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A novel multi-functional cell-based microphysiometer

Progress in Natural Science:Materials International 2006年第8期16卷 808-816页

作者： XU Ying, XU Gaixia, LIU Qingjun, CAI Hua, LI Yan, LI Rong and WANG Ping (Biosensor National Special laboratory, key laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China state key laboratory of transducer technology, chinese academy of sciences, shanghai 2.0050, China) [a]Biosensor National Special laboratory Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou China [b]state key laboratory of transducer technology Chinese Academy of Sciences Shanghai China

This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS (multi-light addressable potentiometric sensor). In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H+, Na+, K+, Ca2+ under the effects of western medicines (dilantin, phenobarbital sodium) and chinese drugs (scutellaria, medlar, hemlock parsley) were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types (cardiac myocytes and neurons) could be measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs.

关键词： multi-functional microphysiometer cell-based biosensor cellular ion concentration measurement cellular action potential.

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Research progress in terahertz quantum cascade lasers

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Science China(Information sciences) 2012年第1期55卷 16-26页

作者： CAO JunCheng key laboratory of Terahertz Solid-state technology,shanghai Institute of Microsystem and Information technology,chinese academy of sciences,shanghai 2.0050,China 1. key laboratory of Terahertz Solid-state technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China

The terahertz(THz)quantum cascade laser(QCL)is a coherent THz source based on intersubband transitions of unipolar *** semiconductor lasers,THz QCLs are solid-state,electrically pumped,tunable,and can be easily *** paper reviews the state of the art for the design,operation temperature,and output power of THz *** applications in THz communication and imaging are also discussed.

关键词： terahertz quantum cascade laser communication imaging

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Magnetic and Structural Properties in Co/Cu/Co Sandwiches with Ni and Cr Buffer Layers

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Journal of Materials Science & technology 2000年第2期16卷 195-196页

作者： Hollglie SHEN, Tie LI, Qinwo SHEN, Qiang PAN and Shichang ZOU (state key laboratory of Functional Materials for Informations and state key Laboratories of transducer technology, shanghai Institute of Metallurgy, chinese academy of sciences, shanghai 2000 state key laboratory of Functional Materials for Informations Shanghai Institute of Metallurgy Chinese Academy of Sciences Shanghai 200050 China

The magnetic and structural properties in Co/Cu/Co sandwiches with Ni and Cr buffer layers were investigated. It was found that the coercivity in Ni layer buffered samples decreases with increasing Ni layer thickness, while that in Cr layer buffered ones increases with increasing Cr layer thickness, leading to a large difference in field sensitivity of their giant magnetoresistance (GMR) properties. X-ray diffraction and high resolution transmission electron microscope images exhibited that there is a strong fcc (111) texture in the samples with Ni buffer layer. But there are only randomly oriented potycrystalline grains in Cr buffered sandwiches. According to atomic force microscope topography, the surface roughness of Cr buffered sandwiches is smaller than that of Ni buffered ones. It is demonstrated that buffer layer influences both magnetic and structural properties in Co/Cu/Co sandwiches as well as their GMR characteristics.

关键词： Cr Co HRTEM Magnetic and Structural Properties in Co/Cu/Co Sandwiches with Ni and Cr Buffer Layers Cu Ni

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Effects of deposition parameters on the properties of VO2 thin films

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Progress in Natural Science:Materials International 2006年第11期16卷 1193-1197页

作者： WANG Lixia, LI Jianping*, GAO Xiaoguang and HE Xiuli (state key laboratory of transducer technology, Institute of Electronics, chinese academy of sciences, Beijing 100080, China) [a]state key laboratory of transducer technology Institute of Electronics Chinese Academy of Sciences Beijing China

The vanadium oxide thin films are deposited for microbolometers by radio frequency reactive sputtering method at room temperature. The effects of the oxygen partial pressure on the deposition rate, electrical properties and compositions of the films are discussed. The as-deposited VOx thin films with x value of nearly 2 are deposited by adjusting the oxygen partial pressure. After oxidation annealing of these films in air, the VO2 films with high temperature coefficients of resistivity （about -4%/℃） and low resistivity can be obtained. The square resistances of the films are in the range of 100 kΩ/squ—300 kΩ/squ. All films are deposited at room temperature and annealed at 400℃, in which the compatibility between VOx deposition process and MEMS （micro electromechanical systems） is greatly improved.

关键词： vanadium dioxide temperature coefficients of resistivity oxygen partial pressure RF reactive sputtering

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Progress in glial cell studies in some laboratories in China

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Science China(Life sciences) 2010年第3期53卷 330-337页

作者： DUAN ShuMin Institute of Neuroscience,state key laboratory of Neuroscience,shanghai Institutes for Biological sciences,chinese academy of sciences,shanghai China,200031 1. Institute of Neuroscience State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China 200031

Glial cells in the central nervous system(CNS) consist of a heterogeneous population of cell types,each characterized by distinct morphological features,physiological properties,and specific *** contrast to the previous view that glial cells were passive elements in the brain,accumulating evidence suggests that glial cells are active participants in various brain functions and brain *** review summarizes recent progress of glial cell studies from several groups in China,ranging from studies about the mechanisms of neuron-glia crosstalking to investigations on the roles of glial cells in various CNS disorders.

关键词： neuron-glia interaction ATP plasticity pain ischemia neural injury and repair

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Synapse formation and remodeling

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Science China(Life sciences) 2010年第3期53卷 315-321页

作者： LUO ZhenGe Institute of Neuroscience and state key laboratory of Neuroscience,shanghai Institutes for Biological sciences,chinese academy of sciences,shanghai 200031,China 1. Institute of Neuroscience and state key laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Synapses are specialized structures that mediate information flow between neurons and target cells,and thus are the basis for neuronal system to execute various functions,including learning and *** are around 1011 neurons in the human brain,with each neuron receiving thousands of synaptic inputs,either excitatory or inhibitory.A synapse is an asymmetric structure that is composed of pre-synaptic axon terminals,synaptic cleft,and postsynaptic *** formation involves a number of cell adhesion molecules,extracellular factors,and intracellular signaling or structural *** the establishment of synaptic connections,synapses undergo structural or functional changes,known as synaptic plasticity which is believed to be regulated by neuronal activity and a variety of secreted *** review summarizes recent progress in the field of synapse development,with particular emphasis on the work carried out in China during the past 10 years(1999-2009).

关键词： synapse development neuronal activity synaptic plasticity

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Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 673-680页

作者： Chun-Hui HOU, Jian HUANG, and Ruo-Lan QIAN* state key laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 200031, China state key laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3028 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3004 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA

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Pain research in China

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Science China(Life sciences) 2010年第3期53卷 356-362页

作者： ZHANG Xu Institute of Neuroscience,state key laboratory of Neuroscience,shanghai Institutes for Biological sciences,chinese academy of sciences,shanghai 200031,China 1. Institute of Neuroscience State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

In addition to investigating the anatomy,neurochemistry and neurophysiology of pain pathways,chinese researchers have extended their work into the molecular and cellular mechanisms of sensory afferent transmission at the spinal cord level as well as cognitive processing in the *** mechanism underlying acupuncture analgesia remains a subject of special interest for chinese pain researchers,with the aim of combining clinical practice with the understanding of pain transmission and analgesic mechanism.

关键词： dorsal root ganglion spinal cord nociception receptors ion channels gene expression

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An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 571-576页

作者： Li-Qing XIAO, Ai-Hua LIU, and Yong-Lian ZHANG state key laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 200031, China state key laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family.

关键词： Bin1b epididymis β-defensin double-copy protein polyclonal antiserum

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<100> Textured Diamond Film on Silicon Grown by Hot Filament Chemical Vapor Deposition

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Journal of Materials Science & technology 1995年第6期11卷 426-428页

作者： Xuanxiong ZHANG Tiansheng SHI and Xikang ZHANG (state key laboratory of Functional Materials for Informatics, shanghai Institute of Metallurgy,chinese academy of sciences, shanghai, 2.0050, China) shanghai Inst of Metallurgy Chinese Acad of Sciences Shanghai China

The textured growth of diamond film on HF eroded silicon wafer has been studied by HFCVD. The evolution of grain size and sudece morphology vs deposition time is presented and the textured thick diamond film (80μm)... 详细信息

关键词： Textured Diamond Film on Silicon Grown by Hot Filament Chemical Vapor Deposition OO

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