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Near-infrared cholangiography with intragallbladder indocyanine green injection in minimally invasive cholecystectomy

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World Journal of Gastrointestinal Surgery 2024年第4期16卷 1017-1029页

作者： Savvas Symeonidis Ioannis Mantzoros Elissavet Anestiadou Orestis ioannidis Panagiotis Christidis Stefanos Bitsianis Vasiliki Bisbinas Konstantinos Zapsalis Trigona Karastergiou Dimitra Athanasiou Stylianos Apostolidis Stamatios Angelopoulos Fourth Department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece ENT Department Royal Cornwall Hospitals NHS TrustCornwall TR13LJUnited Kingdom 1^(st)Propedeutic Surgical Department University Hospital of ThessalonikiAristotle University of ThessalonikiThessaloniki 54636Greece

Laparoscopic cholecystectomy(LC)remains one of the most commonly performed procedures in adult and paediatric *** the advances made in intraoperative biliary anatomy recognition,iatrogenic bile duct injuries during LC represent a fatal complication and consist an economic burden for healthcare systems.A series of methods have been proposed to prevent bile duct injury,among them the use of indocyanine green(ICG)*** most commonly reported method of ICG injection is the intravenous administration,while literature is lacking studies investigating the direct intragallbladder ICG *** narrative mini-review aims to assess the potential applications,usefulness,and limitations of intragallbladder ICG fluorescence in *** screened the available international literature to identify the reports of intragallbladder ICG fluorescence imaging in minimally invasive cholecystectomy,as well as special issues regarding its *** search retrieved four prospective cohort studies,three case-control studies,and one case *** the three case-control studies selected,intragallbladder near-infrared cholangiography(NIRC)was compared with standard LC under white light,with intravenous administration of ICG for NIRC and with standard intraoperative cholangiography(IOC).In total,133 patients reported in the literature have been administered intragallbladder ICG administration for biliary mapping during *** includes several reports of intragallbladder ICG administration,but a standardized technique has not been established *** data suggest that NIRC with intragallbladder ICG injection is a promising method to achieve biliary mapping,overwhelming limitations of IOC including intervention and radiation exposure,as well as the high hepatic parenchyma signal and time interval needed in intravenous ICG ***-based guidelines on the role of intragallbladder ICG fluorescence in LC require the assessment of furth

关键词： Minimally invasive cholecystectomy Laparoscopic cholecystectomy Biliary tract mapping Indocyanine green Near-infrared fluorescent cholangiography Intracystic indocyanine green Intragallbladder indocyanine green Bile duct injury

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Experimental models of high-risk bowel anastomosis in rats:A systematic review

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World Journal of Experimental Medicine 2024年第2期14卷 114-131页

作者： Georgios Ntampakis Manousos-Georgios Pramateftakis Elissavet Anestiadou Stefanos Bitsianis Orestis ioannidis Chryssa Bekiari George Koliakos Maria Karakota Anastasia Tsakona Angeliki Cheva Stamatios Angelopoulos Fourth Department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece Laboratory of Anatomy and Histology School of Veterinary MedicineFaculty of Health SciencesAristotle University of ThessalonikiThessaloniki 54124Greece Experimental and Research Center Papageorgiou General Hospital of ThessalonikiThessaloniki 56403Greece Laboratory of Biochemistry Faculty of MedicineAristotle University of ThessalonikiThessaloniki 54124Greece Department of Pathology Faculty of MedicineAristotle University of ThessalonikiThessaloniki 54124Greece

BACKGROUND Anastomotic leaks remain one of the most dreaded complications in gastrointestinal surgery causing significant morbidity,that negatively affect the patients’quality of *** studies play an important role in understanding the pathophysiological background of anastomotic healing and there are still many fields that require further *** drawn from these studies can lead to interventions or techniques that can reduce the risk of anastomotic leak in patients with high-risk *** the advances in experimental protocols and techniques,designing a high-quality study is still challenging for the investigators as there is a plethora of different models *** To review current state of the art for experimental protocols in high-risk anastomosis in *** This systematic review was performed according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses *** identify eligible studies,a comprehensive literature search was performed in the electronic databases PubMed(MEDLINE)and Scopus,covering the period from conception until 18 October *** From our search strategy 102 studies were included and were categorized based on the mechanism used to create a high-risk *** of assessing anastomotic healing were extracted and were individually *** Anastomotic healing studies have evolved over the last decades,but the findings are yet to be translated into human *** is a need for high-quality,well-designed studies that will help to the better understanding of the pathophysiology of anastomotic healing and the effects of various interventions.

关键词： High-risk anastomosis Rats Experimental models Bowel Colon Anastomotic leak Colon resection Inflammatory bowel disease Intra-abdominal sepsis Bursting pressure

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Effect of growth hormone on colonic anastomosis after intraperitoneal administration of 5-fluorouracil, bleomycin and cisplatin: An experimental study

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2679-2688页

作者： Ioannis Lambrou Ioannis Mantzoros Orestis ioannidis Dimitrios Tatsis Elissavet Anestiadou Vasiliki Bisbinas Manousos-Georgios Pramateftakis Efstathios Kotidis Barbara Driagka Ourania Kerasidou Savvas Symeonidis Stefanos Bitsianis Freideriki Sifaki Konstantinos Angelopoulos Haralabos Demetriades Stamatios Angelopoulos Fourth Department of Surgery Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece Department of ENT Royal Cornwall Hospitals NHS TrustCornwall TR13LJUnited Kingdom

BACKGROUND Growth hormone(GH)plays a crucial role in wound healing and tissue repair in postoperative *** particular,colonic anastomosis healing following colorectal surgery is impaired by numerous chemotherapy *** To investigate whether GH can improve the healing of a colonic anastomosis following the adverse effects of intraperitoneal administration of 5-fluorouracil(5-FU),bleomycin and *** Eighty Wistar rats underwent laparotomy and a 1 cm-resection of the transverse colon,followed by an end-to-end anastomosis under general *** rats were blindly allocated into four equal groups and administered a different daily intraperitoneal therapeutic regimen for 6 *** control group(A)received normal *** B received chemotherapy with 5-FU(20 mg/kg),bleomycin(4 mg/kg)and cisplatin(0.7 mg/kg).Group C received GH(2 mg/kg),and group D received the aforementioned combination chemotherapy and GH,as *** rats were sacrificed on the 7th postoperative day and the anastomoses were macroscopically and microscop-ically *** weight,bursting pressure,hydroxyproline levels and inflammation markers were *** All rats survived until the day of sacrifice,with no infections or other complications.A decrease in the body weight of group D rats was observed,not statistically significant compared to group A(P=1),but significantly different to groups C(P=0.001)and B(P<0.01).Anastomotic dehiscence rate was not statistically different between the *** pressure was not significantly different between groups A and D(P=1.0),whereas group B had a significantly lower bursting pressure compared to group D(P<0.001).All groups had significantly more adhesions than group ***,as a measurement of collagen deposition,was significantly higher in group D compared to group B(P<0.05),and higher,but not statistically significant,compared to group *** changes in group D were recorded,compared to group A

关键词： Growth hormone Colonic anastomosis Adhesion Bursting pressure Collagen Hydroxyproline Inflammation Neoangiogenesis Chemotherapy

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Wolbachia symbiosis and insect immune response

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Insect Science 2008年第1期15卷 89-100页

作者： Stefanos Siozios Panagiotis Sapountzis Panagiotis ioannidis Kostas Bourtzis Department of Environmental and Natural Resources Management University of Ioannina Agrinio 30100 Greece

Bacterial intraceUular symbiosis is very common in insects, having significant consequences in promoting the evolution of life and biodiversity. The bacterial group that has recently attracted particular attention is Wolbachia pipientis which probably represents the most ubiquitous endosymbiont on the planet. W. pipientis is a Gram-negative obligatory intracellular and maternally transmitted a-proteobacterium, that is able to establish symbiotic associations with arthropods and nematodes. In arthropods, Wolbachia pipientis infections have been described in Arachnida, in Isopoda and mainly in Insecta. They have been reported in almost all major insect orders including Diptera, Coleoptera, Hemiptera, Hymenoptera, Orthoptera and Lepidoptera. To enhance its transmission, W. pipientis can manipulate host reproduction by inducing parthenogenesis, feminization, male killing and cytoplasmic incompatibility. Several polymerase chain reaction surveys have indicated that up to 70% of all insect species may be infected with W. pipientis. How does W. pipientis manage to get established in diverse insect host species？ How is this intracellular bacterial symbiont species so successful in escaping the host immune response？ The present review presents recent advances and ongoing scientific efforts in the field. The current body of knowledge in the field is summarized, revelations from the available genomic information are presented and as yet unanswered questions are discussed in an attempt to present a comprehensive picture of the unique ability of W. pipientis to establish symbiosis and to manipulate reproduction while evading the host＇s immune system.

关键词： Drosophila immune response insects symbionts Wolbachia

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Atypical anastomotic malignancies of small bowel after subtotal gastrectomy with BillorthⅡgastroenterostomy for peptic ulcer:Report of three cases and review of the literature

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World Journal of Gastrointestinal Oncology 2018年第7期10卷 194-201页

作者： Efstathios Kotidis Orestis ioannidis Manousos George Pramateftakis Konstantinos Christou Ioannis Kanellos Konstantinos Tsalis Fourth Surgical Department Medical SchoolAristotle University of ThessalonikiThessaloniki 57010Greece

AIM To present patients who developed small-bowel malignancy at the level of the gastrointestinal anastomosis decades after a subtotal gastrectomy for ulcer,to review relevant literature,and to attempt to interpret the reasons those cancers developed to these postsurgical non-gastric *** For the current retrospective study and review of literature,the surgical and histopathological records dated from January 1,1993 to December 31,2017 of our department were examined,searching for patients who have undergone surgical treatment of small-bowel malignancy to identify those who have undergone subtotal gastrectomy for benign peptic ulcer.A systematic literature search was also conducted using Pub Med,EMBASE,and Cochrane Library to identify similar *** We identified three patients who had developed smallintestine malignancy at the level of the gastrointestinal anastomosis decades after a subtotal gastrectomy with Billroth Ⅱ gastroenterostomy for benign peptic ulcertwo patients with adenocarcinoma originated in the Braun anastomosis and one patient with lymphoma of the efferent *** three patients were submitted to surgical resection of the tumor with Roux-en-Y reconstruction of the digestive *** the literature review,we only found one case of primary small-intestinal cancer that originated in the efferent loop after Billroth Ⅱ gastrectomy because of duodenal ulcer but none reporting Braun anastomosis adenocarcinoma following partial gastrectomy for benign *** also did not find any case of efferent loop lymphoma following *** Anastomotic gastric cancer following distal gastrectomy for peptic ulcer is a well-established clinical ***,malignancies of the afferent or efferent loop of the gastrointestinal anastomosis are extremely *** substantial diversion of the potent carcinogenic pancreaticobiliary secretions through the Braun anastomosis and the stomach hypochlorhydria,allowing the formation of carci

关键词： Anastomotic cancer Efferent loop Braun anastomosis Adenocarcinoma Anaplastic large cell lymphoma

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Non-albicans Candida prosthetic joint infections: A systematic review of treatment

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World Journal of Clinical Cases 2019年第12期7卷 1430-1443页

作者： Christos Koutserimpas Stylianos G Zervakis Sofia Maraki Kalliopi Alpantaki Argyrios ioannidis Diamantis P Kofteridis George Samonis Department of Orthopaedics and Traumatology “251” Hellenic Air Force General Hospital of Athens Athens 11525 Greece Department of Internal Medicine University Hospital of Heraklion Crete Heraklion 71110 Greece Department of Clinical Microbiology and Microbial Pathogenesis University Hospital of Heraklion Crete Heraklion 71110 Greece Department of Materials Science and Technology University of Crete Heraklion Heraklion 71110 Greece Department of General Surgery “Sismanoglion” General Hospital of Athens Athens 15126 Greece

BACKGROUND Non-albicans Candida prosthetic joint infections (PJIs) are rare. Optimal treatment involves a two-stage revision surgery in combination with an antifungal agent. However, no clear guidelines have been developed regarding the agent or treatment duration. Hence, a broad range of antifungal and surgical treatments have been reported so far. AIM To clarify treatment of non-albicans Candida PJIs. METHODS A literature review of all existing non-albicans Candida PJIs cases through April 2018 was conducted. Information was extracted about demographics, comorbidities, responsible species, duration and type of antifungal treatment, type of surgical treatment, time between initial arthroplasty and symptom onset, time between symptom onset and definite diagnosis, outcome of the infection and follow-up. RESULTS A total of 83 cases, with a mean age of 66.3 years, were located. The causative yeast isolated in most cases was C. parapsilosis (45 cases;54.2%), followed by C. glabrata (18 cases;21.7%). The mean Charlson comorbidity index was 4.4 ± 1.5. The mean time from arthropalsty to symptom onset was 27.2 ± 43 mo, while the mean time from symptom onset to culture-confirmed diagnosis was 7.5 ± 12.5 mo. A two stage revision arthroplasty (TSRA), when compared to one stage revision arthroplasty, had a higher success rate (96% vs 73%, P = 0.023). Fluconazole was the preferred antifungal agent (59;71%), followed by amphotericin B (41;49.4%). CONCLUSION The combination of TSRA and administration of prolonged antifungal therapy after initial resection arthroplasty is suggested on the basis of limited data.

关键词： Fungal prosthetic joint infection Knee arthroplasty infection Hip arthroplasty infection Antifungal treatment Non-albicans Candida prosthetic joint infections

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A framework for inspection of dies attachment on PCB utilizing machine learning techniques

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Journal of Management Analytics 2018年第2期5卷 81-94页

作者： Thanasis Vafeiadis Nikolaos Dimitriou Dimosthenis ioannidis Tracy Wotherspoon Gregory Tinker Dimitrios Tzovaras Information Technologies Institute(ITI) CERTHThessalonikiGreece MICROSEMI Semiconductor Limited GwentUK

Decision Support Systems are considered as a robust technology able to provide an advantage to several manufacturing *** part of the Z-Fact0r EU project Early Stage-Decision Support System,a framework for the inspection of a printed circuit boards(PCB)and the inference of faults,regarding the excess or insufficient glue,is *** the inspection of the PCB,a pixel-based vector of the regions of interest is utilized and several very popular in research community machine learning algorithms are tested on their performance on fault *** order to determine the most efficient and effective classifier,a schema of Monte Carlo simulations for each classification algorithm and set of hyper-parameters was *** results show a superiority of the support vector machine(SVM)classifier with polynomial and radial basis function kernels,compared to the *** best overall classifier was the SVM polynomial(accuracy:81.39%,f-measure:78.72%).

关键词： PCB image processing feature extraction machine learning Monte Carlo

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Midgut neuroendocrine tumor presenting with acute intestinal ischemia

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World Journal of Gastroenterology 2017年第45期23卷 8090-8096页

作者： Ioannis Mantzoros Natalia Antigoni Savvala Orestis ioannidis Styliani Parpoudi Lydia Loutzidou Despoina Kyriakidou Angeliki Cheva Vasileios Intzos Konstantinos Tsalis Fourth Surgical Department Faculty of Health ScienceSchool of MedicineAristotle Uni-versity of Thessaloniki“G.Papanikolaou”General Hospital Department of Pathology General Hospital“G.Papanikolaou” Department of Radiology General Hospital“G.Papanikolaou”

Neuroendocrine tumors represent a heterogeneous group of neoplasms that arise from neuroendocrine cells and secrete various peptides and bioamines. While gastrointestinal neuroendocrine tumors,commonly called carcinoids,account for about 2/3 of all neuroendocrine tumors,they are relatively rare. Small intestine neuroendocrine tumors originate from intestinal enterochromaffin cells and represent about 1/4 of small intestine neoplasms. They can be asymptomatic or cause nonspecific symptoms,which usually leads to a delayed diagnosis. Imaging modalities can aid diagnosis and surgery remains the mainstay of treatment. We present a case of a jejunal neuroendocrine tumor that caused nonspecific symptoms for about 1 year before manifesting with acute mesenteric ischemia. Abdominal X-rays revealed pneumatosis intestinalis and an abdominal ultrasound and computed tomography confirmed the diagnosis. The patient was submitted to segmental enterectomy. Histopathological study demonstrated a neuroendocrine tumor with perineural and arterial infiltration and lymph node metastasis. The postoperative course was uneventful and the patient denied any adjuvant treatment.

关键词： Small intestine Carcinoid enterochromaffin cells jejunum enterectomy Pneumatosis intestinalis

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Is Public Co-Ordination of Investment in Information Security Desirable?

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Journal of Information Security 2016年第2期7卷 60-80页

作者： Christos ioannidis David Pym Julian Williams Department of Economics University of Bath Bath UK Department of Computer Science University College London London UK Durham University Business School Durham UK

This paper provides for the presentation, in an integrated manner, of a sequence of results addressing the consequences of the presence of an information steward in an ecosystem under attack and establishes the appropriate defensive investment responses, thus allowing for a cohesive understanding of the nature of the information steward in a variety of attack contexts. We determine the level of investment in information security and attacking intensity when agents react in a non-coordinated manner and compare them to the case of the system’s coordinated response undertaken under the guidance of a steward. We show that only in the most well-designed institutional set-up the presence of the well-informed steward provides for an increase of the system’s resilience to attacks. In the case in which both the information available to the steward and its policy instruments are curtailed, coordinated policy responses yield no additional benefits to individual agents and in some case they actually compared unfavourably to atomistic responses. The system’s sustainability does improve in the presence of a steward, which deters attackers and reduces the numbers and intensity of attacks. In most cases, the resulting investment expenditure undertaken by the agents in the ecosystem exceeds its Pareto efficient magnitude.

关键词： Information Security Information Stewardship Investment Public Co-Ordination

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Effects of albumin/glutaraldehyde glue on healing of colonic anastomosis in rats

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World Journal of Gastroenterology 2017年第31期23卷 5680-5691页

作者： Kalliopi Despoudi Ioannis Mantzoros Orestis ioannidis Aggeliki Cheva Nikolaos Antoniou Dimitrios Konstantaras Savvas Symeonidis Manousos George Pramateftakis Efstathios Kotidis Stamatis Angelopoulos Konstantinos Tsalis Fourth Surgical Department Medical SchoolAristotle University of Thessaloniki Department of Pathology General Hospital“G.Papanikolaou”57010 ThessalonikiGreece

To evaluate the effect of local surgical adhesive glue (albumin/glutaraldehyde-Bioglue) on the healing of colonic anastomoses in rats. METHODSForty Albino-Wistar male rats were randomly divided into two groups, with two subgroups of ten animals each. In the control group, an end-to-end colonic anastomosis was performed after segmental resection. In the Bioglue group, the anastomosis was protected with extraluminar application of adhesive glue containing albumin and glutaraldehyde. Half of the rats were sacrificed on the fourth and the rest on the eighth postoperative day. Anastomoses were resected and macroscopically examined. Bursting pressures were calculated and histological features were graded. Other parameters of healing, such as hydroxyproline and collagenase concentrations, were evaluated. The experimental data were summarized and computed from the results of a one-way ANOVA. Fisher’s exact test was applied to compare percentages. RESULTSBursting pressures, adhesion formation, inflammatory cell infiltration, and collagen deposition were significantly higher on the fourth postoperative day in the albumin/glutaraldehyde group than in the control group. Furthermore, albumin/glutaraldehyde significantly increased adhesion formation, inflammatory cell infiltration, neoangiogenesis, and collagen deposition on the eighth postoperative day. There was no difference in fibroblast activity or hydroxyproline and collagenase concentrations. CONCLUSIONAlbumin/glutaraldehyde, when applied on colonic anastomoses, promotes their healing in rats. Therefore, the application of protective local agents in colonic anastomoses leads to better outcomes.

关键词： Adhesions Bursting pressure Bioglue™ Albumin/glutaraldehyde Collagen Colonic anastomosis

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