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Controlling the Cassie-to-Wenzel Transition: an Easy Route towards the Realization of Tridimensional Arrays of Biological Objects

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Nano-Micro Letters 2014年第3期6卷 280-286页

作者： G.Ciasca M.Papi M.Chiarpotto A.De Ninno E.Giovine G.Campi a.gerardino M.De Spirito L.Businaro Istituto di Fisica Universit'a Cattolica S. Cuore L.go Francesco Vito 1 I-00168 Roma Italy Istituto di Fotonica e Nanotecnologie - CNR Via Cineto Romano 42 Roma 00156 Italy Institute of Crystallography – CNR Via Salaria Km 29 0016 Monterotondo Roma Italy

In this paper we provide evidence that the Cassie-to-Wenzel transition, despite its detrimental effects on the wetting properties of superhydrophobic surfaces, can be exploited as an effective micro-fabrication strategy to obtain highly ordered arrays of biological objects. To this purpose we fabricated a patterned surface wetted in the Cassie state, where we deposited a droplet containing genomic DNA. We observed that, when the droplet wets the surface in the Cassie state, an array of DNA filaments pinned on the top edges between pillars is formed. Conversely, when the Cassie-to-Wenzel transition occurs, DNA can be pinned at different height between pillars. These results open the way to the realization of tridimensional arrays of biological objects.

关键词： Superhydrophobic patterned surfaces Cassie-to-Wenzel transition DNA arrays

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Environmentally Safe Surfactants in Cosmetic/Detergent Formulations:Risk of Microbial Contamination and Possible Solutions

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China Detergent & Cosmetics 2017年第1期2卷 54-60页

作者： gerardino D'Errico Department of Chemical Sciences University of Naples "Federico Ⅱ"

Eco-and bio-compatible surfactants could potentially increase the risk of microbial contamination of the formulations in which they are included. In the present work, we characterize the microstructure of an hair conditioner formulation based on the bio-compatible cationic surfactant. Behenamidopropyldimethylamine(BAPDA) and evaluated its in vitro microbiological stability. The mixture presents an isotropic micellar structure, as revealed by Electron Paramagnetic Resonance(EPR) spectroscopy. The Challenge Test results show that the formulation is not sel-preserving, requiring the addition of selected preservatives, whose concentration has to be carefully optimized, to inhibit the growth of contaminating micro-organisms.

关键词： cosmetic formulation surfactant preservative Electron Paramagnetic Resonance Challenge Test

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Controlling DNA Bundle Size and Spatial Arrangement in Selfassembled Arrays on Superhydrophobic Surface

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Nano-Micro Letters 2015年第2期7卷 146-151页

作者： Gabriele Ciasca Massimiliano Papi Valentina Palmieri Michela Chiarpotto Simone Di Claudio Adele De Ninno Ennio Giovine Gaetano Campi Annamaria.gerardino Luca Businaro Marco De Spirito Istituto di Fisica Universitá Cattolica SC L.go Francesco Vito 100168 Rome Italy Istituto di Fotonica e Nanotecnologie-CNR Via Cineto Romano 42 00156 Rome Italy Institute of Crystallography-CNR Via Salaria Km 290016 Monterotondo Rome Italy

The use of superhydrophobic surfaces(SHSs) is now emerging as an attractive platform for the realization of one-dimensional(1D) nanostructures with potential applications in many nanotechnological and biotechnological *** this purpose, a strict control of the nanostructures size and their spatial arrangement is highly required. However, these parameters may be strongly dependent on the complex evaporation dynamics of the sessile droplet on the SHS. In this work, we investigated the effect of the evaporation dynamics on the size and the spatial arrangement of self-assembled 1D DNA bundles. Our results reveal that different arrangements and bundle size distributions may occur depending on droplet evaporation stage. These results contribute to elucidate the formation mechanism of 1D nanostructures on SHSs.

关键词： Superhydrophobicity DNA array Cassie state Wenzel state 1D nanostructures Self-assembly

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Ultra-subwavelength phase-sensitive Fano-imaging of localized photonic modes

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Light(Science & Applications) 2015年第1期4卷 223-230页

作者： Niccolo Caselli Francesca Intonti Federico La China Francesco Riboli Annamaria.gerardino Wei Bao Alexander Weber Bargioni Lianhe Li Edmund H Linfield Francesco Pagliano Andrea Fiore Massimo Gurioli European Laboratory for Nonlinear Spectroscopy via Nello Carrara 150019Sesto Fiorentino(FI)Italy Department of Physics University of Florencevia Sansone 150019 Sesto Fiorentino(FI)Italy Department of Physics University of Trentovia Sommarive 1438123Povo(TN)Italy Institute of Photonics and Nanotechnology CNR00156 RomaItaly Molecular Foundry Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA School of Electronic and Electrical Engineering University of LeedsLeeds LS29JTUK COBRA Research Institute Eindhoven University of Technology5600 MB EindhovenThe Netherlands

Photonic and plasmonic devices rely on nanoscale control of the local density of optical states(LDOS)in dielectric and metallic *** tremendous progress in designing and tailoring the electric LDOS of nano-resonators requires an investigation tool that is able to access the detailed features of the optical localized resonant modes with deep-subwavelength spatial *** scenario has motivated the development of different nanoscale imaging ***,we prove that a technique involving the combination of scanning near-field optical microscopy with resonant scattering spectroscopy enables imaging the electric LDOS in nano-resonators with outstanding spatial resolution(λ/19)by means of a pure optical method based on light *** this technique,we investigate the properties of photonic crystal nanocavities,demonstrating that the resonant modes appear as characteristic Fano line shapes,which arise from ***,by monitoring the spatial variation of the Fano line shape,we locally measure the phase modulation of the resonant modes without the need of external heterodyne *** novel,deep-subwavelength imaging method allows us to access both the intensity and the phase modulation of localized electric ***,this technique could be implemented on any type of platform,being particularly appealing for those based on non-optically active material,such as silicon,glass,polymers,or metals.

关键词： nanocavity nanophotonics near-field phase retrieval resonant-scattering

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Helicobacter pylori infection in bleeding peptic ulcer patients after non-steroidal antiinflammatory drug consumption

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World Journal of Gastroenterology 2011年第40期17卷 4509-4516页

作者： Francesco Manguso Elisabetta Riccio Germana de Nucci Maria Luisa Aiezza.gerardino Amato Linda Degl'Innocenti Maria Maddalena Piccirillo Gianfranco De Dominicis Tara Santoro Elena Trimarco Antonio Balzano Complex Operating Unit of Gastroenterology AORN A Cardarelli 80131 Napoli Italy Complex Operating Unit of Pharmacy AORN A Cardarelli 80131 Napoli Italy Complex Operating Unit of Microbiology AORN A Cardarelli 80131 Napoli Italy Complex Operating Unit of Pathology AORN A Cardarelli 80131 Napoli Italy

AIM： TO establish the prevalence of He/icobacterpy/on （H. pylori） infection in patients with a bleeding peptic ulcer after consumption of non-steroidal antiinflammatory drugs （NSAIDs）.METHODS： A very early upper endoscopy was performed to find the source of upper gastrointestinal bleeding and to take biopsy specimens for analysis of H. pylori infection by the rapid urease （CLO） test, his- tological examination, and bacterial culture. TgG anti- CagA were also sought. The gold standard for identifying H. pylori infection was positive culture of biopsy specimens or contemporary positivity of the CLO test and the presence of H. pylori on tissue ***： Eighty patients, 61 males （76.3%）, mean age 61.2 ~ 15.9 years, were consecutively enrolled. Forty-seven （58.8%） patients occasionally consumed NSAIDs, while 33 （41.3%） were on chronic treatment with low-dose aspirin （LD ASA）. Forty-four （55.0%） patients were considered infected by H. pylori. The infection rate was not different between patients who occasionally or chronically consumed NSAIDs. The culture of biopsy specimens had a sensitivity of 86.4% and a specificity of 100%; corresponding figures for histological analysis were 65.9% and 77.8%, for the CLO test were 68.2% and 75%, for the combined use of histology and the CLO test were 56.8% and 100%, and for IgG anti-CagA were 90% and 98%. The high- est accuracy （92.5%） was obtained with the culture of biopsy ***： Patients with a bleeding peptic ulcer after NSAID/LD ASA consumption frequently have H. pylori infection. Biopsy specimen culture after an early upper gastrointestinal tract endoscopy seems the most efficient test to detect this infection.

关键词： Helicobacter pylori Helicobacter pylori in-fection Low-dose aspirin Non-steroidal antiinflamma-tory drugs Peptic ulcer hemorrhage Endoscopy

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