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Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 257-261页

作者： Wei-Jun Jiang, Xiao-Bo Man, Liang Tang, Hong-Yin Song, Shen-Jing Li, Guo-Jun Cai, Xiu-Hua Qiu and He-Ping Hu department of Gastroenterology, shanghai Tongren hospital, shanghai 200050, china department of Gastroenterology, Eastern Hepatobiliary surgery hospital, second military medical university, shanghai 200438, china department of Phannacology, second military medical university, shanghai 200433, china department of Gastroenterology Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China.

BACKGROUND: OCI-5, the rat homologene of human glypican 3 ( GPC3), is confirmed upregulated in hepatocellular carcinoma (HCC). The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC. METHODS: Male Sprague-Dawley rats were given diethyl-nitrosamine ( DENA) to induce HCC. Three DENA-induced rats and one control rat were sacrificed every week for 18 weeks during the development of HCC. Tissues specimens were snap-frozen in liquid nitrogen and total RNA was isolated. Sk-Hepl cells were treated with DENA at different concentrations. The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method. RESULTS: OCI-5 was not expressed in normal rat liver tissues. When HCC occurred and aggravated, OCI-5 expression was gradually elevated to a very high level. GPC3 was not expressed in the DENA-treated Sk-Hepl cells. CONCLUSIONS: OCI-5 was not expressed in normal rat liver tissues but in rat HCC tissues. High-expression of OCI-5 in DENA-induced rat HCC model was the gene expression change of HCC not the DENA-induced gene expression. The expression level of OCI-5 was not only elevated in rat HCC but also gradually along the occurrence and progression of HCC, indicating that GPC3 might serve as a sensitive marker of early stage HCC.

关键词： OCI-5 gene expression carcinoma, hepatocellular rat

Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats

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Acta Pharmacologica Sinica 2004年第5期25卷 69-74页

作者： Yan-ting WANG2, Shu HAN2, Kai-hua ZHANG2, Yu JIN3, Xiao-ming XU2,4, Pei-hua LU2,52department of Neurobiology, shanghai second medical university, shanghai 200025, china 3department of surgery,Xinhua hospital Affiliated to shanghai second medical university 4Kentucky Spinal Cord Injury Research Center,department of Neurological surgery, university of Louisville School of Medicine, Louisville, KY, 40292, USA department of Neurobiology Shanghai Second Medical University 上海 200025

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM ex-pression and cellular localization were examined using Northern blot, RT-PCR, immunohistochemistry and immun-ofluorescence double-labeling methods. RESULTS: HB-GAM mRNA was significantly upregulated in spinal cordtissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d,peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was co-localized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinaltransection. CONCLUSION: SCI induces HB-GAM expression at both mRNA and protein levels in areas close tothe injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process ofinjury and/or repair following SCI.

关键词： heparin-binding growth-associated molecule spinal cord injuries immunohistochemistry reversetranscriptase polymerase chain reaction astrocytes neurons

Crosstalk between tumor cells and microenvironment via Wnt pathway in colorectal cancer dissemination

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World Journal of Gastroenterology 2008年第12期14卷 1823-1827页

作者： Dan Huang, Xiang Du, department of Pathology, Cancer hospital of Fudan university department of Oncology, shanghai medical College, Fudan university, shanghai 200032, china department of Pathology Cancer Hospital Fudan University Shanghai 200032 China.

Invasion and metastasis are the deadly face of malignant tumors. Considering the high rate of incidence and mortality of colorectal cancer, it is critical to determine the mechanisms of its dissemination. In the parallel investigation of the invasive front and tumor center area of colorectal cancer (CRC), observation of heterogeneous β-catenin distribution and epithelial-mesenchymal transition (EMT) at the invasive front suggested that there might be a crosstalk between tumor cells and the tumor microenvironment. Wnt signaling pathway is also involved in the cancer progression due to its key role in CRC tumorigenesis. Moreover, in recent years, there is increasing evidence that the regulators of microenvironment, including extracellular matrix, growth factors and inflammatory factors, are associated with the activation of Wnt pathway and the mobility of tumor cells. In this review, we will try to explain how these molecules trigger metastasis via the Wnt pathway.

关键词： Invasion Microenvironment Colorectalcancer Epithelial-mesenchymal transition Wnt β-catenin

Inhibition of hepatitis B virus by oxymatrine in vivo

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World Journal of Gastroenterology 2001年第1期7卷 49-52页

作者： Xiao Song Chen1 Guo Jun Wang1 Xiong Cai1 Hong Yu Yu2 Yi Ping Hu3 1department of Infectious Diseases, changzheng hospital, the second military medical university, shanghai 200003, china2department of Pathology, 3department of Cell Biology, department of Basic Medicine, the second military medical university, shanghai 200433, china department of Cell Biology the Second Military Medical University Shanghai 200433China. Yphu@***

AIM To investigate the anti-HBV effect ofoxymatrine (oxy) in *** HBV transgenic mice were producedby micro-injection of a 4.2kb fragmentcontaining the complete HBV *** level of HBsAg and HBcAg in thetransgenic mice liver was determined byimmunohistochemical *** Four groups (6 mice in each group)were injected intraperitoneally with oxy at thedosage of 100,200, and 300 mg/kg or with salineonce a day for 30 days. Both HBsAg and HBcAgwere positive in livers of all the six mice in thecontrol group (injected with saline), and werepositive in livers of two mice in 100 mg/kg groupand 300mg/kg group. In 200mg/kg group,HBsAg and HBcAg were negative in livers of allthe six mice. Based on the results, 200 mg/kg isthe ideal dosage to explore the effect of oxy atdifferent time points. According to the oxytreatment time, mice were divided into fourgroups: 10 d, 20 d, 30 d and 60 d (4 mice in eachgroup). Each mouse underwent liver biopsy twoweeks before the treatment of oxy. Down-regulation of HBsAg and HBcAg appeared aftertreatment of oxymatrine for 10 d and 20 d, Dane-like particles disappeared after the treatment ofoxy for 20d under electron microscopy,however, the expression level of HBsAg andHBcAg returned to normal 60 d later after *** oxymatrine can reduce thecontents of HBsAg and HBcAg in transgenic miceliver, longer treatment time and larger dosagedo not yield better effects.

关键词： Alkaloids Animals Antiviral Agents DNA, Viral Dose-Response Relationship, Drug Gene Expression Regulation, Viral Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis B virus development Mice Mice, Transgenic Research Support, Non-U.S. Gov't Virus Replication

Blood pressure,baroreflex sensitivity,and end organ damage in hybrid offspring of spontaneously hypertensive rats and Spraque-Dawlev rats

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Acta Pharmacologica Sinica 2005年第9期26卷 1049-1056页

作者： He-hui XIE~2 Fu-ming SHEN~2 Chao-yu MIAO Ding-feng SU~3 department of Pharmacology,second military medical university,shanghai 200433,china departmentofPharmacology SecondMilitaryMedicalUniversityShanghai200433China

Aim:To investigate the blood pressure(BP),baroreflex sensitivity(BRS),andorgan damage in hybrids of spontaneously hypertensive rats and ***:Spontaneously hypertensive rats and Sprague-Dawley rats werecrossbred,and the F1hybrids were inbred randomly to produce an *** the age of 52 weeks,the F1and F2hybrids were tested to determine BP and BRSin a conscious *** examinations were carried out after BPrecording and BRS ***:BP and BRS were not different in F1and *** was inversely related to systolic BP(SBP)in male,female,or wholepopulations of ***,BRS values were one-third determined bySBP level(the determinant coefficient was 0.326).The indexes for left ventricularhypertrophy,aortic hypertrophy,and renal damage were all positively related toBP,and negatively related to *** multiple-regression analysis,left ventricularand aortic hypertrophy and glomerulosclerosis score were all most significantlyassociated with lower BRS and higher systolic *** contribution of BRS to leftventricular and aortic hypertrophy and glomerulosclerosis was greater than thatof ***:The present work with hybrid rats demonstrated quantita-tively that the BRS value was one-third determined by SBP *** BP leveland BRS value contributed greatly to the hypertensive organ ***,the contribution of BRS to the hypertensive organ damage was greater than thatof BP level in these rats.

关键词： baroreflex blood pressure end organ damage hypertension genetic hybridization

Effects of Changtai granules, a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats

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World Journal of Gastroenterology 2005年第23期11卷 3539-3543页

作者： Yong-Bing Cao,Jun-Dong Zhang,Lan Yan,De-Jun Wang,Xin-Ming Jia,Ping-Hui Gao,Ming-He Cheng,Zheng Xu,Yan Wang,Yuan-Ying Jiang,department of Pharmacology,School of Pharmacy,second military medical university,shanghai 200433,china Ya-Ying Diao,department of Pharmacy,Changhai hospital,second military medical university,shanghai 200433,china department of Pharmacology School of Pharmacy Second Military Medical University Guohe Road 325 Shanghai 200433 China.

AIM: To study the effects of Changtai granules (CTG), a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats. METHODS: Healthy adult Sprague-Dawley (SD) rats of both sexes, weighing 250-300 g, were employed in the present study. The rat colitis models were induced by 2, 4,6-trinitrobenzene sulfonic acid (TNBS) enemas at a concentration of 100 mg/kg in 50% ethanol. The experimental animals were randomly divided into dexamethasone (DX) treatment, CTG treatment, and model control groups, which were intracolicly treated daily with DX (0.2 mg/kg), CTG at doses of 2.9, 5.7 and 11.4 g crude drug/kg, and the equal amount of saline respectively from 6 h following induction of the colitis in rats inflicted with TNBS to the end of study. A normal control group of rats treated without TNBS but saline enema was also included in the study. After 3 wk of treatment, the animals were assessed for colonal inflammatory and ulcerative responses with respect to mortality, frequency of diarrhea, histology and myeloperoxidase activity (MPO).RESULTS: The therapeutic effect of CTG on ulcerative colitis (UC) was better than DX. CTG effectively inhibited the activity of granulocytes, macrophages and monocytes in a dosedependent manner. Also it reduced MPO and formation of inflammation in colonic mucosal tissue. Furthermore, administration of CTG significantly prevented body mass loss and death, and decreased frequency of diarrhea in UC rats, when compared with the model control group ***: CTG would prove to be an ideal drug for chronic UC, and is warranted to be studied further.

关键词： Ulcerative colitis Changtai granules 2,4,6-Trinitrobenzene sulfonic acid Myeloperoxidase

Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells

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World Journal of Gastroenterology 2001年第1期7卷 98-101页

作者： Hao Ren Fen Lu Zhu Shi Ying Zhu Yan Bin Song Zhong Tian Qi department of Microbiology, second military medical university, shanghai 200433, china department of Microbiology Second Military Medical University Shanghai 200433 China. qizt@***

INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agen... 详细信息

关键词： Animals Antibodies, Viral Blotting, Western Cell Line Electrophoresis, Polyacrylamide Gel Flaviviridae Infections GB virus C purification Gene Expression Regulation, Viral Plasmids Polymerase Chain Reaction Recombinant Proteins Research Support, Non-U.S. Gov't Spodoptera Transfection Viral Nonstructural Proteins

Killing effects of cytosine deaminase gene mediated by adenovirus vector on human pancreatic cancer cell lines in vitro

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 147-151页

作者： Zhao-Shen Li Xue Pan Guo-Ming Xu Long Cui Guan-Rong Dai Yan-Fang Gong Zhen-Xing Tu the department of Gastroenterology department of general surgery Changhai hospital, second military medical university, shanghai 200433, china department of Gastroenterology Changhai Hospital Second Military Medical University Shanghai 200433 China. zsli@***

OBJECTIVE: To evaluate the killing effects of the cytosine deaminase (CD) gene mediated by adenovirus vector on human pancreatic cancer cell lines in vitro. METHODS: The CD gene was cloned into pAdTrack-CMV-CD, and pAdTrack-CMV-CD and pAdEasy-l were recombinated in bacteria. The newly recombinated Ad-CD containing green fluoreseent protein (GFP) was propagated in 293 cells and purified by cesium chloride gradient centrifugation. Human pancreatic cancer cell lines Patu8988 and SW1990 were infected with this virus, then 5-FC was added. XTT assay was used to estimate relative numbers of viable cells. RESULTS: The positive clones were selected by using endonuclease to digest the combinatants and the concentration of viral liquids containing the CD gene was 2×1O^(11) pfu/ml. It was found that significant cytotoxic activities were possesscd by 5-FC for the CD gene transduced pancreatic cell lines, but little effects exerted on the nontransduced pancreatic carcinoma cells. CONCLUSIONS: The CD gene mediated by adenovirus with a high infectivity is efficient for gene therapy of pancreatic carcinoma cell lines. These data demonstrate the therapeutic efficacy of an enzyme prodrug strategy in experimental pancreatic cancer.

关键词： pancreatic cancer adenovirus verctor cytosine deaminase gene therapy

Regulation of Toll-like receptor signaling in innate immunity

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Science china(Life Sciences) 2010年第1期53卷 34-43页

作者： AN HuaZhang,QIAN Cheng & CAO XueTao National Key Laboratory of medical Immunology & Institute of Immunology,second military medical university,shanghai 200433,china National Key Laboratory of medical Immunology & Institute of Immunology Second Military Medical University Shanghai China

Toll-like receptors sense invading pathogens by recognizing a wide variety of conserved pathogen-associated molecular patterns(PAMPs).The members of the TLR family selectively utilize adaptor proteins MyD88,TRIF,TIRAP and TRAM to activate overlapping but distinct signal transduction pathways which trigger production of different panels of mediators such as proinflammatory cytokines and type I *** mediators not only control innate immunity but also direct subsequently developed adaptive *** activation is strictly and finely regulated at multiple levels of the signal transduction pathways.

关键词： Toll-like receptors TLR signaling