检索结果-南通市图书馆

Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3) Jian FEI~2 Da-Fu CUI~1 You-Shang ZHANG~1 ~1institute of biochemistry and cell biology,and ~2Model Organism Research Center,shanghai institutes for Biologicol sciences, chinese academy of sciences,shanghai 200031,china ~3Graduate School of the chinese academy of sciences,shanghai 200031,china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Graduate School of the chinese academy of sciences shanghai 200031 China Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Monomeric B27 Lys destripeptide insulin (B27 Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B27 Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B27 Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B27 Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 200 mg per liter of culture,and the overall yield ofpurified B27 Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B27 Lys DTrI asdetermined by the mouse convulsion assay was 21 U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B27 Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome(Schistosomajaponicum)by RNA Interference

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第6期37卷 386-390页

作者： Guo-Feng CHENG~1 Jiao-Jiao LIN~1 Yi SHI~2 You-Xin JIN~2 Zhi-Qiang FU~1 Ya-Mei JIN~1 Yuan-Cong ZHOU~2 You-Min CA~1 1 shanghai institute of Animal Parasitology, chinese academy of Agricultural sciences, Key Lahoratory of Animal Parasitology,Ministry of Agriculture, shanghai 200232, china 2 State Key Laboratory, of Molecular biology institute of biochemistry and cell biology shanghai institutes for biological sciences, chinese academy of sciences. shanghai 200031, china shanghai institute of Animal Parasitology Chinese Academy of Agricultural Sciences Key Laboratory of Animal Parasitology Ministry of Agriculture Shanghai 200232 China.

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary forthe pairing between the male and female worms is specifically expressed in the adult male *** is widely distributed in the adult female worm after *** transcription-polymerase chainreaction(RT-PCR)and immunofluorescence were employed to analyze the relationship between the RNAieffect and dsRNA dosage in the *** results revealed that the inhibition of SjGCP expression bysiRNA is ***-PCR analysis showed that the SjGCP transcript level was reduced by 75%when 100 nM dsRNA was applied.

关键词： RNA interference gynecophoral canal protein gene Schistosoma japonicum dosage

Pretreatment with insulin enhances anticancer functions of 5-fluorou racil in human esophageal and colonic cancer cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第5期28卷 721-730页

作者： Ke ZOU~(1,2),Ji-hang JU~(1,2),Hong XIE~(1,3)~1 institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china ~2 Graduate School of the chinese academy of sciences,Beijing,100049,china instituteofbiochemistryandcellbiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China GraduateSchoolofthechineseacademyofsciences Beijing100049China

Aim:To investigate the effects of insulin on enhancing 5-fluorouracil (5-FU) anti-cancer functions and its mechanisms in the human esophageal cancer cell line(Eca 109) and human colonic cancer cell line (Ls-174-t).Methods:The effect ofinsulin/5-FU combination treatment on the growth of Eca 109 and Ls-174-t cellswas evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT) *** insulin treatment or insulin/5-FU treatment,cell cycle distri-bution of both cell lines was analyzed by flow *** blot assay wasused to assess the expression of caspase-3 and thymidylate synthase (TS).Apoptosis was detected by flow cytometry,DNA fragmentation assay,and termi-nal transferase dUTP nick end labeling assay (TUNEL).Moreover,the changes of5-FU uptake after insulin pretreatment were detected by HPLC assay and Westernblot ***:We found that insulin enhanced the inhibitory effect of 5-FU on cell proliferation when Eca 109 cells and Ls-174-t cells were pretreated withinsulin for the appropriate *** increased the cell number of the S phaseand the uptake of ***/5-FU treatment enhanced apoptosis of tumor cellsand upregulated the expression of cleaved caspase-3 compared with 5-FU ***,insulin/5-FU treatment induced the changes of free TS and the TSternary complex level compared with 5-FU treatment in Eca 109 and Ls-174-t ***:These data suggest that insulin enhances anticancer functions of 5-FU when it is treated before 5-FU for the appropriate time in human esophagealand colonic cancer cell lines.

关键词： apoptosis cell cycle 5-fluorouracil insulin

Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 1996年第1期6卷 1-9页

作者： ZAN HONG MING YEH (shanghai institute of cell biology, chinese academy of sciences, shanghai 200031, china.) shanghai institute of cell biology Chinese Academy of Sciences Shanghai China

Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.

关键词： Phage antibody library human IgE(Fab) human anti-trichosanthin IgE

Identification of γ-aminobutyric acid transporter (GAT1)on the rat sperm

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2000年第1期10卷 51-58页

作者： HU JING HUA XIAO BING HE YUAN CHANG YAN (shanghai institute of cell biology, chinese academy of sciences, shanghai 200031) shanghaiinstituteofcellbiology ChineseAcademyofSciences.

Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence of 67kDa GAT1 protein and mRNA in rat testis by Western blotting and reverse transcription-polymerase chain reaction. Meanwhile, immunohistochemical and immunofluorescent analyses also identified GAT1 protein on the elongated spermatid and sperm. These results indicated that rat testis is a novel site of gat1 expression. Further studies should be taken to explore the role of GAT1 protein on sperm acrosome reaction.

关键词： Gat1 rat testis sperm expression.

Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第1期38卷 22-28页

作者： Ming-Min ZHAO~(1,2),De-Rong AN~(1*),Jian ZHAO~2,Guang-Hua HUANG~3,Zu-Hua HE~4,and Jiang-Ye CHEN~3 ~1 College of Plant Protection,Northwest Science and Technology University of Agriculture and Forestry,Yangling 712100,china ~2 College of Agriculture,Yangtze University,Jingzhou 434025,china ~3 State Key Laboratory of Molecular biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china ~4 State Key Laboratory of Plant Molecular Genetics,institute of Plant Physiology and Ecology,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200032,china College of Plant Protection Northwest Science and Technology University of Agriculture and Forestry Yang ling 712100 China College of Agriculture Yangtze University Jingzhou 434025 China State Key Laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in asequence-specific *** interfering RNA (siRNA),an intermediate of the RNAi pathway,has beenshown to be very effective in inhibiting virus infection in mammalian cells and cultured plant ***,wereport that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) couldinhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso-ciated 126 kDa protein in intact plant *** results indicate that transiently expressed shRNA efficientlyinterfered with TMV *** interference observed is sequence-specific,and time-and *** expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumbermosaic virus (CMV),an unrelated *** order to interfere with TMV accumulation in tobaccoleaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV *** support the view that RNAi opens the door for novel therapeutic procedures against virus *** propose that a combination of the RNAi technique and Agrobacterium-mediated transient expressioncould be employed as a potent antiviral treatment in plants.

关键词： tobacco mosaic virus 126 kDa protein RNA interference short hairpin RNA

Toosendanin interferes with pore formation of botulinum toxin type A in PC12 cell membrane

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2006年第1期27卷 66-70页

作者： Mu-feng LI Yu-liang SHI institute of Physiology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china instituteofPhysiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China

Aim:Botulinum neurotoxins (BoNT) abort the process of neurotransmitter re-lease at presynaptic motor nerve terminals,causing muscle *** abilityof botulinum toxin to produce its effect is dependent on the ability of the lightchain to cleave the SNARE proteins associated with transmitter ***-cation of the light chain protease through the heavy chain-formed channel is apivotal step in the intoxication *** (TSN),a triterpenoid de-rivative extracted from a chinese traditional medicine,has been demonstrated tobe an effective cure for experimental *** study was designed to explorethe antibotulismic mechanisms of ***:The inside-out single-channel recording patch-clamp technique was used to record the BoNT/A-in-duced currents in the presence and absence of ***:Channel formationwas delayed and the sizes of the channels were reduced in the TSN-treated PC12cell ***:The antibotulismic effect of TSN might occur viainterference with toxin translocation.

关键词： toosendanin botulinum toxin type A ion channel antibotulismic mechanism single channel recording

C/EBPαp30 plays transcriptional regulatory roles distinct from C/EBPαp42

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cell Research 2007年第4期17卷 374-383页

作者： Chunxi Wang~1 Xiaotao Chen~1 Yanping Wang~1 Jialei Gong~1 Gengxi Hu ~1State Key Laboratory of Molecular biology institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,Graduate School of the chinese academy of sciences,shanghai 200031,china StateKeyLaboratoryofMolecularbiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesGraduateSchooloftheChineseAcademyofSciencesShanghai200031China

CCAAT/enhancer binding protein α(C/EBPα)is a transcriptional regulatory factor that inhibits cell proliferation,andalternative translational initiation produces two polypeptides,C/EBPcαp30 and C/EBPα*** expression profiling,it was revealed that C/EBPαp30 specifically inhibited a unique set of genes,including MPP11,p84N5 and SMYD2,which were not affected by C/EBPαp42 in both QSG-7701 hepatocyte cell line and QGY-7703 hepatoma ***-quantitative RT-PCR analysis independently confirmed these *** immunoprecipitation assay showed thatC/EBPαp30 bound to the promoters of these genes more strongly than C/EBPα*** clinical hepatoma samples inwhich C/EBPα was downregulated,all three genes specifically inhibited by C/EBPαp30 were ***,repression of MPP11,p84N5 and SMYD2 genes might not be directly involved in C/EBPαp30-mediated growth *** data suggest that C/EBPαp30 regulates a unique set of target genes and is more than a dominant-negativeregulator of C/EBPαp42.

关键词： C/EBPα expression pattern p84N5 MPP11 SMYD2

Possible Role of Histone H1 in the Regulation of Furin-dependent Proprotein Processing

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 173-180页

作者： Jinbo HAN~1 Jianxin GU~2 Chengwu CHI~(1,3*) 1 institute of biochemistry and cell biology,chinese academy of sciences,Graduate School of the chinese academy of sciences,shanghai 200031,china 2 Gene Research Center,Medical Center of Fudan University,shanghai 200032,china 3 institute of Protein Research,Tongji University,shanghai 200092,china institute of biochemistry and cell biology Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China.

Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitorsof furin,a mammalian proprotein ***,its role in the regulation of furin-dependent proproteinprocessing remains *** we report that histone H1 efficiently blocks furin-dependent pro-yonWillebrand factor（pro-vWF）processing in a dose-dependent *** and immunof-luorescence studies confirmed that histone H1 could interact with furin,and the interaction mainly took placeon the cell *** noted that histone H1 was released from cells undergoing necrosis and apoptosisinduced by *** findings suggested that histone H1 might be involved in extracellular and/or intracellu-lar furin regulation.

关键词： furin histone H1 pro-vWF proprotein processing

Structural Evidence for α-Synuclein Fibrils Using in Situ Atomic Force Microscopy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第2期37卷 113-118页

作者： Feng ZHANG Li-Na JI Lin TANG Jun HU Hong-Yu HU Hong-Jie XU Jian-Hua HE shanghai institute of Applied Physics,chinese academy of sciences.shanghai 201800.china Key Laboratory of Proteomics,institute of biochemistry and cell biology.shanghai institutes for biological sciences,chinese academy of sciences.shanghai 200031.china Bio-X Research Center.shanghai Jiaotong University.shanghai 200030.china shanghai institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Bio-X Research Center Shanghai Jiaotong University Shanghai 200030 China

Human α-synuclein is a presynaptic terminal protein and can form insoluble fibrils that arebelieved to play an important role in the pathogenesis of several neurodegenerative diseases such as Parkinson'sdisease,demen... 详细信息

关键词： results provide indirect evidence on how β-sheets assemble into α-synuclein fibrils on a nanometer scale.α-synuclein atomic force microscopy tapping mode contact mode guanidine hydrochloride