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cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)

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Progress in Natural science:Materials International 2009年第7期19卷 837-844页

作者： Weijun Mai a,b, Chaoqun Hu a, a Applied Marine Biology Laboratory, South china Sea Institute of Oceanology, science academy of china, Guangzhou 510301, china b graduate university of chinese academy of sciences, beijing 100049, china [a]Applied Marine Biology Laboratory South China Sea Institute of Oceanology Science Academy of China Guangzhou 510301 China [b]Graduate University of Chinese Academy of Sciences Beijing 100049 China

The gene coding for lysozyme in blue shrimp (Litopenaeus stylirostris) was cloned, sequenced and expressed in pET-32a vector. The deduced amino acid sequence of F. merguiensi lysozyme showed 37–93% similarity with the mouse, human, chicken, and tiger prawn counterparts. The lysozyme was purified to homogeneity and observed as a band of approximately 15 kDa in 15% SDS–PAGE. Semiquantitative RT-PCR analysis demonstrated that mRNA transcripts of lysozyme could be mainly detected in the tissues of haemocytes, gill, gonad and the lymphoid organ of unchallenged shrimps, whereas the expression of lysozyme transcripts was increased in all the tested tissues after the heat-killed Vibrio alginolyticus challenge. The temporal expression of lysozyme mRNA in haemolymph challenged by Micrococcus luteus and V. alginolyticus was both up-regulated and reached the maximum level at 8 and 16 h post-stimulation, respectively, and then dropped back to the original level. Bacteriolytic activity of the lysozyme against different bacterial cultures was determined by the solid phase and turbidimetric assays. The results demonstrated that the lysozyme we obtained was not only against Grampositive and Gram-negative bacteria but also against shrimp pathogens V. alginolyticus and V. parahemolyticus. In addition, the study of the inhibition mechanism revealed that the antibacterial activity of the lysozyme was a result of the bactericidal effect.

关键词： Lysozyme C Litopenaeus stylirostris PCR-cloning Expression Antibacterial activity

Evolution of desertification in a two-dimensional energy balance model coupled with thermodynamics and dynamics

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science china Earth sciences 2010年第11期53卷 1726-1733页

作者： CHAO JiPing1,2 & LI YaoKun3,4 1National Marine Environmental Forecasting Center, beijing 100081, china 2First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, china 3State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics, Institute of Atmospheric Physics, chinese academy of sciences, beijing 100029, china 4graduate university of chinese academy of sciences, beijing 100049, china 1. National Marine Environmental Forecasting Center Beijing 100081 China2. First Institute of Oceanography State Oceanic Administration Qingdao 266061 China3. State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China4. Graduate University of Chinese Academy of Sciences Beijing 100049 China

The relationship between desert evolution and change in albedo has been investigated quasi-analytically using a zonal mean two-dimensional energy balance model which considers the radiation transmission process due to thermodynamics and bound- ary layer movement caused by kinetics. A climate state including temperature, zonal wind, meridional wind and vertical wind can be simulated according to the current zonal distribution of albedo. Given desert distribution, characterized by the value and distribution of albedo, the response of climate on albedo has been studied to analyze the evolution of desert climate. One significant result is that the simple model can reproduce mean meridional circulation. Another result indicates that climate corresponds to two equilibria. This happens when the junction temperature between vegetation and desert is higher than a certain critical value. As for the first equilibrium, the desert belt is predicted to move southward in the northern hemisphere with the increasing values of albedo, which corresponds to the current trend of climate change. For the second equilibrium, vegetation will expand northward with increasing values of albedo, which would indicate a narrowing of the desert belt. In order to determine if the two equilibria exist, new physical models are needed.

关键词： two-dimensional energy balance model desertification two equilibria

Interannual variability of Antarctic Oscillation and its influence on East Asian climate during boreal winter and spring

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science china Earth sciences 2006年第5期49卷 554-560页

作者： FAN Ke1,2,3 & WANG Huijun2 1. Nansen-Zhu International Research Center, Institute of Atmospheric Physics, chinese academy of sciences, beijing 100029, china 2. Department of Atmospheric science, Yunnan university, Kunming 650091, china 3. graduate university of the chinese academy of sciences, beijing 100049, china 1. Nansen-Zhu International Research Center Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China 2. Department of Atmospheric science Yunnan University Kunming 650091 China 3. graduate university of the chinese academy of sciences Beijing 100049 China

The interannual variability of Antarctic Oscillation (AAO) and its influence on East Asian climate during both boreal winter and spring are addressed. The results show that the positive AAO anomaly decreases the cold activity over East Asia during both boreal winter and spring. AAO-related barotropic meridional teleconnection from Antarctic to Arctic is found through analysis of mean me- ridional circulations. This meridional teleconnection is remarkable over Eurasia during boreal winter and over the Pacific Ocean during boreal spring. The results also show that zonal mean zonal wind at high latitudes in Southern Hemisphere has well positive correlation with that of Eurasia during boreal winter and has negative correlation with Pacific North American teleconnection (PNA) during boreal spring, which again display the meridional teleconnection. Thus, local meridional teleconnection is a possible linkage for interaction of circulations at mid-high latitudes between both hemispheres.

关键词： Antarctic Oscillation, Eurasian west wind, meridional teleconnection, mean meridional circulation.

Evidence of a two-component jet in the afterglow of GRB 070419A

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science china(Physics,Mechanics & Astronomy) 2010年第S1期53卷 265-266页

作者： ZHENG WeiKang1,2 & DENG JinSong1 1 National Astronomical Observatories,chinese academy of sciences,beijing 100012,china 2 graduate university of chinese academy of science,beijing 100049,china 1. National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China 2. graduate university of chinese academy of science Beijing 100049 China

A two-component jet model is proposed to explain the unusual afterglow of GRB *** the optical light curve,a wide "jet" with an opening angle of >30-40 degrees is assumed to produce the late shallow decay,while the three early power-law segments must be caused by a narrow jet with an opening angle of-2-4 *** energy injections to both components are *** X-ray emission may come from either the wide jet or the narrow *** the latter is correct,the jets may run into an ISM environment and the temporal index of the late energy injection may be q-0.65.

关键词： gamma-rays:bursts gamma-rays:observations

Simulations for terrestrial planets formation

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 794-803页

作者： ZHANG Niu 1,2 &JI JiangHui 2,3 1graduate university of chinese academy of sciences,beijing 100049,china 2Purple Mountain Observatory,chinese academy of sciences,Nanjing 210008,china 3 National Astronomical Observatories,chinese academy of sciences,beijing 100012,china graduate university of chinese academy of sciences Beijing 100049 China Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China

In this paper,the formation of terrestrial planets in the late stage of planetary formation is investigated using the two-planet *** that time,the protostar formed for about 3 Ma and the gas disk *** the model,the perturbations from Jupiter and Saturn are *** of the mass of outer planet,and the initial eccentricities and inclinations of embryos and planetesimals are also *** results show that,terrestrial planets are formed in 50 Ma,and the accretion rate is about 60%-80%.In each simulation,3-4 terrestrial planets are formed inside"Jupiter"with masses of 0.15 -3.6M⊕.In the 0.5-4 AU,when the eccentricities of planetesimals are excited,planetesimals are able to accrete material from wide radial *** plenty of water material of the terrestrial planet in the Habitable Zone may be transferred from the farther places by this *** could also happen a few times between two major planets only if the outer planet has a moderate mass and the small terrestrial planet could survive at some resonances over time scale of 10 8 *** one of our simulations,commensurability of the orbital periods of planets is very ***,a librating-circulating 3:2 configuration of mean motion resonance is found.

关键词： astrophysics exoplanet planetary formation n-body simulation

Optimization of mathematical models for thematic maps

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science china(Technological sciences) 2010年第S1期53卷 15-24页

作者： QI QingWen1,ZHANG An1,JIANG LiLi1,ZOU XiuPing2,XU Li3 & CHENG Xi1,4 1 Institute of Geographical sciences and Natural Resources Research,chinese academy of sciences,beijing 100101,china 2 Institute of Policy and Management,chinese academy of sciences,beijing 100080,china 3 Taishan university,Taian 271021,china 4 graduate university of chinese academy of sciences,beijing 100049,china chinese Acad Sci Inst Geog Sci & Nat Resources Res Beijing 100101 Peoples R China chinese Acad Sci Inst Policy & Management Beijing 100080 Peoples R China Taishan Univ Tai An 271021 Shandong Peoples R China chinese Acad Sci Grad Univ Beijing 100049 Peoples R China

The thematic map is a major class of maps designed to demonstrate particular features or concepts,functioning as an indispensable tool in geographical *** process of thematic mapping is one into which geographical research goes deeply and *** key activity and course of thematic map production is the use of mathematical models to create thematic data ***,the selection and optimization of mathematical models is in the forefront of thematic map *** theoretical foundations,mechanisms and methods of mathematical model optimization are expounded in this paper,including two approaches,the phase by phase mode and the multi-aim scheme balance *** studies in eco-environment mapping and emergency mapping are described and analyzed,with a hierarchical analysis method being used in the model optimization for eco-environment fragility and sensitivity assessment mapping in Beibuwan (Guangxi) District,the dynamic system (DS) method being used in the model optimization for ecological security adjustment mapping in Xishuang Banna,Yunnan province,and the multi-phase mode being used in the models for forest fire and infectious diseases mapping.

关键词： thematic map mathematic model optimization

Multiparty simultaneous quantum identity authentication with secret sharing

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science china(Physics,Mechanics & Astronomy) 2008年第3期51卷 321-327页

作者： YANG YuGuang1,2,WEN QiaoYan3 & ZHANG Xing4,5 1 College of Computer science and Technology,beijing university of Technology,beijing 100022,china 2 State Key Laboratory of Information Security (graduate university of the chinese academy of sciences),beijing 100049,china 3 School of science,beijing university of Posts and Telecommunications,beijing 100876,china 4 Institute of Electronic Technology,Information Engineering university,Zhengzhou 450004,china 5 Trusted Computing Laboratory,beijing university of Technology,beijing 100022,china College of Computer science Technology Beijing University of Technology Beijing 100022 China State Key Laboratory of Information Security [graduate university of the chinese academy of sciences] Beijing 100049 China School of science Beijing University of Posts Telecommunications Beijing 100876 China Institute of Electronic Technology Information Engineering University Zhengzhou 450004 China Trusted Computing Laboratory Beijing University of Technology Beijing 100022 China

Two multiparty simultaneous quantum identity authentication (MSQIA) protocols based on secret sharing are presented. All the users can be authenticated by a trusted third party (TTP) simultaneously. In the first protocol,the TTP shares a random key K with all the users using quantum secret sharing. The ith share acts as the authentication key of the ith user. When it is necessary to perform MSQIA,the TTP generates a random number R secretly and sends a sequence of single photons encoded with K and R to all the users. According to his share,each user performs the corresponding unitary operations on the single photon sequence sequentially. At last,the TTP can judge whether the impersonator exists. The sec-ond protocol is a modified version with a circular structure. The two protocols can be efficiently used for MSQIA in a network. They are feasible with current technol-ogy.

关键词： quantum identity authentication secret sharing quantum cryptography

Improvement of a 3D radar backscattering model using matrix-doubling method

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science china Earth sciences 2010年第7期53卷 1029-1035页

作者： NI WenJian1,2, GUO ZhiFeng1 & SUN GuoQing3 1 State Key Laboratory of Remote Sensing science, jointly sponsored by the Institute of Remote Sensing Applications of chinese academy of sciences and beijing Normal university, beijing 100101, china 2 graduate university of chinese academy of sciences, beijing 100049, china 3 Department of Geography, university of Maryland, College Park, MD 27042, USA 1. State Key Laboratory of Remote Sensing science jointly sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University Beijing 100101 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China3. Department of Geography University of Maryland College Park MD 27042 USA

Radiative transfer models have been widely used to simulate the radar backscattering from forested areas. A three-dimensional radar backscatter model of forest canopy developed in previous studies takes full account of spatial position of trees in a forest stand, and the interactions among crown, trunk and ground surface. The model predicted well for the co-polarized backscatter measurements, but underestimated the backscattering for cross-polarization, primarily because only the first-order scattering within tree crowns was considered in the model. The backscattering at cross-polarization depends strongly on multiple scatter- ing within tree crowns. To produce good estimations for cross-polarized component, the matrix-doubling method is employed here to compute multiple-scattering within the crown. The modified model is compared with the original model, and the field forest measurements and AIRSAR data are used for validation of the modified model. The cross-polarization backscattering is improved in different degrees for different crown structures and at different bands.

关键词： remote sensing forestry electromagnetic propagation radar backscattering model matrix-doubling

Research on the method for retrieving soil moisture using thermal inertia model

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science china Earth sciences 2006年第5期49卷 539-545页

作者： LIU Zhenhua1 & ZHAO Yingshi2 1. Information College, South china Agricultural university, Guangzhou 510642, china 2. graduate university of chinese academy of sciences, beijing 100049, china 1. Information College South China Agricultural University Guangzhou 510642 China 2. graduate university of chinese academy of sciences Beijing 100049 China

In order to improve accuracy of soil moisture inversion using remote sensing, a new thermal inertia model is proposed in this paper. The improved model needs only surface maximum temperature as the temperature parameter input instead of input of the surface temperature difference, as well as the surface sensible and latent fluxes are introduced into boundary conditions of thermal conductivity equation. Furthermore, surface soil conductive heat transfer equation of two-layer model is used to solve the soil thermal inertia so that the remote sensing thermal inertia method can be ap- plied to regions with better-covered vegetation, but usually only for the bare areas or worse vegetation covered areas. The model has been tested at several locations in the area of west Inner Mongolia. Comparing the simulation of the new model with the measurements obtained by apparent thermal inertia and by field test, the result shows that the inertia thermal model can be used to estimate soil moisture in more reasonable accuracy.

关键词： thermal model, thermal conductivity equation, soil initial, sensible flux, latent flux, two-layer model.