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上海市松江区居民吸烟状况分析

上海预防医学 2002年第12期14卷 580-582页

作者：邱桦傅东波傅华朱美英陈平复旦大学公共卫生学院上海200032 上海市松江区疾病预防控制中心上海201600

[目的]了解上海市松江区居民的吸烟情况。[方法]在松江地区4个镇1个街道共16万人口中随机选择4772名居民采用统一标准的调查问卷进行入户调查。[结果] 被调查人群中现在吸烟率为23.62％,曾经吸烟率为2.83％,居民现在吸烟率与年龄、性别... 详细信息

[目的]了解上海市松江区居民的吸烟情况。[方法]在松江地区4个镇1个街道共16万人口中随机选择4772名居民采用统一标准的调查问卷进行入户调查。[结果] 被调查人群中现在吸烟率为23.62％,曾经吸烟率为2.83％,居民现在吸烟率与年龄、性别和文化程度有关P值均43岁,最主要的原因是好奇、社交需要和解乏。77.3％的现在吸烟者还没有考虑在未来6个月内戒烟。被动吸烟率为65.13％,被动吸烟的场所主要是家中和公共场所。[结论] 松江地区居民的吸烟率低于全国1996年调查的15岁以上人群吸烟率(35.3％)。但松江地区大多数吸烟者尚未考虑戒烟,需要开展有针对性的控烟干预。大多数非吸烟者都受到被动吸烟的危害,有必要采取措施减少在家中和公共场所等地方被动吸烟的机会。

关键词：上海松江区吸烟被动吸烟居民卫生调查

A novel PLA2G6 gene mutation causes autosomal recessive early-onset parkinsonism with typical clinical phenotype of Parkinson’s disease

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A novel PLA2G6 gene mutation causes autosomal recessive earl...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Ji-Feng GUO~(1,3),Chang-He SHI~1,Lei WANG~1,Zhan-Yun LV~1,Jian WANG~4,Lin-Zi LUO~1,Lu SHEN~(1,3),Hong JIANG~(1,3),Xin-Xiang YAN~(1,3),Qian PAN~2,Kun XIA~2,Bei-Sha TANG~(1,2,3,*) 1 Department of Neurology,Xiangya Hospital,Central South University,Changsha 410008,china 2 national laboratory of Medical Genetics of china,Changsha 410008,china 3 Neurodegenerative Disorders Research center,Central South University,Changsha 410008,china 4 Department of Neurology,Huashan Hospital,Fudan University,shanghai 200040,china

＜正＞Objective Mutations in the PLA2G6 gene at the PARK14 locus have been reported in complicated parkinsonism *** assess the prevalence of and phenotypes associated with PLA2G6 gene mutations,we screened PLA2G6 mutations in a cohort of patients with autosomal recessive early-onset parkinsonism（AREP）. Methods We selected 12 families with AREP in which the Parkin,PINK1,DJ-1,ATP13A2,and FBX07 gene mutations had been previously *** patients came from the mainland of *** entire PLA2G6 coding region and exon-intron boundaries were sequenced from genomic DNA *** then performed PET studies on individuals in the pedigree with a homozygous PLA2G6 mutation,and investigated the enzyme activity level of the *** A homozygous missense mutation,C.G991T（p.D331Y） was identified in an autosomal recessive case.A younger sister of the p.D331Y-carrying patient was also homozygous for the mutation,but was with no extrapyramidal symptoms.A PET study showed a substantial reduction in dopamine transporter（DAT） binding in the p.D331Y patient,and a slight reduction in DAT binding in his *** vitro,we experimentally demonstrate that the D331Y mutation caused an approximately 70%reduction in enzyme *** We have confirmed that the PLA2G6 gene allocated PARK14 locus and is associated with AREP.

关键词： PLA2G6 PARK14 PET AREP

Analysis on the Quality of Cosmetics Tested in Xiamen City during 2005 to 2009

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Analysis on the Quality of Cosmetics Tested in Xiamen City d...

2010 the 5th International Academic Conference on Environmental and Occupational Medicine

作者： HUANG Xiao-jin,WANG Bao-zhen,BAI Hong Xiamen center for disease Control and Prevention,Xiamen 361021,Fujian,china

[Objective]To provide evidence-based advice for supervision and regulation by analyzing the status quo and trends of cosmetic quality in Xiamen from 2005 to 2009.[Methods]In accordance with the"Hygiene Regulation for Cosmetics"by Ministry of health,820 cosmetic samples in Xiamen were tested and analyzed for the indicators such as concentrations of Pb,As and Hg,total bacteria count,and number of Staphylococus aureus,Pseudomonas aerugiouosa,fungi and *** strains in feces.[Results]The qualification rate of cosmetics increased from 88.41%in 2005 to 100%(χ=14.35,P4000mg/kg,whieh was tens of thousand times higher that the national limits.[Conclusion]The priority of inspection should be transferred to beauty *** it is necessary to strengthen daily surveillance and spot check,and enhance the training of practitioners.

关键词： cosmetic hygiene quality testing

T载体的构建及日本血吸虫肌动蛋白基因PCR产物的快速克隆

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中国血吸虫病防治杂志 2004年第4期16卷 277-280页

作者：沈玉娟曹建平刘述先徐馀信宋光承中国疾病预防控制中心寄生虫病预防控制所

目的　构建快速高效克隆 PCR产物的克隆载体 (T载体 ) ,并对日本血吸虫肌动蛋白全长编码基因 PCR产物进行快速克隆。方法　日本血吸虫肌蛋白全长编码基因的扩增采用逆转录 -聚合酶链反应 (RT- PCR)方法。质粒 p GEM5 Zf (+)经限制性内切... 详细信息

目的　构建快速高效克隆 PCR产物的克隆载体 (T载体 ) ,并对日本血吸虫肌动蛋白全长编码基因 PCR产物进行快速克隆。方法　日本血吸虫肌蛋白全长编码基因的扩增采用逆转录 -聚合酶链反应 (RT- PCR)方法。质粒 p GEM5 Zf (+)经限制性内切酶 Eco R V酶切 ,在仅含有脱氧胸苷三磷酸 (d TTP)的 PCR缓冲液中于 70℃作用 2 h,在每个片段的 3′端加上一个脱氧胸苷 (d T)碱基 ,构建成 T载体。根据 PCR扩增产物 3′端存在一个非模板依赖的脱氧腺苷 (d A)原理 ,将扩增产物直接克隆入 T载体并测序。结果　阳性克隆经琼脂糖凝胶电泳、限制性酶切分析、PCR及 DNA序列测定等均证实克隆获得成功 ,且效率很高。与曼氏血吸虫肌动蛋白比较 ,核苷酸和推断的氨基酸的同源性分别是 92 .5 %和 99.7%。结论　构建的 p GEM5 Zf- T载体对日本血吸虫肌动蛋白编码基因的PCR产物直接克隆既经济、简便 ,又快速、高效 ,所构建的 T载体由于在插入位点两侧具有 p UC/M13测序引物序列 ,可直接测定重组质粒中插入片段的核苷酸序列。所获得的日本血吸虫 (大陆株 )

关键词：日本血吸虫肌动蛋白 PCR T载体测序

乙酰甲胆碱(氯醋甲胆碱)支气管激发试验技术规范(2023年版)

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中华结核和呼吸杂志 2024年第2期47卷 101-119页

作者：中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会中华医学会呼吸病学分会中国老年医学会呼吸分会肺功能学组郑劲平宋元林高怡黄刚李丽梁斌苗逯勇谢燕清赵桂华不详国家呼吸医学中心、国家呼吸疾病临床医学研究中心、呼吸疾病全国重点实验室、广州呼吸健康研究院、广州医科大学附属第一医院呼吸与危重症医学科广州510120 复旦大学附属中山医院呼吸与危重症医学科上海200032 广州医科大学附属第一医院浙江大学医学院附属第二医院复旦大学附属中山医院四川大学华西医院首都医科大学附属北京朝阳医院河南省人民医院

乙酰甲胆碱支气管激发试验(MCT)是评估气道高反应性及其程度的常用检查,对支气管哮喘诊断及治疗效果评估具有重要临床价值。中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会、中华医学会呼吸病学分会肺功能学组(筹)和中国老年... 详细信息

乙酰甲胆碱支气管激发试验(MCT)是评估气道高反应性及其程度的常用检查,对支气管哮喘诊断及治疗效果评估具有重要临床价值。中国医师协会呼吸医师分会肺功能与临床呼吸生理工作委员会、中华医学会呼吸病学分会肺功能学组(筹)和中国老年医学会呼吸分会肺功能学组的专家们共同组成共识工作组,基于2014年发布的《肺功能指南——支气管激发试验检查》及其在使用中存在的问题与近年研究进展,在充分收集专家组意见、查阅文献、问卷调研、多轮线上和线下讨论后,形成了《乙酰甲胆碱(氯醋甲胆碱)支气管激发试验技术规范(2023年版)》。本共识收集了国内MCT临床实践中核心的11个问题并形成推荐意见,内容包括MCT的适应证与禁忌证、激发试剂配制、试验流程与方法、质量控制、安全性管理、结果解读和报告规范等方面,为医疗机构临床肺功能从业人员开展MCT提供参考,以期更好应用该项技术指导临床诊疗。

关键词：支气管激发试验安全性管理中国医师协会中华医学会推荐意见乙酰甲胆碱问卷调研气道高反应性

Neural mechanism of electroacupuncture analgesia on chronic inflammatory pain

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Neural mechanism of electroacupuncture analgesia on chronic ...

2011中国针灸学会年会

作者： Wen-Li Mi,Gen-Cheng Wu*,Yan-Qing Wang* Department of Integrative Medicine and Neurobiology,Institute of Acupuncture Research(WHO Collaborating center for Traditional Medicine),Fudan University,shanghai 200032,P.R.china

Background Arthritis is a painful and disabling disease that affects millions of ***,an important part of Traditional chinese Medicine(TCM),has been confirmed effective in pain relief by numerous clinical observations...

关键词：

Responsible genes in children with primary vesicoureteral reflux: findings from the chinese Children Genetic Kidney disease Database

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World Journal of Pediatrics 2021年第4期17卷 409-418页

作者： Jia-Lu Liu Qian Shen Ming-Yan Wu Guang-Hua Zhu Yu-Feng Li Xiao-Wen Wang Xiao-Shan Tang Yun-Li Bi Yi-Nv Gong Jing Chen Xiao-Yan Fang Yi-Hui Zhai Bing-Bing Wu Guo-Min Li Yu Bo Sun Xiao-Jie Gao Cui-Hua Liu Xiao-Yun Jiang Sheng Hao Yu-Lin Kang Ying-Liang Gong Li-Ping Rong Di Li Si Wang Duan Ma Jia Rao Hong Xu chinese Children Genetic Kidney disease Database(CCGKDD) “Internet Plus”Nephrology Alliance of the national center for Children’s Care Department of Nephrology Children’s Hospital of Fudan UniversityNational Children’s Medical Center399 Wan Yuan RoadShanghai201102People’s Republic of China shanghai Kidney Development and Pediatric Kidney disease Research center ShanghaiChina Department of Nephrology Shanghai Children’s HospitalShanghaiChina Department of Pediatric Nephrology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiChina Wuhan Children’s Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Urology Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Department of Rheumatology Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Clinical Genetic center Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Department of Nephrology Shenzhen Children’s HospitalShenzhenGuangdongChina Department of Nephrology and Rheumatology Children’s Hospital Affiliated to Zhengzhou UniversityZhengzhou Key Laboratory of Pediatric Kidney Disease ResearchZhengzhouChina The First Affiliated Hospital of Zhongshan University GuangzhouChina Key laboratory of Metabolism and Molecular Medicine Ministry of EducationDepartment of Biochemistry and Molecular BiologyInstitutes of Biomedical SciencesSchool of Basic Medical SciencesFudan UniversityShanghaiChina 不详

Background Primary vesicoureteral reflux(VUR)is a common congenital anomaly of the kidney and urinary tract(CAKUT)in *** present study identified the possible genetic contributions to primary VUR in *** Patients with primary VUR were enrolled and analysed based on a national multi-center registration network(chinese Children Genetic Kidney disease Database,CCGKDD)that covered 23 different provinces/regions in china from 2014 to *** causes were sought using whole-exome sequencing(WES)or targeted-exome *** A total of 379 unrelated patients(male:female 219:160)with primary VUR were ***-four(16.9%)children had extrarenal manifestations,and 165(43.5%)patients showed the coexistence of other CAKUT ***-eight patient(23.2%)exhibited impaired renal function at their last visit,and 18 of them(20.5%)developed ESRD at the median age of 7.0(IQR 0.9–11.4)years.A monogenic cause was identified in 28 patients(7.39%).These genes included PAX2(n=4),TNXB(n=3),GATA3(n=3),SLIT2(n=3),ROBO2(n=2),TBX18(n=2),and the other 11 genes(one gene for each patient).There was a significant difference in the rate of gene mutations between patients with or without extrarenal complications(14.1%vs.6%,P=0.035).The frequency of genetic abnormality was not statistically significant based on the coexistence of another CAKUT(9.6%vs.5.6%,P=0.139,Chi-square test)and the grade of reflux(9.4%vs.6.7%,P=0.429).Kaplan–Meier survival curve showed that the presence of genetic mutations did affect renal survival(Log-rank test,P=0.01).PAX2 mutation carriers(HR 5.1,95%CI 1.3–20.0;P=0.02)and TNXB mutation carriers(HR 20.3,95%CI 2.4–168.7;P=0.01)were associated with increased risk of progression to *** PAX2,TNXB,GATA3 and SLIT2 were the main underlying monogenic causes and accounted for up to 46.4%of monogenic *** complications and renal function were significantly related to the findings of genetic factors in children with p

关键词： Children Congenital anomalies of the kidney and urinary tract Gene mutation Vesicoureteral reflux Whole-exome sequencing

Ⅰ型神经纤维瘤病临床诊疗专家共识（2021版）

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中国修复重建外科杂志 2021年第11期35卷 1384-1395页

作者：王智超李青峰中国Ⅰ型神经纤维瘤病多中心治疗协作组全国整形外科多中心研究平台上海交通大学医学院附属第九人民医院整复外科

I型神经纤维瘤病(neurofibromatosis type 1,NF1)是NF1基因突变引起的常染色体显性遗传性肿瘤性疾病,是目前医学诊疗的重点和难点之一。患者病变以神经纤维瘤为特征性表型,其中皮肤型神经纤维瘤数量大,丛状神经纤维瘤累及主干神经,恶变... 详细信息

I型神经纤维瘤病(neurofibromatosis type 1,NF1)是NF1基因突变引起的常染色体显性遗传性肿瘤性疾病,是目前医学诊疗的重点和难点之一。患者病变以神经纤维瘤为特征性表型,其中皮肤型神经纤维瘤数量大,丛状神经纤维瘤累及主干神经,恶变后的恶性外周神经鞘瘤生存期极短。同时NF1患者伴有多系统累及,疾病致畸率、致残率高,手术难以完整切除,治疗难度大。目前中国尚缺乏对于NF1的诊疗共识,不同学科对NF1的疾病认识不同,诊疗存在差异。多学科系统评估、合作诊疗是提高NF1患者治疗水平、生存质量及改善疾病预后等的关键。2020年由上海交通大学医学院附属第九人民医院整复外科牵头成立了中国首个NF1多中心治疗协作组,并进一步联合国内肿瘤外科、肿瘤内科、皮肤科、生殖医学科等学科的知名专家,共同撰写了《Ⅰ型神经纤维瘤病临床诊疗专家共识(2021版)》,旨在推进覆盖NF1患者全生命周期的规范化、同质化诊疗,提高中国NF1的诊疗水平和治疗效果。

关键词： Ⅰ型神经纤维瘤病专家共识临床诊疗中国Ⅰ型神经纤维瘤病多中心治疗协作组

Cross-subtype neutralizing antibodies in treatment-naive HI V-1 -infected individuals in china and characteristics of viral envelope derived from broad neutralizes

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Cross-subtype neutralizing antibodies in treatment-naive HI ...

2012全国临床微生物与感染免疫学术研讨会

作者： Ling Hong~1,Zhong Ping~3,Ren Caiyun~1,Liu Song~1,Li Yan~1,Yu Haotong~1,Zhuang Min~1,Wei Guochao~1, Wang Jiaye~1,Chen Zhijie~3,Sun Feng~3,Liu Wei~4,Liang Shujia~4 1.Harbin Medical University,Heilongjiang province 2.shanghai Municipal center for disease Control and Prevention,Shangha 3.Yili Prefecture CDC,Xinjinag province 4.Guangxi CDC,Guangxi province

Background Little is known about breadth of neutralizing antibody(NAb) responses and the viral envelope characterization in chinese treatment-naive individuals. Objectives To clarify the profile of NAb from broad HIV-1 neutralizers and to find potential immunogen. Methods 866 plasma samples from antiretroviral treatment-naive HIV-1-infected individuals,who mostly had acquired infection over 3 years through injecting drug use(IDU) and sexual contact,living in Xinjiang province (617) and Guangxi province(249) were analyzed for their ability to neutralize diverse pseudoviruses using the TZM-b1 pseudovirus *** NAb breadth was evaluated based on both number of viruses neutralized and neutralization *** genome amplification(SGA) of envelope genes and sequencing were performed to identify the sequence characteristics of the envelope glycoproteins from broad neutralizers. Results We have found that in Xingjiang province,where most samples were derived from IDUs,20 of 617 (3.2%) plasma neutralized all of four subtype B and 39(5.3%) neutralized all of four subtype C at virus inhibition>80%.Only 10(1.6%) and 5(0.8%) plasma were able to neutralize the viruses of both subtype B and C.5 (0.8%) plasma were able to neutralize all 11 viruses of three different subtypes B,C,and CRF01E at virus inhibition>80%.However,in Guangxi province where most samples were derived from sexual contact infection, lower neutralization titers were found with the same virus *** variations of viral envelope glycoproteins between broad and non-broad neutralizers showed some unique characteristics. Conclusions The breadth and potency of Nab against diverse HIV-1 subtypes in chinese HIV-1-infected individuals was clarified for the first time.

关键词： HIV-1 Neutralization antibody Envelope glycoprotein Broad neutralizers