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Protein phosphorylation is involved in the water stress-induced ABA accumulation in the roots of Malus hupehensis Rehd

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Chinese Science Bulletin 2001年第10期46卷 855-858页

作者： ZHANG Dapeng, YANG Hongqiang, JIA Wensuo & HUANG Conglin1. Laboratory of Molecular Developmental Biology of Fruit Trees, china agricultural university, Beijing 100094,{2. 2. The Key Laboratory of Plant Physiology and Biochemistry of agricultural Ministry, china agricultural university, Beijing 100094,{2. Laboratory of Molecular Developmental Biology of Fruit Trees China Agricultural University Beijing China The Key Laboratory of Plant Physiology and Biochemistry of agricultural Ministry China Agricultural University Beijing China

Water stress-induced ABA accumulation plays a key role in the root to shoot communication and/or the cell to cell signaling under the soil stresses. The signaling of the water stress itself that leads to the accumulation, however, is less known. In this study, we subjected the roots of Malus hupehensis seedlings to water stress treatment and investigated the ABA accumulation in relation to protein phos-phorylation. Our results showed that ABA accumulation could be substantially triggered in 40 min and reached 4 folds in 100 min after treatment with 30% PEG 6.00 (polyethylene glycol). The water stress treatment also led to a substantial enhancement of total kinase activity, assessed with histone-111 as substrate, in 15 min and a maximum enhancement in 30 min before it declined to initial level. The Ca2.-dependent kinase activity showed a similar, if not more sensitive, trend. When the roots were fed with labeled 32.-ATP, water stress enhanced the labeling of proteins, which showed a maximum labeling at

关键词： ABA (abscisic acid) water stress protein kinases Malus hupehensis Rehd.

Seasonal influence on the development of parthenotes and cloned embryos in pigs

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Progress in Natural Science:Materials International 2007年第10期17卷 1172-1176页

作者： Zhang Kun1, Zhang Yunhai3,4, Pan Dengke5, Ma Yufang1, Wei Hengxi1, Zhang Jian6. Li Xuyang7, Wang Shaohua1, Li Yan2. Dai Yunping1,2.and Li Ning1(1. State Key Laboratory for Agrobiotechnology, College of Biological Science, china agricultural university, Beijing {2., china 2. JiPulin Biotech Ltd., Beijing {2., china 3. Danish Institute of agricultural Sciences, 8830 Tjele, Denmark 4. College of Animal Science and Technology, Anhui agricultural university, Hefei 2.0036. china 5. Institute of Animal Sciences, Chinese Academy of agricultural Sciences, Beijing {2., china 6. Animal Clinical Hospital, china agricultural university, Beijing {2., china 7. Hebei Minghui Group of Pigs Breeding, Sanhe 06.2.0, china) State Key Laboratory for Agrobiotechnology College of Biological Science China Agricultural University Beijing 100094 China JiPulin Biotech Ltd. Beijing 100094 China Danish Institute of agricultural Sciences 8830 Tjele Denmark College of Animal Science and Technology Anhui Agricultural University Hefei 230036 China Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China 7. Hebei Minghui Group of Pigs Breeding Sanhe 065200 China

Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (P<0.05). However, no significant difference was found in the rate of oocytes progressing to MⅡ in four seasons (P<0.05). In addition, the rate of blastocyst formation of parthenogenetic embryos in summer was obviously declined compared to the other seasons (P<0.05), and an increased blastocyst rate of cloned porcine embryos was found in spring compared to autumn and winter (P<0.05). The results suggest that there should be a seasonal influence on the developmental competence of porcine embryos.

关键词： pig cloned parthenogenetic season

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural Science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2. Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. College of Animal Science and Technology, china agricultural university, Beijing 100094,{2. 2. College of Biological Science, china agricultural university, Beijing 100094,{2. 3. College of Animal Science and Technology, Yunnan {6. university, Kunming 6.02.1,{2.) College of Animal Science and Technology China Agricultural University Beijing 100094 China College of Biological Science China Agricultural University Beijing 100094 China College of Animal Science and Technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2.2.(E4)-Asn and Ser-2.79(E4)-Gly were identified in exon 4 of the gene. The Lys-2.2.(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2.2.(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nif promoter analysis

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Science china(Life Sciences) 2006年第2期49卷 115-122页

作者： ZHAO Hongxin1,2.3, XIE Baoen2.& CHEN Sanfeng1,2.1. State Key Laboratory for Agrobiotechnology and Biological School, china agricultural university, Beijing 100094,{2. 2. Key Laboratory for Agro-Microbial Resource and Application of Agriculture Ministry, china agricultural university, Beijing 100094,{2. 3. Chemical and Biological School, ShenYang Normal {6., Shenyang 110034,{2. 1. State Key Laboratory for Agrobiotechnology and Biological School China Agricultural University Beijing 100094 China 2. Key Laboratory for Agro-Microbial Resource and Application of Agriculture Ministry China Agricultural University Beijing 100094 China 3. Chemical and Biological School ShenYang Normal University Shenyang 110034 China

A 32. bp of nifH fragment was PCR amplified from Paenibacillus massiliensis T7 using the universal degenerate primers. The PCR-amplified nifH fragment was labeled with DIG; then used as a probe in Southern blot analysis. Southern blot result showed that there were two positive signals, indicating that there might be two copies of nifH in P. massiliensis T7. A total of 102.4 bp DNA sequence containing purD; nifBHDKENX was obtained by five rounds of inverse-PCR amplification. The predicted proteins of nifBHDKENX had high homology with those from other nitrogen-fixing bacteria. Only one putative σ[54]-dependent promoter sequence was detected upstream of the nifB gene; nifBHDKENX were likely to be organized in one operon. Assays of β-galactosidase activity of P. massiliensis T7PB carrying a nifB-lacZ fusion under different concentrations of NH4 [+]; O2.showed that the expression of nifB-lacZ was strongly inhibited by O2.

关键词： Paenibacillus massiliensis T7, nifBHDKENX, nifB-lacZ, inverse-PCR.

Effects of elevated CO_2.on growth and carbon partitioning in rice

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Chinese Science Bulletin 1998年第23期43卷 1982-1986页

作者： LIN Weihong\+1 and WANG Dali\+2.1. Department of Plant Nutrition, china agricultural university, Beijing 100094,{2. 2. Department of Systems Ecology, Research center for Eco_Environmental Science, Chinese Academy of Sciences, Beijing 100085,{2. Department of Plant Nutrition China Agricultural University Beijing China Department of Systems Ecology Research Center for Eco-Environmental Science Chinese Academy of Sciences Beijing China

Rice (\%Oryza sativa\% cv. Jindao 1187) was grown in open_top chambers which contained ambient and enriched CO\-2. CO\-2.elevation stimulated rice tillering during early vegetative stage. However, panicle dry weight per plant did not change at maturity stage. Root biomass was enhanced by high CO\-2. Root / shoot ratio was increased under high CO\-2.at maturity, indicating more carbon allocation to the below_bround part in rice under high CO\-2.

关键词： CO 2 elevation growth carbon partitioning rice (\%Oryza sativa cv\%. Jindao 1187).

Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer

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Science china(Life Sciences) 2006年第2期49卷 164-171页

作者： ZHANG Yunhai1, PAN Dengke1,2. SUN Xiuzhu1, SUN Guojie1, WANG Xiaobo1, LIU Xiaohui1, LI Yan1, DAI Yunping1 & LI Ning1 1. State Key Laboratory for Agrobiotechnology, china agricultural university, Beijing 100094,{2. 2. College of Animal Science and Technology, china agricultural university, Beijing 100094,{2. State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China College of Animal Science Technology China Agricultural University Beijing 100094 China

In the present study, nuclear transferred embryos (NTEs) were reconstructed by using pig fetal fibroblasts as donors; in vitro matured oocytes as recipients. The effects of G418 selection on donor cells, duration of IVM of prepubertal gilt oocytes; oxygen tension in IVM of oocytes were investigated. The results were as follows: (i) When G418 selected cells expressing GFP were used as donors, the cleavage rate of NTEs decreased drastically in comparison to NTEs derived from donors without antibiotic selection (47.5% vs. 71.6., p>0.05). For the blastocyst rate, no significant difference was observed between two groups (10% vs. 10.4%, p6.to 42.h (83.6. vs. 96.7%, p>0.05). However, no statistical difference was observed between NTEs derived from oocytes of 36.h IVM group; NTEs from oocytes of 42.h IVM group in the rates of cleavage (59.3% vs. 73.6., p2., p2. tension; NTEs derived from oocytes matured under higher oxygen tension (2.% O2. in cleavage rate (70.6. vs. 6..1%, p2.3%, p6.to 42.h, while no significant differences were observed for developmental potential of transgenic embryos. Thus IVM 42.h is the better choice in order to obtain maximum number of MII oocytes as recipients. (iii) Lower oxygen tension; higher oxygen tension in IVM have no significant effect on development of cloned embryos.

关键词： transgenic cloning, GFP, Pig, early development.

Tissue-specific expression of glutathione S-transferases induced by 2.tridecanone or quercetin in cotton bollworms, Helicoverpa armigera (Hübner)

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Progress in Natural Science:Materials International 2005年第11期15卷 988-992页

作者： TANG Fang, LIANG Pei and GAO Xiwu (Department of Entomology, china agricultural university, Beijing 100094,{2.) Department of Entomology China Agricultural University Beijing 100094 China

The tissue-specific expression of glutathione S-transferases (GSTs) in the cotton bollworm and the expression level induced by 2.tridecanone and quercetin were examined using the methods of biochemistry and the quantitative PCR. The relative expression level of GST mRNA was unanimous with the GSTs activity conjugaging with 1-chloro-2. 4-dimitro-benzene (CDNB) in fat bodies, midguts, heads and integuments of cotton bollworms. The GSTs activity in fat bodies was the highest, then midguts, heads and integuments in turn, which was in consistent with the relative expression level of GST mRNA. The specific activity of GSTs and the relative expression level of GST mRNA could be significantly induced by 2.tridecanone and quercetin, and after the induction the order of the GSTs activity and the relative expression level of GST mRNA in the above four tissues in cotton bollworms was not different from the control. The induction of GSTs by 2.tridecanone was stronger than by quercetin in all four tissues, which was in accordance with the relative expression level of GST mRNA. It suggested that the increase of GSTs activity induced by plant allelochemicals was associated with the elevated expression of GST mRNA in cotton bollworms.

关键词： Helicoverpa armigera glutathione S-transferase tissue-specific expression plant allelochemicals.

Research on the method for retrieving soil moisture using thermal inertia model

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Science china Earth Sciences 2006年第5期49卷 539-545页

作者： LIU Zhenhua1 & ZHAO Yingshi2.1. Information College, South china agricultural university, Guangzhou 5106.2.{2. 2. Graduate university of Chinese Academy of Sciences, Beijing 100049,{2. 1. Information College South China Agricultural University Guangzhou 510642 China 2. Graduate university of Chinese Academy of Sciences Beijing 100049 China

In order to improve accuracy of soil moisture inversion using remote sensing, a new thermal inertia model is proposed in this paper. The improved model needs only surface maximum temperature as the temperature parameter input instead of input of the surface temperature difference, as well as the surface sensible and latent fluxes are introduced into boundary conditions of thermal conductivity equation. Furthermore, surface soil conductive heat transfer equation of two-layer model is used to solve the soil thermal inertia so that the remote sensing thermal inertia method can be ap- plied to regions with better-covered vegetation, but usually only for the bare areas or worse vegetation covered areas. The model has been tested at several locations in the area of west Inner Mongolia. Comparing the simulation of the new model with the measurements obtained by apparent thermal inertia and by field test, the result shows that the inertia thermal model can be used to estimate soil moisture in more reasonable accuracy.

关键词： thermal model, thermal conductivity equation, soil initial, sensible flux, latent flux, two-layer model.

Purification of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 297-302页

作者： Yan-Qin LIU1,2. Zhen-Jun SUN1*, Chong WANG1, Shi-Jie LI2. and Yu-Zhi LIU2.College of Resource and Environment Science, china agricultural university, Beijing 100094,{2. 2.ollege of Animal Science, Hebei agricultural university, Baoding 071001,{2. College of Resource and Environment Science China Agricultural UniversityBeijing 100094 China College of Animal Science Hebei Agricultural UniversityBaoding 071001 China

A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52.ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOF MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP312., was obtained, with the molecular mass of 510.8 Da and the sequence being “ACSAG”.

关键词： antibacterial peptide earthworm purification