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Molecular and physiological characterization of the effects of auxin-enriched rootstock on grafting

Horticulture Research 2021年第1期8卷 926-938页

作者： Longmei Zhai Xiaomin Wang Dan Tang Qi Qi Huseyin yer Xiangning Jiang Zhenhai Han Richard McAvoy Wei Li Yi Li Department of Plant Science and Landscape Architecture University of ConnecticutStorrsCT 06269USA College of Horticulture China Agricultural UniversityBeijing 100193PR China Institute of botany Jiangsu Province and Chinese Academy of SciencesNanjing 210014PR China National Engineering Laboratory for Tree breeding College of Life Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083PR China

Grafting is a highly useful technique,and its success largely depends on graft union *** this study,we found that root-specific expression of the auxin biosynthetic gene iaaM in tobacco,when used as rootstock,resulted in more rapid callus formation and faster graft ***,overexpression of the auxin-inactivating iaaL gene in rootstocks delayed graft *** observed increased endogenous auxin levels and auxin-responsive DR5::GUS expression in scions of WT/iaaM grafts compared with those found in WT/WT grafts,which suggested that auxin is transported upward from rootstock to scion tissues.A transcriptome analysis showed that auxin enhanced graft union formation through increases in the expression of genes involved in graft healing in both rootstock and scion *** also observed that the ethylene biosynthetic gene ACS1 and the ethylene-responsive gene ERF5 were upregulated in both scions and rootstocks of the WT/iaaM ***,exogenous applications of the ethylene precursor ACC to the junction of WT/WT grafts promoted graft union formation,whereas application of the ethylene biosynthesis inhibitor AVG delayed graft healing in WT/WT grafts,and the observed delay was less pronounced in the WT/iaaM *** results demonstrated that elevated auxin levels in the iaaM rootstock in combination with the increased auxin levels in scions caused by upward transport/diffusion enhanced graft union formation and that ethylene was partially responsible for the effects of auxin on *** findings showed that grafting success can be enhanced by increasing the auxin levels in rootstocks using transgenic or gene-editing techniques.

关键词： union enriched characterization

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