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2012年中国菌物学会学术年会

作者： M.J.Najafzadeh a.h.g gerrits van den ende V.A.Vicente g.S.de hoog CBS-KNAW Fungal Biodiversity Centre Utrechtthe Netherlands Department of Parasitology and Mycology Ghaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands Department of Basic Pathology Federal University of ParanaCuritibaPRBrazil peking University health Science Center Research Center for Medical Mycology Sun Yat-sen Memorial hospital Sun Yat-sen University

＜正＞Cyphellophora is a genus of black yeast-like fungi characterised by having simple phialides with multiseptate *** from SSU and LSU data,Cyphellophora was found to be located in a well-supported clade within the Chaetothyriales comprising a number of species occurring on human skin and nail. Cyphellophora is phylogenetically close to Phialophora europaea,*** and ***,though morphologically these species produce single-celled phialoconidia rather than multiseptate ones. Pseudomicrodochium suttonii and *** have dark colonies and phylogenetically fit in with Cyphellophora;the type species of Pseudomicrodochium,***,has similar,septate conidia but has a hyaline *** the present study,multilocus phylogenetic analyses were combined with morphology and *** of the internal transcribed spacer region,the DNA dependent RNA polymeraseⅡlargest subunit and the partial beta tubulin gene were analysed for a set of 30 *** novel species,Cyphellophora pauciseptata and Phialophora ambigua were *** eucalypti was reduced to synonymy of C. *** role of the studied fungi between colonization and infection of human skin was discussed. Putative virulence factors for these black yeast-like fungi were hypothesized to be the ability to assimilate monoaromatic hydrocarbons,to produce melanin pigments,and to tolerate the temperature of epidermal human *** identification and typing methods Amplified Fragment Length Polymorphism and Rolling Circle Amplification were used to diagnose Cyphellophora and *** vitro activities of eight antifungal drugs against 81 isolates of Cyphellophora and its relatives in Phialophora were *** black yeast-like organisms are susceptible in vitro to the newer azoles and to micafungin,but show resistance to amphotericin-B,fluconazole and terbinafin.

关键词： Cyphellophora Phialophora Taxonomy

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Diversity and delimitation of Rhizopus microsporus

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Fungal Diversity 2014年第1期64卷 145-163页

作者： Somayeh Dolatabadi grit Walther a.h.g.gerrits van den ende g.S.de hoog CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508 AD UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Institute of Microbiology Department of Microbiology and Molecular BiologyUniversity of JenaJenaGermany Leibniz-Institute for Natural Product Research and Infection Biology-hans-Knöll-Institute Jena Microbial Resource CollectionJenaGermany Research Center for Medical Mycology Peking University Health Science CenterBeijingChina Sun Yat-sen Memorial hospital Sun Yat-sen UniversityGuangzhouChina Shanghai Institute of Medical Mycology Changzheng HospitalSecond Military Medical UniversityShanghaiChina

Rhizopus microsporus has been used for centuries in the production of oriental fermented foods,but the species is also known as a toxin producer and from severe human *** study the diversity and species delimitation of Rhizopus microsporus,48 isolates from the reference collection of the CBS-KNAW Fungal Biodiversity Centre,comprising nine environmental,ten clinical,and 23 foodborne strains,in addition to six strains from unknown sources and representing all existing varieties of the species,were *** diversity was based on the internal transcribed spacer(ITS),and on a part of the actin(ACT)and translation elongation factor 1-α(TEF)*** in physiological properties were assessed including temperature *** morphology was studied,mating type tests were performed,and MALDI-ToF profiles were *** and food-associated strains as well as members of different varieties mated successfully and consequently they belong to a single biological *** differences did not match with any other parameter *** on these results the varieties of Rhizopus microsporus are reduced to synonyms.

关键词： Rhizopus microsporus Phylogeny Taxonomy Physiology MALDI-ToF Mating type Foodborne fungi Medical mycology Sporulation

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Multilocus analysis of the Exophiala jeanselmei clade containing black yeasts involved in opportunistic disease in humans

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Fungal Diversity 2014年第2期65卷 3-16页

作者： Jingsi Zeng Peiying Feng a.h.g.gerrits van den ende Liyan Xi M.J.harrak g.S.de hoog Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre P.O.Box 851673508 AD Utrechtthe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands Department of Dermatology and Venereology Union HospitalTongji Medical CollegeHuazhong Science and Technology UniversityWuhanChina Third Affiliated hospital Sun Yat-sen UniversityGuangzhouChina Sun Yat-sen Memorial hospital Sun Yat-sen UniversityGuangzhouChina Peking University health Science Center Research Center for Medical MycologyBeijingChina Shanghai Institute of Medical Mycology Changzheng HospitalSecond Military Medical UniversityShanghaiChina

To confirm species delimitations in the‘jeanselmeiclade’in the Chaetothyriales,four independent markers were analysed,and phylogenetic trees were reconstructed using different *** isolation within the complex and reproductive modes in the species involved were determined,in order to explore specific borderlines using genealogical Concordance Phylogenetic Species Recognition(gCPSR).Sequences of the Internal Transcribed Spacer(ITS),partial Translation Elongation Factor 1-α(TEF1),β-Tubulin(BT2),and Actin(ACT1)genes were analysed for a set of 81 *** reconstruction was performed using neighbour-joining(NJ),maximum parsimony(MP),maximum likelihood(ML)and Bayesian analysis(BA)to evaluate the concordance of topologies obtained under different optimization *** among lineages was detected by Partition homogeneity Test(PhT).Most Exophiala species analysed in this study seemed to be preponderantly clonal.A group of species around Exophiala spinifera showed a tendency toward human pa.h.genicity,whereas species around *** were often associated with environments rich in *** the tree topologies were not completely identical when different algorithms were used,the four gene lineages were nearly congruent and the trees of the combined multilocus data set did not provide more phylogenetic information than those of separate data *** data are sufficient for identification of species in the‘jeanselmei-clade’.

关键词： Black yeasts Clonality Exophiala ITS diagnostics Phylogeny

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Taxonomy of Ochroconis, genus including opportunistic pa.h.gens on humans and animals

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Fungal Diversity 2014年第2期65卷 89-126页

作者： K.Samerpitak E.van der Linde h.-J.Choi a.h.g.gerrits van den ende M.Machouart C.gueidan g.S.de hoog CBS-KNAW Fungal Biodiversity Centre UtrechtThe Netherlands Department of Microbiology Faculty of MedicineKhon Kaen UniversityKhon KaenThailand Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Mycology Unit Biosystematics ProgrammePlant Protection Research InstituteAgricultural Research CouncilPretoriaSouth Africa Evangelisches und Johanniter Klinikum Niederrhein DuisburgGermany Service de Parasitologie-Mycologie CHU de BraboisVandœuvre-lès-NancyFrance Department Life Sciences Division of Microbial&Genomic DiversityNatural History MuseumLondonUK Peking University health Science Center Research Center for Medical MycologyBeijingChina Sun-Yat-sen Memorial hospital Sun Yat-sen UniversityGuangzhouChina Second Medical Military University ShanghaiChina

The genus Ochroconis(Sympoventuriaceae,Venturiales)is revised and currently contains 13 species for which the phylogenetic position has been determined using multilocus *** older generic name Scolecobasidium is considered to be of doubtful identity because the type specimen is *** the Ochroconis lineage,phylogenetic distances of all markers analyzed are exceptionally large,both between and within species.A new genus Verruconis is proposed for the neurotropic opportunist Ochroconis *** accepted within the lineages are keyed out on the basis of phenotypic *** ecological traits within each species are *** species are thermophilic and one of them is an important agent of infection in the brain,while Ochroconis is mesophilic,several species causing infections in cold-blooded animals.

关键词： Taxonomy Ecology Thermophilic fungi human infection Avian pa.h.gen Fish pa.h.gen Neurotropism

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Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection

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Fungal Diversity 2014年第2期65卷 17-45页

作者： Peiying Feng Qiaoyun Lu M.J.Najafzadeh a.h.g.gerrits van den ende Jiufeng Sun Ruoyu Li Liyan Xi V.A.Vicente Wei Lai Chun Lu g.S.de hoog Third Affiliated hospital Sun Yat-sen UniversityGuangzhouChina Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity CentreP.O.Box 851673508 AD Utrechtthe Netherlands Xiangyang Central hospital XiangyangChina Peking University health Science Center Research Center for Medical MycologyBeijingChina Department of Parasitology and Mycology and Cancer Molecular Pathology Research CenterGhaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands Key Laboratory of Tropical Disease Control Department of ParasitologyZhongshan School of MedicineSun Yat-sen UniversityGuangzhouChina Sun Yat-sen Memorial hospital Sun Yat-sen UniversityGuangzhouChina Department of Basic Pathology Federal University of ParanáCuritibaPRBrazil

Cyphellophora is a genus of black yeast-like fungi characterised by having simple phialides with multiseptate,curved *** from SSU and LSU data,Cyphellophora was found to be located in a well-supported clade within the Chaetothyriales comprising a number of species occurring on human skin and *** is phylogenetically close to Phialophora europaea,*** and ***,though morphologically these species produce singlecelled phialoconidia rather than multiseptate *** suttonii and *** have dark colonies and phylogenetically fit in with Cyphellophora;the type species of Pseudomicrodochium,***,has similar,septate conidia but has a hyaline *** the present study,multilocus phylogenetic analyses were combined with morphology and *** of the internal transcribed spacer region,the DNA dependent RNA polymerase II largest subunit and the partial beta tubulin gene were analysed for a set of 30 *** novel species,Cyphellophora pauciseptata and Phialophora ambigua were *** eucalypti was reduced to synonymy of *** role of the studied fungi between colonization and infection of human skin was *** virulence factors for these black yeast-like fungi were hypothesized to be the ability to assimilate monoaromatic hydrocarbons,to produce melanin pigments,and to tolerate the temperature of epidermal human skin.

关键词： Black yeasts Chaetothyriales Cyphellophora Phialophora Superficial infection Taxonomy

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Phylogeny,ecology and taxonomy of systemic pa.h.gens and their relatives in Ajellomycetaceae(Onygenales):Blastomyces,Emergomyces,Emmonsia,Emmonsiellopsis

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Fungal Diversity 2018年第3期90卷 245-291页

作者： Yanping Jiang Karolina Dukik Jose F.Munoz Lynne Sigler Ilan S.Schwartz Nelesh P.govender Chris Kenyon Peiying Feng Bert gerrits van den ende J.Benjamin Stielow Alberto M.Stchigel hongguang Lu Sybren de hoog Department of Dermatology The Affiliated HospitalGuizhou Medical UniversityGuiyangChina Westerdijk Fungal Biodiversity Institute UtrechtThe Netherlands Center of Expertise in Mycology of RadboudUMC/Canisius Wilhelmina hospital NijmegenThe Netherlands Institute of Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Broad Institute of MIT and harvard CambridgeMAUSA and Biological Sciences EdmontonABCanada Division of Infectious Diseases Department of MedicineFaculty of Medicine and DentistryUniversity of AlbertaEdmontonABCanada global health Institute Faculty of Medicine and Health SciencesUniversity of AntwerpAntwerpBelgium A Division of the National Health Laboratory ServiceJohannesburgSouth Africa Sexually Transmitted Infection Unit Institute of Tropical MedicineAntwerpBelgium Third Affiliated hospital Sun Yat-Sen UniversityGuangzhouChina Thermo Fisher Scientific LandsmeerThe Netherlands Mycology Unit and IISPV University Rovira i VirgiliReusTarragonaSpain

The family Ajellomycetaceae(Onygenales)includes mammal-associated pa.h.gens within the genera Blastomyces,Emmonsia,histoplasma and Paracoccidioides,as well as the recently described genera,Emergomyces that causes disease in immunocompromised hosts,and Emmonsiellopsis,known only from *** further assess the phylogenetic relationships among and between members of these genera and several previously undescribed species,we sequenced and analyzed the DNA-directed RNA polymerase II(rPB2),translation elongation factor 3-a(TEF3),b-tubulin(TUB2),28S large subunit rDNA(LSU)and the internal transcribed spacer regions(ITS)in 68 strains,in addition to morphological and physiological *** better understand the thermal dimorphism among these fungi,the dynamic process of transformation from mycelial to yeast-like or adiaspore-like forms was also assessed over a range of temperatures(6–42C).Molecular data resolved the relationships and recognized five major well-supported lineages that correspond largely to the genus ***,typified by Emmonsia parva,is a synonym of Blastomyces that also accommodates Blastomyces helicus(formerly Emmonsia helica).Emmonsia crescens is phylogenetically distinct,and found closely related to a single strain from soil without known *** silverae,Emergomyces canadensis,Emergomyces europaeus and Emmonsia sola are newly *** all of the taxa are associated with human and animal *** crescens,*** and *** are prevalently associated with pulmonary disease in humans or *** helicus,***,Emergomyces africanus,***,***,*** and ***(formerly Emmonsia pasteuriana)are predominantly found in human hosts with immune disorders;no animal hosts are known for these species except ***.

关键词： Ajellomycetaceae Onygenales Phylogeny Ecology Thermal dimorphism Systemic infection Taxonomy

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两性霉素B联合5-FC对致原发性脑暗色丝孢霉病的暗色真菌体外药物相互作用研究

两性霉素B联合5-FC对致原发性脑暗色丝孢霉病的暗色真菌体外药物...

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中国菌物学会2015年学术年会

作者：邓淑文潘伟华廖万清 g.S.de hoog a.h.g.gerrits van den ende R.g.Vitale,h.Rafati A.h.van der Lee A.J.M.M.Rijs P.E.Verweij S.Seyedmousavi 上海市医学真菌分子生物学重点实验室荷兰CBS真菌多样性研究中心上海长征医院皮肤科

原发性脑暗色丝孢霉病是一个致命性真菌感染,主要有亲神经性的暗色真菌包括Cladophialophora bantiana,Rhinocladiella mackenziei和Exophiala dermatitidis感染所致.即使联合抗真菌药物治疗及手术去除病灶,该病死亡率依然高达50-70%.因... 详细信息

原发性脑暗色丝孢霉病是一个致命性真菌感染,主要有亲神经性的暗色真菌包括Cladophialophora bantiana,Rhinocladiella mackenziei和Exophiala dermatitidis感染所致.即使联合抗真菌药物治疗及手术去除病灶,该病死亡率依然高达50-70%.因此,寻找更有效的治疗方案迫在眉睫.我们体外研究发现,两性霉素B联合5-FC对致原发性脑暗色丝孢霉病的暗色真菌体外药物相互作用,所有测试菌株平均FIC指数范围0.25 to 0.38,表明具有协同作用。另外,采用Bliss独立分析,也观察到明显的协同作用,?E总数范围为90.2-698.61%。结果表明两性霉素B联合5-FC对原发性脑暗色丝孢霉病的暗色真菌具有协同作用,仍然需要动物实验和临床研究进一步观察证实,为临床治疗原发性脑暗色丝孢霉病提供有效的治疗方案。

关键词：暗色丝孢霉病两性霉素b 5-氟胞嘧啶

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肠套叠水压灌肠复位法的成功与症状持续时间的关系

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世界核心医学期刊文摘（儿科学分册） 2006年第A3期2卷 35-36页

作者： van den ende E.D. Allema J.h. hazebroek F.W.J. Breslau P.J. 王一飞 Department of Surgery Red Cross and Juliana Children' s Hospital Sportlaan 600 2566 MJ The Hague Netherlands

Background: It is widely believed that hydrostatic reduction of intussusception is less successful in children with prolonged symptoms prior to presentation. Aim: To prospectively evaluate success in relation to duration of symptoms. Methods: Prospective study in which children, regardless of symptom duration, underwent an attempt at hydrostatic reduction. Results: Of 113 children presenting with intussusception, 16 had peritonitis and required immediate laparotomy. A hydrostatic reduction was attempted in 97 and was successful in 77 (79% ). There were 26 successful reductions with symptoms hours (81% ), 30 with symptoms for 12-24 hours (81% ), and 21 with symptoms >24 hours (75% ). Conclusion: The success rate with hydrostatic reduction was not significantly influenced by symptom duration.

关键词：水压灌肠复位症状持续时间合并腹膜炎时间相关

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