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Mechanism of Calcitonin Gene-related Peptide Modulating Apoptosis of Vascular Smooth Muscle Cells

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Mechanism of Calcitonin Gene-related Peptide Modulating Apop...

Predicting Polymerase Ⅱ Core Promoters by Cooperating Transcription Factor Binding Sites in Eukaryotic Genes

2002全国青年药理学英文学术会议

作者： WANG Wang, WANG Xian (department of physiology, health science center, peking university, beijing, 100083)

AIM: Atherosclerosis (AS) is one of the major threatens of the health and life of human being. Accumulating data have supported the idea that the pathogenesis of AS includes a set of inflammatory and immune responses. The migration, proliferation and apoptosis of smooth muscle cells (SMC) also play an important role. Calcitonin gene-related peptide (CGRP) is a neuropeptide with potent vasodilatory effect. CGRP and its receptors are widely expressed throughout the cardiovascular system. Recently, more and more vasodilators have been found to have antiproliferative effects on SMC. Our previous data

关键词： Calcitonin Gene-related Peptide /Vascular Smooth Muscle Cells /Apoptosis

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 250-258页

作者： Xiao-Tu MA, Min-Ping QIAN*, and Hai-Xu TANG1 School of Mathematical sciences, peking university and center for Theoretical Biology, peking university, beijing 100871, china 1department of Computer science and Engineering, university of California, San Diego, La Jolla CA 92093-0114, USA School of Mathematical sciences Peking University and Center for Theoretical Biology Peking UniversityBeijing 100871 China department of Computer science and Engineering University of CaliforniaSan Diego La Jolla CA 92093-0114 USA

Several discriminate functions for predicting core promoters that based on the potential cooperation between transcription factor binding sites (TFBSs) are discussed. It is demonstrated that the promoter predicting accuracy is improved when the cooperation among TFBSs is taken into consideration. The core promoter region of a newly discovered gene CKLFSF1 is predicted to locate more than 1.5 kb far away from the 5' end of the transcript and in the last intron of its upstream gene, which is experimentally confirmed later. The core promoters of 3402 human RefSeq sequences, obtained by extending the mRNAs in human genome sequences, are predicted by our algorithm, and there are about 60% of the predicted core promoters locating within the ± 500 bp region relative to the annotated transcription start site.

关键词： promoter transcription factor binding site transcription start site gene finding

Inhibition of the activating signals in NK92 cells by recombinant GST-sHLAG1α chain

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Cell Research 2004年第2期14卷 155-160页

作者： Ai Yu YAO~(1,2), Hai Yang TANG~1, Yun WANG~1, Mei Fu FENG~(1,*), Rou Li ZHOU~(2,*) ~1State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of science, beijing 100080, china ~2department of Cell Biology and Medical Genetics, School of Basic Medical sciences, health science center, peking university, beijing 100083, china. State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Science Beijing 100080 China department of Cell Biology and Medical Genetics School of Basic Medical Sciences Health Science Center Peking University Beijing 100083 China

The soluble HLA-G1 (sHLA-G1) isoform was found to be secreted by trophoblast cells at the matemo-fetal interface, which suggests that it may act as an immunomodulator during pregnancy. In this paper, we reported that GST-sHLA-G1α chain could bind to its receptor ILT-2 on NK92 cells and then the latter recruited Src homology 2 domaincontaining tyrosine phosphatase-1 (SHP-1), which consequently dephosphorylated some important protein tyrosine kinases and blocked the activation of downstream molecules such as MEK and ERK so that the cytotoxicity of natural killer (NK) cells was inhibited. These results indicated that GST-sHLA-G1α chain might be exploited in new immunotherapy strategies aiming at inducing immunotolerance during allograft, xenograft and autoimmune situations. In addition, we found that modification of O-linked β-N-acetylglucosamine (O-GlcNAc) was involved in NK cells’ activating and inhibitory signals. This may provide a novel molecular target for inducing immunotolerance but needs furthe

关键词： NK92 GST-sHLA-G1α chain signal transduction SHP-1 O-G1cNAc.

Receptor subtype involved in α-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes

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Acta Pharmacologica Sinica 2007年第7期 968-974页

作者： Da-li LUO~(2,3,4),Jian GAO~2,Lin-lin FAN~2,Yu TANG~2,You-yi ZHANG~3,Qi-de HAN~3~2 department of Pharmacology,School of Chemical Biology and Pharmaceutical sciences,Capital university of Medical sciences,beijing100069,china ~3 Institute of Cardiovascular science at the health science center,peking university,beijing 100083,china

Aim:The enhancement of intracellular Ca2+signaling in response to α1-adrener-gic receptor （α1-AR） stimulation is an essential signal transduction event in theregulation of cardiac functions,such as cardiac growth,cardiac contraction,andcardiac adaptation to various *** present study was intended todetermine the role（s） of the α1-AR subtype（s） in mediating this ***:We evaluated the effects of subtype-specific agonists and antagonists of the α1-AR on the intracellular Ca2+signaling of neonatal rat ventricular myocytes usinga confocal ***:After being cultured for 48 h,the myocytesexhibited spontaneous local Ca2+release,sparks,and global Ca2+*** of the α1-AR with phenylephrine,a selective agonist of the α1-AR,dose-dependently increased the frequency of Ca2+transients with an EC50valueof 2.3 μmol/*** the α1A-AR subtype with 5-methylurapidil （5-Mu） inhi-bited the stimulatory effect of phenylephrine with an IC50value of 6.7 nmol/***,blockade of the α1B-AR and α1D-AR subtypes with chloroethylclonidineand BMY 7378,respectively,did not affect the phenylephrine ***,thelocal Ca2+spark numbers were also increased by the activation of the α1-AR,andthis effect could be abolished selectively by *** importantly,A61603,anovel selective α1A-AR agonist,mimicked the effects of phenylephrine,but withmore potency (EC50value=6.9 nmol/L) in the potentiation of Ca2+transients,andblockade of the α1A-AR by 5-Mu caused abolishment of its ***:These results indicate that α1-adrenergic stimulation of intracellular Ca2+activityis mediated selectively by the α1A-AR.

关键词： α1-adrenergic receptor subtype cardiomyocyte Ca2+ signaling A61603

Frontal genesis of moisture atmosphere during the early 2008 persistent freezing-rain event in southern china

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science china Earth sciences 2010年第3期53卷 454-464页

作者： QIAN WeiHong1 & FU JiaoLan1,2 1 department of Atmospheric sciences, School of Physics, peking university, beijing 100871, china 2 National Meteorological center of CMA, beijing, 100081, china 1. department of Atmospheric sciences School of Physics Peking University Beijing 100871 China2. National Meteorological Center of CMA Beijing 100081 China

From January 10 to February 2, 2008, a severe and persistent freezing-rain event occurred in southern and southwestern china. Here we use an observational analysis to compare the persistent freezing-rain event in the early 2008 with the winter precipita- tion in the late 2007 over south of the Yangtze River (Jiangnan). The persistent freezing-rain event was directly linked to the activity of quasi-stationary front. The gradient of equivalent temperature (ET) can well indicate the frontal genesis of moist atmosphere (moisture front) and its activity as well as its relationship with precipitation belt. The precipitation types (snow and freezing rains) are related to the vertical structure of moisture front. The inversion profile of ET vertical distribution is a typical synoptic condition that caused the freezing-rain event. The horizontal gradient of ET with a criterion of 10℃ / 100 km, which reflects the accumulation and release of atmospheric energy, can be applied to predict the precipitation 5-10 days in advance.

关键词： freezing-rain event frontal genesis extended-range weather forecast winter Jiangnan

The variation in the magnetic fluctuation anisotropy across the front boundaries of magnetic clouds and its geomagnetic response

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Chinese science Bulletin 2002年第10期47卷 863-867页

作者： LIU Rui & WEI Fengsidepartment of Geophysics, peking university, beijing 100871, china Lab for Space Weather, center for Space science and Applied Research, Chinese Academy of sciences, beijing 100080, china department of Geophysics Peking University Beijing China Lab for Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China

A statistical study of magnetic fluctuations near the front boundaries of magnetic clouds is approached with the method of Minimum Variance Analysis, based on the data of ImpS and Wind spacecraft. New discoveries are that (1) fluctuation anisotropy tends to increase across the front boundaries of magnetic clouds; (2) there is a good correlation between the fluctuation anisotropy and the geomagnetic activity indices; and (3) in some cases, although there is southward field component immediately after the front boundary, Kp index descends (or Dst index ascends) with a corresponding decrease of the fluctuation anisotropy; in other cases with no distinct southward field component, Kp index ascends (or Dst index descends) with a corresponding increase of the fluctuation anisotropy. Thus we suggest that the fluctuation anisotropy might be a useful indicator in diagnosing the magnetic activities of magnetic clouds.

关键词： front boundary of magnetic clouds geomagnetic activity fluctuation anisotropy.

Regulation of angiotensin Ⅱ on Gαq/11 protein of vascular smooth muscle cell and its underlying mechanism

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Chinese science Bulletin 2002年第16期47卷 1369-1372页

作者： XING Dongqi, BAI Hua, ZHAO Yali & WU Lilingdepartment of physiology and Pathophysiology, peking university health science center, beijing 100083, china

To study the regulation of angiotensin Ⅱ (Ang Ⅱ) on Gαq/11 protein of vascular smooth muscle cell (VSMC) and its underlying mechanism, the protein synthesis was detected by [3H]-leucine incorporation. Gαq/11 expression was measured by Western blot in cultured VSMC of rat aorta. The results showed that the level of Gαq/11 was down-regulated after stimulated by Ang Ⅱ for 1-6 h, while it was upregulated significantly by 12-24 h stimulation (P 59 did not have this effect. These data indicated that Ang Ⅱ contributed to VSMC hypertrophy by regulating the level of Gαq/11, and this effect was mediated mainly through AT1 receptor-PLC signal transduction pathway.

关键词： angiotensin II vascular smooth muscle cell G protein hypertrophy signal transduction.

Response synchronization: New progress in brain-visual information processing

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Chinese science Bulletin 1999年第16期44卷 1447-1458页

作者： ZHAO Songnian, XIONG Xiaoyun, YAO Guozheng and GUO Aike1. State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry , Institute of Atmospheric Physics , Chinese Academy of sciences , beijing 100029, china 2. Commission of the National Natural science Foundation of china , beijing 100083, china 3. The center of Information science , peking university, beijing 100871, china 4. Open Laboratory of Visual Information Processing , Institute of Biophysics , Chinese Academy of sciences , beijing 100101, china State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China Commission of the National Natural science Foundation of china Beijing China The center of Information science Peking University Beijing China Open Laboratory of Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing China

Studies on brain-vision have entered a new stage, after the stimulus-induced y synchronized oscillation of neuron group activities have been discovered in cat primary visual cortex. Naturally, how to understand the visual system became a very important methodological problem. From aspects such as information processing, nonlinear dynamics and neuronal network, some primary problems in vision studies have been deeply discussed, which are

关键词： neuron group (neural population) synchro-oscillation computational theory of vision response synchroniza-tion.

The theoretical research of basic function method in incompressible viscous flow and its simulations in three-dimensional aneurysms

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science china(Physics,Mechanics & Astronomy) 2009年第6期52卷 909-918页

作者： SHEN Fang1 & WU WangYi2 1 State Key Laboratory for Space Weather, center for Space science and Applied Research, Chinese Academy of sciences, beijing 100190, china 2 State Key Laboratory of Turbulence and Complex Systems & department of Mechanics and Engineering science, peking university, beijing 100871, china 1. State Key Laboratory for Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing 100190 China 2. State Key Laboratory of Turbulence and Complex Systems & department of Mechanics and Engineering science Peking University Beijing 100871 China

Basic function method is developed to treat the incompressible viscous flow. Artificial compressibility coefficient, the technique of flux splitting method and the combination of central and upwind schemes are applied to construct the basic function scheme of trigonometric function type for solving three-dimensional incompressible Navier-Stokes equations numerically. To prove the method, flows in finite-length-pipe are calculated, the velocity and pressure distribution of which solved by our method quite coincide with the exact solutions of Poiseuille flow except in the areas of entrance and exit. After the method is proved elementary, the hemodynamics in two-and three-dimensional aneurysms is researched numerically by using the basic function method of trigonometric function type and unstructured grids generation technique. The distributions of velocity, pressure and shear force in steady flow of aneurysms are calculated, and the influence of the shape of the aneurysms on the hemodynamics is studied.

关键词： basic function unstructured grid aneurysm viscous incompressible flow artificial compressibility flux splitting