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Effects of Huangqi (Hex) on Inducing Cell Differentiation and Cell Death in K5.2 and HEL Cells

Acta Biochimica et Biophysica Sinica 2004年第3期36卷 211-217页

作者： Xiao-Dong CHENG1, Chun-Hui HOU1, Xue-Jun ZHANG2, Heng-Yue XIE1, Wei-Ying ZHOU1, Lei YANG2, Shu-Bing ZHANG1, and Ruo-Lan QIAN1 State Key Laboratory of Molecular biology, institute of Biochemistry and Cell biology, Shanghai institutes for Biological sciences, the{5., 3.0 Yueyang Road, Shanghai 20003., China Laboratory of Molecular Cell biology, institute of Biochemistry and Cell biology, Shanghai institutes for Biological sciences, the{5.,3.0 Yueyang Road Shanghai 20003.,China the{5., 3.0 Yueyang Road, Shanghai 20003., China State Key Laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Huangqi (Astragalus membranaceus), a traditional chinese medicine, has been used to ameliorate side effects of cancer chemotherapy in China. However, little is known about its molecular mechanisms. Here we show that induction of K5.2 or HEL cells with 1.5.mg/ml of Huangqi (Hex) (Components extracted from Huangqi) for 3.5.d results in the expression of β-globin gene in both cell lines and leads to terminal differentiation. Moreover, the apoptosis in HEL cells can be induced by increasing concentration of Huangqi (Hex) to 4.5.mg/ml for 3.5.d. Upregulation of Apaf-1, caspase-3.and acetylcholinesterase (AChE) in HEL cells may play a crucial role in the process of apoptosis. The prospect of inducing expression of adult (β) globin gene and apoptosis selectively in cancer cells is obviously attractive from a therapeutic point of view.

关键词： cell death and differentiation Huangqi (Hex) human erythroleukemia cell lines

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Adenovirus-mediated expression of pig α(1,3. galactosyltransferase reconstructs Gal α(1,3. Gal epitope on the surface of human tumor cells

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Cell Research 2001年第2期11卷 116-124页

作者： XING LI, GUO HONG XIA, JIAN FEI, FANG HUANG, LI HE GUO institute of Biochemistry and Cell biology, Shanghai institutes for Biological{3., chinese academy of sciences, Shanghai 20003., China 1institute of Biochemistry and Cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1institute of Biochemistry and Cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1institute of Biochemistry and Cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1institute of Biochemistry and Cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1institute of Biochemistry and Cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Gal α(1, 3. Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by α(1, 3. galactosyltransferase [α(1, 3. GT] enzyme on the cells of lower mammalian animals such as pigs and mice. Human has no gal epitope due to the inactivation of α(1, 3. GT gene but produces a large amount of antibodies (anti-Gal) which recognize Gal α(1, 3. Gal structures specifically. In this study, a replication deficient recombinant adenoviral vector Ad5.GT containing pig α(1, 3. GT cDNA was constructed and characterized. Adenoviral vector-mediated transfer of pig α(1, 3. GT gene into human tumor cells such as malignant melanoma A3.5. stomach cancer SGC-7901, and lung cancer SPC-A-1 was reported for the first time. Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis, although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after Ad5.GT transduction. Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I (UEA I) lectins, Vicia villosa agglutinin (VVA), Arachis hypogaea agglutinin (PNA), and Glycine max agglutinin (SBA) to different degrees. In addition, no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay.

关键词： Adenoviral vector galactosyltransferase Gal α(1 3. Gal gene expression human tumor cell.

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Absolute radiometric calibration of CBERS-02 IRMSS thermal band

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Science China(Technological sciences) 2005年第S2期48卷 72-90页

作者： ZHANG Yong1,2,3. GU Xingfa1,3.4, YU Tao1,3. ZHANG Yuxiang5. CHEN Liangfu1,3. LI Xiaoying1,2,3. LI Xiaowen1,3.& HE Liming6 1. State Key Laboratory of Remote Sensing Science, Jointly Sponsored by the institute of Remote Sensing Applications of chinese academy of sciences and Beijing Normal University, Beijing 100101, China 2. Graduate University of chinese academy of sciences, Beijing 10003., China 3. The Center for National Spaceborne Demonstration, Beijing 100101, China 4. Institut National de la Recherche Agronomique, Climat Sol Environnement, 84914 Avignon, France 5. National Satellite Meteorological Center, Beijing 100081, China 6. State Environmental Protection Administration, Beijing 100029, China chinese Acad Sci Inst Remote Sensing Applicat State Key Lab Remote Sensing Sci Beijing 100101 Peoples R China Beijing Normal Univ State Key Lab Remote Sensing Sci Beijing 100101 Peoples R China chinese Acad Sci Grad Univ Beijing 100039 Peoples R China Ctr Natl Spaceborne Demonstrat Beijing 100101 Peoples R China INRA F-84914 Avignon France Natl Satellite Meteorol Ctr Beijing 100081 Peoples R China State Environm Protect Adm Beijing 100029 Peoples R China

Based on the laboratory calibration before launch of CBERS-02 IRMSS thermal infrared channel, the onboard blackbody calibration, the radiometric cross- calibration against TERRA MODIS corresponding channel and the in-flight field calibration at Lake Qinghai: water surface radiometric calibration test site of China on Aug. 17, 2004 are carried out in this research. When making onboard blackbody calibration of CBERS-02 IRMSS, it is necessary to understand inside structures and light path in IRMSS camera and receive and process calibration blackbody signals at normal and high temperatures. Onboard blackbody calibration results of each detector are very important to relative radiometric calibration of images processing and absolute radiometric calibration of each detector. In-flight field calibration mainly contains field experiments, obtaining synchronous images, spectrum marching and MODTRAN radiative transmi- ssion computation. Radiometric cross-calibration of CBERS-02 IRMSS thermal band against TERRA MODIS selected 6 times synchronous images of two sensors passing through Lake Qinghai and Lake Taihu from August to December, 2004 to compute the cross calibration data. Combining the in-flight field calibration and radiometric cross calibration data, the absolute radiometric calibration coefficients are calculated by the linear regression method. This new calibration model can obviously control the radiometric calibration uncertainties aroused by the single point method used in the in-flight calibration of CBERS-02 IRMSS, this kind of sensors cannot receive the cosmic background radiation. In this research, the radiometric calibration coefficients obtained through the linear regression model are 8.5. (gain, unit: DN/W m?2sr?1μm?1) and 44.92 (offset, unit: DN).

关键词： CBERS-02 IRMSS thermal band in-flight field calibration onboard blackbody calibration cross calibration.

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Down-regulated expression of atypical PKC-binding domain deleted asip isoforms in human hepatocellular carcinomas

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Cell Research 2001年第3期11卷 223-229页

作者： FANG CHANG MINC, YONG HUA XU Laboratory of Molecular and Cellular Oncology, institute of Biochemistry and Cell biology, Shanghai institutes for Biological{3., chinese academy of sciences, Shanghai 20003., China 1Laboratory of Molecular and Cellular Oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. 1Laboratory of Molecular and Cellular Oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China.

Asip is a mammalian homologue of polarity protein Par-3.of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3.Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5.-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,3.0} aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 5..6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.

关键词： Alternative splicing and polyadenylation A sip/Par-3.Bazooka PDZ-motif protein Polarity protein hepatocellular carcinomas.

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The effects of shock wave and quasi-traveling wave in the mechanical impact test

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Science China(Technological sciences) 2010年第9期53卷 2535-2541页

作者： WU Mei1,5.SUN ZhongFeng2,ZHANG LuMei3.CHEN Yong1 & JI LinHong4 1 institute of High Energy Physics,chinese {3. of {3.,Beijing 100049,China 2 School of Mathematical {3.,Peking University,Beijing 100871,China 3.High School of Central University for Nationalities,Beijing 100081,China 4 institute of Mechanical Design,Department of Precision Instruments and Mechanology,Tsinghua University,Beijing 100084,China 5.{3. of Opto-electronics,chinese {3. of {3.,Beijing 100190,China 1. institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China5. Academy of Opto-electronics Chinese Academy of Sciences Beijing 100190 China2. School of Mathematical Sciences Peking University Beijing 100871 China3. High School of Central University for Nationalities Beijing 100081 China4. Institute of Mechanical Design Department of Precision Instruments and Mechanology Tsinghua University Beijing 100084 China

It is well-known that the numerical value is always larger than the measured value,amounting to many times,if we calculate the stress of the specimen in the impulse test using the NASTRAN and ANSYS (N-A) *** believe that the impact induces shock wave or quasi-traveling wave in the specimen,which can qualitatively explain the discrepancy of the two *** order to verify it,the Lax-Friedrichs (L-F) scheme is taken to simulate the transmission of shock wave and quasi-traveling wave in *** results show that the action area of the stress wave is small and the action time is very short,so the resulting stress and actual work are not *** addition,the distribution of the impact values obtained by the numerical simulation is in accordance with the trend of the measured impact values.

关键词： aerospace environment test shock wave quasi-traveling wave

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Toxic Plant Resources in Panxi Area

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Wuhan University Journal of Natural sciences 2007年第4期12卷 762-768页

作者： XU Yun SU Chunjiang ZHENG Yuanchang institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 Sichuan China

Panxi Area is abundant in plant resources, among which toxic plants are of great value in terms of exploitation. This paper is an initiative study （via field as well as literature investigation） of the categories, distributions, and reserves of toxic plant resources in Panxi Area. The study reveals that there are 5. families （210 species） of toxic plants evenly distributed in Panxi Area, of which more than 40 species grow in all counties in the area, and more than 14 species total a reserve more than 1.0×10^5.kg. These toxic plants are of great applications to medicine, gardening, biopesticide industry, environmental engineering, and oil manufacturing. Rhododendron molle, Anisodus acutangulus, Arisaema erubesocens, Stellera chamaejasme, Rhytolacca acinosa, Rheum officinale, and Azadiralta indica etc are the typical toxic plants with great value of exploitation in Panxi Area.

关键词： toxic plant resources exploitation categories distributions reserves Panxi Area

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泥石流梁式格栅坝拦砂性能试验研究

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水土保持学报 2001年第1期15卷 113-115页

作者：游勇中国科学院水利部成都山地灾害与环境研究所!成都610041

通过特殊泥石流试验装置 ,对泥石流梁式格栅坝拦砂性能进行研究 ,初步得到 :梁式格栅坝有全部闭塞、临时闭塞、半闭塞、未闭塞 4种闭塞类型。当梁间距 b与泥石流体中最大颗粒粒径 dm ax之比 b/ dmax≤ 1.0时 ,格栅坝基本闭塞 ;b/ dmax≥... 详细信息

通过特殊泥石流试验装置 ,对泥石流梁式格栅坝拦砂性能进行研究 ,初步得到 :梁式格栅坝有全部闭塞、临时闭塞、半闭塞、未闭塞 4种闭塞类型。当梁间距 b与泥石流体中最大颗粒粒径 dm ax之比 b/ dmax≤ 1.0时 ,格栅坝基本闭塞 ;b/ dmax≥ 1.5. ,格栅坝未闭塞 ;1.0 5. ,格栅坝半闭塞或临时闭塞。试验结果表明 ,梁式格栅坝闭塞与沟道纵坡有较大关系 ,结构尺寸相同的格栅坝在通过相同泥石流时 ,在不同沟道纵坡条件下有不同闭塞效果。

关键词：泥石流梁式格栅坝模型试验

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Cloning and characterization of vernalization-related gene (ver203.) cDNA 3.end

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chinese Science Bulletin 1999年第14期44卷 1289-1294页

作者： YONG Weidong, CHONG Kang , LIANG Tiebing, XU Zhihong , TAN Kehui and ZHU Zhiqing Research Center for Molecular and Developmental biology, institute of Botany, chinese academy of sciences , Beijing 100093. China Shanghai institute of Plant Physiology , chinese academy of sciences , Shanghai 20003., China Corresponding authors Research Center for Molecular and Developmental biology Institute of Botany Chinese Academy of Sciences Beijing China Shanghai institute of Plant Physiology Chinese Academy of Sciences Shanghai China

Based on the cDNA fragment sequence of vernalization-related gene verc203.cloned by differential screening in our lab, the 5.primer has been designed. The cDNA 3.end of ver203.gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the gene has homology with Hordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate.

关键词： wheat vernalization-related gene cDNA 3. end RACE.

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REACTIONS OF PYRITE IN CATALYTICAL OXIDATION ACID LEACHING SYSTEM

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中国有色金属学会会刊：英文版 1995年第2期5卷 43-48页

作者： Xia, Guangxiang Tu, Taozhi Yang, Hanlin Shi, Wei(institute of Chemical Metallurgy, chinese academy of sciences, Beijing 100080) chinese Acad of sciences Beijing China

ＲＥＡＣＴＩＯＮＳＯＦＰＹＲＩＴＥＩＮＣＡＴＡＬＹＴＩＣＡＬＯＸＩＤＡＴＩＯＮＡＣＩＤＬＥＡＣＨＩＮＧＳＹＳＴＥＭ￥Ｘｉａ，Ｇｕａｎｇｘｉａｎｇ；Ｔｕ，Ｔａｏｚｈｉ；Ｙａｎｇ，Ｈａｎｌｉｎ；Ｓｈｉ，Ｗｅｉ（Ｉｎｓｔｉ... 详细信息

ＲＥＡＣＴＩＯＮＳＯＦＰＹＲＩＴＥＩＮＣＡＴＡＬＹＴＩＣＡＬＯＸＩＤＡＴＩＯＮＡＣＩＤＬＥＡＣＨＩＮＧＳＹＳＴＥＭ￥Ｘｉａ，Ｇｕａｎｇｘｉａｎｇ；Ｔｕ，Ｔａｏｚｈｉ；Ｙａｎｇ，Ｈａｎｌｉｎ；Ｓｈｉ，Ｗｅｉ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｅｍｉｃａｌＭｅｔ...

关键词： catalytic oxidation acidic leaching pyrite oxidation pretreatment

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Simulation of Isotopic Angular Effects under Ion Bombardment of a^(92)Mo-^(100)Mo Target

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Journal of Materials Science & Technology 1997年第1期13卷 65-68页

作者： Liping ZHENG (Shanghai institute of Nuclear Research, chinese academy of sciences, Shanghai 201800, China)Risheng LI and Shuyou LI(Lab. of Atomic Imaging of Solids, institute of Metal Research, chinese academy of sciences,Shenyang 110015. China) Shanghai institute of Nuclear Research Chinese Academy of Sciences Shanghai 201800 China Lab. of Atomic Imaging of Solids Institute of Metal Research Chinese Academy of Sciences Shenyang 110015 China

Under 5.keV Ar ion bombardment of a 92Mo-100Mo target, we have investigated isotopic angular effects by means of the static and the dynamic Monte Carlo programs. Our calculated results are in quantitative agreement with the measured and other calculated results. The conclusion consistences among theories. simulations and measurements are also discussed.

关键词： Mo Target Simulation of Isotopic Angular Effects under Ion Bombardment of a Target

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