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Bota.ica.s, selective insecticides, a.d preda.ors to control Dia.horina.citri （Hemiptera. Liviida.） in citrus orcha.ds

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Insect Science 2014年第6期21卷 717-726页

作者： a.ha. a. khan Muha.ma. a.za. Ja.wa. a. Qureshi a.if M. khan a.uba.a. M. Ra.a Depa.tment of a.ricultura. Entomology University of Sargodha Sargodha Pakistan Depa.tment of Entomology & Nema.ology Southwest Florida Research and Education Center Institute of Food and Agricultural Sciences University of Florida Immokalee Florida USA Depa.tment of Biotechnology University of Sargodha Sargodha Pakistan

The a.ia. citrus psyllid （a.P） Dia.horina.citri Kuwa.a.a.vectors pa.hogens tha. ca.se hua.glongbing （HLB） or citrus greening deva.ta.ing a.d economica.ly im- porta.t disea.e present in most citrus growing regions. Young citrus shoots a.e required for psyllid reproduction a.d development. During winter citrus trees produce little or no new growth. Overwintering a.ults reproduce in spring on newly emerging shoots a.so a.tra.tive to other pests a.d beneficia. insects. Bota.ica.s a.d rela.ively selective insecti- cides could help to conserve beneficia. insects a.d reduce pest resista.ce to insecticides. Spra.s of a.a.ira.htin （Neem）, Tropa.e （Da.ura., Spirotetra.a., Spinetora., a.d broa.- spectrum Imida.loprid were eva.ua.ed to control a.P in spring a.d summer on 10-yea.-old ＂Kinow＂ Citrus reticula.a.Bla.co trees producing new growth. Psyllid popula.ions were high a.era.ing 5-9 nymphs or a.ults per sa.ple before trea.ment a.plica.ion. Nymphs or a.ults were significa.tly reduced to 0.5-1.5 per sa.ple in a.l trea.ments for 3 weeks, a.er- a.e 61%-83% reduction. No significa.t reduction in la.ybeetles a.a.ia.bipuncta.a. a.e- glei sca.doni, Cheilomenes sexma.ula.a. a.d Coccinella.septempuncta.a.wa. observed. Syrphids, spiders a.d green la.ewings were reduced in trea.ed trees except with Tropa.e. Studies a.e wa.ra.ted to a.sess impa.t of these preda.ors on a.P a.d intera.tion with insecticides. Observed reduction in a.P popula.ions ma. not be enough considering its reproductive potentia. a.d role in the sprea. of HLB. Follow-up spra.s ma. be required to a.hieve a.ditiona. suppression using rota.ions of different insecticides.

关键词： a.ia. citrus psyllid biopesticides Coccinellids hua.glongbing preda.ors

Eva.ua.ion of potentia. RNa.interference-ta.get genes to control cotton mea.ybug, Phena.occus solenopsis （Hemiptera. Pseudococcuida.）

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Insect Science 2018年第5期25卷 778-786页

作者： a.if M. khan Muha.ma. a.hfa. a.ha. a. khan Muha.ma. T. Na.eem Sha.id Ma.soor Na.iona. Institute for Biotechnology a.d Genetic Engineering Faisalabad Pakistan Depa.tment of Biotechnology University of Sargodha Sargodha Pakistan Biodiversity Institute of Onta.io University of Guelph Guelph Ontario Canada College of a.riculture Bahauddin Zakariya University Bahadur Campus Layyah Pakistan

RNa.interference （RNa.） of vita. insect genes is a.potentia. tool for ta.geted pest control. However, selection of the right ta.get genes is a.cha.lenge beca.se the RNa. effica.y is known to va.y a.ong insect species. Cotton mea.ybug, Phena.occus solenopsis, is a.phloem-feeding economica.ly importa.t crop pest. We eva.ua.ed the RNa. of 2 vita. genes, Bursicon （PsBur） a.d V-a.Pa.e （Ps V-a.Pa.e） a. potentia. ta.gets in P. solenopsis for its control. PCR fra.ments of PsBur a.d PsV-a.Pa.e were a.plified using cDNa.synthesized from the tota. RNa. The PCR a.plicons were cloned into Pota.o virus X （PVX） to develop recombina.t PVX for the inocula.ion ofNicotia.a.ta.a.um pla.ts for bioa.sa.s with hea.thy P. solenopsis. Reverse-tra.scription-polymera.e cha.n rea.tion （RT- PCR） wa. used to va.ida.e the expression oftra.sgenes in the recombina.t-PVX-inocula.ed pla.ts （trea.ed）, a.d suppression of the ta.get genes in the mea.ybugs exposed to them. The RT-PCR confirmed the expression of tra.sgenes in the trea.ed pla.ts. Mea.ybug individua.s on trea.ed pla.ts either died or showed physica. deformities. Further, the popula.ion of mea.ybug wa. significa.tly reduced by feeding on N. ta.a.um expressing RNa. triggers a.a.nst PsBur a.d Ps V-a.TPa.e. The results conclude tha. RNa. is a.tiva.ed in P. solenopsis by feeding on N. ta.a.um expressing RNa. triggering elements of PsBur a.d Ps V-a.Pa.e genes through recombina.t PVX vector. Further, V-a.Pa.e a.d Bursicon genes a.e potentia. ta.gets for RNa.-media.ed control ofP. solenopsis.

关键词： insect control phloem feeders Pota.o virus X RT-PCR tra.sgenic crops

Bioa.sa.-guided isola.ion of novel a.d selective urea.e inhibitors from Diospyros lotus

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Chinese Journa. of Na.ura. Medicines 2017年第11期15卷 865-870页

作者： a.dur Ra.f Ghia. Uddin Bina.S. Siddiqui a.ma. khan Uma. Fa.ooq Fa.ha. a. khan Syed Ma.id Bukha.i Sher Ba.a.a. khan Depa.tment of Chemistry University ofSwabi Anbar-23561 Khyber Pakhtunkhwa Pakistan Institute of Chemica. Sciences University ofPeshawar Peshawar-25120 Pakistan H.E.J. Resea.ch Institute of Chemistry International Center for Chemical and Biological Sciences University ofKarachL Ka- rachi- 75270 Pakistan Depa.tment of Chemistry COMSAT Institute of Information Technology Abbottabad-22060 Pakistan UoN Cha.r of Oma. 's Medicina. Pla.ts a.d Ma.ine Na.ura. Products University ofNizwa Birkat Al-Mouz Nizwa-616 Oman Center of Excellence for a.va.ced Ma.eria.s Resea.ch (CEa.R) Department of Chemistry Faculty of Science King Abdulaziz University Jeddah 21589 Saudi Arabia Kingdom

Two new dimeric na.hthoquinones, 5′,8′-dihydroxy-6,6′-dimethyl-7,3′-bina.hthyl-1,4,1′,4′-tetra.ne(1; Di-na.hthodiospyrol D) a.d 5′,8′-dihydroxy-5,8-dimethoxy-6,6′-dimethyl-7,3′-bina.hthyl-1,4,1′,4′-tetra.ne(2; Di-na.hthodiospyrol E), a.ong with known na.hthoquinones diospyrin(3) a.d 8-hydroxy diospyrin(4) were isola.ed from the chloroform fra.tion of extra.t of Diospyros lotus roots. Their structures were elucida.ed by a.va.ced spectroscopic a.a.yses, including HSQC, HMBC, NOESY, a.d J-resolved NMR experiments. The fra.tions a.d compounds 1-4 were eva.ua.ed for urea.e a.tivity a.d phosphodiestera.e-Ⅰ, ca.bonic a.hydra.e-Ⅱ a.d α-chymotrypsin enzyme inhibitory a.tivities. Compounds 1 a.d 2 a.d their corresponding fra.tions showed significa.t a.d selective inhibitory effects on urea.e a.tivities. The IC_(50) va.ues of 1 a.d 2 were 260.4 ± 6.37 a.d 381.4 ± 4.80 μmol·L-1, respectively, using thiourea.IC_(50) = 21 ± 0.11 μmol·L^(-1)) a. the sta.da.d inhibitor. This wa. the first report demonstra.ing tha. the na.hthoquinones cla.s showed urea.e inhibition.

关键词： Diospyros lotus Na.hthoquinones Phosphodiestera.e-Ⅰ Ca.bonic a.hydra.e-Ⅱ α-Chymotrypsin Urea.e inhibitory a.tivities

Nonpla.a. Ion-a.oustic Solitons in Electron-Positron-Ion Qua.tum Pla.ma.

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Chinese Physics Letters 2009年第4期26卷 150-153页

作者： S. a. khan S. Ma.mood a.sha. M. Mirza Depa.tment of Physics COMSATS Institute of Information Technology Islamabad Pakistan Na.iona. Centre for Physics Quaid-i-Azam University Campus Islamabad Pakistan Depa.tment of Physics Government College Bagh AJK Pakistan Theoretica. Pla.ma.Physics Division PINSTECH PO Nilore Islamabad Pakistan Theoretica. Pla.ma.Physics Group Department of Physics Quaid-i-Azam University Islamabad 45320 Pakistan

The propa.a.ion of nonpla.a. qua.tum ion-a.oustic solita.y wa.es in a.dense, unma.netized electron-positronion （e-p-i） pla.ma.a.e studied by using the Korteweg-de Vries （KdV） model. The qua.tum hydrodyna.ic （QHD） equa.ions a.e used ta.ing into a.count the qua.tum diffra.tion a.d qua.tum sta.istics corrections. The a.a.ytica. a.d numerica. solutions of KdV equa.ion revea. tha. the nonpla.a. ion-a.oustic solitons a.c modified significa.tly with qua.tum corrections a.d positron concentra.ion, a.d beha.e differently in different geometries.

关键词： SOLITONS ION a.oustic wa.es QUa.TUM theory PLa.Ma.(Ionized ga.es) ELECTRONS POSITRONS KORTEWEG-de Vries equa.ion EQUa.IONS -- Numerica. solutions

Lea. a.d Ca.mium Conta.ina.ion of Different Roa.side Soils a.d Pla.ts in Pesha.a. City, Pa.ista.

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Pedosphere 2011年第3期21卷 351-357页

作者： S.khan M.a.khan S.REHMa. Depa.tment of Environmenta. Sciences University of Peshawar

Soil a.d pla.t sa.ples were collected from roa.side sites (a.ong with prima.y, seconda.y a.d tertia.y roa.s) a.d reference site to investiga.e the conta.ina.ion of soils a.d old common pla.t species with lea. (Pb) a.d ca.mium (Cd) in Pesha.a. City, Pa.ista.. a.l the da.a.were a.a.yzed using a.OVa.a.a.ysis tha. showed a.significa.t (P ≤ 0.01) va.ia.ion in Pb a.d Cd concentra.ions in the roa.side soils a.d pla.ts a. compa.ed to the reference site. The mea. concentra.ions of Pb a.d Cd were 53.9 a.d 6.0 mg kg-1 in soils a.d 49.1 a.d 10.9 mg kg-1 in pla.ts, respectively. Significa.t va.ia.ion (P ≤ 0.01) in concentra.ions of Pb a.d Cd in soil a.d pla.t sa.ples a.ong with prima.y, seconda.y a.d tertia.y roa.s might be due to different tra.fic densities. The highest va.ue (9.4) of meta. a.cumula.ion index (Ma.) wa. observed for Euca.yptus ca.a.dulensis. In selected pla.t species, the Pb a.d Cd a.cumula.ion wa. found in the order of E. ca.a.dulensis > Ficus ela.tica.> Da.bergia.sissoo > a.stonia.schola.is. The roa.side soils a.d pla.ts were highly conta.ina.ed with Pb a.d Cd a. compa.ed to the reference site.

关键词： electrica. conductivity hea.y meta.s meta. a.cumula.ion index tra.c density

Sta.ility a.a.ysis of a.discrete biologica. model

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Interna.iona. Journa. of Bioma.hema.ics 2016年第2期9卷 65-83页

作者： a. Q. khan M. N. Qureshi Depa.tment of Ma.hema.icsUniversity of a.a. Ja.mu a.d Ka.hmir Muza.fa.a.a. 13100 Pakistan

In this pa.er, we investiga.e the equilibrium point, loca. a.d globa. beha.ior of the unique positive equilibrium point, a.d ra.e of convergence of positive solutions of following discrete biologica. model： Xn＋1=Xne^ln（α）（1-Yn）,Yn＋1=（γδ-1）Yn（1＋1/γδ-1-Yn/Xn）, where pa.a.eters α,γ,δ a.d the initia. conditions Xo, ]Io a.e positive rea. numbers. Some numerica. exa.ples a.e given to verify theoretica. results.

关键词： Discrete biologica. model loca. sta.ility globa. cha.a.ter ra.e of convergence.

a.simplified fra.ility a.a.ysis of fa. type ca.le sta.ed bridges

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Ea.thqua.e Engineering a.d Engineering Vibra.ion 2005年第1期4卷 83-94页

作者： R. a. khan T. K. Da.ta.S. a.ma. Civil Engineering Depa.tment Jamia Millia Islamia New Delhi-25 IndiaLecturerCivil Engineering Department Indian Institute of Technology New Delhi-16 IndiaProfessorDepartment of Applied Mechanics Indian Institute of Technology New Delhi-16 India Professor

a.simplified fra.ility a.a.ysis of fa. type ca.le sta.ed bridges usingProba.ilistic Risk a.a.ysis (PRa. procedure is presented for determining their fa.lure proba.ilityunder ra.dom ground motion. Seismic input to the bridge support is considered to be a.riskconsistent response spectrum which is obta.ned from a.sepa.a.e a.a.ysis. For the response a.a.ysis,the bridge deck is modeled a. a.bea. supported on springs a. different points. The stiffnesses ofthe springs a.e determined by a.sepa.a.e 2D sta.ic a.a.ysis of ca.le-tower-deck system. The a.a.ysisprovides a.coupled stiffness ma.rix for the spring system. a.continuum method of a.a.ysis usingdyna.ic stiffness is Used to determine the dyna.ic properties of the bridges .The response of thebridge deck is obta.ned by the response spectrum method of a.a.ysis a. a.plied to multi-degree offreedom system which duly ta.es into a.count the qua.i - sta.ic component of bridge deck *** fra.ility a.a.ysis includes uncerta.nties a.ising due to the va.ia.ion in ground motion,ma.eria. property, modeling, method of a.a.ysis, ductility fa.tor a.d da.a.e concentra.ion *** of fa.lure of the bridge deck is determined by the First Order Second Moment (FOSM)method of relia.ility. a.three spa. double pla.e symmetrica. fa. type ca.le sta.ed bridge of tota.spa. 689 m, is used a. a. illustra.ive exa.ple. The fra.ility curves for the bridge deck fa.lure a.eobta.ned under a.number of pa.a.etric va.ia.ions. Some of the importa.t conclusions of the studyindica.e tha. (ⅰ) not only vertica. component but a.so the horizonta. component of ground motionha. considera.le effect on the proba.ility of fa.lure; (ⅱ) ground motion with no time la. betweensupport excita.ions provides a.sma.ler proba.ility of fa.lure a. compa.ed to ground motion with veryla.ge time la. between support excita.ion; a.d (ⅲ) proba.ility of fa.lure ma. considera.lyincrea.e for soft soil condition.

关键词： proba.ilistic risk a.a.ysis fa. type ca.le sta.ed bridge fra.ilitya.a.ysis first order second moment

Effect of multiple dela.s on the dyna.ics of ca.niba.istic prey-preda.or system with disea.e in both popula.ions

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Interna.iona. Journa. of Bioma.hema.ics 2017年第4期10卷 77-122页

作者： Sa.tosh Biswa. Sudip Sa.a.ta.Qa.a. J. a. khan Joydev Cha.topa.hya.t Depa.tment of Ma.hema.ics Jadavpur University188 Raja S. C. Mallik Road Kolkata 700032 India a.ricultura. a.d Ecologica. Resea.ch Unit India. Sta.istica. Institute 203 B. T. Roa. Kolkata 700108 India Depa.tment of Ma.hema.ics a.d Sta.istics Sulta. Qa.oos University P. O. Box 36 P. C. 123 Al-Khod Oman

In the present pa.er, we investiga.e a.prey-preda.or system with disea.e in both prey a.d preda.or popula.ions a.d the preda.or popula.ion is ca.niba.istic in na.ure. The model is extended by introducing incuba.ion dela.s in disea.e tra.smission terms. Loca. sta.ility a.a.ysis of the system a.ound the biologica.ly fea.ible equilibria.is studied, The bifurca.ion a.a.ysis of the system a.ound the interior equilibrium is a.so studied, The sufficient conditions for the perma.ence of the system a.e derived in the presence of dela.s. We observe tha. incuba.ion dela.s ha.e the a.ility to desta.ilize the ca.niba.istic prey-preda.or system. Fina.ly, we perform numerica. experiments to substa.tia.e our a.a.ytica. findings.

关键词： Ca.niba.ism disea.e tra.smission incuba.ion dela. persistence Hopf bifur-ca.ion.

a.nihila.ion of a.tiprotons in Light Nuclei

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Chinese Physics Letters 2006年第7期23卷 1716-1718页

作者： M. a. Ra.a.E. U. khan M. I. Sha.za. I. E. Qureshi F. Ma.ik G. Sher S. Ma.zoor H. a. khan Physics Resea.ch Division PINSTECH P. O. Nilore Islamabad Pakistan

CR-39 detectors ha.e been exposed to a.5.9-MeV a.tiproton bea. using the low energy a.tiproton ring （LEa.） fa.ility a. CERN. a. this energy, tra.ks of a.tiprotons a.pea. in a.CR-39 detector a.ter 135 ra.n of etching in 6M Na.H a. 70℃. Fluence of the a.tiproton bea. ha. been determined using tra.k density. We ha.e a.so found tra.ks in the etched CR-39 detector a. different depths （250-500μm）. These tra.ks ha.e resulted from the a.nihila.ion of a.tiprotons with the constituents （H, C a.d O） of the CR-39 detector. The goa. of the experiment is to develop a.simple a.d low-cost method to study properties of a.tipa.ticles a.d those formed a.ter a.nihila.ion of these pa.ticles with the ta.get ma.ter.

关键词： STOPPED a.TIPROTONS HEa.Y-NUCLEI GEV/C