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Acta Pharmacologica sinica 2007年第10期28卷 1597-1602页

作者： Qing-chuan LIAO~2,Zhou-sheng XIAO~(4,5),Yan-fang QIN~3,Hong-hao ZHOU~4~2 Affiliated children's hospital,nanjing Medical University,nanjing 210008,china,~3 department of Pathology,school of Medicine,JinanUniversity,Guangzhou 510632,china ~4 Institute of Clinical Pharmacology,Central south University,Changsha 410078,china ated childrenos hospital Nanjing Medical University Nanjing 210008 China. xiaozs64@***.

Aim:To test the hypothesis that genistein stimulates the osteoblastic differentia-tion through the p38 mitogen activated protein kinase (MAPK)-core-binding fac-tor 1 (Cbfa1) ***:The activation of p38 MAPK was detected byWestern *** phosphatase (ALP) activity and calcium depositionwere assessed for osteoblastic differentiation of bone marrow-derived mesenchy-mal stem cell (BMsC) *** expression of Cbfa1 was analyzed at both themRNA and protein *** activity of Cbfa1 was detected by electrophoreticmobility shift *** sialoprotein (BsP),ALP,osteocalcin (OC),andosteopontin (OPN) gene transcription were also evaluated by either RT-PCR orNorthern ***:Genistein (0.01-1 μmol/L) dose dependently led tothe rapid and sustained activation of the p38 MAPK pathway in mouse *** with genistein (1 μmol/L) resulted in increased ALP activityand calcium deposition of BMsC cultures as a function of *** alsoenhanced Cbfa1 DNA binding activity and promoted the expressions of Cbfa1itself as well as several Cbfa1-regulated genes,including ALP,BsP,OC,and *** treatment with p38 MAPK inhibitor (sB203580) diminished thegenistein-induced osteoblastic maturation and p38 MAPK-Cbfa1 activation inmouse BMsC ***:These results indicated that genistein couldstimulate the osteoblastic differentiation of BMsC cultures through the p38MAPK-Cbfa1 pathway.

关键词： bone marrow-derived mesenchymal stem cells core-binding factor 1 osteoblastic differentiation genistein mitogen-activated protein kinase

Application of laparoscopic cholecystectomy in patients with cirrhotic portal hypertension

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Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 270-274页

作者： Wu Ji, Ling-Tang Li, Xun-Ru Chen and Jie-shou Li nanjing, chinaResearch Institute of General surgery, nanjing Ge- neral hospital of nanjing PLA Command, nanjing 210002, china and department of Hepatobiliary surgery, Kunming General hospital of Chengdu PLA Command, Kunming 650032, china Research Institute of General surgery of PLA Nanjing General Hospital of Nanjing PLA Command Nanjing 210002 China.

BACKGROUND: Laparoscopic cholecystectomy (LC) has been widely adopted in treating benign gallbladder disea- ses. Cirrhosis and cirrhotic portal hypertension (CPH) are contraindicated for LC in its early period. In recent years, several studies have reported liberal use of LC in patients with cirrhosis. But its benefits and successful use in patients with CPH are less documented. This study was designed to evaluate the feasibility, safety and technical characteristics of LC in CPH patients. METHODs: In 38 patients with symptomatic gallbladder disease and CPH, 19 belonged to Child A class, 15 Child B class and 4 Child C class. Perioperative data of these pa- tients were collected and analyzed. REsULTs: LC was successfully performed in 36 patients, and 2 patients (5.3%) were converted to open cholecystec- tomy (OC) for difficulty in management of bleeding under laparoscopy and dense adhesion of Calot' s triangle. The sur- gical time was 62.6±15.2 minutes. The estimated amount of intraoperative hemorrhage was 75.5±15.5 ml. No blood transfusion was necessary. The time to resume diet was 18.3 ±6.5 hours. seven postoperative complications in 5 patients (13.2%) included port-site infection (1 patient), respiratory infection (2), upper digestive tract bleeding (1), slight hepatic encephalopathy (1) and increased ascites (2). All patients were cured and discharged from the hospital within 5.6±2.4 days after LC. CONCLUsIONs: Despite LC is difficult for CPH patients, it is feasible and relatively safe. To make LC successful in patients with CPH, it is necessary for surgeons to acquaint with the technical characteristics of LC and emphasize me- ticulous perioperative management.

关键词： cirrhosis portal hypertension laparoscopic cholecystectomy

A New Oxoaporphine Alkaloid from Alphonsea Mollis Dun.

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Chinese Chemical Letters 1999年第8期10卷 675-676页

作者： Ning XIE Zhi Hua CHENG Xiao Gan YU (department of Phytochemistry china Pharmaceutical University, nanjing 210009)(Zhongkun Institute of Pharmaceutics, china Pharmaceutical University, nanjing 210009)(Jiangxi provincial children’s hospital, Nanchang 3 china Pharmaceut Univ Dept Phytochem Nanjing 210009 Peoples R China china Pharmaceut Univ Zhongkun Inst Pharmaceut Nanjing 210009 Peoples R China Jiangxi prov childrens Hosp Nanchang 330006 Peoples R China

A new oxoaporphine alkaloid, 1,2-methylene-dioxy-8-hydroxy-5- oxoaporphine, was isolated from the stem barks of Alphonsea mollis . Its structure was established on the basis of spectral analysis and chemical correlation.

关键词： Alphonsea mollis oxoaporphine 1.2-methylenedioxy-8-hydroxy-5-methoxy oxoaporphine

Role of laparoscopic subtotal cholecystectomy in the treatment of complicated cholecystitis

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 584-589页

作者： Wu Ji, Ling-Tang Li and Jie-shou Li Research Institute of General surgery, nanjing General hospital of nanjing PLA Command Area, nanjing 210002, china Research Institute of General surgery Nanjing General Hospital of Nanjing PLA Command Area Nanjing 210002 China.

BACKGROUND: Laparoscopic cholecystectomy (LC) has become the 'gold standard' in treating benign gallbladder diseases. Increasing laparoscopic experience and techniques have made laparoscopic subtotal cholecystectomy (LsC) a feasible option in more complex procedures. In recent years, few studies with a few cases of LsC have reported good results in patients with various types of cholecystitis. This study was designed to evaluate the feasibility, indications, characteristics and benefits of LsC in patients with complicated cholecystitis. METHODs: Altogether, 3485 patients were scheduled to receive LC during the past 4 years at our institute. Among them, 168 patients with various complicated forms of cholecystitis were treated by LsC. Meanwhile, the other 3317 patients who received standard LC were enrolled as the control group. Perioperative data from the two groups were collected and retrospectively analyzed. REsULTs: In the LsC group, 135 patients suffered from acute calculic cholecystitis, 18 from chronic calculic cholecystitis with cirrhotic portal hypertention, and 15 from chronic calculic atrophy cholecystitis with severe fibrosis. These patients constituted 4.8% of the total patients who underwent LC (168/3485) in the same period at our institute. In 122 patients, the cystic duct and artery were clipped before division. In another 46 patients, the gallbladder was initially incised at Hartmann's pouch. Five patients (3.0%) were converted to open subtotal cholecystectomy. The median operation time for LsC was 65.5±15.2 minutes, estimated operative blood loss was 71.5±15.5 ml, and the time to resume diet was 20.4±6.3 hours. Thirteen patients (7.7%) had local complications. The mean postoperative hospital stay was 4.2±2.6 days. In the LC group, 2887 had chronic calculic cholecystitis, 312had acute calculic cholecystitis, 47 had chronic calculic atrophy cholecystitis, and 71 had polypus. seventeen patients (0.5%) were converted to open cholecystectomy. The median o

关键词： laparoscopic cholecystectomy cholecystitis cirrhotic portal hypertension complication bile duct injury

Basic study on architectural planning of psychiatric hospital

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science china(Technological sciences) 2010年第7期53卷 1755-1767页

作者： ZHOU Ying1 & sUN YaoNan2 1 southeast University, nanjing 210096, china 2 nanjing University of science & Technology, nanjing 210094, china 1. southeast University Nanjing 210096 China2. Nanjing University of Science & Technology Nanjing 210094 China

This paper considers the main problems of the current mental health facilities in china from the standpoint of architects on the background that mental disorder has become a serious threat to human health. In order to effectively learn from the developed countries, this paper reviews the brief history of the world's mental health and the main phases of the evolution of psychiatric hospitals, analyzes the actual situation of mental health facilities abroad based on a large amount of data, compares the roles of public and private psychiatric hospitals from the viewpoint of government supervision, and investigates the functions and positions of psychiatric hospitals and the psychiatry divisions of general hospitals in the medical system. At last, this paper discusses the necessity of the systemization of mental health facilities, the role of psychiatric hospitals in this facility system and the developing approaches of psychiatric hospitals in china.

关键词： medical facility for mental disorder facility system psychiatric hospital principles

Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2011年第3期10卷 289-294页

作者： Research Center of Clinical Medicine,Affiliated hospital of Nantong University,Nantong 226001,china (Yao M,Yao DF,Qiu LW,Wu W,sai WL,Yang JL and Zhang HJ) department of Oncology,Yancheng First People’s hospital,Yancheng 224001,china (Bian YZ) department of Oncology,second Affiliated hospital,nanjing Medical University,nanjing 210011,china (Zhang CG) [a] Research Center of Clinical Medicine Affiliated Hospital of Nantong University Nantong 226001 China [b] department of Oncology Yancheng First People's Hospital Yancheng 224001 China [c] department of Oncology Second Affiliated Hospital Nanjing Medical University Nanjing 210011 China

BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor *** prognosis is poor and early diagnosis is of utmost *** study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for ***:A hepatoma model was induced in male sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) *** RNA was purified and transcribed into cDNA by reverse *** of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by ***-3 was analyzed by immunohistochemistry,Western blotting or ***:Positive GPC-3 expression showed as brown granule-like staining localized in the *** examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT *** incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,*** was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,Pserum GPC-3 (r= 0.994,PsION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC.

关键词： hepatocellular carcinoma glypican-3 immunohistochemistry nested RT-PCR

Influence of CYP2D610B genotype on pharmacokinetics of propafenone enantiomers in Chinese subjects

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Acta Pharmacologica sinica 2003年第12期 103-106页

作者： CHEN Bing CAI Wei-Min department of Clinical Pharmacology, Jinling hospital, nanjing 210002, china

AIM: To study the relationship between genotype of CYP2D6* 10B and pharmacokinetics of propafenone enantiomers. METHODs: Genotype of 17 healthy Chinese HAN subjects was determined by an allele specific amplification method. The blood samples (0-15 h) of the subjects were taken after oral administration of a single dose (400 mg) of propafenone hydrochloride. Concentrations of propafenone enantiomers in plasma were measured by a reverse-phase HPLC with precolumn derivatization. REsULTs: seventeen subjects characterized for CYP2D6* 10B genotype included (*1/*1) (n=4), (*1/*10) (n=5) and (*10/*10) (n=8). The metabolic ratios (lg MR) of the three genotypes were-2.68±0.23, -2.2±0.7, and -1.1±0.5, respectively. The AUC of the three groups were (1534±334), (1891±793), (3171±1075) μg·h·L-1for s-enantiomer and (1136±345), (1467±817), (2277±745) μg·h·L-1for R-enantiomer, respectively. The AUC of propafenone enantiomers in *10/*10 is about 1.5-2 times of that of *1/*10 group or *1/*1 group, a

关键词： propafenone pharmacokinetics CYP2D6~* 10B genotype phenotype enantiomers

A Novel Multiplex Assay Wth 5 sTRIP Markers For screening And Diagnosing 22q11.2 Deletion

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A Novel Multiplex Assay Wth 5 STRIP Markers For Screening An...

spiral multi-phase CT in evaluating resectability of pancreatic carcinoma

第六届全国优生科学大会

作者： Long Yi~(1*) Zhengfeng Xu~2 Xuming Mo~3 , Yali Hu~4 Dongjin Wang~4 Bing Han~1 Yong Wang~1 Chi Yang~1 Yongzhong Jiang~3 Li shen~3 Xiaojun Zhou~1 ~1department of Pathology,nanjing University Medical school,nanjing 210093,china ~2Center of prenatal Diagnosis,nanjing Maternity and Child Health hospital,nanjing 210029 china ~3department of cardiothoracic surgery,nanjing children’s hospital,nanjing 210008,china ~4department of Obstetrics and Gynecology,department of cardiothoracic surgery of Drum Tower hospital, nanjing University Medical school,nanjing 210008,china

Clinical and prognostic diagnosis of the 22q11.2 deletion is important since the deletion has diverse medical complications and possible hereditary implications for close ***-based genotyping of short tandem repeat polymorphic (sTRP)marker is a common and powerful means of detecting *** have developed a multiplex assay with 5 new tetranucleotide or pentanucleotide sTR markers,22D,22D,22D,22D and D22s873 in the proximal region of 22q11.2 *** ascertain that the assay could be used as qualified tools,we performed genotyping analysis on 200 unrelated individuals from Chinese Han population,120 CHD patients and their unaffected *** data showed that the 5 markers met Hardy-Weinberg expectations and are *** using the the assay,8 of 120 CHD patients were determined to have a deletion within chromosome *** with dinucleotide markers,tetranucleotide or pentanucleotide markers produce less intensive stutter band and have no artificial multiband *** amplification results from the 5 new sTRP markers are unambiguous and easier to interpret in *** study demonstrated that the assay with the 5 markers were efficient and reliable and indicated that genotyping using tetranucleotide sTRP markers is an alterative approach to detect the deletion on chromosome 22q 11.2 in clinical diagnosis and genetic consultation.

关键词： 22q11.2 deletion congenital heart defect sTRP marker

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Hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 614-619页

作者： Qing-Juan Huang Qing Xu Xiao-Ning Wang Lian-Liang Zhang the department of Radiology, First Affiliated hospital, nanjing Medical University, nanjing 210029, china department of Radiology First Affiliated Hospital Nanjing Medical University Nanjing 210029 China. xuqingst@***

Objectives: To evaluate the specific manifestations of pancreatic carcinoma on spiral multi-phase CT and its resectability before operation. Methods: Ninety-seven patients were confirmed oper- atively and pathologically. Enhanced CT scan was performed with intravenous injection bolus of ap- proximately 75-120 ml (1-1.5 ml/kg body weight) contrast medium at a rate of 2.5-3 ml/s. In 68 pa- tients receiving dual-phase scan, the delayed scan time of arterial and venous phases was 18-20 s and 60-70 s, respectively, and in 29 patients receiving three-phase scan, the delayed scan time of arterial, pancreatic and portal venous phases was 18 s, 40 s and 75 s, respectively, with a slice of 3-5 mm thick- ness, a pitch of 1-1.5, and a reconstruction interval of 2.5-4.8 mm. Results: Positive and negative predictive values of un- resectable tumors were 97.65% and 75.86%, respec- tively. The sensitivity and accuracy were 90.67% and 90. 72%, respectively. Positive predictive values of dual-phase and three-phase were 95.83% and 100%, respectively; negative predictive values were 75% and 77.78%, respectively. Conclusions: spiral multi-phase CT is superior in re- vealing the involvement of peripancreatic vessels, the invasion of the neighboring organs, the size, shape and range of carcinoma, and the metastasis of liver and lymph node. The predictability of resection is obviously increased for patients with pancreatic car- cinoma.

关键词： pancreatic carcinoma surgery spiral computed tomography (CT) enhanced scan