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生物工程学报 2003年第2期19卷 131-135页

作者：刘昱辉贾士荣中国农业科学院生物技术研究所北京100081

综述了维管束特异表达启动子及其顺式调控元件和基元序列调控基因表达的研究进展 ,它们在抗细菌、真菌性维管束病害的作物基因工程中具有广阔的应用前景。

关键词：维管束特异启动子顺式调控元件基元序列基因表达调控

The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice

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chinese Science Bulletin 2001年第13期46卷 1080-1084页

作者： JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. institute of Agrobiological Genetics and Physiology, Jiangsu academy of agricultural sciences, Nangjing 210014, china.2. institute of Botany, the chinese academy of sciences, beijing 100093, china.3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nangjing China institute of Botany The Chinese Academy of Sciences Beijing China Botany Department Washington State University Pullman USA

With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC

关键词： transgenic rice photosynthetic characteristics photoin-hibition of photosynthesis chlorophyll fluorescence phosphoenopy-ruvate carboxylase (PEPC).

Forty Years' Development of Hybrid Rice: china.s Experience

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Rice Science 2004年第Z1期 225-230页

作者： CHENG Shi-hua , CAO Li-yong , YANG Shi-hua , ZHAI Hu-qu (china.National Rice research institute, Hangzhou 310006, china.chinese academy of agricultural sciences, beijing 100081, china.

china.is the first country where heterosis of hybrid rice was successfully exploited commercially on a large scale in the world Hybrid rice has been developed for 40 years since Prof. Yuan initiated it in china. It had been planted about 330 million hectares with an increase of nearly 400 million tons of rice during 1976-2002. china.s experience on hybrid rice could be attributed to utilization of various cytoplasmic male sterility (CMS) resources, high outcrossing rate CMS lines and stable environmentally induced genetic male sterile (EGMS) lines, improvement of diseases resistance and grain quality, and combination of ideal plant type with heterosis in hybrid rice breeding program. Innovative breeding techniques, e.g. improvement in root system, molecular marker-assisted selection and wild hybridization should be considered in further development of hybrid rice in china.

关键词： hybrid rice breeding progress prospect

The Supply and Demand of agricultural Products in china.Based on Balanced Diet

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Asian agricultural research 2012年第8期4卷 5-10页

作者： ZHOU Zhen-ya,ZHANG Qing,GAO Ming-jie,LI Jian-ping institute of agricultural Resources and Regional Planning,chinese academy of agricultural sciences,beijing 100081,china

Promoting agricultural production and ensuring the supply of agricultural products has always been the main task of agricultural development in china.but the agricultural production in china.has not yet been combined with the chinese residents' dietary needs to formulate reasonable development goals,with a certain blindness in production. According to the dietary standards in The Dietary Guidelines for chinese Residents developed by the chinese Nutrition Society,we calculate the demand of various types of agricultural products in china.under the conditions of balanced diet. In comparison with the output of various types of agricultural products in china.at present,we find that the output of most of agricultural products in china.has exceeded the reasonable demand of the chinese residents under the conditions of balanced diet. Therefore,adjusting the agricultural production structure in china.and advocating balanced diet has become an important way to solve the problem of balance between supply and demand of agricultural products.

关键词： Balanced diet agricultural products Supply and dem

DEVELOPMENT OF TRANSGENIC COTTON RESISTANT TO FUSARIUM AND VERTICILLIUM WILT

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DEVELOPMENT OF TRANSGENIC COTTON RESISTANT TO FUSARIUM AND V...

Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer

The ⅩⅤth International Plant Protection Congress

作者： WANG Zhi-xing CHENG Hong-mei JIA Shi-rong Biotechnology research institute chinese academy of agricultural sciences beijing 100081 china

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Science china.Life sciences) 2010年第4期53卷 517-523页

作者： PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 institute of Animal sciences, chinese academy of agricultural sciences, beijing 100193, china.2 beijing institute of Biotechnology, beijing 100071, china.3 china.agricultural University, beijing 100193, china.4 Yutian Breeding Swine Farms, Yutian 064104, china 1. institute of Animal sciences Chinese Academy of Agricultural Sciences Beijing 100193 China2. Beijing Institute of Biotechnology Beijing 100071 China3. China Agricultural University Beijing 100193 China4. Yutian Breeding Swine Farms Yutian 064104 China

Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in china. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.

关键词： sFat-1 nuclear transfer ovulation status transgenic pig

A KNOWLEDGE MODEL-AND GROWTH MODEL-BASED MANAGEMENT SYSTEM FOR CORN

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A KNOWLEDGE MODEL-AND GROWTH MODEL-BASED MANAGEMENT SYSTEM F...

Distribution of Grain Hardness in chinese Wheats and Genetic Analysis

The 3rd International Symposium on Intelligent Information Technology in Agriculture(ISIITA)

作者： Dingchun Yan Yeping Zhu Shijuan Li institute of agricultural Information chinese academy of agricultural sciences beijing 100081 china

＜正＞By analyzing and extracting of the results of research on corn（Zeamays L.）cultural theories and technologies,a growth model（GM）and a knowledge model（KM）for corn management have been developed using the system’s analysis principle and mathematical modeling technique and which are driven by dynamic relationships and quantitative algorithms of corn growth and management indices to variety traits, eco-environments and production *** on the platforms of Microsoft Visual C++.NET,a comprehensive and digital knowledge model- and growth model-based management system for corn （KMGMSC）has been established using soft component Case studies on the KMGMSC with the data sets of different climatic years,soil types and cultivars at different coo-sites,and comparative field experiments indicated a good performance of the model system in decision-making and guidance for corn management.

关键词： corn knowledge model growth model soft component

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agricultural sciences in china 2002年第11期1卷 1190-1198页

作者： ZHOU Yan-hua, HE Zhong-hu, YAN Jun, ZHANG Yan, WANG De-sen and ZHOU Gui-ying(institute of Crop Breeding and Cultivation, chinese academy of agricultural sciences / National Wheat Improvement Center,beijing 100081 ,P. R. china.CIMMYT china.Office, C/OCAAS, beijing 100081 ,P. R. china. institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences/National Wheat Improvement CenterBeijing 100081 P.R.China institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences/National Wheat Improvement CenterBeijing 100081 P.R.China CIMMYT china.Office C/O CAAS Beijing 100081 P.R.China

A hundred winter wheat and 41 spring wheat cultivars and advanced lines were used to investigate the distribution of grain hardness in chinese wheats and correlations between grain hardness and other kernel traits. P1, P2, F1 , F2 and F3 from three crosses, i. e. , Liken2/Yumai2, 85Zhong33/Wenmai6 and 85Zhong33/95Zhong459 were sown to study the genetics of grain hardness. Significant correlation was observed between hardness measured by Single Kernel Characteristic System 4100 (SKCS 4100) and Near Infrared (NIR) Spectroscopy, r ranging from 0.85 to 0.94. chinese wheat is a mixed population in terms of hardness, ranging from very soft to very hard. For autumn-sown wheat, on average, grain hardness decreases from north to south and spring-sown wheat is dominant with hard type. Hardness is negatively associated with flour color, and its associations with flour yield and ash content differ in winter and spring wheats. Grain hardness is controlled by a major gene and several minor genes with additive effect mostly, but dominant effect is also observed, with heritability of 0.78.

关键词： T. aestivum,Common wheat Grain hardness Processing quality Genetic analysis

EXPRESSION IN Yersinia enterocolitica OF K99 ANTIGEN GENE OF Escherichia coli

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Science in china.Ser.B 1988年第10期31卷 1213-1221页

作者：孙午良范云六 Laboratory of Molecular Biology Biotechnology research Center chinese academy of agricultural sciences beijing

The expression of the determinant for the K99 antigen of Escherichia coli in Yersinia enterocolitica avirulent transconjugants D29(pFS239) and L15(pFS239) was confirmed by means of SDS-polyacrylamide gel electrophoresis, double immunodiffusion and Western blotting. The conditions for the expression of K99 antigen genes in Y. enterocolitica and in E. coli were compared. The K99 antigen protein expressed in Y. enterocolitica in the form of pili on the surface of the cells was discussed. This article offers the first description on the expression in Y. enterocolitica of gene from organism other than Yersinia spp.

关键词： E. coli K99 antigen gene gene expression Y. enterocolitica.

CLONING IN Yersinia enterocolitica OF K99 ANTIGEN GENE FROM Escherichia coli

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Science in china.Ser.B 1988年第8期31卷 944-952页

作者：孙午良范云六 Laboratory of Molecular Biology Biotechnology research Center chinese academy of agricultural sciences beijing

A recombinant plasmid pFS239 containing the geoe coding for K99 antigen of Escherichia coli and wide-host-range plasmid pKT230 has been cloned in E. coli C600. pFS239 has been transferred to Yersinia enterocolitica strains D29, L15 and L15 (pYV15) through triparental mating. In Y. enterocolitica transconjugants the expression of VW antigens and calcium dependence which represent the propertics associated with the virulence plastmid of Y. enterocolitica remains unchanged.

关键词： Yersinia enterocolitlca K99 antigen recombinant plasmid triparental mating.