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Dynamics of morphological changes for mitochondrial fission and fusion

Science China(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3.& LONG Mian 1,2 1 Key Laboratory of Microgravity (National Microgravity Laboratory), beijing 100190, China 2 Center for Biomechanics and Bioengineering, Institute of Mechanics, chinese academy of sciences, beijing 100190, China 3.State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, chinese academy of sciences, beijing 100101, China 4 Institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, beijing 100044, China 1. Key Laboratory of Microgravity (National Microgravity Laboratory) Beijing 100190 China2. Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China4. Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing 100044 China3. State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

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Challenges and innovations in green process intensification

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Science China Chemistry 2010年第7期53卷 1470-1475页

作者： LIU HuiZhou,LIANG XiangFeng,YANG LiangRong & CHEN JiaYong Institute of Process Engineering,chinese academy of sciences,beijing 100190,China 1. Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China

Process intensification is one of the most significant trends in current chemical engineering and process *** recent years,the desire to become more "green" in processing has always been tied to the requirement of being *** review discusses the challenges of process engineering and summarizes the key role of fundamentals for green process intensification.

关键词： green chemistry process intensification

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Experimental Research on Antiscale and Scale Removal by Ultrasonic Cavitation

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Journal of Thermal Science 2009年第1期18卷 65-73页

作者： LI Hong-xia,HUAI Xiu-lan,CAI Jun and LIANG Shi-qiangInstitute of Engineering Thermophysics,chinese academy of sciences,beijing,100190,China Professor Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing China

Aiming at the scale problem in heat-transfer equipments, experimental investigation on antiscale and scale removalby ultrasonic cavitation is performed. By means of microscopic magnifying photography system, thesedimentary phenomenon can be observed. The experimental research reveals the influencing rule of acoustic intensity,cavitational distance, liquid temperature and solution concentration. The experimental results indicate thatliquid temperature has different effects on antiscale and scale removal. Different experimental liquids are used forantiscale and scale removal experiments. The results show that every liquid has a respective Cavitational activetemperature. When ultrasonic is used for antiscale, the smaller acoustic intensity is, the better effect is. But, whenultrasonic is used for scale removal, acoustic intensity has a reverse influence. In addition, biggish solution concentrationis propitious to antiscale for long-time running. Distance of test sample to ultrasonic transducer alsohas certain influence on antiscale and scale removal. The smaller the distance to ultrasonic transducer is, the bettereffects antiscale and scale removal have.

关键词： ultrasonic cavitation acoustic cavitation antiscale scale removal acoustic intensity

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Teleconnection between the Indian summer monsoon onset and the Meiyu over the Yangtze River Valley

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Science China Earth sciences 2008年第7期51卷 1021-1035页

作者： LIU YunYun1,2,3.& DING YiHui2 1 chinese academy of Meteorological sciences, {3. 100081, China 2 National Climate Center, {3. 100081, China 3.Graduate University of chinese academy of sciences, {3. 100049, China 1. chinese academy of Meteorological sciences Beijing 100081 China 2. National Climate Center Beijing 100081 China 3. Graduate University of chinese academy of sciences Beijing 100049 China

Based on the Indian and chinese precipitation data and the NCEP-NCAR reanalysis circulation data, the relationship between the Indian summer monsoon (ISM) onset and the Meiyu over the Yangtze River Valley has been discussed by the methods of correlation analysis and composite analysis. The results show that the date of ISM onset over Kerala in the southwestern coast of the Indian Peninsula is about two weeks earlier than the beginning of the Meiyu over the Yangtze River Valley. After the outbreak of ISM, the teleconnection mode sets up from the western coast of India via the Bay of Bengal (BOB) to the Yangtze River Valley and southern Japan. It is different both in time and space from the telecon- nection mode which is from the northwest of India via the Tibetan Plateau to northern China. The for- mer mode is defined as the "south" teleconnection of the Asian summer monsoon, forming in the pe- riod of ISM onset; while the latter mode is called the "north" teleconnection, mainly occurring in the Asian monsoon culminant period. During the process of the "south" teleconnection’s formation, the Asian monsoon circulation has experienced a series of important changes: ISM onset, the northward movement of the south Asia high (SAH), the onset vortex occurrence, the eastward extension of the stronger tropical westerly belt, and the northeastward jump of the western Pacific subtropical high (WPSH), etc. Consequently, since ISM sets up over Kerala, the whole Asian continent is covered by the upper SAH after about two weeks, while in the mid- and lower troposphere, a strong wind belt forms from the Arabian Sea via the southern India, BOB and the South China Sea (SCS), then along the western flank of WPSH, to the Yangtze River Valley and southern Japan. With the northward moving of the subtropical jet streams, the upper westerly jet stream and the low level jet have been coupled ver- tically over east Asia, while the Yangtze River Valley happens to locate in the ascending motion

关键词： Indian summer monsoon (ISM) the Meiyu over the Yangtze River Valley teleconnection mode

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Chronology of Holocene lacustrine sediments in Co Ngoin, central Tibetan Plateau

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Science China Earth sciences 2006年第9期49卷 991-1001页

作者： WU Yanhong1, WANG Sumin1& HOU Xinhua1,3.1. Key Laboratory of Lake Sedimentation and Environment, Nanjing Institute of Geography and Limnology, chinese academy of sciences, Nanjing 210008, China 2. Institute of Tibetan Plateau Research, chinese academy of sciences, beijing 10085, China 3. Graduate University of chinese academy of sciences, beijing 10003., China 1. Key Laboratory of Lake Sedimentation and Environment Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China 2. Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 10085 China 3. Graduate University of chinese academy of sciences Beijing 100039 China

Chronology is the basis for using lacustrine sediments to reconstruct the history of en-vironmental change. Radioactive-nuclides such as 14C, 210Pb and 13.Cs dating are mainly used to establish the chronology for recent several ten thousand years. Because of being mixed with “dead carbon”, the measured 14C age is always older than the actual age which is recognized as “reservoir effect”. Cs is a kind of active metal element, and easy to migrate vertically in the sediment that leads to the error of the time marker. 210Pb dating should make sure to select CIC model or CRS model. On the Tibetan Plateau, most of the lakes are alkaline closed lake where Cs is more moveable in the sediment and the reservoir effect caused by “dead carbon” on 14C dating is stronger. Based on the analysis on results of 14C, 210Pb and 13.Cs of the lacustrine sediments from Co Ngoin, central Tibetan Plateau, we use the simple regression method to re-calculate the 14C calendar ages, and establish the time sequence for cores CE-1 and CE-2 with result of 210Pb CRS model for the upper 3. cm and re-calculated 14C calendar age for sediments bellow 3. cm. Since 1400 cal. a BP, sedimentation inter-rupted for more than 1000 years. According to this time sequence, variations of environmental proxies confidently reflect the major climatic events in Holocene.

关键词： 14C, 210Pb and 13.Cs, chronology, lacustrine sediments, climatic event, Co Ngoin, central Tibetan Plateau.

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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3. Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese academy of sciences, beijing 100080, China 2Center of Mass Spectrometry, Institute of Chemistry, chinese academy of sciences, beijing 100080, China 3.raduate School of the chinese academy of sciences, beijing 10003., China Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China Graduate School of the chinese academy of sciencesbeijing 10003. China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 3.9 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

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NONLINEAR FLUID DAMPING IN STRUCTURE-WAKE OSCILLATORS IN MODELING VORTEX-INDUCED VIBRATIONS

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Journal of Hydrodynamics 2009年第1期21卷 1-11页

作者： LIN Li-ming LING Guo-can WU Ying-xiang ZENG Xiao-hui Division of Engineering sciences Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China The State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China

A Nonlinear Fluid Damping （NFD） in the form of the square-velocity is applied in the response analysis of Vortex-Induced Vibrations （VIV）. Its nonlinear hydrodynamic effects on the coupled wake and structure oscillators are investigated. A comparison between the coupled systems with the linear and nonlinear fluid dampings and experiments shows that the NFD model can well describe response characteristics, such as the amplification of body displacement at lock-in and frequency lock-in, both at high and low mass ratios. Particularly, the predicted peak amplitude of the body in the Griffin plot is in good agreement with experimental data and empirical equation, indicating the significant effect of the NFD on the structure motion.

关键词： Vortex-Induced Vibration (VIV) Nonlinear Fluid Damping (NFD) wake oscillator

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CONVERGENCE OF VORTEX METHODS FOR 3.D EULER EQUATIONS

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Journal of Computational Mathematics 2000年第3期18卷 239-250页

作者： Jia-fu Lin (Institute of System Science, academy of Mathematics and System sciences, chinese academy of sciences, {3., 100080, China School of Mathematical sciences, Peking University, {3. 100871, China) 中科院系统科学所北京 100080

Presents a study on the convergence of vortex methods for the three-dimensional Euler equations in bounded domains. Related studies on the convergence of vortex methods; Formulation of vortex methods; Convergence for ... 详细信息

关键词： Euler equations vortex methods convergence initial-boundary value problem

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Analysis of tandem repeats in the genome of chinese shrimp Fenneropenaeus chinensis

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chinese Science Bulletin 2005年第14期50卷 1462-1469页

作者： KONG Jie1 & GAO Huan2,3.1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, chinese academy of Fishery sciences, Qingdao 266071, China 2. Institute of Oceanology, {3., Qingdao 266071, China 3. The Graduate School of the {3., beijing 10003., China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheris Research Institute Chinese Academy of Fishery Sciences Qingdao China Institute of Oceanology Chinese Academy of Sciences Qingdao China The Graduate School of the chinese academy of sciences Beijing China

Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2159 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 20.6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2% of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42%. In decreasing order, the 20 most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (274), AAT (92), A (56), AAG (28), ATC (27), ATAG (27), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them.

关键词：虾遗传特性遗传基因 DNA

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IMPLICITIZATION USING UNIVARIATE RESULTANTS

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Journal of Systems Science & Complexity 2010年第4期23卷 804-814页

作者： Liyong SHEN Chunming YUAN School of Mathematical sciences Graduate University Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Mathematics Mechanization Institute of Systems Science Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 China

Among several implicitization methods, the method based on resultant computation is a simple and direct one, but it often brings extraneous factors which are difficult to remove. This paper studies a class of rational space curves and rational surfaces by implicitization with univaxiate resultant computations. This method is more efficient than the other algorithms in finding implicit equations for this class of rational curves and surfaces.

关键词： Implicitization rational space curves rational surfaces resultants.

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