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Simultaneous Determination of Atorvastatin and Glimepiride by LC-MS/MS in Human Plasma and Its Application to a Pharmacokinetic Study

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American Journal of Analytical Chemistry 2012年第8期3卷 559-569页

作者： Kishore Kumar Hotha Narasimha Reddy Yarramu Thriveni Kandibedala Vijaya.bharathi Dasari Venkateswarlu Vobalaboina Bioanalytical Department Integrated Product Development Dr. Reddy’s Laboratories Ltd Bachupalli Hyderabad India

The aim of the proposed research work was to develop and validate a simple, selective high sensitive and high-throughput assay for the simultaneous estimation of Atorvastatin and Glimepiride in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Atorvastatin–Glimepiride combines a competitive inhibitor of HMG-CoA reductase and a sulfonylurea anti-diabetic drug. The purpose of this study was to develop single method for Atorvastatin and Glimepiride in plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) that would result into a simultaneous estimation of Atorvastatin and Glimepiride avoiding acid –lactone inter conversions right from sample collections to analysis on the LC-MS/MS. Sample collection procedure optimized for Atorvastatin holds good for Glimepiride, hence resulting into a simultaneous estimation of Atorvastatin and Glimepiride. Liquid-liquid extraction and liquid chromatography coupled to positive ion mode tandem mass spectrometry was used to develop the method and was validated according to US FDA guidelines. The calibration curves for two analytes were linear (R2 ≥ 0.9950, n = 4) over the concentration range of 0.2 - 30 ng/mL for Atorvastatin and 1 - 250 ng/mL for Glimepiride. Mean extraction recoveries 80.34 ± 9.43 for Atorvastatin and 88.19 ± 7.13 for Glimepiride. Intra- and inter-run mean percent accuracy was between 85% - 115% and percent imprecision was ≤15%. Stability studies revealed that Atorvastatin and Glimepiride were stable in plasma during bench top (10.5 h at room temperature), in Injector (47.5 h), at the end of three successive freeze and thaw cycles and long term at -65℃ ± 15℃ for 114 days. The method was successfully applied to the study of pharmacokinetics of Atorvastatin and Glimepiride in healthy volunteers. Simultaneous estimation of Atorvastatin and Glimepiride is cost effective, reduces analysis cycle time, enables effective utilization of resources and reduces bleeding burden on human vo

关键词： Atorvastatin Glimepiride LC-MS/MS Method Validation Human Plasma and Pharmacokinetic Studies

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Molecular basis of the anti-inflammatory potential of a diarylheptanoid in murine macrophage RAW 264.7 cells

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Advances in Biological Chemistry 2013年第6期3卷 541-548页

作者： bharathi Raja Rajaganapathy Karthikeyan Thirugnanam Muthusamy Velusamy Shanmuganathan Anand Singaravelu Lakshmi Baddireddi Subadhra Tissue Culture and Drug Discovery Lab Centre for Biotechnology Anna University Chennai India Tissue Culture and Drug Discovery Lab Centre for Biotechnology Anna University Chennai India 2National Institute of Nutrition (Indian Council of Medical Research) Hyderabad India National Institute of Nutrition (Indian Council of Medical Research) Hyderabad India

Natural products play a significant role in human health in relation to the prevention and treatment of inflammatory disorders. In this study, we examined the molecular basis of the anti-inflammatory potential of a diarylheptonoid (DAH) isolated from Alpinia officinarum hexane extract (AOHE) with special emphasis on their ability to modulate the nuclear factor-κB (NF-кB) signaling involved in the inflammatory response. Measurement of Nitrite by Griess reaction which revealed the effect of DAH in RAW 264.7 macrophages showed an inhibition in the nitric oxide production through the suppression of inducible nitric oxide synthase (iNOS) gene level expression. NF-кB reporter gene assay suggests inhibition of NF-кB transcriptional activity, thus inhibiting LPS-induced phosphorylation and degradation of IкBα and a downregulation of NF-кB protein expression confirms the immunomodulatory effect of DAH. Furthermore, downregulation in the gene level expression of NF-кB signaling markers such as IL-1β, TNF-α and COX-2 suggests the anti-inflammatory potential of DAH via inhibition of NF-кB activation.

关键词： Diarylheptanoid NF-кB Lipopolysaccharide TNF-α COX-2 iNOS Cytokines

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美国生活用纸市场逐渐恢复并趋于平稳

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生活用纸 2023年第11期23卷 27-30页

作者： Sanna Sosa Sivashankari bharathi AFRY咨询公司

自2020年初新冠疫情暴发以来,北美生活用纸需求一直处于过山车般的起伏状态,市场需求出现了两位数的上下波动。2022年底的市场数据显示,消费者购买力受到压力使居家用(AH)生活用纸需求在下半年再次下滑。由于流动性增加和服务业客流量... 详细信息

自2020年初新冠疫情暴发以来,北美生活用纸需求一直处于过山车般的起伏状态,市场需求出现了两位数的上下波动。2022年底的市场数据显示,消费者购买力受到压力使居家用(AH)生活用纸需求在下半年再次下滑。由于流动性增加和服务业客流量的回归,居家外用(AfH)生活用纸市场在2022年中呈现复苏景象。目前,美国居家外用生活用纸市场已基本恢复到疫情之前的水平。居家用生活用纸市场正面临购买习惯的结构性变化、购买能力受到挑战、零售商品牌销售量增加以及节俭消费者的回归。为了了解2023年的美国生活用纸新动态,AFRY咨询公司对300多名美国消费者进行了调查,以了解他们的消费迁移性和消费习惯。生活用纸市场的波动会继续吗?美国消费者购买能力受到挑战会对生活用纸消费造成什么影响?美国是否回到了疫情前的服务业消费水平?是否存在长期消费迁移影响导致居家外用生活用纸需求的结构性变化?

关键词：购买习惯消费者购买力消费水平消费习惯生活用纸结构性变化服务业数据显示

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Matrix Representation of Bipartite Steinhaus graph on DNA Splicing System

Matrix Representation of Bipartite Steinhaus graph on DNA Sp...

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2012 Third International Conference on Theoretical and Mathematical Foundations of Computer Science(ICTMF 2012)

作者： S.Jeya.bharathi R.Anusha J.Suganya Devi Department of Mathematics Thiagarajar College of EngineeringMaduraiIndia

The notion of splicing system to model the recombinant behaviour of DNA molecules is introduced by Tom Head [7] in *** found that the bipartite steinhaus graphs are suitable model for DNA splicing *** this paper,we applied the concepts of bipartite Steinhaus graphs for DNA *** row generators have been introduced and its characterizations were presented using matrix.

关键词： Bipartite Steinhaus graph DNA splicing

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Malignant middle-cerebral artery territory infarction in tuberculous vasculitis

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Neuroimmunology and Neuroinflammation 2014年第1期1卷 95-97页

作者： Salvadeeswaran Meenakshi-Sundaram Sundararajan Srinivasan Somalinga Nagendran Karthik Suresh Pandi Alagappan Periyakaruppan bharathi Sundar Department of Neurosciences Apollo Speciality HospitalsMadurai 625020Tamil NaduIndia Department of Radiology Apollo Speciality HospitalsMadurai 625020Tamil NaduIndia

Intracranial large vessel involvement is an unusual complication of tuberculous *** authors report a 39-year-old female presenting with an episode of seizure,followed by rapid decline in sensorium without prominent systemic *** initial cranial magnetic resonance imaging revealed tuberculomata and patchy *** antituberculous therapy,she progressively worsened.A cranial computed tomography scan done following the worsening revealed a massive middle-cerebral artery(MCA)***,the patient died in spite of decompressive *** MCA territory infarct is a rare and potentially fatal complication of tuberculous meningitis.

关键词： Arteritis malignant middle-cerebral artery territory infarct tuberculous meningitis

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Determining the Minimum Inhibitory Concentration of Bacteriophages: Potential Advantages

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Advances in Microbiology 2013年第2期3卷 181-190页

作者： Aradhana Vipra Srividya Narayanamurthy Desai Raghu Patil Junjappa Panchali Roy Nethravathi Poonacha Pallavi Ravinder bharathi Sriram Sriram Padmanabhan Microbiology Team Gangagen Biotechnologies Pvt. Ltd. Raghavendra layout Opp. MEI Ltd. Bangalore India

The minimum inhibitory concentration (MIC) is the concentration at which an antibacterial agent experiences the complete inhibition of organism growth. Bacteriophages represent a rich and unique resource of anti-infectives to counter the growing world-wide problem of antibiotic resistance. In this study, we compared the host range of lytic bacteriophages and temperate phagesbelonging to various genera, namely Staphylococcus, E. coli and Salmonella, with a range of clinical isolates using two methods: the classical agar overlay method and a newly developed MIC method. MIC was only observed with isolates that were susceptible to phage infection, which correlated with the agar overlay assay, whereas no MIC was detected with isolates that were resistant to phage infection. The simple MIC method was useful in determining phage adsorption and host range, and detecting possible prophage contamination in phage preparations. Interestingly, this method was also applicable to strain differentiation through phage susceptibility testing using a 96-well, high throughput format that proved to be easy, cost-effective, fast and reliable.

关键词： Bacteriophage K MIC Phage 44AHJD Phage P954 Staphylococcus aureus Temperate Phage

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Efficacy of Isovolumic Acceleration in Assessing Right Ventricular Function in Pulmonary Hypertension

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International Journal of Clinical Medicine 2014年第12期5卷 674-680页

作者： V. S. bharathi Adikesava Naidu Otikunta Y. V. Subbareddy K. Laxman Rao Syed Imamuddin Ashok Thakkar Department of Cardiology Osmania General Hospital Hyderabad India Department of Clinical Trials Sahajanand Medical Technologies Pvt. Ltd. Surat India

Objective: The aim of the study was to determine usefulness of isovolumic acceleration to assess right ventricular function in patients with pulmonary hypertension. Background: Right ventricular dysfunction is a powerful predictor of adverse outcomes in patients with pulmonary hypertension. Although isovolumic acceleration is a sensitive and relatively load-independent parameter for ventricular contractile function, it has not been widely used. So, the aim of the study was to determine the usefulness of isovolumic acceleration in assessing right ventricular function in patients with pulmonary hypertension. Methods: A single center, prospective observational case-control study has been carried out for the period of one year at Osmania general hospital (Hyderabad). A total of 32 consecutive patients with pulmonary hypertension and 32 age matched healthy controls were recruited in the study as per the inclusion and exclusion criteria of the study. Receiver operator characteristic curves were used to study sensitivity, specificity, positive predictive value, negative predictive values as well as pearson’s correlation coefficients. Results: The results of the study showed that isovolumic acceleration has good significant correlation with right ventricular fractional area change (r = 0.554, p = 0.001) and tissue Doppler myocardial performance index (r = -0.599, p = 0.001) whereas a lesser but significant correlation was observed with tricuspid annular plane systolic excursion (r = 0.327, p = 0.034). Isovolumic acceleration showed higher specificity (81%) and positive predictive value (88%) in diagnosing right ventricular dysfunction in pulmonary hypertension compared to tricuspid annular systolic excursion velocity and tissue Doppler myocardial performance index parameters. Therefore isovolumic acceleration may be a better variable for diagnosing right ventricular dysfunction. Conclusions: Isovolumic acceleration is a sensitive and specific marker of right ventricular

关键词： Pulmonary Hypertension Isovolumic Acceleration Right Ventricular Dysfunction

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Safety and Efficacy of Ultra-Thin, Biodegradable Polymer Coated Sirolimus-Eluting Supralimus-Core Stents in Real-World Patients: Outcomes at 24-Month Follow-Up

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World Journal of Cardiovascular Diseases 2018年第11期8卷 523-532页

作者： Kala Jeethender Kumar Jain Subash Dasarapu Sai Satish Oruganti Jyotsna Maddury Srinivas Bhyravavajhala Rama Kumari Nuthalapati Vanaparthi Satya.bharathi Lakshmi Rajender Betham Anil Kumar Enikapalli Nizam’s Institute of Medical Sciences Hyderabad Telangana India

Aim: The purpose of this registry was to establish long-term safety and efficacy through implantation of Supralimus-Core sirolimus-eluting stents (SES) in real-world patients with coronary artery disease (CAD). Methods:The present registry was a retrospective, singe-arm, single-centre, investigator-initiated registry. A total of 372 consecutive patients were implanted with Supralimus-Core SES between January 2015 and November 2016. The primary endpoints were major adverse cardiac events (MACE), a composite of cardiac death, myocardial infarction (MI), target lesion revascularization (TLR) and target vessel revascularization (TVR) at 24 months. The secondary endpoints were all-cause death and all separate components of the primary endpoint. Additional endpoints included events of stent thrombosis classified as definite, probable, and possible stent thrombosis. Follow-ups were conducted at 30-days, 6-months, 12-months and 24-months after the index procedure. Results: The mean age of the registry population was 56.3 ± 11.1 years. Males constituted 276 (74.2%) patients. Hypertensives, diabetics, alcoholics, tobacco chewers and smokers comprised 198 (53.2%), 160 (43.0%), 93 (25.0%), 91 (24.5%) and 88 (23.7%) of the registry population, respectively. The mean length and diameter of stents implanted was 19.3 ± 8.8 mm and 2.9 ± 0.3 mm, respectively. At the 24-month follow-up, MACE was reported in 14 (3.8%) patients. Cardiac death, MI, TLR and TVR was reported in 7 (1.9%), 4 (1.1%), 3 (0.8%) and 4 (1.1%) patients, respectively. Overall stent thrombosis occurred in 4 (1.1%) patients. Conclusions: The low MACE rate of 3.8% at the 24-month follow-up indicates favorable long-term results after implantation of the ultra-thin strut Supralimus-Core SES in all-comer, real-world patients.

关键词： Biodegradable Polymer Cobalt-Chromium Coronary Artery Disease Drug Eluting Stents Percutaneous Coronary Intervention Sirolimus-Eluting Stent

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精神分裂症患者的心理社会干预(英文)

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上海精神医学 2011年第6期23卷 368-375页

作者：白哈拉桑黄楠陆峥上海同济大学附属同济医院心身科 200065 上海交通大学医学院附属精神卫生中心 200030

精神分裂症的治疗不仅包括药物治疗,还包括为患者提供支持、有效的信息、心理社会干预以及康复治疗。中国的研究者在精神分裂症患者非药物治疗领域进行着各项研究,而国外研究数据一致显示心理社会干预可以降低复发率和再住院率。尽管缓... 详细信息

精神分裂症的治疗不仅包括药物治疗,还包括为患者提供支持、有效的信息、心理社会干预以及康复治疗。中国的研究者在精神分裂症患者非药物治疗领域进行着各项研究,而国外研究数据一致显示心理社会干预可以降低复发率和再住院率。尽管缓解症状仍然是精神分裂症治疗的一个重要目标,但工作、学习、独立生活及社会化方面的功能损伤往往也是患者及其家属关注的重点。因此,精神分裂症患者更有可能从着重于减轻残疾的干预中获益。本文对中外有效应用于精神分裂症患者的主要心理社会干预进行综述,包括认知行为治疗、社会技能训练、家庭干预、认知纠正、心理教育、职业训练、危机干预及综合心理治疗;同时就中外心理社会干预的差异进行讨论。

关键词：精神分裂症非药物治疗心理社会干预

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系统评价与Meta分析:高氟牙膏与标准含氟牙膏的防龋效果比较

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中国口腔医学继续教育杂志 2018年第5期21卷 286-293页

作者： Abhinav Singh bharathi M.Purohit 辛月娇(译) 张珊珊(审) 郑树国(审) 不详北京大学口腔医院预防科 100081

目的:对比高氟牙膏(≥2500ppm)与标准含氟牙膏(≤1500ppm)的龋齿预防功效。材料与方法:采用随机对照试验(RCTs)和聚类随机试验比较高氟牙膏(≥2500ppm)和低浓度含氟牙膏(≤1500ppm)的防龋效果,随访时间至少6个月。随机效应模型用于评估... 详细信息

目的:对比高氟牙膏(≥2500ppm)与标准含氟牙膏(≤1500ppm)的龋齿预防功效。材料与方法:采用随机对照试验(RCTs)和聚类随机试验比较高氟牙膏(≥2500ppm)和低浓度含氟牙膏(≤1500ppm)的防龋效果,随访时间至少6个月。随机效应模型用于评估所使用的两种牙膏在龋齿增加方面的平均差异。固定效应模型用于评定高浓度含氟牙膏相较于低氟浓度牙膏的防龋效果。当结果存在异质性时,进行亚组分组和敏感性分析。设定p

关键词： Meta分析含氟牙膏防龋效果纳入标准高氟龋齿预防系统随机对照试验

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