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Antioxidant properties of berberine on cultured rabbit corpus cavernosum smooth muscle cells injured by hydrogen peroxide

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Acta Pharmacologica Sinica 2007年第12期28卷 1914-1918页

作者： Yan TAN~(2,3,5),Qiang TANG~4,Ben-rong HU~4,Ji-zhou XIANG~4~2 Institute of Urology,Renmin Hospital,Yunyang Medical College,Shiyan 442000 China,~3 Department of Pharmacology,Yunyang MedicalCollege,Shiyan 442000,China,~4 Department of Pharmacology,Tongji Medical College,Huazhong University of Science and Teehnology,Wuhan 430030,China Institute of Urology Renmin Hospital Yunyang Medical College Shiyan China Department of Pharmacology Yunyang Medical College Shiyan China Department of Pharmacology Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the antioxidant properties of berberine (Ber) on corpuscavenosum smooth muscle cells (CCSMC) in penile erectile ***:We examined the effects of Ber on cultured rabbit CCSMC damaged by hydrogenperoxide (H2O2) through examining cell viability by methyl thiazolyl tetrazoliumassay and assessing the level of malondialdehyde (MDA),superoxide dismutase,(SOD) activity,nitric oxide (NO) products,and lactate dehydrogenase (LDH) re-lease in cells after stimulation with ***:Treatment with 1 mmol/L H2O2significantly decreased the cell viability,NO products,and SOD activity of CCSMCfrom 100% to 48.57%±4.1% (P<0.01),66.8±16.3 to 6.7±2.1 μmol/L (P<0.01),and49.5±1.8 to 30.1±2.6 U/mL (P<0.01),respectively,and increased LDH release andMDA content from 497.6±69.5 to 1100.5±56.3 U/L (P<0.01) and 3.7±1.3 to 78.4±2.9nmol/mg protein (P<0.01),***,treatment with different con-centrations of Ber (10-1000 μmol/L) inhibited the damaging effects of H2O2,withincreased cell viability (P<0.05 or P<0.01),NO production (P<0.01),and SODactivity (P<0.01) and decreased LDH release and MDA content (both P<0.01).Conclusion:Bet could produce its antioxidant action on oxidative stress-inducedcultured *** effects may be of benefit in the prevention of penileerectile dysfunction.

关键词： berberine antioxidant action corpus cavernosum erectile dysfunction

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Evolutionary Graph Drawing Algorithms

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Wuhan University Journal of Natural Sciences 2003年第S1期8卷 212-216页

作者： Huang Jing-wei, Wei Wen-fangSchool of Computer, Wuhan University, Wuhan 430072, Hubei, ChinaComputer Center, Yunyang Medical College, Shiyan 442000, Hubei, China School of Computer Wuhan University Wuhan Hubei China Computer Center Yunyang Medical College Shiyan Hubei China

In this paper, graph drawing algorithms based on genetic algorithms are designed for general undirected graphs and directed graphs. As being shown, graph drawing algorithms designed by genetic algorithms have the following advantages: the frames of the algorithms are unified, the method is simple, different algorithms may be attained by designing different objective functions, therefore enhance the reuse of the algorithms. Also, aesthetics or constrains may be added to satisfy different requirements.

关键词： graph drawing algorithms genetic algorithms

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Roles of nitric oxide in protective effect of berberine in ethanol-induced gastric ulcer mice

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Acta Pharmacologica Sinica 2005年第11期26卷 1334-1338页

作者： Long-tui PAN~(2,3), Qiang TANG~2, Qin FU~2, Ben-rong HU~2, Ji-zhou XIANG~(2,4), Jia-qing QIAN~2 ~2 Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China ~3 Department of Pharmacology, Yunyang Medical College, Shiyan 442000, China Department of Pharmacology Tongji Medical College of Huazhong University of Science and Technology Wuhan China Department of Pharmacology Yunyang Medical College Shiyan China

Aim: To investigate the protective effects of berberine on ethanol-induced gastric ulcer in mice. Methods: Gastric ulcers were induced by oral ingestion of ethanol. Nitric oxide (NO) content was measured, and mRNA expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results: The ulcer index (UI) at 1 h, 2 h, 3 h and 6 h after oral administration of ethanol was 23.8■1.4,23.3■2.2,22.3■1.2 and 20.8■1.1, respectively. The UI in the berberine-treated groups (5 mg/kg and 50 mg/kg) was less than the control group. The content of NO in the control group was 73.3+7.3 μL/L, 94.0■9.2 μL/L, 109.6■ 6.4 μL/L and 138.2■10.2 μU/L in gastric juice and 5.8■1.1 μmol/g protein, 8.3■ 1.1 μmol/g protein, 9.8■ 1.1 μmol/g protein and 11.9■ 1.2 μmol/g protein in gastric tissue at 1 h, 2 h, 3 h and 6 h, respectively, after the oral administration of ethanol. The content of NO in the berberine-treated groups (5 mg/kg and 50 mg/kg) was higher than the control group at 1 h after the oral administration of ethanol (P<0.05), and was lower at 6 h (P<0.05). Analysis by RT-PCR showed that expression of eNOS was inhibited but iNOS expression was enhanced by ethanol. However, the expression of eNOS could be enhanced and iNOS expression could be inhibited by berberine (P<0.01). Conclusion: Berberine could significantly protect gastric mucosa from damage by ethanol. This effect may be related to the increased expression of eNOS mRNA and inhibited expression of iNOS mRNA.

关键词： berberine nitric oxide protective agents ethanol-induced disorders stomach ulcer

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血平板针尖样及云雾状菌落分离与鉴定

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微生物学通报 1998年第3期25卷 153-156页

作者：邓维秀郧阳医学院微生物学教研室十堰442000

采集98份临床标本接种血平板培养，40份生长出针尖样及云雾状菌落。经分离鉴定38株为细菌L型，其中金黄色葡萄球菌（Staphyloeoccusaureus）居首位，占52．1％（20／38）．其返视性与L型平板分离相比较差。但对抗生素的敏感性则无差别... 详细信息

采集98份临床标本接种血平板培养，40份生长出针尖样及云雾状菌落。经分离鉴定38株为细菌L型，其中金黄色葡萄球菌（Staphyloeoccusaureus）居首位，占52．1％（20／38）．其返视性与L型平板分离相比较差。但对抗生素的敏感性则无差别。表明血平板可用于分离细菌L型，并保持了传统L型的特性。

关键词：血平板针尖样云雾状菌落鉴定

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发展武当山生态旅游的探讨

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生态经济 2001年第12期17卷 132-134页

作者：王友安湖北省十堰市环境科学研究所 442000

本文在分析武当山概况、现状的基础上 ,得出了发展生态旅游是武当山旅游业的唯一出路。进而分析了武当山的环境容量 ,提出了发展武当山生态旅游的对策。

关键词：武当山生态旅游环境容量发展对策

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Study on HBV Vertical Transmission via the in vitro Fertilization (IVF) Technique

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Journal of Reproduction and Contraception 2009年第2期20卷 73-79页

作者： Jing-ning YANG Qing-bin LUO Chang-jun ZHANG Hua WANG Research Center of Reproductive Medicine the Affiliated People's Hospital Yunyang Medical College Shiyan 442000 China Department of lmmunology Yunyang Medical College Shiyan 442000 China

Objective To study the hepatitis B virus （HBV） vertical transmission via infected spermatozoa. Methods Eighteen male patients with HBV infection who underwent in vitro fertilization （IVF） were studied, 5 HBV negative patients were selected as the control. Fluorescence in situ hybridization （FISH） analysis using the partial-length HBV DNA as the hybridization probe was performed to explore the existence of HBV DNA in the sperm and in the host embryonic genome. Results FISH showed that 5 of 18 patients＇ sperm presented positive signals and 2 of 18 embryos presented positive signals, while no positive signals were found in control group. Conclusion The HBV DNA was found in human sperm and embryos of HBV patients. These results provide direct evidence that HBV DNA could transmit to foetus via human infected spermatozoa.

关键词： hepatitis B virus transmission human spermatozoa embryo in vitro fertilization

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创新教育背景下的中学历史教育教学要求

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学科教育 2002年第2期 27-30页

作者：韩飞十堰市教学研究室湖北十堰442000

本文主要论述了创新教育背景下的中学历史教育教学的四大要求 :教育观念的要求、历史教师素质的要求、教育教学目标要求和教育教学方法要求。目的是为了探索历史教育教学改革的方向。

关键词：创新教育中学教育历史教学教育观念教学方法教学目标

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组织工程学在生物人工肾小管研究中的应用

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生物医学工程学杂志 2002年第1期19卷 144-147页

作者：张永张建鄂湖北省十堰市太和医院肾病内科十堰442000

组织工程学是材料学、工程学和生命科学的结合 ,组织工程学已被成功应用于正在研制的生物人工肾小管辅助装置 (RAD)中。实验证实该装置能够替代急慢性肾功衰竭患者丧失的肾小管转运、代谢和内分泌功能。

关键词：生物人工肾小管组织工程学肾功能衰竭细胞治疗

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Phosphoinositide 3-kinase and p38 MAPK and ERK1/2 are involved in EGF-induced vascular smooth muscle cells proliferation

Phosphoinositide 3-kinase and p38 MAPK and ERK1/2 are involv...

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2008年全国生物流变学与生物力学学术会议

作者： LongJu-Chen~1,FuGui-Zhang~2,Jiang Hua~3,HanQin-Wang~(1,4)(1.Department of Anatomy,Yunyang Medical College,ShiYan,HuBei 442000 2.Department of Cardiology,Affiliated Union Hospital of Tongji Medical College,HuazhongUniversity of Science and Technology,Wuhan,430022 3.Department of Blood,Yunyang Medical College Affriliated Taihe Hospital,ShiYan,HuBei 442000 4.Institute of life scince,Yunyang Medical College Affriliated Taihe Hospital,ShiYan,HuBei 442000)

Objective:The proliferation of vascular smooth muscle cells(VSMCs) is the basis of many kinds of vascular disease including artherosclerosis (AS) and high blood pressure(HBS) and restenosis after injury of blood vessel as well as ***,epidermal growth factor(EGF) is a potent angiogenic *** has demonstrated that EGF can induce proliferation of ***,the cell

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论探究式物理教学

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陕西师范大学学报（自然科学版） 2003年第Z1期31卷 227-229页

作者：龚乃新东风四中湖北十堰442000

根据物理教学以及素质教育的要求 ,对实施探究式物理教学作出分析。

关键词：自行探究探究能力创设情景

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