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Down-regulation of C-type natriuretic peptide receptor by vasonatrin peptide in cardiac myocytes and fibroblasts

Acta Pharmacologica Sinica 2004年第4期25卷 26-32页

作者： Shun-yan Lü, Miao-zhang ZHU2, Dian-shi WANG3, Jun YU, Hai-tao GUO, Yu-zhen HU, Qi-ming WEI4 Department of Physiology, Departmentof Anatomy, Fourth Military Medical university, Xi-an 710032, China 4.ardiorenal 3 molecular laboratory, university of maryland school of medicine, baltimore21201, usa Department of Physiology Fourth Military Medical University Xi-an 710032 China

AIM: To investigate the regulatory effects of vasonatrin peptide (VNP) on the expression of C-type natriuretic peptide receptor (NPR-C) in cultured neonatal rat cardiac myocytes and fibroblasts. METHODS: Quantitative RT- PCR was undertaken to evaluate the levels of NPR-C mRNA and radioimmunoassay was used to determine the formation of intracellular cGMP. RESULTS: Twenty-four hours hypoxic exposure increased the level of NPR-C mRNA in cardiomyocytes, while did not alter the expression of NPR-C in cardiac fibroblasts. VNP (1×10-8-1×10-6 mol/L) reduced the levels of NPR-C mRNA in cardiac myocytes induced by hypoxia in a concentration-dependent manner, and with high concentration (1×10-6mol/L) also decreased the expression of NPR-C in cardiac fibroblasts and air-control cardiac myocytes. The inhibitory effects of VNP on the expression of NPR-C was mimicked by 8- bromo-cGMP 1×10-6 mol/L (a membrane permeable analog of cGMP). VNP (1×10-8-1×10-6mol/L) increased the formation of intracellular guanosine-3',5'-cyclic monophosphate (cGMP) in both cardiac myocytes and fibroblasts. HS-142-1, the particulate guanylyl cyclase-coupled receptor antagonist, partially abrogated the above effects of VNP. CONCLUSION: Hypoxic exposure for 24 h up-regulated the expression of NPR-C in cultured neonatal rat cardiac myocytes. VNP decreased the expression of NPR-C in cardiac myocytes and fibroblasts under both air- control and hypoxic condition, which was at least partially mediated by guanylate cyclase linked natriuretic peptide receptors through increasing the intracellular cGMP.

关键词： C-type natriuretic peptide myocardium fibroblasts hypoxia

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Microarray, SAGE and their applications to cardiovascular diseases

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Cell Research 2002年第2期12卷 105-115页

作者： SHUI QING YE, TERA LAVOIE, DAVID C USHER, LI Q. ZHANG1 Division of Pulmonary and Critical Care medicine, Johns Hopkins university, school of medicine, baltimore, MD 21224, usa2Department of Biological Science, university of Delaware, Newark, DE 19716, usa 1Division of Pulmonary and Critical Care medicine Johns Hopkins University School of Medicine Baltimore MD 21224 USA 1Division of Pulmonary and Critical Care medicine Johns Hopkins University School of Medicine Baltimore MD 21224 USA 2Department of Biological Science University of Delaware Newark DE 19716 USA 3Department of Surgery University of Maryland Baltimore MD 21201 USA

The wealth of DNA data generated by the human genome project coupling with recently invented high-throughput gene expression profiling techniques has dramatically sped up the process for biomedical researchers on elucidating the role of genes in human diseases. One powerful method to reveal insight into gene functions is the systematic analysis of gene expression. Two popular high-throughput gene expression technologies, microarray and Serial Analysis of Gene Expression (SAGE) are capable of producing large amounts of gene expression data with the potential of providing novel insights into fundamental disease processes, especially complex syndromes such as cardiovascular disease, whose etiologies are due to multiple genetic factors and their interplay with the environment. Microarray and SAGE have already been used to examine gene expression patterns of cell-culture, animal and human tissues models of cardiovascular diseases. In this review, we will first give a brief introduction of microarray and SAGE

关键词： Microarray SAGE cardiovascular diseases RNA gene expression profiling.

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Structural immunology of costimualtory and coinhibitory molecules

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Science China(Life Sciences) 2010年第2期53卷 183-189页

作者： WANG ShengDian1 & CHEN LiePing1,2 1Center for Infection and Immunity,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China 2Department of Oncology,Johns Hopkins university school of medicine,baltimore,maryland,usa 1. Center for Infection and Immunity Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China2. Department of Oncology Johns Hopkins University School of Medicine Baltimore Maryland USA

The T cell costimulatory pathways are central to regulating immune responses,and targeting these pathways represents one of the most promising approaches for achieving *** molecular structures of costimulation revealed invaluable mechanistic insights underlying costimulatory receptor/ligand specificity,affinity,oligomeric state,and valency,which provided the bases for better manipulation of these signaling *** incredible growth of this field led to identification of new members and unexpected interactions,revealing a complicated regulatory network of immune *** advances in structural biology of costimulation will promise unprecedented opportunities for furthering our understanding and therapeutic application of T cell costimulatory pathways.

关键词： costimulatory molecules T cells molecular structure immunotherapy

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Cell-specific Regulation of APOBEC3F by Interferons

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 297-304页

作者： Songcheng YING~1# Xuzhao ZHANG~1# Phuong Thi Nguyen SARKIS~2# Rongzhen XU~1 Xiaofang YU~(1,2*) 1 Second Affiliated Hospital,Cancer Institute,school of medicine,Zhejiang university,Hangzhou 310009,China 2 Department of molecular Microbiology and Immunology,Johns Hopkins BIoomberg school of Public Health,baltimore.maryland 21205,usa SecondAffiliatedHospital CancerInstituteSchoolofMedicineZhejiangUniversityHangzhou310009China DepartmentofmolecularMicrobiologyandImmunology JohnsHopkinsBloombergSchoolofPublicHealthBaltimoreMaryland21205USA

Human cytidine deaminase APOBEC3F（A3F）has broad anti-viral activity against hepatitis Bvirus and retroviruses including human immunodeficiency virus type ***,its regulation in viralnatural target cells such CD4+T lymphocytes,macrophages,and primary liver cells has not been *** we showed that A3F was up-regulated by interferon（IFN）-α in primary hepatocytes andmultiple liver cell lines as well as *** the IFN-α signaling pathway was active in Tlymphoid cells and induction of other IFN stimulated genes such as PKR was detected,A3F and APOBEC3G（A3G）were not induced by IFN-α in these ***,additional factors other than known IFN-stimulatedgenes also regulated IFN-α-induced A3F expression distinctly.A3F and A3G expression levels in primaryhepatocytes,especially after IFN-α stimulation,were comparable to those in CD4~ T lymphocytes in *** variations of A3F and A3G expression in primary hepatocytes from various subjectswere *** variations in A3F and/or A3G regulation and expression might influence the clinicaloutcomes of hepatitis B infection.

关键词： APOBEC3F interferon human immunodeficiency virus type 1 hepatitis B virus cytidine deaminase

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A FLUID MECHANICS MODEL OF TISSUE FLUID FLOW IN LIMB CONNECTIVE TISSUE——A MECHANISM OF ACUPUNCTURE SIGNAL TRANSMISSION

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Journal of Hydrodynamics 2009年第5期21卷 675-684页

作者： ZHANG Di YAO Wei DING Guang-hong YANG Jing SCHWARZ Wolfgang FEI Lun LIU Fang SHEN Xue-yong LAO Li-xing Shanghai Research Center of Acupuncture and Meridian Department of Mechanics and Engineering Science Fudan University Shanghai 200433 China Anatomy Department of the Second Medical university of PLA Shanghai 200433 China Acupuncture College Shanghai University of Traditional Chinese Medicine Shanghai 200032 China Center for Integrative medicine University of Maryland School of Medicine Baltimore MD 21201 USA

This article explores the mechanisms of acupuncture meridians by determining characteristics of the tissue fluid flow in the connective tissue along meridians. Based on deep dissection of acupoints on the upper and lower limbs of the human body and micro and macro observation and measurement, a mathematical model of the flow of tissue fluid in interosseous membranes is constructed. It is shown that the signal transmission along acupuncture meridians may be determined by unique anatomical and physiological factors that govern the flow of tissue fluid in the connective tissue and involve mast cell degranulation. Our results provide a preliminary explanation of the phenomenon of the ＂de qi＂ sensation and the mechanism of acupuncture signal transmission along acupuncture meridians.

关键词： acupuncture meridian tissue-fluid flow mast cell

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利用T4噬菌体展示猪瘟病毒E2抗原

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中国生物工程杂志 2004年第11期24卷 61-64页

作者：吴健敏任兆钧余兴龙涂长春张念祖解放军军需大学军事兽医研究所美国马里兰州大学医学院生物化学及分子生物研究中心巴尔的摩美国212011503 云南省热带亚热带动物病毒病开放实验室昆明650024

利用重组PCR技术将猪瘟病毒 (CSFV )E2蛋白主要抗原编码区基因 (mE2 )与T4噬菌体SOC基因融合 ,构建了大小为 643bp的SOC mE2融合基因 ,再将其插入携带T4溶菌酶基因 (e)和(denV)基因的T4重组载体 (PRH) ,构建了重组载体pRsmE2。通过重组... 详细信息

利用重组PCR技术将猪瘟病毒 (CSFV )E2蛋白主要抗原编码区基因 (mE2 )与T4噬菌体SOC基因融合 ,构建了大小为 643bp的SOC mE2融合基因 ,再将其插入携带T4溶菌酶基因 (e)和(denV)基因的T4重组载体 (PRH) ,构建了重组载体pRsmE2。通过重组载体与缺失突变型T4发生同源重组 ,可将SOC mE2融合基因整合入T4的基因组中 ,并成功地将大小约 2 1 5aaSOC mE2融合蛋白展示于T4噬菌体衣壳表面。经Westernblot、胶体金免疫电镜等免疫学检测证实 ,展示于T4表面的mE2融合蛋白具有CSFV免疫学活性。

关键词：重组载体融合基因抗原编码区融合蛋白噬菌体展示携带免疫学检测猪瘟病毒 CSFV 利用

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Anti-microtubule activity of tubeimoside I and its colchicines binding site of tubulin

Anti-microtubule activity of tubeimoside I and its colchicin...

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第九届全国中药和天然药物学术研讨会

作者： Rundi Ma~1 Gang Song~1 Wenbing You~1 Lijian Yu~(1*) Weiming Su~1 Mingneng Liao~1 Yongping Zhang~1 Laizhen Huang~1 Xiaoyu Zhang~(1,3) Tingxi Yu~(1,2) ~1Key laboratory of Marine Materia Medica,Guangdong Ocean university,Jiefang East Avenue 40,Xiashan,Zhanjiang 524025,China ~2Department of Biochemistry and molecular Biology,Public Health,Johns Hopkins university, baltimore,MD 21205,usa ~3Department of Otorhinolaryngology-Head and Neck Surgery,university of maryland school of medicine,baltimore,MD 21228,usa.The authors contributed equally to this work.

Background:Tubeimoside I(TBMS1)was isolated from the tubers of Bolbostemma paniculatum(Maxim.)***1 shows potent antitumor *** present study was conducted to investigate the antimicrotubule role of TBMS1 and its binding site of ***:Cell growth inhibition was measured by MTT after treatment with *** kinetics of TBMS1 by human nasopharyngeal carcinoma CNE-2Z cell line(CNE-2Z)was assayed by *** protein(MTP)was prepared from porcine brain through two cycles of polymerization-depolymerization in a high molarity *** of MTP polymerization induced by TBMSI was determined by a turbidity measurement and a sedimentation assay:the interactions of TBMS1 with tuhulin within CNE-2Z cells were investigated by immunofluorescence microscopy and ***1 was tested for its ability to inhibit binding of known tubulin ligands through competitive binding ***:TBMS1 displayed growth inhibitory activity against CNE-2Z cells with ICvalue of 16.7μM for 72 *** analysis of TBMS1 uptake by CNE-2Z cells displayed the initial slow TBMS1 uptake and then gradually reaching an maximum uptake near 18 ***-2Z cells treated with TBMSI(25μM,3 h)were sufficient to cause the microtubular network disruption,lmmunohlot analysis showed that the proportion of cytosolic tubulin of cells treated with TBMS1 increased in a time-and concentration-dependent ***1 did not inhibit the binding of vinblastine to *** binding to tubulin was inhibited in the presence of ***:TBMS1 is an anti-microtubule agent,and its binding site of tubulin is the colchicine binding site of tubulin.

关键词： Bolbostemma paniculatum(Maxim.)Franquet Tubeimoside I Uptake kinetics Anti-microtubule activity Colchicine binding site of tubulin

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Activation of KCNQ potassium channels by small chemical moleculars

Activation of KCNQ potassium channels by small chemical mole...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Zhaobing GAO~1,Min LI~(1,2) 1 State Key laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,555 Zu Chong Zhi Road,Zhangjiang Hi-Tech Park,Shanghai 201203,China 2 Department of Neuroscience,High Throughput Biology Center and Johns Hopkins Ion Channel Center,school of medicine,Johns Hopkins university,baltimore,maryland 21205

＜正＞KCNQ potassium channels play a critical role in controlling membrane *** a typical voltagegated ion channels（Kv）,KCNQ channels undergo closed to open transitions by sensing transmembrane potential changes and mediate inhibitory potassium *** of KCNQ channel activity as results of genetic mutation is causal to a variety of human diseases including epilepsy,arrhythmia and ***,discovery of small compounds to activate voltage-gated ion channels is an important strategy for clinical intervention in human diseases caused by membrane hyperexcitability,including anxiety,epilepsy,arrhythmia and neuropathic *** the binding of these molecules induces a conformational change leading to the opening of these channels,there is also a considerable conceptual interest in understanding the molecular basis of these "gain of a function" *** molecule activators of cation channels are rare;however,several novel compounds have been identified for KCNQ potassium ***,we reported two new molecules which potentiate either cardical or neuronal KCNQ channels and identified molecular determinants for their activity,which provide exciting insights into activation of voltage-gated channels by small molecuars.

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From Biology to Targeted Cancer Treatment and Prevention

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中国肺癌杂志 2009年第6期 62-62页

作者： Li MAO Professor and Chairman, Department of Oncology and Diagnostic Sciences, university of maryland Dental school, maryland, usa

Cancer is a complex disease involving accumulation of multiple molecular abnormalities during a long period of time. Because the differences in timing and types of abnormalities, together with differences in inherited... 详细信息

关键词： From Biology to Targeted Cancer Treatment and Prevention

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