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胰岛素、胰高血糖素和地塞米松调节PEPCK启动子活性的体外研究

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复旦学报（医学版） 2003年第4期30卷 338-341页

作者：沈坤堂秦新裕张新韩泽广复旦大学附属中山医院普外科上海200032 国家人类基因组南方中心功能组上海201203

目的探讨胰岛素、胰高血糖素和地塞米松对PEPCK启动子活性的影响及其意义。方法将PEPCK启动子构建入表达载体pGL3-Basic,采用Lipofect脂质体转染法将携带PEPCK启动子的质粒pGL3-Basic-PEPCK转染至大鼠肝细胞株Hepal-6细胞,12.h后加入... 详细信息

目的探讨胰岛素、胰高血糖素和地塞米松对PEPCK启动子活性的影响及其意义。方法将PEPCK启动子构建入表达载体pGL3-Basic,采用Lipofect脂质体转染法将携带PEPCK启动子的质粒pGL3-Basic-PEPCK转染至大鼠肝细胞株Hepal-6细胞,12.h后加入不同浓度的胰岛素、胰高血糖素和地塞米松,孵育48 h后分别检测其报告基因萤虫素酶的活性。结果生理水平的胰岛素可明显抑制PEPCK启动子的活性,与胰岛素的浓度正相关;地塞米松则上调PEPCK启动子的活性,而胰高血糖素对其活性无影响。结论 PEPCK启动子在肝细胞中受胰岛素的负调控、地塞米松的正调控,若用于调控胰岛素基因的表达,将使糖尿病的基因治疗更安全更可行。

关键词：糖尿病胰岛素胰高血糖素地塞米松 PEPCK启动子基因治疗

A rare polymorphism of the COX7B2.gene in a Cantonese family with nasopharyngeal carcinoma

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Science China(Life Sciences) 2004年第5期47卷 449-453页

作者： LIANG Hui1, CHEN Hankui1, SHEN Yayun2. FENG Qisheng1, JIN Wei2. HUANG Wei2.& ZENG Yixin1 1. Cancer center, Sun Yat-sen University, Guangzhou 510060, China 2. chinese national human genome center at shanghai, {2., China 1. Cancer center Sun Yatsen University 510060 Guangzhou China 2. chinese national human genome center at shanghai 201203 Shanghai China

Nasopharyngeal carcinoma (NPC) is the most common head and neck cancer in southern China, and the genetic susceptibility is believed to play an important role in the aetiol-ogy of this malignancy. In our previous studies, one candidate susceptibility locus has been mapped to chromosome 4p11-p14 in a subset of NPC families. In the present study, we screened the cytochrome oxidase VIIb2.(COX7B2. gene which resides in this region and investigated the relationship of single nucleotide polymorphisms (SNPs) of this gene with these familial NPC pa-tients. We identified five novel SNPs in this gene, among them -158101G>T and -15732.G>A in promoter region, -109602.>G in intron 2. 78T>A in exon 3, and 354T>A in 3?untranslational region. The change 78T>A at codon 2. which leads to CAT2.CAA (His2.Gln) was shared by patients from family 31 that carried the susceptibility haplotype, but not found in cases from other NPC families nor in sporadic cases. However, the frequency of allele A was relatively low in normal controls both from Guangdong and eastern China (0.45% and 0.2.%, respectively), and this variant was not found in pooled DNA samples from the white and the black population. Pro-tein sequence alignment showed that the 2.His of COX7B2.protein is consistent among different species. Our results suggested that the codon 2. of COX7B2.gene might be conservative during the process of evolution, and the rare variation His2.Gln was probably associated with the high risk in NPC pedigree 31.

关键词： COX7B2 gene,nasopharyngeal carcinoma,SNPs.

Jackknife and Bootstrap Tests of the Composition Vector Trees

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genomics, Proteomics & Bioinformatics 2010年第4期8卷 262-267页

作者： Guanghong Zuo1,2. Zhao Xu1,3, Hongjie Yu1,4, and Bailin Hao1,5,6 1T-Life Research center & Department of Physics, Fudan University, shanghai 2.0433, China 2.1} Institute of Applied Physics, chinese Acadamy of Sciences, shanghai 2.1800, China 3Applied Biosystems, Inc., Beijing 10002., China 4Fudan-VARI center for Genetic Epidemiology, Fudan University, shanghai 2.0433, China 5Institute of Theoretical Physics, chinese Acadamy of Sciences, Beijing 100190, China 6Santa Fe Institute, Santa Fe, NM 87505, USA. [a] T-Life Research center & Department of Physics Fudan University shanghai 2.0433 China [b] shanghai Institute of Applied Physics chinese Acadamy of Sciences shanghai 2.1800 China [c] Applied Biosystems Inc. Beijing 10002. China [d] Fudan-VARI center for Genetic Epidemiology Fudan University shanghai 2.0433 China [e] Institute of Theoretical Physics chinese Acadamy of Sciences Beijing 100190 China [f] Santa Fe Institute Santa Fe NM 87505 USA

Composition vector trees （CVTrees） are inferred from whole-genome data by an alignment-free and parameter-free method. The agreement of these trees with the corresponding taxonomy provides an objective justification of the inferred phylogeny. In this work, we show the stability and self-consistency of CVTrees by performing bootstrap and jackknife re-sampling tests adapted to this alignment-free approach. Our ultimate goal is to advocate the viewpoint that time-consuming statistical re-sampling tests can be avoided at all in using this alignment-free approach. Agreement with taxonomy should be taken as a major criterion to estimate prokaryotic phylogenetic trees.

关键词： composition vector phylogeny topological distance bootstrap jackknife CVTree

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中国百日咳诊疗与预防指南(2.2.版)

中华医学杂志 2024年第15期104卷 1258-1279页

作者：中华医学会感染病学分会儿科感染学组国家卫生健康委能力建设和继续教育儿科专委会感染组中国临床实践指南联盟方法学专委会国家儿童医学中心(上海) 国家传染病医学中心曾玫邵祝军张文宏夏君蔡洁皓陈明亮冯天行姚开虎不详复旦大学附属儿科医院感染传染科国家儿童医学中心(上海)上海200032 中国疾病预防控制中心传染病预防控制所北京102206 复旦大学附属华山医院感染科国家传染病医学中心上海200040 宁波诺丁汉大学GRADE中心宁波315100 复旦大学附属儿科医院感染传染科复旦大学附属闵行医院检验科上海临床研究中心儿科首都医科大学附属北京儿童医院北京市儿科研究所感染与微生物研究室国家儿童医学中心(北京)

百日咳再现是全球性的公共卫生问题,2.18—2.2.年我国国内百日咳报告发病率接近2.世纪80年代末的报告水平。实际上,我国的百日咳发病率被严重低估,主要因为国内尚未全面建立百日咳主动监测工作,不典型百日咳病例在临床上容易被漏诊,许... 详细信息

百日咳再现是全球性的公共卫生问题,2.18—2.2.年我国国内百日咳报告发病率接近2.世纪80年代末的报告水平。实际上,我国的百日咳发病率被严重低估,主要因为国内尚未全面建立百日咳主动监测工作,不典型百日咳病例在临床上容易被漏诊,许多医疗机构未开展百日咳的实验室诊断;同时,我国还面临百日咳鲍特菌对一线抗菌药物大环内酯类高度耐药的临床问题。为了更好地指导和规范我国百日咳病例的临床诊疗、监测和防控工作,由感染病学、流行病学、免疫规划及指南方法学专家组成指南制订小组,从实践角度提出有关百日咳诊断、治疗、预防,尤其是疫苗免疫策略的12.临床问题,经过研究问题构建、证据检索和综合、证据评价以及证据到推荐意见讨论,制订推荐意见和实施建议,为从事百日咳诊疗和防控的临床医师、微生物实验室专业人员、医院感染控制专业人员以及公共卫生领域从事传染病防控和免疫规划的专业人员提供参考。

关键词：百日咳诊断治疗预防疫苗接种

Transcriptome profiling analysis reveals multiple modulatory effects of Ginkgo biloba extract in the liver of rats on a high-fat diet

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Transcriptome profiling analysis reveals multiple modulatory...

genome size and sequence composition of moso bamboo: A comparative study

中药药效提高与中药饮片质量控制交流研讨会

作者： Xiaomei Gu~(1,*),Zuoquan Xie~(2.*),Qi Wang~(1,*),Gang Liu~1,Yi Qu~1,Lu Zhang~(1,2.,Jiahu Pan~3,Guoping Zhao~(1,4) and Qinghua Zhang~(1,2.4) 1 national Engineering center for Biochip at {2.,China 2.State Key laboratory of Medical genomics and {2. Institute of Hematology,Ruijin Hospital,{2. Jiaotong University School of Medicine,China 3 School of Pharmacy,Fudan University,{2.,China 4 MOST-{2. Key laboratory of disease and health Related genomics,China

Leaf extract of Ginkgo biloba(GBE) is increasingly used as a herbal medicine for the treatment of neurodegenerative,cardiovascular and cerebrovascular *** studies have demonstrated many protective effects of GBE in neurons,the endothelium and *** this study,we investigated the molecular mechanisms underlying the effects of GBE in disorders induced by long-term exposure to a high-fat diet(HFD).Rats were fed an HFD with or without the GBE product GBE50 for 19 *** found that GBE50 reduced the development of fatty liver induced by an HFD and inhibited the commonly observed elevation of serum cholesterol and lactate dehydrogenase *** profiling analysis showed that several genes were modulated by GBE50 in liver,including those involved in lipid metabolism,carbohydrate metabolism,vascular constriction,ion transportation,neuronal systems and drug ***,a number of genes coding for proteins involved in cholesterol metabolism were repressed,and some were *** acid biosynthesis appeared to be repressed,whereas fatty acid metabolism appeared to be *** conclusion,using transcriptome profiling analysis,we demonstrated the molecular basis for the pleiotropic effects of GBE50,particularly those involved in lipid *** study provided new clues for further pharmacological study of GBEs.

关键词： Ginkgo biloba extract high-fat diet rat liver regulation transcriptome

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Science China(Life Sciences) 2007年第5期50卷 700-705页

作者： GUI YiJie1, WANG Sheng1, QUAN LiYan1, ZHOU ChangPing2. LONG ShiBao2. ZHENG HuaJun3, JIN Liang1, ZHANG XianYin 1, MA NaiXun 4 & FAN LongJiang1 1 Institute of Crop Science/Institute of Bioinformatics, Zhejiang University, Hangzhou 31002., China 2.Zhejiang Anji Bamboo Exposition Garden, Anji 313300, China 3 chinese national human genome center at shanghai, shanghai 2.12.3, China 4 The Research Institute of Subtropical Forestry, {2. Academy of Forestry, Fuyang 311400, China 1. Institute of Crop Science/Institute of Bioinformatics Zhejiang University Hangzhou 310029 China 2. Zhejiang Anji Bamboo Exposition Garden Anji 313300 China 3. chinese national human genome center at shanghai Shanghai 201203 China 4. The Research Institute of Subtropical Forestry Chinese Academy of Forestry Fuyang 311400 China

Moso bamboo (Phyllostachys pubescens) is one of the world's most important bamboo species. It has the largest area of all planted bamboo―over two-thirds of the total bamboo forest area―and the highest economic value in China. Moso bamboo is a tetraploid (4x=48) and a special member of the grasses family. Although several genomes have been sequenced or are being sequenced in the grasses family, we know little about the genome of the bambusoids (bamboos). In this study, the moso bamboo genome size was estimated to be about 2.34 Mb by flow cytometry (FCM), using maize (cv. B73) and rice (cv. Nipponbare) as internal references. The rice genome has been sequenced and the maize genome is being sequenced. We found that the size of the moso bamboo genome was similar to that of maize but significantly larger than that of rice. To determine whether the bamboo genome had a high proportion of repeat elements, similar to that of the maize genome, approximately 1000 genome survey sequences (GSS) were generated. Sequence analysis showed that the proportion of repeat elements was 2..3% for the bamboo genome, which is significantly lower than that of the maize ge-nome (65.7%). The bamboo repeat elements were mainly Gypsy/DIRS1 and Ty1/Copia LTR retrotrans-posons (14.7%), with a few DNA transposons. However, more genomic sequences are needed to con-firm the above results due to several factors, such as the limitation of our GSS data. This study is the first to investigate sequence composition of the bamboo genome. Our results are valuable for future genome research of moso and other bamboos.

关键词： flow cytometry (FCM), genome size, GSS, Phyllostachys pubescens, repeat elements

水稻逆转录转座子RIRE10拷贝数与LTR区转录活性的鉴定

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生物化学与生物物理学报 2003年第8期35卷 768-773页

作者：王嵘洪国藩韩斌中国科学院上海生命科学研究院国家基因研究中心中国科学院上海生命科学研究院国家基因研究中心上海2.02.3 中国科学院上海生命科学研究院生物化学与细胞生物学研究所上海2.0031

在水稻第四号染色体的长臂上鉴定了一个结构完整的Ty3型逆转录转座子RIRE10。RIRE10两LTR间的中间区域在gag pol的上游还包含另一个开放阅读框。通过RT PCR与Northern印迹杂交检测到来自LTR区的转录产物 ;根据点杂交结果 ,鉴定出包含中... 详细信息

在水稻第四号染色体的长臂上鉴定了一个结构完整的Ty3型逆转录转座子RIRE10。RIRE10两LTR间的中间区域在gag pol的上游还包含另一个开放阅读框。通过RT PCR与Northern印迹杂交检测到来自LTR区的转录产物 ;根据点杂交结果 ,鉴定出包含中间区域的RIRE10成员的个数以及LTR区的拷贝数。除了 6 5个完整的逆转录转座子所具备的两个LTR外 ,水稻基因组还含有近 90 0个RIRE10的solo LTR。LTR区的转录以及导致solo LTR产生的同源重组可能影响了RIRE10成员在水稻基因组中的转座活性

关键词：栽培稻 Ty3型逆转录转座子转录

Design,synthesis,antitumor evaluations and molecular modeling studies of novel 3,5-substituted indolin-2.one derivatives

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Acta Pharmacologica Sinica 2007年第1期28卷 140-152页

作者： Hai-hong LI~2.Xiu-hua ZHANG~3 Jin-zhi TAN~2.Li-li CHEN~2.Hong LIU~(2.5) Xiao-min LUO~(2.5) Xu SHEN~(2.4) Li-ping LIN~(3,5) Kai-xian CHEN~2.Jian DING~3 Hua-liang JIANG~(2.4)2.center for Drug Discovery and Design,State Key laboratory of Drug Research,{2. Institute of Materia Medica,{2. Institutes for Biological Sciences,and Graduate School,chinese Academy of Sciences,shanghai 2.12.3,China 3 Division of Anti-tumor Pharmacology,{2. Institute of Materia Medica,{2. Institutes for Biological Sciences,chinese Academy of Sciences,shanghai 2.12.3,China 4 School of Pharmacy,East China University of Science and Technology,{2. 2.02.7,China centerforDrugDiscoveryandDesign StateKeyLaboratoryofDrugResearchShanghaiInstituteofMateriaMedicaShanghaiInstitutesforBiologicalSciencesandGraduateSchoolChineseAcademyofSciencesShanghai201203China

Aim:To design and synthesize a novel class of antitumor agents,featuring the 3,5-substituted indolin-2.one ***:Based on enzyme binding fea-tures of（Z）-SU5402.introducing a β-pyrrole group at the 3-position of the indolin-2.one core,a series of novel 3,5-substituted indolin-2.ones were designed *** human carcinoma cell lines of A-43l,A-549,MDA-MB-468,and Autosomal Dominant Polycystic Kidney disease were chosen for the cellproliferation ***:Twenty new compounds（1a-t）with E configurationhave been designed,synthesized and *** structural features weredetermined by nuclear magnetic resonance（NMR）spectra,low-and high-resolu-tion mass spectra,and confirmed by X-ray *** the enzymeassay showed a weak inhibition effect against the epidermal growth factor receptor,vascular endothelial growth factor receptor,fibroblast growth factor receptor andplatelet-derived growth factor receptor tyrosine kinases,the cell-based antitumoractivity was *** 1 g and lh showed higher inhibitory activitytoward the A-549 and MDA-MB-468 cell lines with IC50of 0.065-9.4 μmol/***:This study provides a new template for further development ofpotent antitumor drugs.

关键词： protein-tyrosine kinase indolin-2.one antitumor drug screening assays drug design molecular models

中国自身免疫性胰腺炎诊治指南(上海,2.2.)

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临床肝胆病杂志 2024年第7期40卷 1312-1320页

作者：国家消化系统疾病临床医学研究中心(上海) 免疫与炎症全国重点实验室中国医师协会胰腺病学专业委员会中华医学会消化病学分会胰腺疾病学组《中华胰腺病杂志》编辑委员会李兆申李汛郭晓钟廖专辛磊蔡亦李胡良皞李宏宇吴东不详海军军医大学第一附属医院消化内科上海200433 兰州大学第一医院普外科兰州730000 北部战区总医院消化内科沈阳110840 海军军医大学第一附属医院海军特色医学中心北部战区总医院北京协和医院

自身免疫性胰腺炎(AIP)是一种由免疫介导的、特殊类型的慢性胰腺炎,可合并全身多器官的损害,临床表现复杂多样,诊治仍具有一定困难。本指南结合国内外AIP最新指南和研究进展,针对AIP的诊断、治疗、随访和预后4个方面,共提出2.条推荐意见... 详细信息

自身免疫性胰腺炎(AIP)是一种由免疫介导的、特殊类型的慢性胰腺炎,可合并全身多器官的损害,临床表现复杂多样,诊治仍具有一定困难。本指南结合国内外AIP最新指南和研究进展,针对AIP的诊断、治疗、随访和预后4个方面,共提出2.条推荐意见,旨在规范和提高我国AIP的诊治水平,改善患者预后。

关键词：自身免疫性胰腺炎 IgG4相关性疾病指南多学科诊治