Using directed mutagenesis and phage display on a soluble fragment of the human immunoglobulin superfamily receptor IlT2(synonyms:lIR1,MIR7,CD85j),we have selected a range of mutants with binding affinities enhanced b...
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Using directed mutagenesis and phage display on a soluble fragment of the human immunoglobulin superfamily receptor IlT2(synonyms:lIR1,MIR7,CD85j),we have selected a range of mutants with binding affinities enhanced by up to 168,000-fold towards the conserved region of major histocompatibility complex(MHC)class I *** in a dimeric form,either by chemical cross-linking with bivalent polyethylene glycol(PEG)derivatives or as a genetic fusion with human IgG Fc-fragment,the mutants exhibited a further increase in ligand-binding strength due to the avidity effect,with resident half-times(t1/2)on the surface of MHC I-positive cells of many *** novel compounds antagonized the interaction of CD8 co-receptor with MHC I in vitro without affecting the peptide-specific binding of T-cell receptors(TCRs).In both cytokine-release assays and cell-killing experiments the engineered receptors inhibited the activation of CD8^(+)cytotoxic T lymphocytes(CTls)in the presence of their target cells,with subnanomolar potency and in a dose-dependent *** a selective inhibitor of CD8^(+)CTl responses,the engineered high affinity IlT2 receptor presents a new tool for studying the activation mechanism of different subsets of CTls and could have potential for the development of novel autoimmunity therapies.
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