检索结果-南通市图书馆

Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells

Acta Pharmacologica Sinica 2004年第03期25卷 87-92页

作者： Lin CHANG2,3.Jian-xin XU4,Jing ZHAO5,Yong-zheng PANG2,3.Chao-shu TANG2,3.Yong-fen QI5,6 2Institute of Cardiovascular Diseases,the FirstHospital,peking University,beijing 10003. 3.nstitute of Cardiovascular Diseases,peking University,beijing 100083 4Instituteof Biophysics,Chinese Academy of sciences,beijing 100101 5department of physiology,health science Center,peking University,beijing 100083.china Institute of Cardiovascular Diseases the First Hospital Peking University Institute of Cardiovascular Diseases Peking University Institute of Biophysics Chinese Academy of Sciences Beijing 100101 department of physiology Health Science Center Pe

AIM: To observe protective effects of taurine on reactive oxygen species generation induced by homocysteine in rat vascular smooth muscle cells (VSMC). METHODS: Rat VSMC was incubated with various concentrations of homocysteine and taurine. The lactate dehydrogenase (LDH) activity which released into culture medium was elevated as an indicator for VSMC injury. The reactive oxygen species (ROS) — hydrogen peroxide (H2O2) and superoxide anion (O2ˉ· )were measured with luminol or lucigenin chemiluminescences method, and the mitochondria Mn-superoxide dismutase (Mn-SOD) and catalase (CAT) were also measured in treated VSMC. RESULTS: LDH leakage from cultured VSMC treated with homocystenie, was increased (P5, 10, and 20 mmol/L) significantly antago- nized 0.5 mmol/L homocysteine-induced H2O2generation in VSMC in a concentration dependent manner (P0.05 vs control). In this study, the superoxide anion in VSMC was not detectable by chemiluminent method. In addition, treatment of VSMC with taurine increased mitochondria Mn-SOD and CAT activity in a concentration dependent manner (P5), but homocysteine decreased mitochondria Mn-SOD and CAT activity (P<0.01 vs control). In addition, co-administration of taurine markedly ameliorated homocysteine-induced inhibition of Mn-SOD and CAT activity in VSMC (P<0.01 vs homocysteine alone group). CONCLUSION: Taurine antagonized the effects of homocysteine on ROS generation and anti-oxidant enzyme activities in rat VSMC in vitro.

关键词： homocysteine taurine vascular smooth muscle mitochondria reactive oxygen species

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

p3. MAPK mediates cardiovascular and behavioral responses induced by central IL-1β and footshock in conscious rats

引用

Acta Pharmacologica Sinica 2004年第3期25卷 12-16页

作者： Rui-mao ZHENG,Chang-jiang ZOU,Shi-gong ZHU2 department of physiology and Pathophysiology,{3. science Center,peking University,beijing 100083.china department of physiology and Pathophysiology Health Science Center Peking University Beijing 100083 China

AIM: To investigate the roles of p3. mitogen-activated protein kinase (p3. MAPK) in the cardiovascular and behavioral responses induced by intracerebral ventricular injection (icv) of interleukin-1β (IL-1β) or footshock. METHODS: We examined the effects of p3. MAPK on mean artery blood pressure (mABP), heart rate (HR), and motor activity (MA) during central administration of IL-1β, or footshock after icv SB203.80 (a specific inhibitor of the p3. MAPK) with Cardiovascular and Behavior Telemetry System in conscious SD rats. RESULTS: (1) IL-1β (icv) or footshock remarkably rise the mABP, and the maximal changes are (7.8±1.8) and (12.3.3.5) mmHg, respectively, which was abrogated by the pretreatment with p3. inhibitor SB203.80 intracerebroventricularly. (2) Compared with icv saline group, the motor activity was significantly decreased in SB203.80 group with maximal changes (-7.6±1.1) counts/min after footshock. CONCLUSION: p3. MAPK plays an important role in the pres- sor response induced by central administration of IL-1β or footshock and change of motor activity after footshock in conscious rats.

关键词： mitogen-activated protein kinase p3. interleukin-1 blood pressure motor activity

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide

引用

Acta Pharmacologica Sinica 2004年第6期25卷 131-136页

作者： Qi-zhen CAO, Zhi-bin LIN1 department of Pharmacology, health science Center, {3. University, beijing 100083, china department of Pharmacology Health Science Center Peking University

AIM: To investigate the antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide (GLPP). METHODS: Antitumor effect of GLPP was observed in tumor-bearing mice in vivo. At the same time, the effects of GLPP on proliferation of tumor cells and human umbilical cord vascular endothelial cell (HUVEC) were detected by MTTassay in vitro. Subsequently, spleen lymphocytes proliferation of nude mice was stimulated by LPS or ConA. To investigate the anti-angiogenic effect of GLPP, GLPP 80 μg per disc and GLPP-treated serum 10 μL per disc were added to the chick chorioallantoic membrane (CAM) respectively in vivo. RESULTS: GLPP 50, 100, and 200 mg/kg inhibited growth of Sarcoma 180 in BALB/c mice markedly by 3..2 %, 45.2 %, and 61.9 %, respectively. GLPP which was directly added to the cultured medium did not inhibit PG cell proliferation in vitro; but GLPP-treated serum 50, 100, 200 mg/kg potently inhibited PG cell proliferation by 22.5 %, 26.8 %, and 3..3.%, respectively; and reduced the xenograft (human lung carcinoma cell PG) in BALB/c nude mice greatly in vivo by 55.5 %, 46.0 %, and 46.8 %, respectively. Lymphocytes proliferation of nude mice could be stimulated by LPS 5 mg/Lbut not by ConA 2.5 mg/L, indicating that GLPP could not promote the T lymphocyte proliferation and neutral red phagocytosis of peritoneal macrophages of nude mice. The CAM assay showed that GLPP and GLPP-treated serum had anti-angiogenic effect. GLPP (1, 10, and 100 mg/L) inhibited HUVEC proliferation in vitro with the inhibitory rate of 9.4 %, 15.6 %, and 40.4 %, respectively. CONCLUSION: GLPP has antitumor and anti- angiogenic activity. The anti-angiogenesis of GLPP may be a new mechanism underlying its anti-tumor effects.

关键词： Ganoderma lucidum polysaccharides sarcoma 180 adenocarcinoma lung neoplasms lympho- cyte proliferation peritoneal macrophages angiogenesis nude mice

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Role of inositol 1,4,5-trisphosphate receptors in α1-adrenergic receptorinduced cardiomyocyte hypertrophy

引用

Acta Pharmacologica Sinica 2006年第7期27卷 895-900页

作者： Da-li LUO~(2,3.5) Jian GAO~2 Xiao-mei LAN~3.Gang WANG~4 Sheng WEI~4 Rui-ping XIAO~4 Qi-de HAN~3. department of Pharmacology,School of Chemical Biology & Pharmaceutical sciences,Capital University of Medical sciences,{3. 100069,china 3.Institute of Cardiovascular science at health science Center,peking University beijing 100083.china 4 Institute of Molecular Medicine and College of Life science,peking University,{3. 100871,china department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences Beijing 100069 China Institute of Cardiovascular science at health science Center Peking University Beijing 100083 China Institute of Molecular Medicine and College of Life science Peking University Beijing 100871 China

Aim:Intracellular Ca2+plays pivotal roles in diverse cellular functions,includinggene transcription that underlies cardiac remodeling during stress ***,the role of inositol 1,4,5-trisphosphate receptors（IP3.s）in the media-tion of cardiac intracellular Ca2+and hypertrophic growth remains *** with neonatal rat ventricular myocytes suggests that activation of IP3.smay be linked to α1adrenergic receptor（α1AR）increased stereotyped Ca2+sparkoccurrence and global Ca2+***,we hypothesized that Ca2+releasethrough IP3.s was necessary for α1AR-stimulated cardiac ***:We used myoinositol 1,4,5-trisphosphate hexakis（butyryloxymethyl）ester（IP3.M）,a membrane-permeant ester of IP3.to activate IP3.s directly,and Fluo 4/AM tomeasure intracellular Ca2+***:IP3.M(10μmol·L-1)mimicked theeffects of phenylephrine,a selective agonist of α1AR,in increments in local Ca2+spark release（especially in the perinuclear area）and global Ca2+*** importantly,IP3. inhibitors,2-aminoethoxydiphenyl borateand Xestospongin C,abolished the IP3.M-induced Ca2+responses,and signifi-cantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cellsize,[3.]leucine incorporation and atrial natriuretic factor gene expression,dur-ing sustained（48 h）phenylephrine ***:These results,therefore,provide cellular mechanisms that link IP3. signaling to α1AR-stimulatedgene expression and cardiomyocyte hypertrophy.

关键词： inositol 1,4,5-trisphosphate receptors Ca2+sparks α1 adrenergic stimulation cardiac hypertrophy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3.plus nicotine in rats

引用

Acta Pharmacologica Sinica 2006年第3期27卷 299-306页

作者： Sheng-ying WU~(2,6) Chun-shui PAN~3.Bin GENG~3.Jing ZHAO~3.Fang YU~3.Yong-zheng PANG~3.Chao-shu TANG~4 Yong-fen QI~(3.4,5) ~2Institute of Cardiovascular Disease,peking University First Hospital.beijing 10003. ~3.2} of {3. and Patho{3.,peking University health science Center,beijing 100083.china,~4Key Laboratory of Molecular Cardiovascular science,Ministry of Education,beijing 100083.china 2Institute of Cardiovascular Disease Peking University First Hospital Beijing 100034 China 3.0} of physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3.0} of physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3.0} of physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3.0} of physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3.0} of physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 4Key Laboratory of Molecular Cardiovascular science Ministry of Education Beijing 100083 China 4Key Laboratory of Molecular Cardiovascular science Ministry of Education Beijing 100083 China

Aim:To investigate the role of the endogenous cystathionine γ-synthase(CSE)/hydrogen sulfide(H2S)pathway in vascular calcification in ***:A ratvascular calcification model was established by administration of vitamin D3.plusnicotine(VDN).The amount of CSE and osteopontin(OPN)mRNA was deter-mined by using semi-quantitative reverse-transcription polymerase chain *** calcium content,45Ca2+accumulation and alkaline phosphatase(ALP)activitywere measured.H,S production and CSE activity were ***:yonKossa staining produced strong positive black/brown staining in areas amongthe elastic fibers of the medial layer in the calcified *** calcium content,45Ca2+accumulation and ALP activity in calcified arteries increased by 6.77-,1.42-,and 1.87-fold,respectively,compared with *** expression of the OPNgene was upregulated(P5Ca2+uptake and ALP activity in the VDN plus NaHSgroup was lower than that in the VDN *** content of calcium and45Ca2+accumulation and activity of ALP in the aorta were 3..8%,40.75% and 63.5% lowerin the low-dosage NariS group than in the VDN group,respectively(P5Ca2+and activity of ALP was 83.9%,3..8 % and 46.2% lower in the aorta in the high-dosage NariS group than in theVDN group,respectively(P<0.01).The expression of the OPN gene ***:The production of H,S,and CSE activity were de-creased and CSE gene expression was downregulated in rats with vascularcalcification.H2S can ameliorate vascular calcification,suggesting that the H2S/CSE pathway plays a regulatory role in the pathogenesis of vascular calcification.

关键词： hydrogen sulfide cystathionine-lyase vascular calcification mRNA

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Status of study on biological and toxicological effects of nanoscale materials

引用

science china Chemistry 2005年第5期48卷 385-394页

作者： WANG Bing1, FENG Weiyue1, ZHAO Yuliang1, XING Gengmei1, CHAI Zhifang1, WANG Haifang2 & JIA Guang3.1. Laboratory for Bio-Environmental health sciences of Nanoscale Materials (Nanosafety Lab), Institute of High Energy Physics, Chi-nese Academy of sciences, {3. 100049, china 2. College of Chemistry and Molecular Engineering, peking University, {3. 100871, china 3. department of Occupational and Environmental health sciences, School of Public health, peking University, {3. 100083, china 1. Laboratory for Bio-Environmental health sciences of Nanoscale Materials (Nanosafety Lab) Institute of High Energy Physics Chinese Academy of Sciences 100049 Beijing China 2. College of Chemistry and Molecular Engineering Peking University 100871 Beijing China 3. department of Occupational and Environmental health sciences School of Public Health Peking University 100083 Beijing China

Because the physical and chemical properties of nanosized materials mostly differ from the existing microsized materials, their potential impacts on human health and the envi-ronment will be topics under the serious discussions in press and in a number of international scientific journals. We analyze and summarize the existing data of the experimental study on the biological activities and adverse effects of nanoscale materials/particles including single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron powders. Though some biological behaviors of nanoscale materials observed cannot be understood on the basis of the current knowledge, as the existing data are mostly preliminary, it is too early to make some ex-clusive conclusions on biological activities (or the toxicity) of any of nanoscale materials. The experimental techniques, the current topics, and the future research directions for this new re-search field are also discussed.

关键词： nanomaterials, single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron, biological activity and toxicological effect.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Electrical Properties of Expanded Graphite Intercalation Compounds

引用

Journal of Materials science & Technology 2001年第3期17卷 371-374页

作者： Xiuyun CHUAN department of Geology, peking University, beijing 100871, {3. Daizhang CHEN and Xunruo ZHOU department of Materials science, {3. University of Geosciences, beijing 100083, {3. E-mail: cxyljhcj@*** peking Univ Dept Geol Beijing 100871 Peoples R China china Univ Geosci Dept Mat Sci Beijing 100083 Peoples R China

The intercalation compounds of CuCl2 were synthesized with expanded graphite, whose magnitude of the electrical conductivity is about 10(3.S(.)cm(-1). Their electrical conductivity is 3.similar to6 times as high as that of the expanded graphite, and about 10 times as high as that of GIC made of the non-expanded graphite. The microanalysis results of chemical compounds by X-ray energy spectrum scanning of TEM testified that the atomic ratio of chloride and cupric is nonstoichoimetric. The multivalence and exchange of electrovalence of the cupric ion was confirmed by the XPS-ESCA. Vacancy of chlorine anion increases the concentration of charge carrier. The special stage structure, made of graphite and chloride, produces a weak chemical bond belt and provides a carrier space in the direction of GIC layer. These factors develop the electrical properties.

关键词： In Ph Electrical Properties of Expanded Graphite Intercalation Compounds Graphite ESCA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Probability parametric imaging with DCE-MRI data to identify prostate cancer in the peripheral zone

引用

Progress in Natural science:Materials International 2006年第9期16卷 948-953页

作者： HUANG Xinrui, WANG Qiang, XU Yufeng, WANG Xiaoying and BAO Shanglian ( The Key Laboratory of Medical Physics and Engineering, peking University, beijing 100871, china The Medical School of peking University, beijing 100083, china 3. The First Hospital of peking University, beijing 10003., china) [a]The key Laboratory of Medical Physics and Engineering Peking University Beijing China [b]The Medical School of peking University Beijing China [c]The First Hospital of peking University Beijing China

To quantitatively analyze prostate dynamic contrast-enhanced magnetic resonance images (DCE-MRI), a retrospective study was carried out to construct a mathematical model to predict whether a lesion is cancerous or not. With known diagnosis of 52 re-gions-of-interest (10 malignant, 26 benign and 16 normal) in peripheral zone (PZ) of prostate from 18 patients undergoing needle biopsy and 8 normal volunteers, we combined feature parameters (k1, k2 and tp) from the linear-slope model with logistic regression to determine the statistical probability model. Prostate cancerous probability maps were derived using the model on each subject to examine the correlation between the model-based prediction and the known diagnosis. Compared the model prediction results of 52 regions-of-interest with the histopathologic results, the total agreement rate was 90.4 % ; the agreement rate was 60 % for the malignant and 97. 6 % for the nonmalignant. The diagnostic results only referencing the probability maps by two radiologists showed a high agreement in tissue characteristics and lesion location with the combining results from other diagnostic techniques such as PSA (prostate specific antigen) level and MRS (magnetic resonance spectroscopy) .

关键词： time-intensity curve prostate DCE-MRI logistic regression probability parametric image.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Platelet-derived growth factor receptor-β in myocyte was upregulated by angiotensin Ⅱ

引用

Chinese science Bulletin 2002年第12期47卷 1015-1018页

作者： BAI Hua, SUN Yinping, XING Dongqi, LIU Jie & WU Lilingdepartment of physiology and Pathophysiology, {3. University health science Center, beijing 100083, china department of Cardiology, General Post and Telecom Hospital, beijing 10003., china Departmen department of physiology and Pathophysiology Peking University Health Science Center Beijing China department of Cardiology General Post and Telecom Hospital Beijing China department of Pathophysiology Xinxiang Medical College Xinxiang China

To observe the regulation of platelet-derived growth factor (PDGF) receptor-p in myocyte stimulated by angiotensin Ⅱ (AngII) at both integrated and cellular levels and reveal the signal transduction mechanism in cell, two kidneys, one clip (2K1C) renal hypertension were performed by placing a sliver clip around the left renal artery. Blood pressure and the ratio of left ventricular weight to body weight were measured at 4 and 8 weeks after operation. The content of Angll in heart was detected by radioimmunology assay; the protein level of PDGF receptor-β in heart was measured by Western blot analysis. The alteration of PDGF receptor-β stimulated by Angll and several inhibitors was observed on cultured neonatal rat ventricular myocyte (NRVM). The content of Angll in heart of 2K1C renal hypertensive rat at 4 and 8 weeks after operation was increased. Compared with sham group, 4 and 8 weeks after operation, PDGF receptor-p in heart of 2K1C group was upregulated by 100.3. and 127.1% (P 5),

关键词： angiotensin II PDGF receptor-p myocyte phospholipase C extracellular signal-regulated kinase.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dehydration melting of solid amphibolite at 2.0 GPa: Effects of time and temperature

引用

science china Earth sciences 2005年第8期48卷 1120-1133页

作者： ZHOU Wenge1, XIE Hongsen1, LIU Yonggang1, ZHENG Xiaogang1, ZHAO Zhidan2 & ZHOU Hui3.1. Laboratory of Material in the Earth’s Interior, Institute of Geochemistry, Chinese Academy of sciences, Guiyang 550002, china 2. department of Geology, china University of Geosciences, beijing 100083, china 3. department of Geology, peking University, beijing 100871, china 1. Laboratory of Material in the Earth’s Interior Institute of Geochemistry Chinese Academy of Sciences 550002 Guiyang China 2. department of Geology China University of Geosciences 100083 Beijing China 3. department of Geology Peking University 100871 Beijing China

Two sets of dehydration-melting with a natural solid amphibolite, collected from North Himalayan structure zone, Tibet, have been carried out in multi-anvil apparatus at 2.0 GPa and 800―1000℃, for 12―200 h. One is keeping the pressure at 2.0 GPa and the annealing time of 12 h, changing the temperature (800―1000℃). The other is keeping the pressure at 2.0 GPa and temperature at 850℃, varying the annealing time (12―200 h). The products are inspected with microscope and electron probe. The results indicate that at 2.0 GPa, annealing time of 12 h, garnets, melts and clinopyroxenes occur in amphibolite gradually with increasing temperature and the chemical compositions of melt vary from tonalite to granodiorite, and then to tonalite. However, at 2.0 GPa and 850℃, with the annealing time increasing, the garnets, melts and cli-nopyroxenes also occur in amphibolite gradually and the chemical compositions of melt vary from tonalite to granodiorite. In both cases, melts interconnect with each other when the contents of melt are over the 5 vol.%. the viscosities of the melt produced in amphibolite at temperature higher than 850℃ are on a level with 104 Pa·s. The interconnected melt with such a viscosity may segregate from the source rock and form the magma over reasonable geological time. Therefore, it is believed that at the lower part of the overthickened crust, the tonlitic and grano-dioritic magma may be generated through the dehydration melting of amphibolite.

关键词： solid amphibolite, high pressure, high temperature, time, partial melting.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：