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Role of inositol 1,4.5-trisphosphate receptors in α1-adrenergic receptorinduced cardiomyocyte hypertrophy

Acta Pharmacologica Sinica 2006年第7期27卷 895-900页

作者： Da-li LUO~(2,3.5) Jian GAO~2 Xiao-mei LAN~3.Gang WANG~4.Sheng WEI~4.Rui-ping XIAO~4.Qi-de HAN~3. Department of Pharmacology,School of Chemical Biology & Pharmaceutical sciences,Capital {4. of Medical sciences,{3. 100069,China 3.{4. of Cardiovascular science at Health science Center,Peking {4. {3. 100083.China {14.,Peking {4.,beijing 100871,China Department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences Beijing 100069 China

Aim:Intracellular Ca2+plays pivotal roles in diverse cellular functions,includinggene transcription that underlies cardiac remodeling during stress ***,the role of inositol 1,4.5-trisphosphate receptors（IP3.s）in the media-tion of cardiac intracellular Ca2+and hypertrophic growth remains *** with neonatal rat ventricular myocytes suggests that activation of IP3.smay be linked to α1adrenergic receptor（α1AR）increased stereotyped Ca2+sparkoccurrence and global Ca2+***,we hypothesized that Ca2+releasethrough IP3.s was necessary for α1AR-stimulated cardiac ***:We used myoinositol 1,4.5-trisphosphate hexakis（butyryloxymethyl）ester（IP3.M）,a membrane-permeant ester of IP3.to activate IP3.s directly,and Fluo 4.AM tomeasure intracellular Ca2+***:IP3.M(10μmol·L-1)mimicked theeffects of phenylephrine,a selective agonist of α1AR,in increments in local Ca2+spark release（especially in the perinuclear area）and global Ca2+*** importantly,IP3. inhibitors,2-aminoethoxydiphenyl borateand Xestospongin C,abolished the IP3.M-induced Ca2+responses,and signifi-cantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cellsize,[3.]leucine incorporation and atrial natriuretic factor gene expression,dur-ing sustained（4. h）phenylephrine ***:These results,therefore,provide cellular mechanisms that link IP3. signaling to α1AR-stimulatedgene expression and cardiomyocyte hypertrophy.

关键词： inositol 1,4.5-trisphosphate receptors Ca2+sparks α1 adrenergic stimulation cardiac hypertrophy

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Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA

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science China Chemistry 2006年第1期49卷 75-80页

作者： ZHANG Huijuan1,2, WANG Jin3. WU Yishi1, YUAN Gu3. AI Xicheng1, WANG Li4.& ZHANG Jianping1 1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, institute of Chemistry, Chinese Academy of sciences, {4. 100080, China 2. School of {3. science and Technology, {4. institute of Technology, {4. 100081, China 3. college of Chemistry and molecular Engineering, Peking university, {4. 100080, China 4. Center for Condensed Matter Physics, institute of Physics, Chinese Academy of sciences, {4. 100080, China Correspondence should be addressed to Ai Xicheng and Zhang Jianping State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing China School of life science and Technology Beijing Institute of Technology Beijing China college of Chemistry and molecular Engineering Peking University Beijing China Center for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing China

The photophysical properties of the polyamide PyPyPyβDp (PPP) were investigated by means of steady-state absorption and fluorescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. It was found that the excited-state properties of PPP are very sensitive to solvents. In TKMC buffer PPP exhibited weak fluorescence with a decay time constant of 16 ps, while with the decrease of the solvent polarity PPP showed the blue-shifted peak position, increased inten-sity and lengthened life-time for its fluorescence behavior. In the presence of calf thymus DNA, it was observed that the fluorescence intensity was enhanced and the fluorescence lifetime increased from 16 to 3. ps for PPP, which verified that PPP bound into the minor groove of DNA duplex.

关键词： polyamide (PPP), calf thymus DNA (ctDNA), time-resolved fluorescence spectroscopy, recognition dynamics.

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A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

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Chinese Chemical Letters 1999年第2期10卷 121-122页

作者： Mei DU Sheng JIN Zhen Quan GUO(college of Chemistry and molecular Engineering. Peking university. {3.)(college of life science, Peking university, {3.) Peking Univ Coll Chem & Mol Engn Beijing 100871 Peoples R China Peking Univ Coll Life Sci Beijing 100871 Peoples R China

The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3.× 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.

关键词： Monoclonal antibody carboxypeptidase A (CPA) competitive inhibition peptidaseactivity

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Development and application of ergodicity model with FRCM and FLAR for hydrological process

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science China(Technological sciences) 2009年第2期52卷 379-386页

作者： WANG HongRui1, FENG QiLei2, LIN Xin3.& ZENG WenYi4.1 college of Water science, Key Laboratory for Water and Sediment sciences of the Ministry of Education, {3. Normal {4., {3. 100875, China 2 School of science, {3. institute of Education, {3. 100011, China 3.School of Mathematical sciences, {3. Normal {4., {3. 100875, China 4.college of Information science and Technology, {3. Normal {4., {3. 100875, China School of science Beijing Institute of Education School of Mathematical sciences Beijing Normal University college of Information science and Technology Beijing Normal University

In this paper, a new ergodic property analysis model of hydrological process is proposed based on fuzzy-rough c-means clustering (FRCM), autocorrelogram, and fuzzy least absolute regression (FLAR). A precipitation time series (1951―2004. from Shanghai Hydrology Station is then analyzed with the model. The results show that the precipitation time series of April, May, June, and September has er-godic property. We conclude that in the long run, the precipitation of April, May, June, and September will not keep decreasing; it will converge to its mean value in some period.

关键词： hydrological process ergodic autocorrelogram fuzzy-rough c-means clustering fuzzy least absolute regression

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PROPERTIES OF POLYURETHANE ELASTOMERS BASED ON POLY (OXYETHYLENE-co-OXYTETRAMETHYLENE) DIOL

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Chinese Journal of Polymer science 2001年第4期19卷 371-376页

作者： Chen-yu Su Guang-li Zhang Hong-zhi Zhang Department of Polymer science and Engineering college of Chemistry and molecular Engineering Peking {3. {4., China 北京大学北京

Polyurethane elastomers derived from 4.4.' -methylene bis(phenyl isocyanate), butylene glycol and the low oxyethylene moiety content copolyether of tetrahydrofuran and ethylene oxide, obtained by copolymerization using heteropolyacid-ethylene oxide initiator system, were prepared. The polyurethanes exhibited an increased water absorption and much better low temperature resilience, which remained high even at a temperature of -3.degreesC, while that of polyurethane based upon poly(tetramethylene ether) glycol of the 'same molecular weight, i.e. being 2000, and molecular weight distribution, i.e, in the range of 1.4.1.5, starts to decrease dramatically at 5 degreesC.

关键词： polyurethane poly(oxyethylene-co-oxytetramethylene) diol low temperature resilience heteropolyacid-ethylene oxide initiator system

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Theoretical study on the reaction mechanism of(CH_3._3.O+CO

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science China Chemistry 2005年第1期48卷 18-24页

作者： ZHAO Hongmei1,2,3. SUN Chengke1,4. ZHANG Rongchang3. XI Hongmin3.& LI Zonghe1 1. Department of Chemistry, beijing Normal {4., beijing 100875, China 2. The Laboratory of {3. Reaction Dynamics, {4. of Chemistry, Chinese Academy of sciences, beijing 100080, China 3. Department of Chemistry, Beihua {4., Jilin 13.013. China 4. Department of Chemistry, Qujing Normal {4., Yunnan 655000, China Department of Chemistry Beijing Normal University Beijing China The Laboratory of molecular Reaction Dynamics Institute of Chemistry Chinese Academy of Sciences Beijing China Department of Chemistry Beihua University Jilin China Department of Chemistry Qujing Normal University Yunnan China

The reaction mechanism of (CH3.3.O with CO has been theoretically investigated using density-functional theory (DFT) calculations at B3.YP/6-3.G* level. In order to get more reliable energy values the single-point energy is evaluated at CCSD (T)/6-3.++G** level. The results show that the reaction is multi-channel and the reaction of (CH3.3.O radical with CO mostly produces (CH3.3. + CO2. The reaction could play a role in eliminating air pollution.

关键词： DFT, B3.YP, (CH3.3.O, radical.

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Role of gallium wetting layer in high-quality ZnO growth on sapphire (0001) substrates

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science China(Physics,Mechanics & Astronomy) 2004年第5期47卷 612-620页

作者： ZENG Zhaoquan1,3. WANG Yong2, DU Xiaolong1, MEI Zengxia1, KONG Xianghe3. JIA Jinfeng1, XUE Qikun1 & ZHANG Ze2, 4.1. State Key Laboratory for Surface Physics, institute of Physics, Chinese Academy of sciences, {4. 100080, China 2. Laboratory of Electron Microscopy, institute of Physics, Chinese Academy of sciences, {4. 100080, China 3. college of Physics and Engineering, Qufu Normal university, Qufu 273.65, China 4. {4. university of Technology, {4. 100022, China State Key Laboratory for Surface Physics Institute of Physics Chinese Academy of Sciences Beijing China college of Physics and Engineering Qufu Normal University Qufu China Laboratory of Electron Microscopy Institute of Physics Chinese Academy of Sciences Beijing China beijing university of Technology Beijing China

A Ga wetting layer was used to modify the surface structure of sapphire (0001) substrate to prepare high-quality ZnO film by radio frequency plasma-assisted molecule beam epitaxy. We found that this Ga layer plays a crucial role in eliminating 3. rotation domains, controlling polarity and decreasing defect density in ZnO epilayers, as demonstrated by in situ reflection high energy electron diffraction, ex situ high resolution X-ray diffraction and high resolution cross-sectional transmission electron microscopy. Zn-polar film of ZnO was determined by convergent beam electron diffraction. A Ga bilayer model is proposed to understand the effects of the Ga wetting layer on high-quality ZnO growth.

关键词： Ga wetting layer, sapphire, polarity, defect density, zinc oxide, rf-MBE, RHEED, HRXRD, TEM, CBED.

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Monitoring of rice crop using ENVISAT ASAR data

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science China Earth sciences 2006年第7期49卷 755-763页

作者： DONG Yanfang1, 2, SUN Guoqing1,3.& PANG Yong4.1. State Key Laboratory of Remote Sensing science, institute of Remote Sensing Applications, Chinese Academy of sciences, {3. 100101, China 2. Graduate {4. of Chinese Academy of sciences, {3. 10003., China 3. Department of Geography, {4. of Maryland, college Park, MD 2074., USA 4. Forest Remote Sensing Lab, Research institute of Forest Resource and Information Technology, Chinese Academy of For-estry, {3. 100091, China State Key Laboratory of Remote Sensing science Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing China Graduate university of Chinese Academy of sciences Beijing China Department of Geography University of Maryland College Park USA Forest Remote Sensing Lab Research Institute of Forest Resource and Information Technology Chinese Academy of Forestry Beijing China

Because of the cloudy conditions during the rice growth period, rice is one of the agri-cultural crops most suited to monitoring with the SAR instruments. Backscatter response measured by SAR is correlated with rice conditions, including height, density, biomass and structure, which are variable at different growing stages. In this paper, multi-date ENVISAT ASAR Alternating Polarization Mode (APMode) imageries were acquired during the rice crop growing cycle. At the same time, the rice parameters were measured in field. A continuous canopy model was used to compute the back-scattering from rice fields during the growth cycle, and the relationship between rice parameters and radar backscattering coefficients from both ASAR and modeling was analyzed. The effects of polari-zation, incidence angle and polarization on radar backscattering coefficients were analyzed. It was found that simulated radar backscatter has similar trends as ASAR data. This will be meaningful for the further research of rice parameters estimation from ASAR data. Different features show signifi-cantly different signatures in ASAR images and they follow some certain laws, so rice area can be accurately mapped by using multi-temporal SAR images, then rice yield can be estimated.

关键词： rice, ASAR, radar backscattering, model.

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LOD score exclusion analyses for candidate disease susceptibility genes using case-parents design

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Progress in Natural science:Materials International 2006年第1期16卷 30-38页

作者： DENG Hongwen1,2,3.and GAO Guimin2,4.(1.Laboratory of molecular and Statistical Genetics, college of life {3.s, Hunan Normal {4., Changsha 4.0081, China 2.Osteoporosis Research Center, Creighton {4. Medical Center, Omaha NE 6813., USA 3. Department of Biomedical {3.s, Creighton {4., Omaha NE 6813., USA 4. Virginia Bioin format ics institute and Department of Statistics, Virginia Polytechnic institute and State {4., Blacksburg VA 24.61-03.5, USA) Laboratory of molecular and Statistical Genetics College of Life Sciences Hunan Normal University Changsha 410081 China

The focus of almost all the association studies of candidate genes is to test for their importance. We recently developed a LOD score approach that can be used to test against the importance of candidate genes for complex diseases and quantitative traits in random samples. As a complementary method to regular association analyses, our LOD score approach is powerful but still affected by the population admixture, though it is more conservative. To control the confounding effect of population heterogeneity, we develop here a LOD score exclusion analysis using case-parents design, the basic design of the transmission disequilibrium test (TDT) approach that is immune to population admixture. In the analysis, specific genetic effects and inheritance models at candidate genes can be analyzed and if a LOD score is ≤ - 2. 0, the locus can be excluded from having an effect larger than that specified. Simulations show that this approach has reasonable power to exclude a candidate gene having small genetic effects if it is not a disease susceptibility locus (DSL) with sample size often employed in TDT studies. Similar to association analyses with the TDT in nuclear families, our exclusion analyses are generally not affected by population admixture. The exclusion analyses may be implemented to rule out candidate genes with no or minor genetic effects as supplemental analyses for the TDT. The utility of the approach is illustrated with an application to test the importance of vitamin D receptor (VDR) gene underlying the differential risk to osteoporosis.

关键词： association studies candidate genes case-parents design disease susceptibility locus exclusion mapping LOD score.

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Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure

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science China(life sciences) 2006年第2期49卷 97-104页

作者： SHEN Sile1,3. WANG Zhenwei3. SHAN Xiaohui2, WANG Hua3. LI Ling3. LIN Xuyun2, LONG Likun2, WENG Kenan4. LIU Bao2 & ZOU Guangtian1 1. National Laboratory of Superhard Materials, Jilin {4., Changchun 13.012, China 2. institute of Genetics and Cytology, Northeast Normal {4., Changchun 13.024. China 3. {3. of {4. science, Jilin {4., Changchun 13.012, China 4. Guangzhou institute of Geochemistry, Chinese Academy of sciences, Guangzhou 51064., China National Laboratory of Superhard Materials Jilin University Changchun China college of life science Jilin University Changchun China institute of Genetics and Cytology Northeast Normal University Changchun China Guangzhou institute of Geochemistry Chinese Academy of Sciences Guangzhou China

By using high-pressure treatment, two mutant lines were obtained from agenetically stable japonica rice cultivar Bijing3.. Genomic DNA of the mutant lines, together withthe original line (Bijing3.), was either undigested or digested by Hpa ⅡIMspⅠ, and then subjectedto molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCRamplification profiles of both markers are apparent in the two mutant lines compared with theoriginal rice cultivar, suggesting that there had been both sequence changes and DNA methylationmodifications in the mutant lines. Southern blot analysis using diverse sequences, including twocellular genes (S2 and S3., a set of retrotransposons (Osr7, Osr3., Tos19 and more), and a MITEtransposon family (mPing and Pong), confirmed the results, and indicated that changes in DNAmethylation pattern, genomic structure, and possible activation of some transposons indeed occurredin the mutant lines. Moreover, these changes are stably maintained through selfed generations and indifferent organs. Thus, our results indicate that it is possible to obtain stable mutants in riceby high pressure treatments, and the molecular basis of the mutants may include both genetic andepigenetic changes. Therefore, high hydrostatic pressure seems a promising approach for plantmutagenesis.

关键词： DNA methylation, genomic changes, high pressure, mutagenesis, rice.

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