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chinese national clinical practice guideline on diagnosis and treatment of biliary tract cancers

中华医学杂志(英文版) 2024年第19期137卷 2272-2293页

作者： Wang Xu’an Bai Yongrui Chai Ningli Li Yexiong Linghu Enqiang Wang Liwei Liu Yingbin Society of Hepato-pancreatobiliary Surgery of chinese Research Hospital Association Society of Digestive Endoscopy of the chinese medical Association chinese medical Journal Clinical Practice Guideline Collaborative Department of Biliary and Pancreatic Surgery Renji Hospital Shanghai Jiao Tong University School of Medicine State Key laboratory of Systems Medicine for Cancers Shanghai Cancer Institute Shanghai Key laboratory for Cancer Systems Regulation and Clinical Translation Shanghai China Department of Radiation Oncology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China Department of Gastroenterology and Hepatology the First Medical Center Chinese People’s Liberation Army General Hospital Beijing China State Key laboratory of molecular Oncology and Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC) Beijing China State Key laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Department of Oncology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China

Background: Biliary tract carcinoma (BTC) is relatively rare and comprises a spectrum of invasive tumors arising from the biliary tree. The prognosis is extremely poor. The incidence of BTC is relatively high in Asian countries, and a high number of cases are diagnosed annually in china owing to the large population. Therefore, it is necessary to clarify the epidemiology and high-risk factors for BTC in china. The signs associated with BTC are complex, often require collaborative treatment from surgeons, endoscopists, oncologists, and radiation therapists. Thus, it is necessary to develop a comprehensive chinese guideline for ***: This clinical practice guideline (CPG) was developed following the process recommended by the World Health Organization. The Grading of Recommendations Assessment, Development, and Evaluation approach was used to assess the certainty of evidence and make recommendations. The full CPG report was reviewed by external guideline methodologists and clinicians with no direct involvement in the development of this CPG. Two guideline reporting checklists have been adhered to: Appraisal of Guidelines for Research and Evaluation (AGREE) and Reporting Items for practice Guidelines in Healthcare (RIGHT).Results: The guideline development group, which comprised 85 multidisciplinary clinical experts across china. After a controversies conference, 17 clinical questions concerning the prevention, diagnosis, and treatment of BTC were proposed. Additionally, detailed descriptions of the surgical principles, perioperative management, chemotherapy, immunotherapy, targeted therapy, radiotherapy, and endoscopic management were ***: The guideline development group created a comprehensive chinese guideline for the diagnosis and treatment of BTC, covering various aspects of epidemiology, diagnosis, and treatment. The 17 clinical questions have important reference value for the management of BTC.

关键词： Biliary tract carcinoma Etiology Diagnosis Treatment Surgery

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Antibody gene therapy: an attractive approach for the treatment of cancers and other chronic diseases

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Cell Research 2007年第4期17卷 303-306页

作者： Dexian Zheng ~1national laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences and peking union medical college,beijing 100005,china national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese A cademy of Medical Sciences and Peking Union Medical College Beijing 100005 China

Monoclonal antibodies(mAb)have been successfullyapplied to the treatment of chronic diseases,such as cancer,inflammation and immune *** the technicaladvances in antibody engineering,development of smallrecombinant antibody fragments as high affinity therapeu-tics with reduced immunogenicity has become under thespotlight.A popular format of the engineered recombinantantibody fragments is the single-chain fixed-variable(scFv)molecules,in which the VH and VL regions of the parentalantibody are jointed together by a polypeptide *** fragment retains the target specificity and antigen-binding affinity of the intact antibody,and can be geneti-cally designed and produced in large quantity by ectopicallyexpressing both VH and VL regions from a single cDNAin *** to its smaller size,the scFv molecule showsimproved pharmacokinetics in tumor penetration and isbetter tolerated by the host immune *** thesepotential advantages of using scFv molecules for immu-notherapy,especially for chronic diseases such as cancers,the treatment efficacy,however,is often compromised bythe rapid blood clearance and insufficient concentration ofthe infused scFv fragments at the target *** achievehigh local concentrations in the target tissues,large amountof the recombinant scFv proteins(hundreds milligrams or

关键词： gene an attractive approach for the treatment of cancers and other chronic diseases Antibody gene therapy

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Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis

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Cell Research 2003年第3期13卷 171-177页

作者： SHENG WEI ZOU,JIAN CHAO ZHANG,XIAO DONG ZHANG,SHI YING MIAO,SHU DONG ZONG,QI SHENG,LIN FANG WANGnational laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences,peking union medical college,5 Dong Dan 3-tiao,beijing 100005,chinanational B,esearch institute for Family Planing,WHO Collaboration Center for B,esearch in Human Reproduction,12 Da Hui Si,beijing 100081,china 1national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 2national Research institute for Family Planing WHO Collaboration Center for Research in Human Reproduction 12 Da Hui Si Beijing 100081 China 1national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 1national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 2national Research institute for Family Planing WHO Collaboration Center for Research in Human Reproduction 12 Da Hui Si Beijing 100081 China 1national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China 1national laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College 5 Dong Dan 3-tiao Beijing 100005 China

Variable Charge X/Y(VCX/Y)is human testis-specific gene family that localized on X and Y *** this study,VCY protein was expressed in *** in the form of glutathione-S-transferase(GST)fusion *** the purified fusion protein as antigen,the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by *** the testisseminiferous epithelium,VCY proteins were highly expressed in nuclei of germ *** propidium io-dide staining and green fluorescent protein(GFP)tag technologies,VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 *** *** colocalization for VCY and VCX-8r in COS7 cellswas also *** VCY cDNA as bait,a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid *** the information above indicated that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis.

关键词： VCX/Y protein spermatogenesis nucleoli colocnlization.

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Comparison of differential gene expression profiles in human esophageal squamous carcinoma EC8712 cells before and after arsenic trioxide (As_2O_3) treatment

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chinese science Bulletin 1999年第17期44卷 1581-1587页

作者： XIE Dongxu, DING Fang, WANG Xiuqin, LIU Zhihua, LUO Aiping and WU Minnational laboratory of molecular Oncology, Department of Cell biology, Cancer institute , chinese academy of medical sciences and peking union medical college , beijing 100021, china national laboratory of molecular Oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

To elucidate molecular mechanisms of As2O3 induced apoptosis of cancer cells in vitro, Atlas human cDNA expression analysis has been used for the profile of the known genes expressed in the human esophageal squamous carcinoma cells before and after treated by As2O3. On treating EC8712 cells with As2O3, most of the oncogenes have been down-regulated, while some tumor suppressor genes, such as DCC, up-regulated. Cyclin H decreases,

关键词： arsenic trioxide esophageal squamous carcinoma cell apoptosis gene expression.

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Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice

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Biomedical and Environmental sciences 2003年第4期16卷 323-332页

作者： YUE-XIN LIANG, ZHEN WANG, DIAN-DONG LI, JIAN-MIN JIANG,AND RONG-GUANG SHAOinstitute of Medicinal Biotechnology, chinese academy of medical sciences and peking union medical college, beijing 100050, china institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 China

Objective To analyze the effects of aging or advanced glycation on gene expression in the cerebrum and spleen of female C57BL/6J mice. Methods The gene expression profile was determined by using cDNA expression arrays containing 588 cDNA. Results Aging and advanced glycation resulted in differential gene expression patterns of cerebrum and spleen compared with young mice. Among the 80 genes detected in cerebrum, 43 exhibited a change in mRNA ratios with aging or treatment. Thirty-four changes (79%) were common in aged and D-galactose treated mice, whereas the cerebrum from aged and AGE-lysine treated mice showed common changes in expression of 38 genes (88%). Of the 86 genes detected in spleen, 29 (34%) displayed an age-related decrease in expression, whereas 3 (3%) displayed an increase in expression levels with aging. Eighteen genes from the detectable genes exhibited expression changes in both cerebrum and spleen of mice. Conclusions The gene expression profiles of D-galactose and AGE-lysine treated mice resemble those of aged mice. Use of cDNA hybridization arrays may provide a promising tool to explore the mechanism of aging at a molecular level.

关键词： Aging Galactose Advanced glycation Gene expression DNA microarrays Cerebrum Spleen

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在Yunnanese（Aγδβ）^0—地贫3′缺失端点下游鉴别到两个增强子样顺序

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生物化学与生物物理进展 2002年第1期29卷 114-119页

作者：黄小东张俊武中国医学科学院中国协和医科大学基础医学研究所

利用荧光酶报告基因系统搜索了Yunnanese (Aγδβ) 0 地中海贫血缺失 3′端点下游 11 5kb区域内的调控顺序 .确定缺失 3′端点立即下游区 1 7kb片段 ,在人红白血病细胞K5 6 2及鼠红白血病细胞MELGM979中 ,可使γ 珠蛋白基因启动子驱... 详细信息

利用荧光酶报告基因系统搜索了Yunnanese (Aγδβ) 0 地中海贫血缺失 3′端点下游 11 5kb区域内的调控顺序 .确定缺失 3′端点立即下游区 1 7kb片段 ,在人红白血病细胞K5 6 2及鼠红白血病细胞MELGM979中 ,可使γ 珠蛋白基因启动子驱动的荧光酶基因表达增加 3 8～ 4 0倍 ,而在HeLa细胞中仅增加 1 5倍 .位于缺失 3′端点约 10kb的一个长 1 4kb片段在K5 6 2和MELGM979中 ,可使γ 基因启动子驱动的荧光酶基因表达增加 2 4～2 9倍 ,而在HeLa细胞中无增加 .结果说明这两段顺序均有增强子活性 ,并且这种活性具有一定的红细胞特异性 .进一步证明 1 7kb片段内包含多个转录调节蛋白结合模体的 430bp片段包含了 1 7kb片段的大部分增强子活性 .这些结果为缺失导致增强子样顺序并入到接近Gγ 基因 ,是Yunnanese (Aγδβ) 0 地贫缺失突变体中胎儿Gγ 珠蛋白基因 ,在成人期持续活跃表达原因的假设提供了实验证据 .

关键词： Gγ-珠蛋白基因 γ(Aδβ)^0-地中海贫血增强子样顺序成人胎儿

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一个可能与T细胞发育相关的新的C2H2型锌指蛋白基因的克隆(英文)

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生物化学与生物物理进展 2004年第7期31卷 600-605页

作者：杜占文张昕张俊武中国医学科学院中国协和医科大学基础医学研究所

一些在组织和细胞分化中起重要作用的蛋白质包含锌指结构域 .为了克隆分离和研究与造血细胞分化和发育成熟相关的蛋白基因 ,利用编码C2H2型锌指蛋白结构域中部分保守氨基酸序列设计简并引物 ,以骨髓cDNA为模板 ,进行PCR扩增 ,得到若干... 详细信息

一些在组织和细胞分化中起重要作用的蛋白质包含锌指结构域 .为了克隆分离和研究与造血细胞分化和发育成熟相关的蛋白基因 ,利用编码C2H2型锌指蛋白结构域中部分保守氨基酸序列设计简并引物 ,以骨髓cDNA为模板 ,进行PCR扩增 ,得到若干新的锌指蛋白基因EST .用其中一条为探针筛选人骨髓cDNA文库 ,获得了一个新的锌指蛋白基因全长cDNA ,GenBank收录号为AF2 4 6 12 6 ,长 3888bp ,包括一个完整阅读框 ,编码 6 86个氨基酸 ,包括 17个典型的和 2个非典型的C2H2模体 ,命名为HZF2 .RNA印迹、人多组织mRNA斑点杂交分析结果显示 ,其在T淋巴细胞发育和定居的器官组织胸腺、淋巴结中有较高表达 ,在脾脏、胎肝有中度表达 ,在B淋巴细胞发育的骨髓中表达很低 ,在外周血几个淋巴细胞系中仅有极微量的表达 ,提示HZF2可能对于T淋巴细胞发育和增殖有重要功能 .该基因也在脑组织的若干部位、胎盘及肾上腺有较高表达 ,在多种其他组织细胞有微量表达 ,说明其可能对维持这些组织细胞的生理功能也起一定作用 .将编码HZF2读框的DNA顺序克隆到pEGFP N1载体中 ,转染 3T3细胞 ,证明表达的HZF2 GFP融合蛋白定位于细胞核 ,这与根据HZF2蛋白结构推测其可能作为DNA结合蛋白行使调节基因转录的功能是一致的 .

关键词：锌指蛋白基因克隆 cDNA文库筛选表达谱 T细胞发育

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Knockdown of Human p53 Gene Expression in 293-T Cells by Retroviral Vector-mediated Short Hairpin RNA

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 779-783页

作者： De-Long HAO Chang-Mei LIU Wen-Ji DONG Huan GONG Xue-Song WU De-Pei LIU Chih-Chuan LIANG national laboratory of medical molecular biology,institute of basic medical sciences,chinese academy of medical sciences and peking union medical college,beijing 100005,china nationallaboratoryofmedicalmolecularbiology InstituteofBasicMedicalSciencesChineseAcademyofMedicalSciences

RNA interference(RNAi)is an evolutionarily conserved process of gene silencing in multipleorganisms,which has become a powerful tool for investigating gene function by reverse ***,many groups have reported to use synthesized oligonucleotides or siRNA encoding plasmids to induce RNAiin mammalian cells by transfection,but this is still limited in its application,especially when it is necessary togenerate long-term gene silencing in *** circumvent this problem,retrovirus- or lentivirus-deliveredRNAi has been ***,we described two retroviral systems for delivering short hairpin RNA(shRNA)transcribed from the H1 *** results showed that retroviral vector-mediated RNAi cansubstantially downregulate the expression of human p53 in 293-T ***,the retroviral vector-mediated RNAi in our transduction system can stably inactivate the p53 gene for a long *** toshRNAs transcribed from the U6 promoter,H1-driven shRNA also dramatically reduced the expression *** p53 downregulation efficiencies of H1-and U6-driven shRNAs were almost *** resultsindicate that retroviral vector-delivered RNAi would be a useful tool in functional genomics and gene therapy.

关键词： retroviral vector RNA interference p53

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一种抑制pGEX载体系统本底表达的方法

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生物化学与生物物理进展 1998年第1期25卷 87-89页

作者：黄蓬缪时英王琳芳中国医学科学院基础医学研究所中国协和医科大学基础

用ｐＧＥＸ载体系统体外构建了一种人精子膜蛋白片段（ＨＳＤⅡ）的重组表达质粒．未经ＩＰＴＧ诱导，该质粒表达的融合蛋白在ＤＨ５α中即有较高的本底表达量．若将带有ＬａｃⅠ基因的ｐＲＥＰ４质粒与重组表达质粒共转化ＤＨ５α菌，... 详细信息

用ｐＧＥＸ载体系统体外构建了一种人精子膜蛋白片段（ＨＳＤⅡ）的重组表达质粒．未经ＩＰＴＧ诱导，该质粒表达的融合蛋白在ＤＨ５α中即有较高的本底表达量．若将带有ＬａｃⅠ基因的ｐＲＥＰ４质粒与重组表达质粒共转化ＤＨ５α菌，则可有效抑制融合蛋白的本底表达．

关键词： pGEX 载体系统精子膜蛋白重组表达质粒

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Induction, modulation and potential targets of miR-210 in pancreatic cancer cells

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Hepatobiliary & Pancreatic Diseases International 2012年第3期11卷 319-324页

作者： Wei-Yun Chen, Wen-Jing Liu,Yu-Pei Zhao, Li Zhou, Tai-Ping Zhang, Ge Chen and Hong Shu Department of General Surgery, peking union medical college Hospital, chinese academy of medical sciences/peking union medical college, beijing 100730, china Department of General Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences/Peking Union Medical College Beijing 100730 China

BACKGROUND: MiR-210 is induced by hypoxia and plays different roles in the development of certain cancers. However, little is known about its role in pancreatic cancer (PC). This study aimed to explore the induction and modulation of PC by miR-210 and its potential molecular targets. METHODS: PC cells were cultured under normoxic and hypoxic conditions. Expression of miR-210 and hypoxia-inducible factor (HIF)-1α was detected using quantitative reverse-transcription polymerase chain reaction. Cancer cells were transiently transfected with HIF-1α small interfering RNA (siRNA) and miR-210 mimics, and cell proliferation was measured using the CCK-8 assay. Potential targets for miR-210 were then identified using a dual luciferase reporter assay. RESULTS: Hypoxic conditions induced miR-210 expression in six PC cell lines (AsPC-1, BxPC-3, MIAPaCa-2, PANC-1, Su86.86 and SW1990), but not in Capan-1 or T3M4 cells. Transfection of HIF-1α siRNA into PANC-1 cells markedly inhibited HIF-1α expression, and subsequently down- regulated miR-210 expression under hypoxic conditions. MiR-210 had no observable impact on the proliferation of PANC-1 or Su86.86 cells and dual luciferase reporter assays showed significantly reduced luciferase activity in the wild- type E2F3, EFNA3, GIT2, MNT, ZNF462 and EGR3 constructs, compared to the corresponding mutants, but not in HOXA3. CONCLUSIONS: These results suggest that miR-210 expression in PC cells is induced by hypoxia through a HIF-1α-dependent pathway, but does not influence PC cell proliferation. Also, E2F3, EFNA3, GIT2, MNT, ZNF462 and EGR3 may be potential miR-210 targets in PC.

关键词： miR-210 hypoxia HIF-1α pancreatic cancer proliferation

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