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Hyaluronan Promotes Tumor Lymphangiogenesis and Intralymphantic Tumor Growth in Xenografts

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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 601-606页

作者： Li-Xia GUO Ke ZOU Ji-Hang JW Hong XIE Laboratory of Biotherapy,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological sciences,chinese academy of sciences,graduate {5. of the chinese academy of sciences,Shanghai 200031,china Laboratory of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

Hyaluronan (HA),a high molecular weight glycosaminoglycan in the extracellular matrix,hasbeen implicated in the promotion of malignant phenotypes,including tumor ***,little isknown about the effect of HA on tumor-associated *** this study,mouse hepatocellularcarcinoma Hca-F cells combined with or without HA were injected subcutaneously into C3H/Hej mice,thenangiogenesis and lymphangiogenesis of implanted tumors were examined by immunostaining for platelet-endothelial cell adhesion molecule-1 and lymphatic vascular endothelial hyaluronan receptor-1 ***,we found HA promotes tumor lymphangiogenesis and the occurrence of intratumoral lymphaticvessels,but has little effect on tumor ***,HA also promotes intralymphatic tumorgrowth,although it is not sufficient to potentiate lymphatic *** results suggest that HA,which is elevated in most malignant tumor stroma,may also play a role in tumor progression by promotinglymphangiogenesis.

关键词： hyaluronan tumor lymphangiogenesis intralymphatic tumor growth

An improved method for evaluating the seasonal variability of total suspended sediment flux field in the Yellow and East china Seas

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International Journal of Sediment Research 2011年第1期26卷 1-14页

作者： PANG Chongguang~1,YU Wei~2,YANG Yang~3,and HAN Danxiu~4 Institute of Oceanology,chinese academy of sciences,Qingdao 266071,china, 2 Institute of Oceanology,chinese academy of sciences,Qingdao 266071,china,graduate school of chinese academy of sciences,Beijing {13.,china 3 Institute of Oceanology,chinese academy of sciences,Qingdao 266071,china,graduate school of chinese academy of sciences,Beijing {13.,china 4 china University of Petroleum,Beijing 102249,china Doctor Researcher Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of Chinese Academy of Sciences Beijing 100039 China china University of Petroleum Beijing 102249 China

The suspended sediment flux field in the Yellow and East china Seas(YECS) displays its seasonal variability.A new method is introduced in this paper to obtain the flux field via retrieval of ocean color remote sensing data,statistical analysis of historical suspended sediment concentration data,and numerical simulation of three-dimensional(3D) flow *** components of the sediment flux field include(i) surface suspended sediment concentration inverted from ocean color remote sensing data;(ii) vertical distribution of suspended sediment concentration obtained by statistical analysis of historical observation data;and(iii) 3D flow field modeled by a numerical *** the improved method,the 3D suspended sediment flux field in the YECS has been *** comparison with the suspended sediment flux field solely based on the numerical simulation of a suspended sediment transport model,the suspended sediment flux field obtained by the improved method is found to be more *** 3D suspended sediment flux field from ocean colour remote sensing and in situ observation are more closer to the ***,by quantitatively analyzing the newly obtained suspended sediment flux field,the quantity of sediment erosion and deposition within the different regions can be *** sediment exchange between the Yellow Sea and the East china Sea can be *** mechanism of suspended sediment transport in the YECS can be better *** particular,it is suggested that the long-term transport of suspended sediment is controlled mainly by the circulation pattern,especially the current in winter.

关键词： Suspended sediment flux field Seasonal variability Yellow and East china Seas Regression equation Numerical simulation

Contributions of phosphatase and microbial activity to internal phosphorus loading and their relation to lake eutrophication

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Science china Earth sciences 2006年第Z1期49卷 102-113页

作者： SONG Chunlei, CAO Xiuyun, LI Jianqiu, LI Qingman, CHEN Guoyuan & ZHOU Yiyong Institute of Hydrobiology, chinese academy of sciences, Wuhan 430072, china graduate {5. of the chinese academy of sciences, Beijing 10003., china Institute of Hydrobiology Chinese Academy of Sciences Wuhan China graduate school of the chinese academy of sciences Beijing China

Phosphatase may accelerate the process of lake eutrophication through improving phosphorus bioavailability. This mechanism was studied in three chinese eutrophic shallow lakes (Lake Taihu, Lake Longyang and Lake Lianhua). Phosphatase activity was related to the concentration of soluble reactive phosphorus (SRP) and chlorophyll a. Stability of dissolved phosphatase in reverse micelles may be attributed to molecular size, conformation and active residues of the *** the site with Microcystis bloomed in Lake Taihu, dissolved phosphatase activity was higher and more stable in micelles, SRP concentrations were lower in interstitial water, the contents of different forms of phosphorus and the amounts of aerobic bacteria were lower while respiration efficiency was higher in sediments. Phosphobacteria, both inorganic and organic and other microorganisms were abundant in surface water but rare in sediments. Therefore, internal phosphorus may substantially flux into water column by enzymatic hydrolysis and anaerobic release, together with mobility of bacteria,thereby initiating the bloom. In short, biological mechanism may act in concert with physical and chemical factors to drive the internal phosphorus release and accelerate lake eutrophication.

关键词： dissolved phosphatase, reverse micelle, internal loading, phosphorus form of sediment, microbiology, eutrophication, Microcystis bloom.

Expression and purification of lipoprotein-associated phospholipase A2,a key enzyme involved in atherosclerosis

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Acta Pharmacologica Sinica 2006年第6期27卷 679-684页

作者： Fu-jun ZHANG~(2,4)Mao-jun CAI~3 Jing-kang SHEN~3 Yi-ping WANG~(2,5.2 State Key Laboratory of Drug Research.3 Department of Medicinal Chemistry,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological sciences,chinese academy of sciences.Shanghai 201203,china 4 {5. school of the chinese academy of sciences.chinese academy of sciences,Shanghai 201203.china State Key Laboratory of Drug Research Chinese Academy of Sciences Shanghai China graduate school of the chinese academy of sciences Chinese Academy of Sciences Shanghai China Department of Medicinal Chemistry Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To express and purify lipoprotein-associated phospholipase A2（Lp-PLA2）,and to establish a screening model for Lp-PLA2inhibitors using the expressed Lp-PLA2,Methods:We cloned the full-length cDNA of Lp-PLA2 from differentiatedTHP-I cells,and subcloned the cDNA into the baculovirus transfer *** addition,we introduced an N-terminal Kozak sequence for high-level translation initiation and a C-terminal sequence of 6 histidine residues forpurification,The fusion enzyme was expressed in Sf9 insect cells and purified byNi2+affinity *** Lp-PLA2activity was measured us-ing[3H]PAF as a substrate,and we examined the enzyme activity of recombinantLp-PLA2pretreated with the known Lp-PLA2inhibitor ***:Wesuccessfully cloned the full-length Lp-PLA2gene from differentiated THP-1 *** fusion enzyme was expressed in Sf9 insect cells at a high level and purifiedefficiently through a 2-step *** recombinant Lp-PLA2activity wasmeasured using[3H]PAF as a substrate,and proved to be enzymatically ***-PLA2inhibitor SB435.95.produced a good inhibition curve for inhibition ofrecombinant Lp-PLA2with an IC5.of 5.±1μmol/***:We expressed andpurified Lp-PLA2at a high level in insect cell-baculovirus expression *** ratio was much greater than that obtained from human plasma and we estab-lished a screening model for Lp-PLA2inhibitors using the expressed Lp-PLA2.

关键词： lipoprotein-associated phospholipase A2 atherosclerosis coronary heart diseases cloning baculovirus purification

An Efficient High-Resolution Shock-Capturing Scheme for Multi-Dimensional Flows I. Hydrodynamics

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chinese Journal of Astronomy and Astrophysics 2006年第6期6卷 680-688页

作者： Cong Yu National Astronomical Observatories / Yunnan Astronomical Observatory, chinese academy of sciences, Kunming 65.011 graduate school of chinese academy of sciences, Beijing 100049 National Astronomical Observatories / Yunnan Astronomical Observatory Chinese Academy of Sciences Kunming 650011 graduate school of chinese academy of sciences Beijing 100049

Many problems at the forefront of theoretical astrophysics require a treatment of dynamical fluid behavior. We present an efficient high-resolution shock-capturing hydrodynamic scheme designed to study such phenomena. We have implemented a weighted, essentially non-oscillatory （WENO） scheme to fifth order accuracy in space, lILLE approximate Riemann solver is used for the flux computation at cell interface, which does not require spectral decomposition into characteristic waves and so is computationally friendly. For time integration we apply a third order total variation diminishing （TVD） Runge-Kutta scheme. Extensive testing and comparison with schemes that *** characteristic decomposition are carried out demonstrating the ability of our scheme to address challenging open questions in astrophysics.

关键词： accretion - hydrodynamics- method numerical - shock waves

Twistor bundle theory and its application

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Science china Mathematics 2004年第4期47卷 605-616页

作者： PENG Chia-Kuei & TANG ZizhouDepartment of Mathematics, graduate school of chinese academy of sciences, Beijing {5., china Department of Mathematical sciences, Tsinghua University, Beijing 100084, china Department of Mathematics Graduate School of Chinese Academy of Sciences Beijing China Department of Mathematical sciences Tsinghua University Beijing China

Over an oriented even dimensional Riemannian manifold(M 2m ,ds2 ), in terms of the Levi-Civita connection form Ω and the canonical form Θ on the bundle of positive orthonormal frames, we give a detailed description of the twistor bundle Гm = SO(2m)/U(m)? J +(@#@ M,ds2 ) →M. The integrability on an almost complex structureJ compatible with the metric and the orientation, is shown to be equivalent to the fact that the corresponding cross section of the twistor bundle is holomorphic with respect toJ and the canonical almost complex structureJ 1 onJ +(M,ds2 ), by using moving frame theory. Moreover, for various metrics and a fixed orientation onM, a canonical bundle isomorphism is established. As a consequence, we generalize a celebrated theorem of LeBrun.

关键词： almost complex structure, integrability, twistor bundle.

Expression of Monomeric Insulin Precursor in Pichia pastoris and Its Conversion into Monomeric B27 Lys Destripeptide Insulin by Tryptic Hydrolysis

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3) Jian FEI~2 Da-Fu CUI~1 You-Shang ZHANG~1 ~1Institute of Biochemistry and Cell Biology,and ~2Model Organism Research Center,Shanghai Institutes for Biologicol sciences, chinese academy of sciences,Shanghai 200031,china ~3{5. school of the chinese academy of sciences,Shanghai 200031,china Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China graduate school of the chinese academy of sciences Shanghai 200031 China Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Monomeric B27 Lys destripeptide insulin (B27 Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B27 Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B27 Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B27 Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 200 mg per liter of culture,and the overall yield ofpurified B27 Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B27 Lys DTrI asdetermined by the mouse convulsion assay was 21 U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B27 Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

Non-Gaussianity of Racetrack Inflation Models

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Communications in Theoretical Physics 2007年第7期48卷 189-192页

作者： SUN Cheng-Yit ZHANG De-Hai Department of Physics,The graduate school of The chinese academy of sciences,Beijing 100049,{5. Department of Physics The Graduate School of The Chinese Academy of Sciences Beijing 100049 China

In this paper,we use the result in[*** and ***,arXiv:astro-ph/05.0709]to calculate thenon-Gaussianity of the racetrack models in[***-Pillado,et al.,JHEP 0411(2004)063;arXiv:hep-th/0406230]and[***-Pillado,et al.,arXiv:hep-th/0603129].The two models give different *** of themare ***,we find that,for multi-field inflationary models with the non-trivial metric of the field space,the condition of the slow-roll cannot guarantee small non-Gaussianities.

关键词： non-Gaussianity non-linear parameter racetrack inflation

Identification of the Angel -related elements in cyprinid fishes and their phylogenetic implications

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Progress in Natural Science:Materials International 2007年第2期17卷 168-171页

作者： WANG Li, HE Shunping and CHEN Yiyu ( Institute of Hydrobiology, chinese academy of sciences, Wuhan 430072, china graduate {5. of the chinese academy of sciences, Beijing 10003., china National Natural Science Foundation of china, Beijing 100085. china) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China graduate school of the chinese academy of sciences Beijing 100039 China National Natural Science Foundation of china Beijing 100085 China

Angel-related element belongs to the family of miniature inverted-repeat transposable elements (MITEs). In this paper we report the identification of an Angel- related element in the series Leuciscini of cyprinid fishes, which is located in the second intron of the growth hormone (GH) gene. We have also found that this element is absent in orthologous locus in the series Barbini of cyprinid fishes, that provides new evidence for the monophyly of the series Leuciscini. The insertion of Angel -related element into the GH gene might take place in the common ancestor of the series Leuciscini after its divergence from the series Barbini. The high sequence divergence and relatively broad species distribution of Angel-related elements implies that they might be ancient transposons which appeared about 26 million years ago.

关键词： Angel -related elements the series Leuciscini monophyly.