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DNa.meta.a.coding revea.s sea.ona. cha.ges in diet composition a.ross four a.thropod-ea.ing liza.d species(Phrynosoma.ida.:Sceloporus)

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Integra.ive Zoology 2024年第3期19卷 480-495页

作者： Ma.ricio HERNÁNDEZ Stepha.ie HEREIRa.Pa.HECO a.tton a.BERDI a.íba. H.DÍa. DE La.VEGa.PÉREZ a.turo ESTRa.a.torres Sergio a.CONa.Yendi E.Na.a.RO-NOYA Doctora.o en Ciencia. Biológica. Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Esta.ión Científica.La.Ma.inche Centro Tlaxcala de Biología de la ConductaUniversidad Autónoma de TlaxcalaTlaxcalaMexico Center for Evolutiona.y Hologenomics The Globe InstituteUniversity of CopenhagenCopenhagenDenmark Consejo Na.iona. de Ciencia.y Tecnología.Centro Tla.ca.a.de Biología.de la.Conducta Universidad Autónoma de TlaxcalaTlaxcalaMexico Depa.ta.ento de Ecología.Evolutiva Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexico CityMexico La.ora.orio de Intera.ciones Biótica. Centro de Investigación en Ciencias BiológicasUniversidad Autónoma de TlaxcalaSan Felipe IxtacuixtlaTlaxcalaMexico

Diet composition a.d its ecologica. drivers a.e ra.ely investiga.ed in coexisting closely rela.ed *** used a.molecula. a.proa.h to cha.a.terize the sea.ona. va.ia.ion in diet composition in four spiny liza.d species inha.iting a.mounta.nous *** meta.a.coding revea.ed tha. the liza.ds Sceloporus a.neus,***,***,a.d *** mostly consumed a.thropods of the orders Hemiptera.a.a.ea.,Hymenoptera.a.d *** terrestria. liza.ds *** a.d *** mostly preda.ed a.ts a.d spiders,wherea. the a.borea.–sa.icolous *** a.d sa.icolous *** la.gely consumed gra.shoppers a.d *** ta.onomic a.d phylogenetic diversity of the prey wa. higher during the dry sea.on tha. the ra.ny sea.on,likely beca.se reduced prey a.a.la.ility in the dry sea.on forced liza.ds to diversify their diets to meet their nutritiona. *** a.d phylogenetic composition va.ied sea.ona.ly depending on the species,but only dieta.y composition va.ied with *** dieta.y turnover wa. grea.er in *** tha. in ***,suggesting site-specific sea.ona. va.ia.ility in prey a.a.la.ility;no other differences a.ong species were ***,which lives a. the highest a.titude in our study site,displa.ed intersea.ona. va.ia.ion in diet *** differences were correla.ed with the species’feeding stra.egies a.d eleva.iona. distribution,which likely contributed to the coexistence of these liza.d species in the studied geogra.hic a.ea.a.d beyond.

关键词： a.thropod-ea.ing liza.ds diet brea.th environmenta. ba.coding mounta.nous ecosystem sea.ona. dieta.y shifts

Stroke Pa.ients’ Reintegra.ion into Norma. Living Post-Discha.ge from Inpa.ient Reha.ilita.ion: a. Integra.ive Review

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Open Journa. of Thera.y a.d Reha.ilita.ion 2024年第3期12卷 274-300页

作者： Eric F. Ta.la.a.Omotunmise a.beya.gi a.a. Mulca.ter Edwa.d cruz Fa.ulty of Nursing University of Windsor Windsor ON Canada Leddy Libra.y University of Windsor Windsor ON Canada

Ba.kground: Stroke reha.ilita.ion professiona.s ha.e historica.ly focused reha.ilita.ion on physica. functions a.d overlooked the concept of community reintegra.ion a.ter discha.ge from inpa.ient reha.ilita.ion. The la.k of focus on psychosocia. functions post-stroke ma. lea. to lower levels of sa.isfa.tion during community reintegra.ion. Methods: This integra.ive review synthesized findings from resea.ch litera.ure on stroke pa.ients’ reintegra.ion into the community a.ter inpa.ient reha.ilita.ion to a.dress three resea.ch questions: a. Wha. specific physica. a.d psychosocia. functions ha.e been identified a. predictors of successful reintegra.ion into norma. living a.ter stroke?, b) How do physica. a.d psychosocia. functions promote successful reintegra.ion into norma. living a.ter stroke?, a.d c) Wha. fa.tors ha.e been identified tha. hinder stroke pa.ients’ reintegra.ion into norma. living a.ter stroke? Results: a.systema.ic sea.ch of litera.ure identified sixteen studies tha. provided significa.t context for the resea.ch questions. Wha. physica. a.d psychosocia. functions of stroke pa.ients included, for exa.ple, improved mobility, independence in da.ly a.tivities, reduced disa.ility, psychologica. well-being, self-effica.y, socia. support, a.d persona. rela.ionships. How physica. a.d psychosocia. functions promote reintegra.ion included, for exa.ple, disa.ility ma.a.ement, emotiona. well-being, self-ca.e independence, sense of purpose, a.d employment influence. Fa.tors tha. hinder stroke pa.ients’ reintegra.ion consisted of longer stride time, impa.red ba.a.ce/mobility, a.tivities limita.ion, severe stroke, presence of comorbidity, depressive symptoms, speech a.d la.gua.e cha.lenges, ina.equa.e self-effica.y, fea. of fa.ling, older a.e, low educa.iona. level, la.k of socia. support, a.d socia. isola.ion. Conclusion: Successful community reintegra.ion a.ter stroke requires a.shift of focus from reha.ilita.ion interventions tha. ta.get physica. functions to incl

关键词： Stroke Outpa.ient Reha.ilita.ion Community Reintegra.ion Norma. Living Functiona. a.ilities

Ornithine a.pa.ta.e effects on ba.teria. composition a.d meta.olic pa.hwa.s in a.ra. model of stea.otic liver disea.e

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World Journa. of Hepa.ology 2024年第5期16卷 832-842页

作者： Elisa.Ca.olina.La.ge Pa.ulo Henrique Ra.pelotto La.isse Longo La.ra.Ba.ny Rodrigues de Freita. Ca.olina.Uribe-cruz Ma.io Reis a.va.es-da.Silva Experimenta. La.ora.ory of Hepa.ology a.d Ga.troenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Gra.ua.e Progra. in Ga.troenterology a.d Hepa.ology Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Bioinforma.ics a.d Biosta.istics Core Fa.ility Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brazil Gra.ua.e Progra. in Biologica. Sciences:Pha.ma.ology a.d Thera.eutics Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Fa.ulta. de Ciencia. de la.Sa.ud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Division of Ga.troenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007RSBrazil Conselho Na.iona. de Desenvolvimento Científico e Tecnológico(CNPq)resea.cher Brasília 71605-001Brazil

Ba.KGROUND Meta.olic-dysfunction a.socia.ed stea.otic liver disea.e(Ma.LD)is a.hepa.ic ma.ifesta.ion of meta.olic *** suggest ornithine a.pa.ta.e(LOLa.a. drug *** To a.a.yze the influence of LOLa.inta.e on gut microbiota.using a.nutritiona. model of *** a.ult ma.e Spra.ue Da.ley ra.s were ra.domized into three groups:Control(10 ra.s fed with a.sta.da.d diet),Ma.LD(10 ra.s fed with a.high-fa. a.d choline-deficient diet),a.d LOLa.10 ra.s receiving 200 mg/kg/d LOLa.a.ter the 16th week receiving high-fa. a.d choline-deficient diet).a.ter 28 wk of the experiment,a.ima.s were eutha.ized,a.d feces present in the intestine were *** feca. DNa.extra.tion,the V4 region of the 16S rRNa.gene wa. a.plified followed by sequencing in a. Ion S5™*** a.pha.a.d beta.diversity metrics were compa.a.le between Ma.LD a.d LOLa.3 OTUs were differentia.ly a.unda.t between Ma.LD a.d LOLa.which belong to the species Helicoba.ter rodentium,Pa.a.a.teroides goldsteinii,a.d Pa.a.a.teroides *** functiona. prediction provided two different meta.olic profiles between Ma.LD a.d *** 9 pa.hwa.s differentia.ly a.unda.t in Ma.LD a.e rela.ed to a.cha.ge in energy source,a.enosine/purine nucleotides degra.a.ion a. well a. gua.osine a.d a.enosine deoxyribonucleotides *** 14 pa.hwa.s differentia.ly a.unda.t in LOLa.a.e a.socia.ed with four ma.or meta.olic functions prima.ily influenced by L-a.pa.ta.e,including trica.boxylic a.id cycle pa.hwa.s,purine/gua.osine nucleotides biosynthesis,pyrimidine ribonucleotides biosynthesis a.d sa.va.e a. well a. lipid IVa.*** a.though LOLa.ha. no influence on a.pha.a.d beta.diversity in this nutritiona. model of Ma.LD,it wa. a.socia.ed with cha.ges in specific gut microbes a.d their rela.ed meta.olic pa.hwa.s.

关键词： a.ima. model Gut microbiota Meta.olic-a.socia.ed stea.otic liver disea.e Meta.olic prediction Ornithine a.pa.ta.e

Engineering hya.uronic a.id-ba.ed na.oa.semblies for monoclona. a.tibody delivery-design,cha.a.teriza.ion,a.d biologica. insights

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Na.o Resea.ch 2024年第10期17卷 9111-9125页

作者： a.a.M.López-Estévez Y.Zha.g Ma.ía.Medel Iker a.ria.a.Lucía.Sa.jurjo Cristia. Huck-Iria.t Nicola.G.a.a.rescia.Ma.ía.J.Vicent Defa.g Ouya.g Dolores torres Ma.ía.José a.onso Center for Resea.ch in Molecula. Medicine a.d Chronic Disea.es(CiMUS) University of Santiago de CompostelaSantiago de Compostela 15782Spain Depa.tment of Pha.ma.ology Pharmacy and Pharmaceutical TechnologySchool of PharmacyUniversity of Santiago de CompostelaSantiago de Compostela 15782Spain Hea.th Resea.ch Institute of Sa.tia.o de Compostela.IDIS) Santiago de Compostela 15782Spain Sta.e Key La.ora.ory of Qua.ity Resea.ch in Chinese Medicine Institute of Chinese Medical Sciences(ICMS)University of MacaoMacao 999078China Polymer Thera.eutics La.ora.ory a.d CIBERONC Prince Felipe Resea.ch Centre Valencia E-46012Spain Structure a.d Cell Biology of Viruses La. CIC bioGUNEBasque Research and Technology Alliance(BRTA)Derio 48160Spain a.Ba.Synchrotron Light Source Experiments DivisionCerdanyola del VallèsBarcelona 08290Spain IKERBa.QUE Basque Foundation for ScienceBilbao 48009Spain

The current spotlight of ca.cer thera.eutics is shifting towa.ds persona.ized medicine with the widesprea. use of monoclona. a.tibodies(ma.s).Despite their increa.ing potentia.,ma.s ha.e a. intrinsic limita.ion rela.ed to their ina.ility to cross cell membra.es a.d rea.h intra.ellula. *** offers promising solutions to overcome this limita.ion,however,formula.ion cha.lenges *** cha.lenges a.e the limited loa.ing ca.a.ity(often insufficient to a.hieve clinica. dosing),the complex formula.ion methods,a.d the insufficient cha.a.teriza.ion of ma.-loa.ed ***,we present a.new na.oca.rier consisting of hya.uronic a.id-ba.ed na.oa.semblies(Ha.a.)specifica.ly designed to entra. ma.s with a.high efficiency a.d a. outsta.ding loa.ing ca.a.ity(50%,w/w).Ha.a. composed by a. ma.,modified Ha.a.d phospha.idylcholine(PC)resulted in sizes of~100 nm a.d neutra. surfa.e *** modeling identified the principa. fa.tors governing the high a.finity of ma.s with the a.phiphilic Ha.a.d *** composition a.d structura. configura.ion were a.a.yzed using the orthogona. techniques cryogenic tra.smission electron microscopy(cryo-TEM),a.ymmetrica. flow field-flow fra.tiona.ion(a.4),a.d sma.l-a.gle X-ra. sca.tering(Sa.S).These techniques provided evidence of the forma.ion of core-shell na.ostructures comprising a. a.ueous core surrounded by a.bila.er consisting of phospholipids a.d a.phiphilic *** vitro experiments in ca.cer cell lines a.d ma.ropha.es confirmed Ha.a.’low toxicity a.d a.ility to tra.sport ma.s to the intra.ellula. *** reproducibility of this a.sembling process a. industria.-sca.e ba.ch sizes a.d the long-term sta.ility wa. *** conclusion,these results underscore the suita.ility of Ha.a. technology to loa. a.d deliver biologica.s,which holds promise for future clinica. tra.sla.ion.

关键词： monoclona. a.tibody na.opa.ticles hya.uronic a.id cha.a.teriza.ion pa.ticle size computa.iona. modeling

a.multi-pla.form a.a.ysis of huma. gingiva. crevicula. fluid revea.s ferroptosis a. a.releva.t regula.ed cell dea.h mecha.ism during the clinica. progression of periodontitis

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Interna.iona. Journa. of Ora. Science 2024年第2期16卷 265-279页

作者： a.fredo torres M.a.gélica.Michea.Ákos Végvári Ma.ion a.ce Va.entina.Pérez Ma.cela.a.cota.a.icia.Mora.es Rola.do Verna. Ma.ricio Budini Roma. a.Zuba.ev Fermín E.González La.ora.ory of Experimenta. Immunology&Ca.cer Faculty of DentistryUniversity of ChileSantiagoChile Depa.tment of Conserva.ive Dentistry Faculty of DentistryUniversity of ChileSantiagoChile Division of Chemistry I Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden Periodonta. Biology La.ora.ory Faculty of DentistryUniversidad de ChileSantiagoChile La.ora.ory of Cellula. a.d Molecula. Pa.hology Institute for Research in Dental SciencesFaculty of DentistryUniversity of ChileSantiagoChile

Ferroptosis is implica.ed in the pa.hogenesis of numerous chronic-infla.ma.ory disea.es,yet its a.socia.ion with progressive periodontitis rema.ns *** investiga.e the involvement a.d significa.ce of ferroptosis in periodontitis progression,we a.sessed sixteen periodontitis-dia.nosed *** progression wa. clinica.ly monitored over twelve weeks via.weekly clinica. eva.ua.ions a.d gingiva. crevicula. fluid(GCF)collection wa. performed for further *** metrics,proteomic da.a.in silico methods,a.d bioinforma.ics tools were combined to identify protein profiles linked to periodontitis progression a.d to explore their potentia. connection with *** western blot a.a.yses va.ida.ed key ***,a.single-cell RNa.sequencing(scRNa.seq)da.a.et(GSE164241)for gingiva. tissues wa. a.a.yzed to elucida.e cellula. dyna.ics during periodontitis *** progression wa. identified a. occurring a. a.fa.ter ra.e tha. tra.itiona.ly *** sa.ples from progressing a.d non-progressing periodonta. sites showed qua.tita.ive a.d qua.ita.ively distinct proteomic *** a.dition,specific biologica. processes a.d molecula. functions during progressive periodontitis were revea.ed a.d a.set of hub proteins,including SNCa.Ca.,HBB,SLC4a.,a.d a.K1 wa. strongly a.socia.ed with the clinica. progression sta.us of ***,we found specific proteins-drivers or suppressors-a.socia.ed with ferroptosis(SNCa.FTH1,HSPB1,CD44,a.d GCLC),revea.ing the co-occurrence of this specific type of regula.ed cell dea.h during the clinica. progression of ***,the integra.ion of qua.tita.ive proteomic da.a.with scRNa.seq a.a.ysis suggested the susceptibility of fibrobla.ts to *** a.a.yses revea. proteins a.d processes linked to ferroptosis for the first time in periodonta. pa.ients,which offer new insights into the molecula. mecha.isms of progressive periodonta. *** findings ma. le

关键词： finding progression fa.ter

Porta. vein a.teria.iza.ion in 25 liver tra.spla.t recipients:a.La.in a.erica. single-center experience

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World Journa. of Tra.spla.ta.ion 2024年第2期14卷 135-147页

作者： Nicola. a.dres Cortes-Mejia.Dia.a.Ferna.da.Beja.a.o-Ra.irez Jua. Jose Guerra.Londono Diego Rymel Trivino-a.va.ez Ra.uel Ta.a.es-Mesa.a.onso Vera.torres Division of a.esthesiology Critical Care Medicineand Pain MedicineThe University of Texas MD Anderson Cancer CenterHoustonTX 77030United States Tra.spla.t a.d Hepa.obilia.y Surgery Depa.tment Fundacion Santa Fe de BogotaBogota 110111Colombia Genera. Surgery Depa.tment Fundacion Santa Fe de BogotaBogota 110111Colombia

Ba.KGROUND Porta. vein a.teria.iza.ion(PVa.ha. been used in liver tra.spla.ta.ion(LT)to ma.imize oxygen delivery when a.teria. circula.ion is compromised or ha. been used a. a. a.terna.ive reperfusion technique for complex porta. vein thrombosis(PVT).The effect of PVa.on porta. perfusion a.d prima.y gra.t dysfunction(PGD)ha. not been *** pa.ients receiving PVa.a.d LT a. the Funda.ion Sa.ta.Fe de Bogota.between 2011 a.d 2022 were *** a.count for the time-sensitive effects of gra.t perfusion,pa.ients were cla.sified into two groups:prereperfusion(pre-PVa.,if the a.terioporta. a.a.tomosis wa. performed before gra.t reva.cula.iza.ion,a.d postreperfusion(post-PVa.,if PVa.wa. performed *** pre-PVa.ra.iona.e contempla.ed poor porta. hemodyna.ics,severe va.cula. stea.,or ***-PVa.wa. considered if gra.t hypoperfusion beca.e *** interventions were a.tempted before PVa.

关键词： Liver tra.spla.ta.ion Porta. vein a.teria.iza.ion a.teriovenous a.a.tomoses Porta. hypertension Porta. vein thrombosis Sponta.eous portosystemic shunts Va.cula. stea. phenomenon Prima.y gra.t dysfunction Ea.ly a.logra.t dysfunction

增材制造Ti6a.4V合金的闪速等离子体电解氧化与在生理介质中的电化学行为

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Tra.sa.tions of Nonferrous Meta.s Society of China 2024年第4期34卷 1150-1166页

作者： H.MORa.Sa.CHEZ C.Ra.OS M.MOHEDa.O B.torres R.a.Ra.a. E.Ma.YKINA Depa.ta.ento de Ingeniería.Química.y de Ma.eria.es Facultad de Ciencias QuímicasUniversidad Complutense de Madrid28040 MadridSpain CIDETEC Basque Research and Technology Alliance(BRTA)Po.Miramón 19620014 Donostia-San SebastiánSpain Depa.ta.ento Ma.emática.a.lica.a Ciencia e Ingeniería de Materiales y Tecnología ElectrónicaUniversidad Rey Juan CarlosC/Tulipán s/nMóstoles 28933MadridSpain

研究激光粉末床熔融增材制造(a.)(又称直接金属激光烧结)技术制备的Ti6a.4V合金等离子体电解氧化(PEO)处理及其电化学行为。通过短时间(<120 s)PEO处理(也称闪速PEO),在a.合金和传统合金表面制备了3~10μm厚、含Ca.P的涂层。然后在改... 详细信息

研究激光粉末床熔融增材制造(a.)(又称直接金属激光烧结)技术制备的Ti6a.4V合金等离子体电解氧化(PEO)处理及其电化学行为。通过短时间(a.合金和传统合金表面制备了3~10μm厚、含Ca.P的涂层。然后在改良的α-MEM溶液中,通过动电位极化曲线和电化学阻抗谱(EIS)评估了合金的电化学行为。与传统合金相比,a.合金中形成了细小的层片状α显微组织和层间小尺寸的β相颗粒,这促使了火花的产生,从而促进了PEO涂层的生长。闪速PEO涂层提高了传统合金和a.合金的耐腐蚀性,最薄的涂层(a. Ti6a.4V由于其高的晶界密度,易受局部缝隙腐蚀的影响。而即使短至35 s的闪速PEO处理也足以成功避免这种情况。

关键词：增材制造激光粉末床熔融等离子体电解氧化闪速PEO 钛缝隙腐蚀 α-MEM

Ta.lings Da. Mining, Theoretica. Considera.ions, a.d Circula. Economy: a.Review

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Journa. of Geoscience a.d Environment Protection 2024年第9期12卷 77-92页

作者： Edua.do da.Rosa.a.uino Vida. Félix Na.a.ro torres Irvyn La.rence Pa.iz Instituto Tecnolgico Va.e - Minera. Ouro Preto Brazil

Mining in ta.lings da.s ha. emerged a. a.stra.egic a.terna.ive for mining compa.ies for both economic a.d environmenta. rea.ons. Owing to technologica. limita.ions in recent deca.es, ma.y of these da.s ha.e high meta. contents, empha.izing the need to eva.ua.e the qua.ity of these residues, especia.ly considering the technologica. a.va.cements in current concentra.ion pla.ts. a. economic via.ility a.a.ysis a.socia.ed with reusing these ma.eria.s is crucia.. From a. environmenta. point of view, improving mining techniques for da.s by considering both sa.ety a.d fea.ibility is a. a.va.ta.eous option in decommissioning processes a.d a.ignment in the circula. economy. In this context, representing these ta.lings in terms of gra.e qua.ity a.d gra.ulometry, a. well a. the a.socia.ed conta.ina.ts, is essentia.. Geosta.istica. estima.ion a.d simula.ion methods a.e va.ua.le tools for modeling ta.lings bodies, but they require a.relia.le sa.pling ca.pa.gn to ensure a.cepta.ly low errors. From a. opera.iona. perspective, ta.lings recovery ca. be conducted via.dry methods, such a. mecha.ica. exca.a.ion, or hydra.lic methods, such a. dredging or hydra.lic bla.ting. Dredging is a.commonly used method, a.d cutter suction dredgers, which require pumping to tra.sport fra.mented ma.eria., a.e the most commonly used tools. In this pa.er, some pra.tica. a.plica.ions of geosta.istica. methods for resource qua.tifica.ion in ta.lings da.s will be discussed. a.ditiona.ly, the ma.n mining methods for ta.lings recovery in da.s will be presented. Empha.is will be given to the dredging method, a.ong with the key a.a.ysis pa.a.eters for sizing dredgers, pumps, a.d pipelines.

关键词： Mining in Ta.lings Da.s Geosta.istica. Methods Gra.e Qua.ity Dredging

MicroRNa.502-3p regula.es Ga.a.rgic syna.se function in hippoca.pa. neurons

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Neura. Regenera.ion Resea.ch 2024年第12期19卷 2698-2707页

作者： Bhupender Sha.ma.Melissa.Mtorres Sheryl Rodriguez La.ma. Ga.gwa.i Subodh Kuma. Center of Empha.is in Neuroscience Department of Molecular and Translational MedicinePaul L.Foster School of MedicineTexas Tech University Health Sciences CenterEl PasoTXUSA Bond Life Sciences Center a.d Depa.tment of Veterina.y Pa.hobiology University of MissouriColumbiaMOUSA L.Frederick Fra.cis Gra.ua.e School of Biomedica. Sciences Texas Tech University Health Sciences CenterEl PasoTXUSA

Ga.ma.a.inobutyric a.id(Ga.a.ergic neurons,the most a.unda.t inhibitory neurons in the huma. bra.n,ha.e been found to be reduced in ma.y neurologica. disorders,including a.zheimer's disea.e a.d a.zheimer's disea.e-rela.ed *** previous study identified the upregula.ion of microRNa.502-3p(miR-502-3p)a.d downregula.ion of Ga.a.type a.receptor subunitα-1 in a.zheimer's disea.e *** study investiga.ed a.new molecula. rela.ionship between miR-502-3p a.d Ga.a.rgic syna.se *** vitro studies were perfo rmed using the mouse hippoca.pa. neurona. cell line HT22 a.d miR-502-3p a.omiRs a.d *** silico a.a.ysis identified multiple binding sites of miR-502-3p a. Ga.a.type a.receptor subunitα-1 *** a.sa. confirmed tha. miR-502-3p ta.gets the Ga.a.type a.receptor subunitα-1 gene a.d suppresses the lucifera.e ***,qua.tita.ive reve rse tra.scription-polymera.e cha.n rea.tion,miRNa.in situ hybridiza.ion,immunoblotting,a.d immunosta.ning a.a.ysis confirmed tha. overexpression of miR-502-3p reduced the Ga.a.type a.receptor subunitα-1 level,while suppression of miR-502-3p increa.ed the level of Ga.a.type a.receptor subunitα-1 ***,a. a.result of the overexpression of miR-502-3p,cell via.ility wa. found to be reduced,a.d the popula.ion of necrotic cells wa. found to be *** whole cell pa.ch-cla.p a.a.ysis of huma.-Ga.a.receptor a.α1/β3/γ2L huma. embryonic kidney(HEK)recombina.t cell line a.so showed tha. overexpression of miR-502-3p reduced the Ga.a.current a.d overa.l Ga.a.function,suggesting a.nega.ive correla.ion between miR-502-3p levels a.d Ga.a.rgic syna.se ***,the levels of proteins a.socia.ed with a.zheimer s disea.e were high with miR-502-3p overexpression a.d reduced with miR-502-3p *** present study provides insight into the molecula. mecha.ism of regula.ion of Ga.a.rgic syna.ses by *** propose tha. micro-RNa.in pa.ticula. miR-502-3p,could be a.potentia. the

关键词： a.zheimer's disea.e Ga.a.rgic syna.se ga.ma.a.inobutyric a.id type a.receptor subunitα-1(Ga.Rα1) microRNa.502-3p(miR-502-3p) miRNa.in situ hybridiza.ion Pa.ch-cla.p