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World Journal of Gastroenterology 2001年第5期7卷 678-684页

作者： An-Chun Cheng Ming-Shu Wang Xiao-Yue Chen Yu-Fei Guo Zhao-Yu Liu peng-Fei Fang College of animal Science and Veterinary Medicine,sichuan Agricultural University,Xinkang road,Yaan City 625014,sichuan province,china College of animal Science and Veterinary Medicine Sichuan Agricultural University Yaan 625014 Sichuan Province China. Chenganchun@***

AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV),the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in china, as well as the pathomorphological development in goslings infected artificially with NGVEV. METHODS: (1)NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration chromatography, and SDS-pAGE. (2)Forty-2-day-old White sichuan goslings were orally administered with NGVEV and 24 hr later 2 birds were randomly selected and killed at 24hr intervals until death occurred. Specimens(duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light microscope. rESULTS: NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15 polypeptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 days postinfection artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudo-membrane or of the mixture of fibrous exudate and necrotic intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen.

关键词： Adenoviridae animals Brain china DNA, Viral Enteritis Female Geese Inclusion Bodies, Viral poultry diseases research Support, Non-U.S. Gov't Virulence Viscera

Effect of cholecystokinin octapeptide on diacylglyceroI-pKC signaling pathway in rat pulmonary interstitial macrophages stimulated by lipopolysaccharide

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Acta pharmacologica Sinica 2005年第12期26卷 1497-1504页

作者： Shun-jiang XU~2 Wei-juan GAO Bin CONG~3 Chun-ling MA Shu-jin LI Yi-ling LING Zhen-yong GU Yu-xia YAO Department of pathophysiology,Hebei Medical University,Shijiazhuang 050017,china the Hebei province center for disease prevention and control,Shijiazhuang 050021,china. Department of pathophysiology Hebei Medical University Shijiazhuang China

Aim:To investigate the effect of cholecystokinin octapeptide(CCK-8)on thediacylglycerol-protein kinase C(DAG-pKC)signaling pathway in rat pulmonaryinterstitial macrophages(pIM)stimulated by lipopolysaccaride(LpS).Methods:The pIM from rat lung tissues were isolated using the collagenase digestionmethod combined with alveolar lavage and pulmonary vessel *** and pKC activity were measured by radioenzymatic *** transloca-tion of pKCζ was determined by semi-quantitative immunoblot ***:CCK-8,at high concentrations(1×10-6-1×10-5mol/L),decreased DAG content andinhibited pKC activity and pKCζ translocation compared with that in rat restingpIM of a control group(ppS increased DAG content,and promoted pKCactivity and pKC~ translocation(p8 decreased LpS-induced DAGcontent and inhibited LpS-induced pKC activity and pKCζ translocation signifi-cantly at 1×10-8-1×10-5mol/L(pry effect of CCK-8 could beabrogated partly by proglumide(non-selective CCK receptor antagonist),Cr-1409(selective CCK-A receptor antagonist),and Cr-2945(selective CCK-Breceptor antagonist)in a concentration-dependent manner(p8 was a negative modulator of the DAG-pKC signaling pathway in rat restingpIM,which is very important for maintaining body *** significantlyinhibited LpS-induced DAG content,pKC activity and pKCζ translocation in aconcentration-dependent *** CCK receptor,especially the CCK-Areceptor,might play a major role in this process.

关键词： cholecystokinin protein kinase C translocation lung macrophages lipopolysaccharides

Effects of topiramate and carbamazepine on thyroid hormone level in adults with epilepsy

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Neural regeneration research 2006年第8期1卷 706-709页

作者： Liang Yu, Yulan Huang, Hongbin Sun, Jie Liu, Fei Xu, Xiaoping Wang sichuan Academy of Medical Sciences Department of Neurology, sichuan provincial people’s Hospital, chengdu 610072, sichuan province, china sichuan Academy of Medical Sciences Department of Neurology Sichuan Provincial People's Hospital Chengdu 610072 Sichuan Province China

BACKGrOUND: It has been demonstrated that traditional antiepileptics, such as phenytoin, carbamazepine (CBZ), phenobarbital, etc., can result in the decrease of thyroid hormone of epileptic patients. However, there is still no sufficient evidence for the studies about the effect of new-type antiepileptics, such as topiramate (TpM), on thyroid hormones. OBJECTIVE: To observe the effects of TpM and CBZ on the level of thyroid hormones in serum of adults with epilepsy. DESIGN: A comparative observation. SETTING: Department of Neurology, sichuan provincial people's Hospital. pArTICIpANTS: Totally 100 outpatients or inpatients newly diagnosed to have epilepsy were selected from the Department of Neurology, sichuan provincial people's Hospital from July 2003 to August 2005, including 60 males and 40 females, aged 18-70 years. All the patients were accorded with the standard for the classification of epilepsy set by International League Against Epilepsy (ILAE) in 1981; Had been Informed and agreed with the detection; Had no history of thyroid gland disease; Had not taken any drugs could affect the thyroid function. Meanwhile, 40 adult healthy examinees were selected from our hospital as the control group, including 24 males and 16 females, aged 18-65 years. METHODS: ① The 100 epileptic patients were randomly divided into TpM group (n =50) and CBZ group (n =50), and they were treated with TpM (Xian-Janssen pharmaceutical, Ltd.; Batch number: 03AS032, Norm: 25 mg/tablet) and CBZ (Shanghai Sunve pharmaceutical Co., Ltd.; Batch number: 030201, Norm: 100 mg/tablet) respectively. The initial dosage of TpM was 25 mg per day, increased by 25 mg every week, the objective dosage of 100-200 mg per day was maintained when the symptoms were satisfactorily controlled. The dosage of CBZ was 6-8 mg/kg per day. All the patients were administrated for 1 year. ② The serum levels of total thyroxine (TT4), free thyroxine (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3) and thyr

关键词： CBZ Effects of topiramate and carbamazepine on thyroid hormone level in adults with epilepsy

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Quality control measures for lowering the seroconversion rate of hemodialysis patients with hepatitis B or C virus

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Hepatobiliary & pancreatic diseases International 2012年第3期11卷 302-306页

作者： Jing Yuan,Yi Yang, Fei Han, ping Zhang, Xiao-Ying Du, Hua Jiang and Jiang-Hua Chen The Kidney disease center, First Affiliated Hospital, Zhejiang University School of Medicine (Yuan J, Yang Y, Han F, Zhang p, Du XY, Jiang H and Chen JH) and Hemodialysis Quality control center of Zhejiang province (Yuan J, Zhang p, Du XY and Chen JH), Hangzhou 310003, china [a] The Kidney disease center First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China [b] Hemodialysis Quality control center of Zhejiang province Hangzhou 310003 China

BACKGrOUND: Hemodialysis (HD) patients are at high risk of infection by hepatitis B virus (HBV) or hepatitis C virus (HCV). The present study was designed to determine the impact of quality control measures on the prevention of transmission of blood-borne viruses. METHODS: A total of 6182 adult maintenance HD patients from all HD units in Zhejiang province were recruited on January 1, 2007. The baseline demographic and clinical characteristics were recorded and all patients were followed up until death or survival at 4 years later. The Quality control Standards of Hemodialysis were gradually implemented in HD units. The HBV or HCV seroconversion rates of the recruited patients were calculated and compared every year during the observation period. rESULTS: The prevalence of HBV was 8.3% at the beginning of the study, and 6.6% for HCV. With the implementation of the HD quality control measures, the HBV seroconversion rate tended to decrease year by year (χ 2 =6.620, p=0.085), and the HCV seroconversion rate decreased significantly (χ 2 =10.41, p=0.015). Compared with the data in 2007, the HBV seroconversion rate (χ 2 =4.204, p=0.040, relative risk ratio 0.393, 95% CI 0.156-0.991) and the HCV seroconversion rate (χ 2 =7.373, p=0.007, relative risk ratio 0.386, 95% CI 0.189-0.787) decreased significantly in 2010. CONCLUSION: Quality control measures for HD decreased the seroconversion rates of HBV or HCV in HD patients, showing that updated quality control measures reduce the risk for transmission of blood-borne viruses in the HD population.

关键词： hemodialysis quality control measures infection hepatitis B virus hepatitis C virus

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Construction of prophylactic Human papillomavirus Type 16 L1 Capsid protein Vaccine Delivered by Live Attenuated Shigella flexneri Strain sh42

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 743-750页

作者： Xiao-Feng YANG Xin-Zhong QU Kai WANG Jin ZHENG L(?)-Sheng SI Xiao-ping DONG Yi-Li WANG Key Laboratory of Biomedical Information Engineering of Ministry of Education,Institute for Cancer research,Xi'an Jiaotong University,Xi'an 710061.china National Institute for Viral disease control and prevention,Chinese center for disease control and prevention,Beijing 100052,china Department of Obstetrics and Gynecology,First Hospital of Xi'an Jiaotong University,Xi'an 710061,china KeyLab.ofBiomedicalInformationEngineeringofMinistryofEducation Inst.forCancerResearchXi'anJiaotongUnivers

To express human papillomavirus(HpV)L1 capsid protein in the recombinant strain of Shigellaand study the potential of a live attenuated Shigella-based HpV prophylactic vaccine in preventing HpVinfection,the icsA/virG fragment of Shigella-based prokaryotic expression plasmid pHS3199 was *** type 16 L1(HpV16L1)gene was inserted into plasmid pHS3199 to form the pHS3199-HpV16L1construct,and pHS3199-HpV16L1 was electroporated into a live attenuated Shigella strain *** analysis showed that HpV16L1 could be expressed stably in the recombinant strain *** test results were negative,which showed that the sh42-HpV16L1 lost ***,the at-tenuated recombinant strain partially maintained the invasive property as indicated by the HeLa cell *** IgG,IgA antibody against HpV16L1 virus-like particles(VLps)were detected in the sera,intestinal lavage and vaginal lavage from animals immunized by *** number of antibody-secreting cells in the spleen and draining lymph nodes were increased significantly compared with the *** from immunized animals inhibited murine hemagglutination induced by HpV16L1 VLps,whichindicated that the candidate vaccine could stimulate an efficient immune response in guinea pig's *** may be an effective strategy for the development of an HpV prophylactic oral vaccine.

关键词： human papillomavirus type 16 attenuated Shigella flexneri vaccine cervical cancer prophylactic

Lymphocyte Apoptosis in Vivo induced by Duck Swollenhead Hemorrhagic disease Virus

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Lymphocyte Apoptosis in Vivo induced by Duck Swollenhead Hem...

第四届第九次全国学术研讨会暨饲料和动物源食品安全战略论坛

作者： Li Chuanfeng Cheng Anchun Wang Mingshu Shen Chanjuan Zhang Na A Zhou Yi Chen Xiaoyue (Avian disease research center,College of Veterinary Medicine,sichuan Agricultural University,Yaan,sichuan 625014 china Key Laboratory of animal disease and Human Health of sichuan province,Yaan,sichuan 625014,china)

We studied apoptosis induced by duck swollenhead hemorrhagic disease virus (DSHDV) in vivo,focusing on the lymphoid organs that constitute the main targets for infection:bursa of Fabricius (BF), thymus,and spleen.72 ducks were inoculated intramuscularly with a virulent strain of *** morphology of lymphoid organs of these infected ducks was observed by light microscopy and transmission electron *** apoptosis was observed in DSHDV-infected BF,thymus, and *** apoptotic peak appeared at 72h *** death by classic necrosis was also observed in dead ducks after 72h postinoculation.,and it was further confirmed by in situ terminal deoxynucleotidyl transferase dUTp nick-end labeling (TUNEL).We conclude in DSHDV-infected ducks that apoptosis of lymphocytes resulted in the depletion of iymphocytes,may play an important role in the pathogenesis of duck viral swollenhead hemorrhagic disease.

关键词： duck swollenhead hemorrhagic disease virus apoptosis necrosis lymphocyte

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中国艾滋病诊疗指南(2024版)

中华传染病杂志 2024年第5期42卷 257-284页

作者：中华医学会感染病学分会艾滋病学组中国疾病预防控制中心李太生沈银忠不详中国医学科学院北京协和医学院北京协和医院感染内科北京100730 上海市(复旦大学附属)公共卫生临床中心感染与免疫科

中华医学会感染病学分会艾滋病学组于2005年制订了我国《艾滋病诊疗指南》(以下简称《指南》),2024版《指南》是在2021年第5版《指南》的基础上参照国内外最新研究进展修订而成。新版《指南》重点对抗病毒治疗、全程管理、机会性感染、... 详细信息

中华医学会感染病学分会艾滋病学组于2005年制订了我国《艾滋病诊疗指南》(以下简称《指南》),2024版《指南》是在2021年第5版《指南》的基础上参照国内外最新研究进展修订而成。新版《指南》重点对抗病毒治疗、全程管理、机会性感染、人类免疫缺陷病毒(HIV)合并肿瘤、HIV感染的预防与干预等内容进行了更新,并新增"艾滋病免疫功能重建不全"这部分内容,首次提出了"艾滋病脆弱人群"的概念,增加了诊治推荐意见及其推荐证据和推荐强度。新版《指南》包括流行病学、病原学特征、实验室检测、发病机制、临床表现与分期、诊断标准、常见机会性感染、抗病毒治疗、免疫重建炎症综合征、免疫功能重建不全、艾滋病相关肿瘤、HIV母婴传播阻断及单阳家庭生育、HIV暴露前后预防与阻断、HIV感染的全程管理等14个方面的内容。本《指南》旨在帮助临床医师在艾滋病诊治和管理中合理决策,《指南》也将根据国内外的研究进展定期进行修订与更新。

关键词：获得性免疫缺陷综合征人类免疫缺陷病毒诊断治疗指南

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中国艾滋病诊疗指南(2024版)

中国艾滋病性病 2024年第8期30卷 779-806页

作者：中华医学会感染病学分会艾滋病学组中国疾病预防控制中心李太生沈银忠不详中国医学科学院北京协和医学院北京协和医院感染内科上海市(复旦大学附属)公共卫生临床中心感染与免疫科

中华医学会感染病学分会艾滋病学组于2005年制订了我国《艾滋病诊疗指南》(简称《指南》),2024版《指南》是在2021年第5版《指南》的基础上参照国内外最新研究进展修订而成。新版《指南》重点对抗病毒治疗、全程管理、机会性感染、HIV... 详细信息

中华医学会感染病学分会艾滋病学组于2005年制订了我国《艾滋病诊疗指南》(简称《指南》),2024版《指南》是在2021年第5版《指南》的基础上参照国内外最新研究进展修订而成。新版《指南》重点对抗病毒治疗、全程管理、机会性感染、HIV合并肿瘤、HIV感染的预防与干预等内容进行了更新,并新增“艾滋病免疫重建不全(INr)”这部分内容,首次提出了“艾滋病脆弱人群”的概念,增加了诊治推荐意见及其推荐证据和推荐强度。《指南》包括流行病学、病原学特征、实验室检测、发病机制、临床表现与分期、诊断标准、常见机会性感染、抗病毒治疗、免疫重建炎性综合征、INr、艾滋病相关肿瘤、HIV母婴垂直传播阻断及单阳家庭生育、HIV暴露前后预防与阻断、HIV感染的全程管理等14个方面的内容。本《指南》旨在帮助临床医师在艾滋病诊治和管理中合理决策,《指南》也将根据国内外的研究进展定期进行修订与更新。

关键词：获得性免疫缺陷综合征人类免疫缺陷病毒诊断治疗指南

The quantity influence of Bifidobacterium, Bacillus,and enterococcus in digestive tract of Duck Immunized with Low Duck plague Virus by Oral Innoculation

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The quantity influence of Bifidobacterium, Bacillus,and ente...