-mimicking scaffold promotes greater polyclonal expansion of primary human T cells as compared toαCD3/αCD28-func-tionalized *** importantly,major histocompatibility complex molecules derived from the DC membrane of the scaffold allow antigen-specific T cell expansion with target cell-specific killing *** addition,most of the expanded T cells(~97%)can be harvested from the scaffold by density gradient ***,the DC-mimicking scaffold offers a scalable,modular,and customizable platform for rapid expansion of highly functional T cells for ACT.

关键词： Adoptive cell therapy Ex vivo T cell expansion Cytotoxic T cell Dendritic cell membrane Cell membrane coating Scaffold

A Transcriptome Atlas of Physcomitrella patens Provides Insights into the Evolution and Development of Land Plants

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Molecular Plant 2016年第2期9卷 205-220页

作者： Carlos Ortiz-Ramirez Marcela Hernandez-Coronado Anna Thamm Bruno Catarino Mingyi Wang Liam Dolan Jose A.Feijo Jorg D. becker Instituto Gulbenklan de Ciencia Rua da Quinta Grande 6 2780-156 Oeiras Portugal Department of Plant Sciences University of Oxford South Parks Road Oxford OX1 3RB UK Division of Plant Biology The Samuel Roberts Noble Foundation Ardmore OK 73401 USA Department of Cell Biology and Molecular Genetics University of Maryland 0118 BioSclence Research Building College Park MD 20742-5815 USA These authors contributed equally to this article

Identifying the genetic mechanisms that underpin the evolution of new organ and tissue systems is an aim of evolutionary developmental biology. Comparative functional genetic studies between angiosperms and bryophytes can define those genetic changes that were responsible for developmental innovations. Here, we report the generation of a transcriptome atlas covering most phases in the life cycle of the model bryo- phyte Physcomitrella patens, including detailed sporophyte developmental progression. We identified a comprehensive set of sporophyte-specific transcription factors, and found that many of these genes have homologs in angiosperms that function in developmental processes such as flowering and shoot branching. Deletion of the PpTCP5 transcription factor results in development of supernumerary sporangia attached to a single seta, suggesting that it negatively regulates branching in the moss sporophyte. Given that TCP genes repress branching in angiosperms, we suggest that this activity is ancient. Finally, compar- ison of P. patens and Arabidopsis thaliana transcriptomes led us to the identification of a conserved core of transcription factors expressed in tip-growing cells. We identified modifications in the expression patterns of these genes that could account for developmental differences between P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.

关键词： land plant evolution Physcomitrella patens transcriptome atlas TCP branching tip growth

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验证LTE基带接收机

电子技术应用 2009年第10期35卷 4-5页

作者： Randy becker 安捷伦科技公司

在传统意义上的无线通信系统中，射频领域的模拟信号可使用模拟技术解调为同相（I）和正交（Q）分量。但在当今软件定义的无线技术时代，中频信号经过变频处理后通常会使用模数转换器（ADC）进行数字化处理，然后再将数字信号馈入基带... 详细信息

在传统意义上的无线通信系统中，射频领域的模拟信号可使用模拟技术解调为同相（I）和正交（Q）分量。但在当今软件定义的无线技术时代，中频信号经过变频处理后通常会使用模数转换器（ADC）进行数字化处理，然后再将数字信号馈入基带进行解调和解码。现在，通过将信号发生器的输出频率设为目标中频来生成恰当的中频（IF）信号，可以在射频领域以外测试基带接收机。

关键词：接收机基带 LTE 验证无线通信系统中频信号模拟信号数字化处理

Positive predictors for gastroesophageal reflux disease and the therapeutic response to proton-pump inhibitors

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World Journal of Gastroenterology 2014年第14期20卷 4017-4024页

作者： Valentin becker Stefan Grotz Christoph Schlag Simon Nennstiel Analena Beitz Bernhard Haller Roland M Schmid Alexander Meining Monther Bajbouj Ⅱ. Medizinische Klinik und Poliklinik Technische Universit?t München Institut für Medizinische Statistik und Epidemiologie Technische Universit?t München

AIM: To identify objective and subjective predictors for the reliable diagnosis of gastroesophageal reflux disease (GERD) and the response to proton pump inhibitor (PPI) therapy.

关键词： Gastroesophageal reflux Gastroesophageal reflux disease Non-erosive reflux disease Impedance pH measurement Follow-up Therapy Proton pump inhibitor

In-Game Ads Put Brands in Play——How disappearing demographics can be recovered through their video game consoles

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成功营销 2008年第8期 46-48页

作者： Grant Jenman Stephanie becker

Your audience will continue to ***'s that ***'ve already lost young men to digital *** demographics disappear from traditional media every *** the problem gets worse:Married women are spending more time playing online... 详细信息

关键词：营销策略广告企业管理品牌

Marker Based Genetic Analysis For Yield Components And Their Relationships With Developmental Stages In A European x Chinese Rapeseed DH population

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Marker Based Genetic Analysis For Yield Components And Their...

中国遗传学会植物遗传与基因组学专业委员会2005年学术研讨会

作者： becker Heiko ECKE Wolfgang Institute of Agronomy and Plant Breeding Georg-August-UniversityVon Siebold Strasse 837075 GoettingenGermany

In this study, we mapped QTLs with additive and additive x additive epistasis as well as their interactions by environments underlying three quantitative traits of yield components using a population of 282 doubled haploid lines derived from a cross between European and Chinese rapeseed cultivars. Furthermore, we did conditional mapping analysis by combining the statistical procedures of analyzing conditional genetic effects with QTL mapping method to analyze the genetic relationship between yield components and plant developmental stages on individual QTL level. Measurements for the traits were obtained from 4 environments, 2 in Europe and 2 in China. A linkage map was constructed using 125 microsatellites marker loci. Mapping results revealed in total 7, 6 and 9 QTLs with additive main effects and/or additive by environment interaction effects for these three traits and collectively explained about 40, 35 and 70% of the mean phenotypic variation observed in the DH population, respectively. Digenic epistasis was small for thousand seeds weight, negligible for silique length but significant for the *** seeds per silique. Significant interaction was detected for thousand seeds weight, while minor for other two traits. The genetic relationships among these three traits were discussed on the QTL level. Information obtained from this study is valuable for marker-based selection to improve yield components and yield level. in breeding program. Conditional mapping analysis revealed that QTLs for thousand seeds weight were more related with flowering time and grain filling period, while QTLs for the number of seeds per silique were more influenced by flowering period.

关键词： Brassica napus L. Yield components QTL mapping Conditional QTL mapping Digenic interaction QTL by environment interaction

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Salt Stress Triggers Phosphorylation of the Arabidopsis Vacuolar K＋ Channel TPK1 by Calcium-Dependent Protein Kinases （CDPKs）

作者： becker等

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Molecular Plant 2013年第4期6卷 1274-1289页

作者： Andreas Latz Norbert Mehlmer Simone Zapf Thomas D. Mueller Bernhard Wurzinger Barbara Pfister Edina Csaszar Rainer Hedrich Markus Teige Dirk becker Department of Molecular Plant Physiology and Biophysics Julius-von-Sachs-lnstitute University of Wurzburg Julius-von-Sachs-Platz 2 D-97082 Wurzburg Germany Department of Biochemistry and Cell Biology Max F. Perutz Laboratories University of Vienna Dr. Bohrgasse 9 A-1030 Vienna Austria Present address: Department of Biology I Botany Munich Center for Integrated Protein Science CiPSM Ludwig-Maximilians-Universitat Munchen D-82152 Planegg-Martinsried Germany College of Science King Saud University Riyadh 11451 Saudi Arabia

14-3-3 proteins play an important role in the regulation of many cellular processes. The Arabidopsis vacuolar two-pore K＋ channel 1 （TPK1） interacts with the 14-3-3 protein GRF6 （GF14-λ）. Upon phosphorylation of the putative binding motif in the N-terminus of TPK1, GRF6 binds to TPK1 and activates the potassium channel. In order to gain a deeper understanding of this 14-3-3-mediated signal transduction, we set out to identify the respective kinases, which regulate the phosphorylation status of the 14-3-3 binding motif in TPK1. Here, we report that the calcium-dependent protein kinases （CDPKs） can phosphorylate and thereby activate the 14-3-3 binding motif in TPK1. Focusing on the stress-activated kinase CPK3, we visualized direct and specific interaction of TPK1 with the kinase at the tonoplast in vivo. In line with its proposed role in K＋ homeostasis, TPK1 phosphorylation was found to be induced by salt stress in planta, and both cpk3 and tpkl mutants displayed salt-sensitive phenotypes. Molecular modeling of the TPK1-CPK3 interaction domain provided mechanistic insights into TPK1 stress-regulated phosphorylation responses and pinpointed two arginine residues in the N-terminal 14-3-3 binding motif in TPK1 critical for kinase interaction. Taken together, our studies provide evidence for an essential role of the vacuolar potassium channel TPK1 in salt-stress adaptation as a target of calcium-regulated stress signaling pathways involving Ca2＋, Ca2＋-dependent kinases, and 14-3-3 proteins.

关键词： potassium channel vacuole calcium calcium-dependent kinase 14-3-3 protein salt stress.

Towards an integrated modeling of the plasma-solid interface

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Frontiers of Chemical Science and Engineering 2019年第2期13卷 201-237页

作者： Michael Bonitz Alexey Filinov Jan-Willem Abraham Karsten Balzer Hanno Kahlert Eckhard Pehlke Franz X.Bronold Matthias Pamperin Markus becker Dettlef Loffhagen Holger Fehske Institute for Theoretical Physics and Astrophysics Kiel University24098 KielGermany Leibniz Institute for Plasma Research (INP) D-17489 GreifswaldGermany Joint Institute for High Temperatures RAS 125412 MoscowRussia Computing Center of Kiel University D-24118 KielGermany Institute of Physics Greifswald UniversityD-17489 GreifswaldGermany

Solids facing a plasma are a common situation in many astrophysical systems and laboratory ***,many plasma technology applications rely on the control of the plasma-surface interaction,i.e.,of the particle,momentum and energy fluxes across the plasma-solid ***,presently often a fundamental understanding of them is missing,so most technological applications are being developed via trial and *** reason is that the physical processes at the interface of a low-temperature plasma and a solid are extremely complex,involving a large number of elementary processes in the plasma,in the solid as well as fluxes across the *** accurate theoretical treatment of these processes is very difficult due to the vastly different system properties on both sides of the interface:Quantum versus classical behavior of electrons in the solid and plasma,respectively;as well as the dramatically differing electron densities,length and time ***,often the system is far from *** the majority of plasma simulations surface processes are either neglected or treated via phenomenological parameters such as sticking coefficients,sputter rates or secondary electron emission ***,those parameters are known only in some cases and with very limited ***,while surface physics simulations have often studied the impact of single ions or neutrals,so far,the influence of a plasma medium and correlations between successive impacts have not been taken into *** an approach,necessarily neglects the mutual influences between plasma and solid surface and cannot have predictive *** this paper we discuss in some detail the physical processes of the plasma-solid interface which brings us to the necessity of coupled plasma-solid *** briefly summarize relevant theoretical methods from solid state and surface physics that are suitable to contribute to such an approach and identify four *** first are

关键词： plasma physics surface science plasmasurface modeling DFT nonequilibrium Green functions