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Investigating the genetic diversity of several varieties of Iranian tomato (Solanum lycopersicum) using RAPD and ISSR molecular markers

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Food and Health 2024年第4期6卷 19-26页

作者： Bahareh Ahang sanaz Pashapour Aida Ghasemi Abbas Zabihi Department of Biology Faculty of Basic SciencesIslamic Azad UniversityRashtBranchRasht 0098813Iran Department of Pharmacology and toxicology Faculty of Pharmacy and Pharmaceutical SciencesIslamic Azad UniversityMedical Sciences BranchTehranIran School of Medicine Medical University of HamedanHamedanIran

Background:Numerous studies have demonstrated the existence of approximately 7,500 genetic tomato varieties ***,it is crucial to assess the genetic diversity among tomato *** study aimed to investigate the genetic diversity of selected Iranian tomato cultivars(Solanum lycopersicum)using RAPD and ISSR molecular ***:Ten RAPD primers and ten ISSR primers were employed to assess the genetic diversity among 10 tomato cultivars:Matin,RFT 112,Hirad,Golsar,Raha,Hengam,Hedah,Fasa,JS12,and *** analysis involved the UPGMA algorithm and NTYSYSpc ***:RAPD analysis revealed close genetic proximity between Fasa and JS12,as well as between Raha and ***,the RFT 112,Hengam,Hirad,and Emerald cultivars exhibited significant genetic diversity within this *** primer analysis identified Hengam as the most diverse variety,while Matin,Emerald,and Vibrid,as well as Raha and JS12,displayed genetic similarities with minimal observed ***,the overall analysis of the cultivars using RAPD and ISSR markers indicated that Hengam exhibited the highest diversity among all the ***,Raha and JS12 demonstrated limited diversity in this ***:This research demonstrates substantial genetic diversity among the investigated tomato varieties,with Hengam displaying the highest diversity within this ***,ISSR markers proved more effective in determining genetic diversity in tomato plants.

关键词： Solanum lycopersicum tomato genetic diversity RAPD ISSR

Duphaston as a suitable medication for women taking intracytoplasmic sperm injection to treat infertility

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Reproductive and Developmental Medicine 2024年第2期8卷 89-92页

作者： Leila Naserpour Katayon Berjis sanaz Soleymani Moud Rahil Jannatifar Department of Reproductive Biology Academic Center for EducationCultureand Research(ACECR)Qom BranchQomIran Infertility Treatment Center Academic Center for EducationCultureand Research(ACECR)Qom BranchQomIran Cellular and Molecular Research Center Qom University of Medical SciencesQomIran Midwifery Ward Infertility Treatment CenterAcademic Center for EducationCultureand Research(ACECR)Qom BranchQomIran

Objective:Duphaston is used as an alternative to gonadotropin-releasing hormone(GnRH)antagonists for suppressing premature luteinizing hormone(LH)*** study aimed to compare the efficacy of Duphaston protocols with that of Cetrotide in suppressing the LH ***:In this retrospective cross-sectional study,669 infertile women(aged 25-40 years)were administered recombinant follicle-stimulating hormone(rFSH)from the third day of their menstrual *** the follicular diameter reached 13 to 14 mm,Cetrotide was prescribed orally to the control subjects,also case subjects took Duphaston *** retrieved oocytes were fertilized by an intracytoplasmic sperm injection(ICSI).Results:The levels of FSH,LH,and estradiol did not differ significantly between the groups(P>0.05).However,the findings indicated that Duphaston led to greater oocyte retrieval than Cetrotide(P=0.006).However,the number of mature oocytes and viable embryos in the case subjects did not reach a significant difference compared to that in the control subjects(P>0.05).The number of good-quality embryos(stage 1)was significantly higher in the case subjects(P=0.04).In addition,there is no significant difference in the chemical,clinical pregnancy,implantation rate,and miscarriage rate between both groups(P>0.05).Conclusion:Duphaston could be utilized as a suitable medication rather than a GnRH antagonist in women subjected to controlled ovarian hyperstimulation.

关键词： Duphaston Cetrotide Progesterone protocol Ovarian hyperstimulation GnRH antagonist

Protective effects of cyclosporine and its analog NIM-811 in a murine model of hepatic ischemia-reperfusion injury

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Liver Research 2024年第1期8卷 46-53页

作者： Joshua Hefler Rena Pawlick Braulio A.Marfil-Garza Aducio Thiesen Nerea Cuesta-Gomez sanaz Hatami Darren H.Freed Constantine Karvellas David L.Bigam A.M.James Shapiro Department of Surgery Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada Department of Medicine Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada CHRISTUS-LatAm Hub Excellence&Innovation Center MonterreyMexico Alberta Diabetes Institute University of AlbertaEdmontonABCanada Clinical Islet Transplant Program University of AlbertaEdmontonABCanada Department of Laboratory Medicine&Pathology Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada

Background and aim:The liver is susceptible to ischemia-reperfusion injury(IRI)during hepatic surgery,when the vessels are compressed to control bleeding,or liver transplantation,when there is an obligate period of *** hallmark of IRI comprises mitochondrial dysfunction,which generates reactive oxygen species,and cell death through necrosis or ***(CsA),which is a well-known immunosuppressive agent that inhibits calcineurin,has the additional effect of inhibiting the mito-chondrial permeability transition pore(mPTP),thereby,preventing mitochondrial swelling and ***-811,which is the nonimmunosuppressive analog of CsA,has a similar effect on the *** this study,we tested the effect of both agents on mitigating warm hepatic IRI in a murine *** and methods:Before ischemic insult,the mice were administered with intraperitoneal normal saline(control);CsA at 2.5,10,or 25 mg/kg;or NIM-811 at 10 mg/***,the mice were subjected to partial warm hepatic ischemia by selective pedicle clamping for 60 min,followed by 6 h of recovery after *** alanine transaminase(ALT)was measured,and the liver tissue was examined histologically for the presence of apoptosis and the levels of inflammatory ***:Compared with the control mice,the mice treated with 10 and 25 mg/kg of CsA and NIM-811 had significantly lower ALT levels(P<0.001,0.007,and 0.031,respectively).Moreover,the liver tissue showed reduced histological injury scores after treatment with CsA at 2.5,10,and 25 mg/kg and NIM-811(P=0.041,<0.001,0.003,and 0.043,respectively)and significant decrease in apoptosis after treatment with CsA at all doses(P=0.012,0.007,and<0.001,respectively).Levels of the pro-inflammatory cyto-kines,particularly interleukin(IL)-1β,IL-2,IL-4,IL-10,and keratinocyte chemoattractant/human growth-regulated oncogene significantly decreased in the mice treated with the highest dose of CsA(25 mg/kg)than those in the control ***

关键词： Cyclosporine(CsA) Cyclosporine analogue Liver surgery Ischemia-reperfusion injury(IRI) Animal model NIM-811

Strategies on the application of stem cells based therapies for the treatment of optic neuropathies

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Neural Regeneration Research 2021年第6期16卷 1190-1191页

作者： sanaz Behtaj Maksym Rybachuk School of Engineering and Built Environment Griffith UniversityParklands DriveSouthportQueenslandAustralia Department of Cellular Biotechnology Cell Science Research CentreRoyan Institute for BiotechnologyIsfahanIran Queensland Micro-and Nanotechnology Centre Griffith UniversityNathanQueenslandAustralia School of Engineering and Built Environment Griffith UniversityNathanQueenslandAustralia

The retinal ganglion cells(RGCs)are not able to regenerate following optic nerve injury resulting in an irreversible vision loss in patients with optic neuropathies including *** findings in ocular regeneration have opened promising avenues to apply stem cell-based modalities to restore vision in progressive optic *** cellbased therapies can help to improve retinal regeneration by solving two major problems:(1)by preventing secondary degeneration of RGCs and preserving the remaining vision,and(2)by replacing degenerated RGCs and promoting RGC axon regeneration in the damaged area.

关键词： degeneration treatment irreversible

Nuclear atypia grading in breast cancer histopathological images based on CNN feature extraction and LSTM classification

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CAAI Transactions on Intelligence Technology 2021年第4期6卷 426-439页

作者： sanaz Karimi Jafarbigloo Habibollah Danyali Departtnent of Electrical and Electronics Engineering Shifa2 Univetsity of TechnologyShirazIran Department of Electrical Engtneering-Comtnunication System Shitaz Univetsity of TechnologyShirazIran

Early diagnosis of breast cancer,the most common disease among women around the world,increases the chance of treatment and is highly *** atypia grading in histopathological images plays an important role in the final diagnosis and grading ofbreast *** images by pathologists is a time consuming and subjective ***,the existence of a computer-aided system for nuclear atypia grading is very useful and necessary;In this stud%two automatic systems for grading nuclear atypia in breast cancer histopathological images based on deep learning methods are proposed.A patch-based approach is introduced due to the large size of the histopathological images and restriction of the training *** the proposed system I,the most important patches in the image are detected first and then a three-hidden-layer convolutional neural network(CNN)is designed and trained for feature extraction and to classify the patches *** proposed system II is based on a combination of the CNN for feature extraction and a two-layer Long short-term memoty(LSTM)network for *** LSTM network is utilised to consider all patches of an image simultaneously for image *** simulation results show the efficiency of the proposed systems for automatic nuclear atypia grading and outperform the current related studies in the literature.

关键词： breast cancer CNN histopathological image LSTM networks nuclear atypia

Review on application of nanoparticles for EOR purposes: A critical review of the opportunities and challenges

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Chinese Journal of Chemical Engineering 2019年第2期27卷 237-246页

作者： Yousef Kazemzadeh sanaz Shojaei Masoud Riazi Mohammad Sharifi Department of Petroleum Engineering Amirkabir University of Technology Tehran Polytechnic Enhanced Oil Recovery (EOR) Research Centre IOR/EOR Research Institute Shiraz University Petroleum Engineering Department School of Chemical and Petroleum Eng. Shiraz University

Nanoparticles have already gained attentions for their countless potential applications in enhanced oil ***-sized particles would help to recover trapped oil by several mechanisms including interfacial tension reduction, impulsive emulsion formation and wettability alteration of porous media. The presence of dispersed nanoparticles in injected fluids would enhance the recovery process through their movement towards oil–water interface. This would cause the interfacial tension to be reduced. In this research, the effects of different types of nanoparticles and different nanoparticle concentrations on EOR processes were investigated. Different flooding experiments were investigated to reveal enhancing oil recovery mechanisms. The results showed that nanoparticles have the ability to reduce the IFT as well as contact angle, making the solid surface to more water wet. As nanoparticle concentration increases more trapped oil was produced mainly due to wettability alteration to water wet and IFT reduction. However, pore blockage was also observed due to adsorption of nanoparticles, a phenomenon which caused the injection pressure to increase. Nonetheless, such higher injection pressure could displace some trapped oil in the small pore channels out of the model. The investigated results gave a clear indication that the EOR potential of nanoparticle fluid is significant.

关键词： Enhance oil recovery Nanofluid injection Nanoparticle Interfacial tension Wettability alteration Pore blockage

Highly stable Ga-doped ZnO/polystyrene nanocomposite film with narrow-band cyan emission

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Journal of Semiconductors 2022年第12期43卷 46-54页

作者： sanaz Alamdari Majid Jafar Tafreshi Morteza Sasani Ghamsari Department of Nanotechnology Faculty of New Sciences and TechnologiesSemnan UniversitySemnan35131-19111Iran Faculty of Physics Semnan University35195-363SemnanIran Photonics&Quantum Technologies Research School Nuclear Science and Technology Research Institute11155-3486TehranIran

In the present study,a simple method for the preparation of a luminescent flexible gallium doped zinc oxide(GZO)/polystyrene nanocomposite film was *** prepared GZO powder was characterized through different optical and structural *** XRD study revealed the existence of a wurtzite structure with no extra oxide ***-mapping,EDX,FTIR and XPS analyses were used to confirm the presence of elements and the several groups present in the *** excitations of UV,the prepared hybrid nanocomposite showed a strong cyan emission with narrow full width at half the maximum value(20 nm)that has not been reported before.X-ray and laser-induced luminescence results of the hy-brid film revealed novel blue-green emission at room *** prepared composite film showed a strong scintillation re-sponse to ionizing *** strong emissions,very weak deep-level emissions,and low FWHM of composite indicate the de-sirable optical properties with low-density structural defects in the GZO composite ***,the prepared hybrid film can be considered to be a suitable candidate for the fabrication of optoelectronic devices.

关键词： Ga doped ZnO cyan emission narrow-band emission nanocomposite

Local scour around submerged angled spur dikes under ice cover

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International Journal of Sediment Research 2023年第6期38卷 781-793页

作者： Guowei Li Jueyi Sui sanaz Sediqi Mauricio Dziedzic School of Engineering University of Northern British Columbia

Local scour is a phenomenon leading to the localized lowering of the channel bed due to the imbalance of sediment *** spur dikes protrude into the natural channels,local scour could be *** estimation of local scour around spur dikes is crucial for the effectiveness of erosion control and prevention and habitat enhancement *** the current study,the correlations between the maximum scour depth and the overtopping ratio,spur dike dimensions,ice cover roughness,and grain size of the bed material are *** both open channel and ice-covered flow conditions,a variety of experiments were done in a large-scale outdoor flume with different experimental *** results revealed that the scour depths around submerged spur dikes increased with increases in the densimetric Froude number and the decreases in the overtopping ratio and alignment *** maximum scour depth around a submerged angled vertical wall spur dike is significantly affected by the presence of an ice cover on the water surface,namely,the rougher the cover,the deeper the scour *** on data collected from the laboratory experiments,an existing maximum scour depth estimation equation has been modified to consider the influence of the cover condition and the submergence *** calculated results showed high accuracy in estimation of the measured data.

关键词： Ice cover Local scour Submerged spur dike Alignment angle

Comparison of therapeutic effects of topical azithromycin solution and systemic doxycycline on posterior blepharitis

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International Journal of Ophthalmology(English edition) 2016年第7期9卷 1016-1019页

作者： Mehdi Zandian Neda Rahimian sanaz Soheilifar Department of Ophthalmology Faculty of MedicineHamadan University of Medical sciences Endocrine Research Center Research Institute for Endocrine Sciences Shahid Beheshti University of Medical Sciences Department of Orthodontics Faculty of Dentistry Hamadan University of Medical Sciences

AIM：To compare the effect of azithromycin drop and doxycyciine capsule on treatment of posterior ***：Fifty patients（100 eyes） with moderate posterior blepharitis,randomly divided into two therapeutic groups;all the patients got warm eyelid compress and massage three times a day for *** addition the first group got azithromycin 1% drop,twice daily for 1wk and then one drop daily for *** second group got oral doxycyciine 100 mg daily for *** the end of the research,patients＇ signs and symptoms were compared ***,Chi-square and MannWhitney tests were used for statistical ***：Topical therapy with azithromycin and oral therapy with doxycyciine relieved signs and symptoms after *** were no significant differences between symptoms healing rate and foreign body sensation healing in these two groups（P〉0.05）.However,azithromycin drop was more effective in reduction of eye redness and doxycyciine was more effective in meibomian glands plugging healing and reducing the corneal ***：Topical azithromycin could have similar effects as oral doxycyciine on posterior blepharitis in improving subjective ***,doxycyciine can reduce objective signs such as ocular surface staining and meibomian gland plugging more than azithromycin.

关键词： blepharitis azithromycin doxycycline