检索结果-南通市图书馆

COUP-TFII alters gene profile of atherosclerosis and adhesion function of endothelial cells through multiple mechanisms

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

上海医学 2007年第S1期30卷 175-175页

作者： WUXiaojing SUN Aijun XUDanling WANG Keqiang ZOUYunzeng GEJunbo. shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and institutes of biomedical sciences, fudan university, shanghai 200032, china

Objective Arteries and veins are molecularly distinct. However, whether the intrinsic differences contribute to the different atherosclerosis susceptibility between arteries and veins has remained unknown. This study was to investigate whether COUP-TFII, a key molecular in regulating embryonic arterial-venous differentiation, is implicated in the regulation of processes controlling atherosclerosis. Methods The endothelium of the vein but not the artery of adult vessels in vivo expressed COUP-TFII transcription factor, and the endothelial cells (EC) from human umbilical veins in in vitro culturing began to express some level of arterial EC markers including Jaggedl and Notchl after COUP-TFII was knock down, indicating the key role of COUP-TFII in maintaining of proper arterial-venous demarcation in the adult. Results Gene profiles showed that the venous EC with or without Ang II exposure (1×10-7 mol/L, 4 h) both displayed different gene expression pattern with more atherogenic genes upregulated after COUP-TFII was knock down (P<0.05 and fold change≥2). These genes represented various atherosclerosis-related pathways including lipid transfer and metabolism, adhesion molecular, thrombosis formation, and inflammation pathways. Moreover, the in vitro cell functional assay showed that COUP-TFII knock down in venous EC not only increased the basal but also Ang II induced THP1-EC cell adhesion. Conclusion COUP-TFII influences the gene transcription of atherosclerosis and adhesion function of EC through multiple mechanisms, suggesting, at least at the cellular and gene expression level, that the intrinsic molecular differences between arteries and veins might contribute to their different atherosclerotic susceptibility besides local environment.

关键词： arteries metabolism susceptibility endothelium umbilical inflammation embryonic intrinsic implicated

维普期刊数据库

Recent advances in proteolysis and peptide/protein separation by chromatographic strategies

在线全文

学校读者我要写书评

暂无评论

Science china Chemistry 2010年第4期53卷 685-694页

作者： ZHANG XiangMin1, LIU BaoHong1, ZHANG LiHua2, ZOU HanFa2, CAO Jing1, GAO MingXia1, TANG Jia1, LIU Yun1, YANG PengYuan1 & ZHANG YuKui2 1Department of Chemistry and institutes of biomedical sciences, fudan university, shanghai 200433, china 2Dalian Institute of Chemical Physics, Chinese Academy of sciences, Dalian 116023, china 1. Department of Chemistry and institutes of biomedical sciences Fudan University Shanghai 200433 China2. Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China

This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chroma-tographic strategies in the past ten years, covering the main research in these areas especially in china. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.

关键词： proteolysis enzymatic digestion chromatographic separation peptide enrichment mass spectrometry

维普期刊数据库

High density lipoprotein downregulates angiotensin Ⅱ type Ⅰ receptor and inhibits angiotensin Ⅱ-induced cardiac hypertrophy

在线全文

学校读者我要写书评

暂无评论

High density lipoprotein downregulates angiotensin Ⅱ type ...

第八届海峡两岸心血管科学研讨会

作者： Li Lin,Hui Gong,Jing-yi Ge,Guo-liang Jiang,Ning Zhou,Lei Li, Yong Ye,Guo-ping Zhang,Jun-bo Ge,Yun-zeng Zou (1.shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,fudan university, 180 Feng Lin Road,shanghai 200032,china 2.institutes of biomedical Science,fudan university,shanghai 200032,china)

＜正＞Backgroud Angiotensin Ⅱ （Ang Ⅱ ） and its type receptor（AT1-R） play important roles in the development of cardiac *** level of high density lipoprotein（HDL） is also an independent risk factor for cardiac *** therefore investigated in the present study whether HDL inhibits cardiac hypertrophy relatively to inhibition of Ang Ⅱ and AT1-R in both in vitro and in vivo *** and Results Stimulation of cultured cardiomyocytes of neonatal rats with Ang Ⅱ for 24 h and infusion of Ang Ⅱ in mice for 2 weeks resulted in marked cardiac hypertrophic responses including increased protein synthesis,enlarged sizes of cardiomyocytes and hearts,upregulated phosphorylation levels of protein kinases and reprogrammed expression of specific genes,all of which were significantly attenuated by the treatment with HDL. Furthermore,Ang Ⅱ -treatment induced upregulation of AT-R expression either in cultured cardiomyocytes or in hearts of mice and HDL significantly suppressed the upregulation of *** Our results suggest that HDL may abrogate Ang Ⅱ -induced cardiac hypertrophy through downregulation of AT1-R expression.

关键词：

Con A affinity glycoproteomics of normal human liver tissue

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science china(Life sciences) 2007年第3期50卷 403-411页

作者： SUN QiangLing1, LIU YinKun1, LU WenJing1, CHENG Gang2, ZHOU HaiJun1, ZHOU XinWen3, WEI LiMing3, DAI Zhi1, GUO Kun1 & LU HaoJie3 1 Liver Cancer Institute, Zhongshan Hospital, fudan university, shanghai 200032, china 2 System Biology Lab, Institute of biomedical sciences, fudan university, shanghai 200433, china 3 Research Center for Proteomics, Institute of biomedical sciences, fudan university, shanghai 200433, china 1. Liver Cancer Institute Zhongshan Hospital Fudan University Shanghai 200032 China 2. System Biology Lab Institute of Biomedical Sciences Fudan University Shanghai 200433 China 3. Research Center for Proteomics Institute of Biomedical Sciences Fudan University Shanghai 200433 China

In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue were gener- ated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence stain- ing based on multiplexed proteomics (MP) technology. 301 visible protein spots on the gel were de- tected and 85 of glycoproteins were further successfully identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF- MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal human liver tissue would contribute to the subsequent research.

关键词： Con A affinity glycoproteomics of normal human liver tissue

维普期刊数据库

Cofilin rod formation and its regulation:a pilot study by using live cell imaging system

在线全文

学校读者我要写书评

暂无评论

Cofilin rod formation and its regulation:a pilot study by us...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yun WANG institutes of Brain Science and State Key Laboratory for Medical Neurobiology,fudan university,shanghai 200032,china

＜正＞Neurite transport defects have been linked to very early stages of many neurodegenerative ***,a protein regulates dynamics of the actin cytoskeleton,has been reported to form rod structure altering neurite transport in pathological condition such as Alzheimer’s *** aim of this study is by using live cell station to investigate the dynamic change of the cofilin rod formation and it’s regulating cellular *** hippocampal neurons were either transfected with cofilin-GFP alone or co-transfected with cofilin-GFP and LIM JCinases/RFP and then were under long term continuous observation（＞10 h） by using Ultraview VoX live cell scanning unit（PerkinElmer Life sciences）.Timelapse confocal image showed neurons transfected with cofilin developed to form rod structure,in distal dendrites but not axon,in a dynamic way:rods elongating,splitting,merging,and moving in a local area,in first 24 h after *** more mature neurons and 2-3 days after transfection,cofilin rods were much more *** cofilin rods were stably formed,neurons co-transfected with LIMK/RFP showed time-dependent dissolving of formed rods in cultured *** conclusion,our results demonstrate that cofilin rod is formed in a dynamic way in distal dendrites and regulated by LIM *** relation between cofilin rod and some neurological disorders are currently under investigation.

关键词： cofilin cofilin rod LIM Kinases live cell station

Inhibition of cation-chloride co-transporter prolonged epileptogenesis

在线全文

cnki会议

学校读者我要写书评

暂无评论

Inhibition of cation-chloride co-transporter prolonged epile...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Bin-Bin QIAN Zhen WU Yun WANG~* institutes of Brain Science fudan university shanghai 200032 china

＜正＞Objective GABA receptor function is important in epileptogenesis which is mainly by regulation of chloride ion ***-chloride co-transporters subtype potassium-chloride co-transporter 2（KCC2） is a key factor in adult rats in regulating this concentration *** study showed a down-regulation of KCC2 during *** aim of this study is to investigate the functional role KCC2 in *** PTZ induced acute epilepsy model was used and field potential of hippocampus CA1 region was recorded in anaesthetized ***,a known nonselective KCC2 inhibitor,was tested on PTZ epilepsy ***（1） A single high dose of PTZ induced progressive epileptiform CA1 neuronal activity consistent with multiple evoked population spike（PS） peaks to highly synchronized bursts.（2） Furosemide（40mg/kg） significantly prolonged the latency to evoke PS double peaks and inhibited triple peaks but without effect on bursts induced by *** Our results demonstrate that inhibition of KCC2 will slow down epileptogenesis progress.

关键词： epilepsy chloride ion co-transporter KCC2 furosemide

Neural stem/progenitor cells in the postnatal subventricular zone give rise not only to granular and periglomerular interneurons,but also to interneurons in the external plexiform layer of the rat olfactory bulb

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural stem/progenitor cells in the postnatal subventricular...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Zhengang YANG institutes of Brain Science and State Key Laboratory of Medical Neurobiology,fudan university,shanghai 200032,china

＜正＞Interneurons in the granule cell layer（GCL） and glomerular layer（GL） of the olfactory bulb（OB） are generated from neural stem/progenitor cells in the subventricular zone（SVZ） of the lateral ***,little is known about the origin of interneurons in the external plexiform layer（EPL） of the *** the basis of the concept of corticogenesis,we hypothesized that interneurons in the EPL of the rodent OB also originate in the *** the present study,replicationincompetent retroviruses encoding the marker gene,human placental alkaline phosphatase（AP）,were injected into the lateral ventricles of postnatal day 4 Wistar rats to label dividing cells in the *** days after injection,AP labeled cells had migrated into the *** weeks after injection,AP/NeuN double labeled cells were found not only in the GCL and GL, but also in the EPL of the *** the EPL,most AP labeled cells were calcium-binding protein parvalbumin-（PV） immunoreactive （+） interneurons.A subset of these cells were calcium-binding protein calretinin（CR） + *** to their structural features,these AP/PV double labeled cells were Van Gehuchten cells,multipolar cells and superficial shortaxon ***,the neural stem/progenitor cells in the postnatal SVZ give rise not only to granular and periglomerular interneurons,but also to interneurons in the EPL of the ***,these data suggest that that SVZ-derived progenitors give rise to virtually all subpopulations of interneurons in the OB.

关键词： subventricular zone olfactory bulb external plexiform layer neurogenesis parvalbumin calretinin

Role of KCC2 in seizure induction and intervention

在线全文

cnki会议

学校读者我要写书评

暂无评论

Role of KCC2 in seizure induction and intervention

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yun WANG institutes of Brain Science and State Key Laboratory for Medical Neurobiology,fudan university,shanghai 200032, china

The neuron specific K-Cl cotransporter KCC2 in adult plays an important role to maintain intracellular Cl homeostasis,hence renders GABA receptor inhibitory ***,KCC2 expression levels are found to be reduced in various pathological conditions,including *** current study,we analyzed KCC2 expression and function and its involvement in GABA receptor mediated inhibition change during acute seizure *** using cyclothiazide(CTZ) induced both in vivo and in vitro acute seizure model,we discovered that the hippocampal neuron somatic membrane KCC2 expression was down regulated,the GABA reversal potential(E) was up- shifted and a profound reduction in Cl extrusion *** molecular manipulation of KCC2 expression in cultured hippocampal neurons,CTZ treatment induced epileptic neurons was significantly reduced in association with KCC2 over expression,and KCC2 down regulated by shKCC2 resulted a E up-shift but without effect on CTZ induction of epileptiform *** addition,furosemide,a KCC2 inhibitor,while applied continuously mimicked CTZ effect to cause the E up-shift and facilitate the CTZ induced epileptiform ***,furosemide co-treated with CTZ prevented CTZ induced KCC2 down regulation from the somatic membrane and in turn rescued acute convulsant stimulation induced GABA inhibitory function *** conclusion,our data suggest that KCC2 plays an important role in seizure induction and prevent KCC2 down regulation may be a novel target for anticonvulsant development.

关键词： CTZ epilepsy hippocampus KCC2 GABAA receptor

Cloning and characterization of a mouse liver-specific gene mfrep-1, up-regulated in liver regeneration

在线全文

cnki会议

学校读者我要写书评

暂无评论

Cell Research 2002年第Z2期 353-361页

作者： JUN VAN, HAD YING, FBI GU, JIN HE, Yu Li LI, Hm MIN LIU, YONG HUA XU Laboratory of Molecular and Cellular Oncology, Institute of Biochemistry and Cell Biology, shanghai institutes for Biological sciences, Chinese Academy of sciences, 320 Yueyang Road, shanghai 200031, china Department of Surgery, Huashan Hospital, fudan university, shanghai 200031, china Department of Pathology, Changzheng Hospital, Second Military Medical university, shanghai 200003, china

Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-l/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-l/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-l/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice, mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced express

关键词： mfrep-1 in silico cloning liver regeneration liver-specific expression.