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High-resolution genome-wide association study of a large Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

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Horticulture Research 2023年第1期10卷 113-127页

作者： Kateřina Holušová JanaČmejlová Pavol Suran RadekČmejla JiříSedlák Lubor Zelený Jan Bartoš Centre of the Region Hanáfor Biotechnological and Agricultural Research Institute of Experimental Botany of the Czech Academy of Sciencesšlechtitelu 31Olomouc77900Czech Republic Research and Breeding Institute of Pomology Holovousy Ltd. Holovousy 129Holovousy50801Czech Republic

In sweet cherry(Prunus avium L.),quantitative trait loci have been identified for fruit maturity,colour,firmness,and size to develop markers for marker-assisted ***,resolution is usually too low in those analyses to directly target candidate genes,and some associations are *** contrast,genome-wide association studies are performed on broad collections of accessions,and assemblies of reference sequences from Tieton and Satonishiki cultivars enable identification of single nucleotide polymorphisms after whole-genome sequencing,providing high marker *** hundred and thirty-five sweet cherry accessions were sequenced and phenotyped for harvest time and fruit colour,firmness,and ***-wide association studies were used to identify single nucleotide polymorphisms associated with each trait,which were verified in breeding material consisting of 64 additional accessions.A total of 1767106 single nucleotide polymorphisms were *** that density,significant single nucleotide polymorphisms could be linked to co-inherited haplotype blocks(median size∼10 kb).Thus,markers were tightly associated with respective phenotypes,and individual allelic combinations of particular single nucleotide polymorphisms provided links to distinct *** addition,yellow-fruit accessions were sequenced,and a∼90-kb-deletion on chromosome 3 that included five MYB10 transcription factors was associated with the ***,the study confirmed numerous quantitative trait loci from bi-parental populations using high-diversity accession populations,identified novel associations,and genome-wide association studies reduced the size of trait-associated loci from megabases to kilobases and to a few candidate genes per ***,a framework is provided to develop molecular markers and evaluate and characterize genes underlying important agronomic traits.

关键词： fir breeding maturity

Vertex-antimagic Labelings of Regular Graphs

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Acta Mathematica Sinica,english Series 2012年第9期28卷 1865-1874页

作者： Ali AHMAD Kashif ALI Martin BACA Petr KovAR Andrea SeMANICovA-FeNovCíKovA College of Computer Science and Information Systems Jazan University Faculty of Mathematics COMSATS Institute of Information Technology Department of Applied Mathematics and Informatics Technical University Department of Applied Mathematics VB-Technical University of Ostrava

Let G = （V, e） be a finite, simple and undirected graph with p vertices and q edges. An （a, d）-vertex-antimagic total labeling of G is a bijection f from V（G） t2 e（G） onto the set of consecutive integers 1, 2,... ,p ＋ q, such that the vertex-weights form an arithmetic progression with the initial term a and difference d, where the vertex-weight of x is the sum of the value f（x） assigned to the vertex x together with all values f（xy） assigned to edges xy incident to x. Such labeling is called super if the smallest possible labels appear on the vertices. In this paper, we study the properties of such labelings and examine their existence for 2r-regular graphs when the difference d is 0, 1,..., r ＋ 1.

关键词： Super vertex-antimagic total labeling vertex-antimagic edge labeling regular graph

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精工大器

中国服装（北京） 2013年第16期 52-53页

作者： 207ers

在小前锋的位置上，将精准的投篮、灵活的突破与出色的篮球意识集于一身的杜兰特就像一部在场e稳定运转的得分机器，精密、准确而高效，而这电启发了杜兰特系列设计总监张传禧（LeOCHANG）。他从杜兰特在场上准确到位的移动联想到了高... 详细信息

在小前锋的位置上，将精准的投篮、灵活的突破与出色的篮球意识集于一身的杜兰特就像一部在场e稳定运转的得分机器，精密、准确而高效，而这电启发了杜兰特系列设计总监张传禧（LeOCHANG）。他从杜兰特在场上准确到位的移动联想到了高级腕表的精密工艺，由此打造—款融合精准眭能与精巧外观的新概念战靴，便成了他为“杜兰特6”设定的目标。

关键词：稳定运转精密工艺机器腕表

相见不如怀念—记或已“失传”的篮球鞋科技（连载三）PeeBOK篇

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中国服装（北京） 2009年第6期 38-41页

作者： 207ers

1989年在ReeBOK发展历史中注定是不平凡的一年．不仅因为划时代的PUMP技术在这一年诞生，还在于ReeBOK推出了自己的首个缓震技术eNeRGYReTURN SYSTeM（简称e.R.S.）。这种名为“e.R.S.能量回复系统”的技术由世界著名的树脂材料供应商与... 详细信息

1989年在ReeBOK发展历史中注定是不平凡的一年．不仅因为划时代的PUMP技术在这一年诞生，还在于ReeBOK推出了自己的首个缓震技术eNeRGYReTURN SYSTeM（简称e.R.S.）。这种名为“e.R.S.能量回复系统”的技术由世界著名的树脂材料供应商与ReeBOK共同开发，它是一种管状的热塑聚氯酯弹性材料．将这种材料置于中底可以利用其自身的离弹特性．吸收运动中产生的冲击力，并将这种能量迅速转化为动能，从而降低运动者的受伤几率。

关键词：篮球鞋科技树脂材料 PUMP 发展历史弹性材料技术供应商

用于伏尔加河地区矮秆春小麦育种的Rht基因的研究和选择

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华中农业大学学报 2000年第3期19卷 209-212页

作者： Loba.,YV Kurp.,ov 俄罗斯萨拉托夫州立农业大学生物工程与植物遗传育种系俄罗斯萨拉托夫州东南地区农科所

对小麦品系Ｓａｒａｔｏｖｓｋａｙａ２９的不同Ｒｈｔ等位的一套近等基因系进行了田间试验，以测定６个矮杆基因的育种值。在不灌溉、灌溉并施用不同剂量的肥料（Ｎ１２０，Ｐ１００，Ｋ６０和Ｎ１８０，Ｐ１６０，Ｋ１００）三种条件... 详细信息

对小麦品系Ｓａｒａｔｏｖｓｋａｙａ２９的不同Ｒｈｔ等位的一套近等基因系进行了田间试验，以测定６个矮杆基因的育种值。在不灌溉、灌溉并施用不同剂量的肥料（Ｎ１２０，Ｐ１００，Ｋ６０和Ｎ１８０，Ｐ１６０，Ｋ１００）三种条件下测定Ｒｈｔ基因对株高、产量及产量构成因子、品质的影响。结果表明，Ｒｈｔ基因可以用于伏尔加河地区条件下魏杆小麦品系的选育。

关键词：春小麦产量品质矮杆基因育种值

Sc元素添加对双辊铸轧Al−Cu−Li−Mg−Zr基合金热处理的影响

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1759-1773页

作者： Rostislav KRÁLÍK Barbora KŘIVSKÁ Lucia BAJTOŠovÁ Mykhailo STOLBCHeNKO Mirko SCHAPeR Olexandr GRYDIN Miroslav CIeSLAR Department of Physics of Materials Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116PragueCzech Republic Department of Materials Science Faculty of Mechanical EngineeringUniversity of PaderbornWarburger Straße 10033098PaderbornGermany

提出制备含Sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金... 详细信息

提出制备含Sc的Al−Cu−Li−Mg−Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg−Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金均先在300℃下退火30 min,然后在450℃下退火30 min以及530℃下退火30 min,随后进行室温水淬。前两段退火后组织中生成了细小、弥散的Al_(3)Zr/Al_(3)(Sc,Zr)颗粒。最后的退火步骤起到了均匀化/固溶处理的作用。双辊铸轧材料的细小晶粒结构使含有主要合金元素的原始颗粒在较短的退火时间内溶解。缩短保温时间可以限制表面层中Li的耗尽和Al_(3)Zr/Al_(3)(Sc,Zr)弥散相的明显粗化。铸态含Sc材料已经具有明显细小的晶粒结构,而经均匀化/固溶处理后生成的Al_(3)(Sc,Zr)弥散相抑制了晶粒在高温下发生粗化。在后续180℃下时效处理后,形成了θʹ(Al_(2)Cu)、T1(Al_(2)CuLi)和S'(Al_(2)CuMg)强化相。在峰值时效状态下,屈服强度提高了200 MPa,这一数值与类似直接冷却铸造材料的相当,从而证实了所提方案的合理性。

关键词：显微组织力学性能 Al−Cu−Li基合金 Sc合金化双辊铸轧

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Virome identification in wheat in the Czech Republic using small RNA deep sequencing

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Journal of Integrative Agriculture 2020年第7期19卷 1825-1833页

作者： Khushwant SINGH Jana JAROSovA Jan FOUSeK CHeN Huan Jiban Kumar KUNDU Plant Virus and Vector Interactions Group Division of Crop Protection and Plant HeatthCrop Research InstitutePrague 16106Czech Republic Zhejiang Tianke High-Technology Development Co. Ltd.Hangzhou 310012P.R.China

High-throughput deep-sequencing technology and bioinformatics analysis of the small RNA(sRNA)population isolated from plants allows universal virus detection and complete virome reconstruction for a given *** the present sRNA deep-sequencing analysis of virus-infected wheat samples in the Czech Republic,samples were firstly tested for barley yellow dwarf viruses(BYDVs),wheat streak mosaic virus(WSMV)and wheat dwarf virus(WDV)using eLISA,RT-PCR and *** sRNA sequencing of these samples yielded more than^60 million single-end 50-bp reads with high confidence for nine field samples of ***,16.5%of reads were virus-specific and 83.5%were mapped to the *** 21-nt reads(-7.7e+06 reads)were found than 24-nt(~6.20e+06 reads)or 22-nt(-4.30e+06 reads)*** novo assembly of the high-quality contigs revealed the presence of three earlier repoted viruses in the Czech Republic:BYDVs(31.48%),WSMV(24.23%)and WDV(2666%).We also showed the presence of cereal yellow dwarf virus(14.33%;two species CYDV-RPS and CYDV-RPV family Luteoviridae/Polerovirus)and wheat yellow dwarf virus(WYDV,3.30%;Luteoviridae).Phylogenetic analysis showed CYDV and WYDV grouped separately from ***,several recombination breakpoints were found among the groups of yellow dwarf viruses(BYDVs,CYDV,and WYDV).Using RNA deep sequencing,we confirmed the presence of the three known viruses(BYDVs,WSMV,and WDV)and the first record of two species of CYDV and WYDV in wheat in the Czech Republic.

关键词： NGS sRNA BYDVs CYDV WSMV WYDV WDV

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饲料中牛特异性DNA的鉴定

环境卫生学杂志 2001年第3期 189-190页

作者： Kr mái Ｐ Ren ová e 王勇牛胜田

A stepwise optimization strategy to formulate in situ gelling formulations comprising fluconazole-hydroxypropyl-beta-cyclodextrin complex loaded niosomal vesicles and eudragit nanoparticles for enhanced antifungal activity and prolonged ocular delivery

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Asian Journal of Pharmaceutical Sciences 2020年第5期15卷 617-636页

作者： Heba elmotasem Ghada e.A.Awad Pharmaceutical Technology Department National Research CentreCairo 12622Egypt Chemistry of Natural and Microbial Product Department National Research CentreCairo 12622Egypt

Fungal keratitis and endopthalmitis are serious eye ***(FL)is indicated for their treatment,but suffers from poor topical ocular *** study was intended to improve and prolong its ocular *** niosomal vesicles were prepared using span ***,polymeric nanoparticles were prepared using cationic eudragit RS100 and eudragit *** investigated particles had adequate entrapment efficiency(ee%),nanoscale particle size and high zeta ***,formulations were optimized using full factorial ***-HP-β-CD complex was encapsulated in selected eudragit nanoprticles(FL-CD-ers1)and niosmal *** niosomes were further coated with cationic and bioadhesive chitosan(FL-CD-Nios-ch).ee%for FL-CD-ers1 and FL-CD-Niosch formulations were 76.4%and 61.7%;particle sizes were 151.1 and 392 nm;also,they exhibited satisfactory zeta potential+40.1 and+28.5 m *** situ gels were prepared by poloxamer P407,HPMC and chitosan and evaluated for gelling capacity,rheological behavior and gelling *** increase the precorneal residence time,free drug and selected nano-formulations were incorporated in the selected in situ *** study revealed sustained release within 24 *** through excised rabbits corneas demonstrated enhanced drug flux and large AUC0-6 h in comparison to plain *** permeation of selected formulations labeled with Rhodamine B was visualized by Confocal laser *** study and in vivo tolerance test evidenced *** vivo susceptibility test using Candida albicans depicted enhanced growth inhibition and sustained *** this study the adopted stepwise optimization strategy combined cylodextrin complexation,drug nano-encapsulation and loading within thermosenstive in situ ***,the developed innovated formulations displayed boosted corneal permeation,enhanced antifungal activity and prolonged action.

关键词： Fluconazole Ocular eudragit nanoparticles Cyclodextrin Niosomes In situ gel