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变性梯度凝胶电泳在堆肥微生物研究中的应用

微生物学通报 2004年第5期31卷 116-119页

作者：崔宗均宫小燕李国学中国农业大学农业与生物技术学院北京100094 中国农业大学资源与环境学院北京100094

对当前堆肥中微生物种群分布及其对有机物分解作用的研究进行分析 ,论述了分子生物技术中的变性梯度凝胶电泳 (DGGE)的特点。将DGGE与PCR扩增技术相结合 ,可用于研究自然菌种堆肥和人工培养驯化菌种堆肥过程中微生物的演替规律 ,为研究... 详细信息

对当前堆肥中微生物种群分布及其对有机物分解作用的研究进行分析 ,论述了分子生物技术中的变性梯度凝胶电泳 (DGGE)的特点。将DGGE与PCR扩增技术相结合 ,可用于研究自然菌种堆肥和人工培养驯化菌种堆肥过程中微生物的演替规律 ,为研究和筛选堆肥中的微生物提供更加有效、快速的信息 ,促进堆肥技术的发展。

关键词：变性梯度凝胶电泳堆肥微生物演替

Mapping a Female Sterile Gene locus in Wheat (Triticum Aestivum L.)

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Mapping a Female Sterile Gene locus in Wheat (Triticum Aesti...

中国遗传学会第八次代表大会暨学术讨论会

作者： Dou B.~1 Hou B.~1 Zhu X.~1 Yang J.~1 Wang F.~2 Xu H~3 Ni Z~4 Sun Q~4 1.Institute of Plant Biotechnology,Jiangsu Key Laboratory of Eco-agricultural Biotechnology around Hongze Lake,Huaiyin Teachers college,Huai’an city,223301,china, 2.Agronomy college,Xinjiang agricultural {5.,Urumqi,223301,china 3.college of Agriculture & Biotechnology,Zhejiang {5.,Hangzhou,310029,china 4.Department of Plant Genetics and Breeding,china agricultural {5.,beijing 100094, china

Many kinds of male sterile lines were found in past decades which let a hermaphrodite species be a gynodioecy,whereas a female sterile line let the gynodioecy be a ***,a new female sterile mutant was found in wheat at Urumqi(china)with one feature of automatically restoring its fertility in later sowing *** understand the genetic mechanism of the female *** F2 population derived from a hybrid cross between an elite variety(8901,normal fertility)and the female sterile line(XND 126)was planted at Haian experimental station in autumn of 2004,two same component F2 populations were planted at Haian and Urumqi experimental station separately by tillers split in autumn of *** female fertility was showed by seed set under fully pollinated condition,SSR marker was used to check the population genotype,data were analyzed by the gene mining software Mapmaker 3.0 and *** linkage disequilibrium(r2)between gene locus and SSR marker was calculated in a population of female sterile line and 260 varieties after its’structure *** distribution of the female fertility in F2 was bimodal and skewed toward the normal parent of *** on the information of 8 molecular markers in two linkage groups and of phenotype data in 2005.a major gene locus taf1 (triticum aestivum female fertility)was identified,which was tightly linked with marker Xbarc95.on chromosome 2DS,with genetic effects accounting for 21.5.% and 13.64% of phenotype variation showed by modified domestic and international method *** 2007 results,23 markers in 5.linkage groups were involved in analysis of mining the gene locus taf1,it was more tightly linked with marker Xgdm35.accounting for 41.20% and 30.77% of phenotype variation showed also by the method *** on the result of linkage analysis,the linkage disequilibrium was studied,the result was showed that,high degree of LD were found between the gene locus taf1 and marker Xbarc35.Xbarc95.within a

关键词： wheat female sterility QTL mapping Linkage analysis association analysis

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北京山区农业气候资源系统的模糊综合评判

山地学报 2005年第4期23卷 4507-4512页

作者：田志会郭文利赵新平王志华郑大玮中国农业大学资源与环境学院北京市气候中心中国农业大学资源与环境学院北京100094 北京农学院园林系北京102206 北京100089

为了定量分析北京山区农业气候资源,利用山区1 km2小网格的气候资料,应用农业气候适宜度理论、模糊数学和因子分析等方法,通过5.农业气候指标与山区栽培植物生长发育条件之间隶属函数的建立及其权重的确定,分别计算山区各网格点的农业... 详细信息

为了定量分析北京山区农业气候资源,利用山区1 km2小网格的气候资料,应用农业气候适宜度理论、模糊数学和因子分析等方法,通过5.农业气候指标与山区栽培植物生长发育条件之间隶属函数的建立及其权重的确定,分别计算山区各网格点的农业气候资源指数、效能指数和利用系数,并在GIS的支持下建立了它们的栅格图。结果表明:北京山区以平谷、密云、怀柔南部等东北部地区的农业气候资源系统较为优良,无论是气候资源总量还是其匹配状况以及利用率都是本地区最高的;与之相反,西北部和海拔较高的山区,不仅农业气候资源总量贫乏,而且光、热、水资源的匹配程度较差,导致农作物对资源的利用率较低;此外,房山东南部和昌平南部等地区气候资源总量较为丰富,但受水资源的限制,光、热、水资源的匹配程度较差,致使当地气候资源利用率较低。

关键词：农业气候适宜度 GIS 小网格模糊综合评判山区

Research and Design of Digital Video Player Based on AML8618

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Research and Design of Digital Video Player Based on AML8618

第三届国际计算机及计算技术农业应用学术会议

作者： Wang Jin Wanlin Gao Qing Wang Hongbiao Jiang college of information and electrical engineering china {5. university Qi Zhang Miyun Electric Power Company Wenxue Cao Control House of Transferring Water from East to West Division of Transferring Water from East to West Administration

＜正＞In this paper,a novel design of digital video player is proposed based on *** the usage of advanced multimedia processor AML8616,digital video player can play multimedia files in many formats, with many output interfaces,and transmit multimedia files through Ethernet *** paper presents the design of hardware according to the characteristics of AML8618 and workflow of the *** on the hardware characteristics,the software is designed based on the embedded Linux operating *** novelties of our proposed work are its powerfulness and simplicity of *** design scheme would be a good reference for the design of digital video player,and it would promote the development of future digital video players.

关键词： Digital Video Player hardware frame embedded system Circuit Design

LANDFORM MORPHOLOGIC RELATIONS BETWEEN INTER-GULLIED AND GULLIED LAND AREAS——A CASE STUDY OF THE WANGJIAGOU WATERSHED IN THE WEST OF SHANXI PROVINCE

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International Journal of Sediment Research 2006年第1期21卷 62-73页

作者： Hao CHEN1, Hai-yan FANG2, Qiang-guo CAI 3, Guang-lin LIANG4 and Zong-qi YUE5.Associate Professor, 2Doctor, 3Professor, 4Postgraduate for master, Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, beijing, 100101, china 1Professor, college of Resource and Environment, Southwest agricultural university, Chongqing, 400716, china 2 Doctor, 4 Postgraduate for master, Graduate university of Chinese Academy of Sciences, beijing, 100049, china 5.Associate Professor, Department of Civil engineering, Hong Kong university, Hong Kong, china Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Department of Civil engineering Hong Kong University Hong Kong China

Previous studies on erosional relations between inter-gullied and gullied lands have mainly concentrated on exploration of water and sediment relations, with few quantitative studies of evolution relations between inter-gullied land and gullied landform erosion in small catchments. This paper, using information from a 1:5.00 digital orthophotomap (DOM), digital elevation model (DEM), and topographic map of the same period and of the same scale, quantitatively analyzes the impact and interactions of inter-gullied and gullied land geomorphologic characteristics on the dissected extent of a catchment using orthogonal polynomial regression analysis. Results indicate that gullies play a leading role in the catchment morphologic evolution and extent of cracked ground surface. When inter-gullied land areas are the same, a 0.1 km2 gullied land area has the maximum effect on the erosive evolution intensity of the catchment cracked degree. The smaller the catchment area is, the greater the extent of cracked ground surface and gully intensity would be. The geomorphologic evolution mechanism of gullied catchments can be explained as a function of geomorphologic indicators of inter-gullied and gullied land characteristics.

关键词： Inter-gullied land erosion Gully erosion Small catchments Gullied land area Loess Plateau

Remote sensing analyses of spatio-temporal changes of the ecological environment in the lower reaches of the Tarim River

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Remote sensing analyses of spatio-temporal changes of the ec...

The First International Conference on Computers and Computing Technologies in Agriculture

作者： YAN ZHENGLONG~(1 2 *) HUANG QIANG~1 TIAN HUIFANG~3 JIN XIAOCHUN~4 1 college of Hydraulic and electrical engineering Xi’an university of Technology 5.South Jinhua Road Xi’an City 710048 PR china 2 Aerial Photogrammetry and Remote Sensing Bureau 3 Jianxi Road Xi’an City 71005. PR china 3 Baoji university of Arts and Sciences 44 Baoguang Road Baoji City 721007 PR china 4 Ordnance engineering college 97 Heping Road Shijiazhuang City 05.003 PR china

＜正＞The ecological environment in the Tarim River Basin is extremely *** ecological problems resulting from local economic development in this region have been a hot topic for the ecological *** is a new trend to use information technologies to monitor the ecological environment dynamically by means of the modern information *** meet the constant requirements of long-term monitoring of a region ecological environment,this paper focuses on the vegetation coverage and land desertification on the basis of the information technologies of remote sensing（RS）,geographic positioning systems（GPS）, geographic information systems（GIS）,and data warehouse,applying the theory of the integrative spatial and attribute data,seamless integration of multi-source *** ecological environment for the lower reaches of the Tarim River is analysed qualitatively and *** investigated database is established and managed through multisource and multi-type massive data,which are collected from three periods of the remote sensing images and background data in 1999,2002 and 2004, *** methods of system classification, remote sensing interpretation,vegetation coverage, conversion models construction,and massive data built database are applied to analyse the data concerned during the dynamically monitoring the ecological environment in the Tarim River Basin. The results show that the vegetation coverage has increased gradually each year after the emergent water transportation project was started,whereas the desertification area has decreased,some sand areas have obviously *** speed of improvement in the east part of the fiver has been higher than in the *** ecological environment in the lower reaches of the Tarim River has tended to improve after the emergent water transportation project in the lower reaches of the Tarim River *** conclusion, the emergent water delivery plays a decisive and significant role in promoti

关键词： database ecological environment remote sensing Tarim River

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“三链相扣”激活农村电子商务

农学学报 2011年第A1期1卷 35-38,46页

作者：高万林王进李桢张港红江新兰蒋宏彪中国农业大学信息与电气工程学院北京100083

结合当前中国农村电子商务的发展现状,从全国农村电子商务的角度分析指出了大规模市场运作的基本条件已经具备,目前需要一种适应当前形势的方案来激活、启动整个农村电子商务市场这一关键问题。就中国农村电子商务的特点和国情提出了农... 详细信息

结合当前中国农村电子商务的发展现状,从全国农村电子商务的角度分析指出了大规模市场运作的基本条件已经具备,目前需要一种适应当前形势的方案来激活、启动整个农村电子商务市场这一关键问题。就中国农村电子商务的特点和国情提出了农产品加工链、储运链、销售链"三链相扣"激活农村电子商务的构建方案,分析了采用"三链相扣"的优点及其可行性,该构建方案具有立竿见影、持续性强等特点,将会激活农村电子商务,大力促进农业经济发展。

关键词： “三链相扣” 农村电子商务加工链储运链销售链

海涂土壤剖面电导率的协同克立格法估值及不同取样数目的比较研究

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土壤学报 2004年第3期41卷 434-443页

作者：李艳史舟王人潮黄明祥浙江大学环境与资源学院农业遥感和信息技术应用研究所杭州310029

应用地统计学的半方差函数和普通克立格法 ,分析了 2 0m× 2 0m的采样间隔上 ,同一剖面0～ 2 0、2 0～ 4 0、4 0～ 6 0、6 0～ 10 0cm四个层次上海涂土壤电导率的空间相关性和空间分布。并在对协同区域化变量进行交互半方差分析的基础... 详细信息

应用地统计学的半方差函数和普通克立格法 ,分析了 2 0m× 2 0m的采样间隔上 ,同一剖面0～ 2 0、2 0～ 4 0、4 0～ 6 0、6 0～ 10 0cm四个层次上海涂土壤电导率的空间相关性和空间分布。并在对协同区域化变量进行交互半方差分析的基础上 ,用协同克立格法 ,将 0～ 2 0cm层上的土壤样品作为辅助变量 ,其他三层上初始及减少后的土壤样品作为主变量 ,来对 2 0～ 4 0、4 0～ 6 0、6 0～ 10 0cm土层上的电导率进行估值。同普通克立格法相比 ,协同克立格法在这三层估值所产生的均方根误差 (RMSE)减少了 9%到 2 5. 1% ,平均标准误差(ASE)减少了 9 4 %到 2 1 9% ,预测值与实测值之间的相关系数协同克立格法比普通克立格法提高了 4 6 %到85.%。数据分析的结果表明 ,利用协同克立格法 ,深层的样品数目在被减少至 6 0 %的情况下仍能满足精度要求 ,没有明显地导致空间信息的丢失。因此 ,协同克立格作为一种更为精确和经济的方法来对样品少的或难以测试的变量进行估值不但可以大大提高估测精度 ,而且可以减少采样数目 ,节省采样的成本。

关键词：海涂土壤电导率协同克立格法取样数目空间便宜估值半方差分析预测精度地统计学

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柠檬醛对黄曲霉质膜物理损伤机制的研究

武汉大学学报（理学版） 2002年第2期48卷 217-222页

作者：罗曼蒋立科邹国林安徽农业大学生物工程系安徽合肥23003 武汉大学生命科学学院湖北武汉430072

通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2）所对应的平均平方振幅、剪切弹性μ和弹性弯曲模量Kc,与理... 详细信息

通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2）所对应的平均平方振幅、剪切弹性μ和弹性弯曲模量Kc,与理论模式光谱相比,研究柠檬醛损伤质膜的物理机制.结果表明,m=1的平动模式主导了光晕波谱,其平均平方振幅呈双指数衰变,该模式几乎与剪切弹性无关,即μ=0;弹性弯曲模量Kc的最小和最大值分别为9.45.10-18J和5.43×10-17J,与柠檬醛的浓度成正相关性.揭示该醛对黄曲霉质膜的损伤,除改变膜的选择通透性和电导率外,同时使质膜平动模式的平均平方振幅呈双指数衰变,弹性弯曲模量Kc增加,从而降低膜伸缩的弹性.

关键词：物理损伤机制柠檬醛黄曲霉质膜反射干涉对比显微镜本征模式分解快速图像分析技术通透性电导率