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Role of inositol 1,4,5-trisphosphate receptors in α1-adrenergic receptorinduced cardiomyocyte hypertrophy

Acta Pharmacologica Sinica 2006年第7期27卷 895-900页

作者： Da-li LUO~(2,3.5) Jian GAO~2 Xiao-mei LAN~3.Gang WANG~4 Sheng WEI~4 Rui-ping XIAO~4 Qi-de HAN~3. Department of Pharmacology,School of Chemical Biology & Pharmaceutical Sciences,Capital university of Medical Sciences,beijing 100069,china 3.{3. of Cardiovascular Science at Health Science Center,peking university beijing 100083.china 4.institute of molecular medicine and college of life Science,peking university,{13.,china Department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences Beijing 100069 China institute of Cardiovascular Science at Health Science Center Peking University Beijing 100083 China institute of molecular medicine and {3. of life Science Peking University Beijing 100871 China

Aim:Intracellular Ca2+plays pivotal roles in diverse cellular functions,includinggene transcription that underlies cardiac remodeling during stress ***,the role of inositol 1,4,5-trisphosphate receptors（IP3.s）in the media-tion of cardiac intracellular Ca2+and hypertrophic growth remains *** with neonatal rat ventricular myocytes suggests that activation of IP3.smay be linked to α1adrenergic receptor（α1AR）increased stereotyped Ca2+sparkoccurrence and global Ca2+***,we hypothesized that Ca2+releasethrough IP3.s was necessary for α1AR-stimulated cardiac ***:We used myoinositol 1,4,5-trisphosphate hexakis（butyryloxymethyl）ester（IP3.M）,a membrane-permeant ester of IP3.to activate IP3.s directly,and Fluo 4/AM tomeasure intracellular Ca2+***:IP3.M(10μmol·L-1)mimicked theeffects of phenylephrine,a selective agonist of α1AR,in increments in local Ca2+spark release（especially in the perinuclear area）and global Ca2+*** importantly,IP3. inhibitors,2-aminoethoxydiphenyl borateand Xestospongin C,abolished the IP3.M-induced Ca2+responses,and signifi-cantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cellsize,[3.]leucine incorporation and atrial natriuretic factor gene expression,dur-ing sustained（48 h）phenylephrine ***:These results,therefore,provide cellular mechanisms that link IP3. signaling to α1AR-stimulatedgene expression and cardiomyocyte hypertrophy.

关键词： inositol 1,4,5-trisphosphate receptors Ca2+sparks α1 adrenergic stimulation cardiac hypertrophy

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Biotic communities of the marine ecosystem in Meizhou Bay

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Journal of Environmental Sciences 1996年第1期8卷 48-56页

作者： Ren Jiuchang Cai Xiaoming (Center of Environmental Sciences,peking university, beijing 100871,china)Chen Bin({3. of life Sciences, peking university, beijing 100871, china )Li Peng({3. of Chemistry and molecular Engineering, peking university, Bei Ctr of Envmtl Sci Peking Univ Beijing China China

The current situation of biotic communities in Meizhou Bay is presented in this *** species composition,seasonal variation,and distribution of phytoplankton,zooplankton, red tide organisms,fishes,and benthos in Meizhou Bay were investigated,and the content of chlorophyll a and primary production and their seasonal variation were also *** water quality of Meizhou Bay was monitored by measuring the density of Escherichia coli. Results reveal that there is a great variety of species in Meizhou Bay and the water in Meizhou Bay is *** the individual numbers of various organisms are very low, especially those of ***,there are more than 40 species of red tide organisms and there exist opportunities of red tides between May and *** these demand cautious be taken in the future development of this area although it has great environment capacity.

关键词： biotic community marine ecosystem Meizhou Bay.

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Structural Analysis and Identification of Cis-Elements of Rice osRACD Gene

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 191-198页

作者： Yun-Zhe XU, Rui-Lin YOU and Nai-Hu WU institute of Genetics and Developmental Biology, Chinese Academy of Sciences, beijing 100080, china 2{3. of life Sciences, peking university, beijing 100871, china institute of Genetics and Developmental Biology Chinese Academy of Sciences college of life Sciences Peking University

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4 kb in 5' upstream region of the osRACD promoter. Deletion mutation and gel mobility shift assay characterized two fragments (–799 to –686 nt, and –686 to –43. nt) in the osRACD promoter that could be involved in its transcriptional regulation. When these two deletion fragments were used as probe respectively, a retarded band appeared in the nuclear extracts of fertile 58S rice under short day (58S-SD). Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day (58S-LD). Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor (termed rice factor, RF). The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.

关键词： photoperiod-sensitive male genic sterile rice osRACD gene photoperiod fertility transformation cis-element

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A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

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Chinese Chemical Letters 1999年第2期10卷 121-122页

作者： Mei DU Sheng JIN Zhen Quan GUO(college of Chemistry and molecular Engineering. peking university. {3.)(college of life Science, peking university, {3.) peking Univ Coll Chem & Mol Engn Beijing 100871 Peoples R China peking Univ Coll Life Sci Beijing 100871 Peoples R China

The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3.× 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.

关键词： Monoclonal antibody carboxypeptidase A (CPA) competitive inhibition peptidaseactivity

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Research progress in gecko locomotion and biomimetic gecko-robots

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Progress in Natural Science:Materials International 2007年第1期17卷 1-5页

作者： DAI Zhendong and SUN Jiurong ( institute of Bio-inspired Structure and Surface Engineering, Nanjing university of Aeronautics and Astronautics, Nanjing 210016, china {3. of life Sciences, peking university, beijing 100871, china) [a]institute of Bio-inspired Structure and Surface Engineering Nanjing University of Aeronautics and Astronautics Nanjing China [b]college of life Sciences Peking University Beijing China

Geckos are known for their excellent ability to climb walls and run on ceilings. Previous studies of the gecko's locomotive and adhesive mechanisms, its neuro-sensory and neuro-modulatory systems, its fabrication of artificial setae array, and other related developments, have inspired further research on gecko-based and gecko-like robots. Key research findings in this area are reviewed in the present paper.

关键词： gecko locomotion biomimetic modulation gecko-like robot.

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Status of study on biological and toxicological effects of nanoscale materials

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Science china Chemistry 2005年第5期48卷 385-394页

作者： WANG Bing1, FENG Weiyue1, ZHAO Yuliang1, XING Gengmei1, CHAI Zhifang1, WANG Haifang2 & JIA Guang3.1. Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials (Nanosafety Lab), institute of High Energy Physics, Chi-nese Academy of Sciences, beijing 100049, china 2. {3. of Chemistry and molecular Engineering, peking university, beijing 100871, china 3. Department of Occupational and Environmental Health Sciences, School of Public Health, peking university, beijing 100083. china 1. Laboratory for Bio-Environmental Health Sciences of Nanoscale Materials (Nanosafety Lab) Institute of High Energy Physics Chinese Academy of Sciences 100049 Beijing China 2. college of Chemistry and molecular Engineering Peking University 100871 Beijing China 3. Department of Occupational and Environmental Health Sciences School of Public Health Peking University 100083 Beijing China

Because the physical and chemical properties of nanosized materials mostly differ from the existing microsized materials, their potential impacts on human health and the envi-ronment will be topics under the serious discussions in press and in a number of international scientific journals. We analyze and summarize the existing data of the experimental study on the biological activities and adverse effects of nanoscale materials/particles including single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron powders. Though some biological behaviors of nanoscale materials observed cannot be understood on the basis of the current knowledge, as the existing data are mostly preliminary, it is too early to make some ex-clusive conclusions on biological activities (or the toxicity) of any of nanoscale materials. The experimental techniques, the current topics, and the future research directions for this new re-search field are also discussed.

关键词： nanomaterials, single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron, biological activity and toxicological effect.

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Systematic study of the transfer of amino acids across the water/1,2-dichloroethane interface facilitated by dibenzo-18-crown-6

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Science china Chemistry 2004年第1期47卷 24-33页

作者： CHEN Yong, YUAN Yi, ZHANG Meiqin, LI Fei, SUN Peng, GAO Zhao & SHAO YuanhuaState Key Laboratory of Electroanalytical Chemistry, Changchun institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 13.022, china college of Chemistry and {3. Engineering, peking university, beijing 100871, china Lab. of Electroanalytical Chem. Changchun Inst. of Appl. Chem. Chinese Acad. of Sci. Changchun 130022 China Coll. of Chem. and molecular Eng. Peking Univ. Beijing 100871 China

Facilitated ion transfer reactions of 20 amino acids with dibenzo-18-crown-6 (DB18C6) at the water/1,2-dichloroethane (W/DCE) interfaces supported at the tips of micro- and nano-pipets were investigated systematically using cyclic voltammetry. It was found that there were only 10 amino acids, that is, Leu, Val, lie, Phe, Trp, Met, Ala, Gly, Cys, Gin (in brief), whose protonated forms as cations can give well-defined facilitated ion transfer voltammograms within the potential window, and the reaction pathway was proven to be consistent with the transfer by interfacial complexation/dissociation (TIC/TID) mechanisms. The association constants of DB 18C6 with different amino acids in the DCE (β0), and the kinetic parameters of reaction were evaluated based on the steady-state voltammetry of micro- or nano-pipets, respectively. The experimental results demonstrated that the selectivity of complexation of protonated amino acid by DB18C6 compared with that of alkali metal cations was low, which can be attributed to the vicinal effect arising from steric hindrance introduced by their side group and the steric bulk effect by lipophilic stabilization. Moreover, the association constants and the standard rate constants for different amino acids showed good correlations with their hydrophobicity (π), except Gly and Met, which inferred that the selectivity of such heterogeneous complex reaction for different amino acids with DB18C6, was not only affected by discrimination in binding these ions to the crown ether macro-cycle, but also significantly modified by the ion transfer Gibbs energy which was closely related to the structure of the transferred ions, protonated amino acids.

关键词： micro-/nano-pipet, water/1,2-dichloroethane interface, facilitated ion transfer, cyclic voltammetry, amino acid.

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Fluorescence dynamics of interactions between polyamide PyPyPyβDp and DNA

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Science china Chemistry 2006年第1期49卷 75-80页

作者： ZHANG Huijuan1,2, WANG Jin3. WU Yishi1, YUAN Gu3. AI Xicheng1, WANG Li4 & ZHANG Jianping1 1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, institute of Chemistry, Chinese Academy of Sciences, beijing 100080, china 2. School of {3. Science and Technology, beijing institute of Technology, beijing 100081, china 3. college of Chemistry and molecular Engineering, peking university, beijing 100080, china 4. Center for Condensed Matter Physics, institute of Physics, Chinese Academy of Sciences, beijing 100080, china Correspondence should be addressed to Ai Xicheng and Zhang Jianping 1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 2. School of life Science and Technology Beijing Institute of Technology Beijing 100081 China 3. college of Chemistry and molecular Engineering Peking University Beijing 100080 China 4. Center for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The photophysical properties of the polyamide PyPyPyβDp (PPP) were investigated by means of steady-state absorption and fluorescence spectroscopies, as well as time-resolved fluo-rescence spectroscopy. It was found that the excited-state properties of PPP are very sensitive to solvents. In TKMC buffer PPP exhibited weak fluorescence with a decay time constant of 16 ps, while with the decrease of the solvent polarity PPP showed the blue-shifted peak position, increased inten-sity and lengthened life-time for its fluorescence behavior. In the presence of calf thymus DNA, it was observed that the fluorescence intensity was enhanced and the fluorescence lifetime increased from 16 to 3. ps for PPP, which verified that PPP bound into the minor groove of DNA duplex.

关键词： polyamide (PPP), calf thymus DNA (ctDNA), time-resolved fluorescence spectroscopy, recognition dynamics.

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Applications of functionalized ionic liquids

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Science china Chemistry 2006年第5期49卷 385-401页

作者： LI Xuehui1, ZHAO Dongbin2,3. FEI Zhaofu2 & WANG Lefu1 1. Department of Chemical Engineering, Guangdong Provincial Laboratory of Green Chemical Technology, South china Uni-versity of Technology, Guangzhou 510641, china 2. Swiss Federal institute of Technology, Lausanne, EPFL, CH-1015 Lausanne, Switzerland 3. PKU Green Chemistry Center, {3. National Laboratory for molecular Sciences, college of Chemistry and molecular Engineering, peking university, beijing 100871, china 1. Department of Chemical Engineering Guangdong Provincial Laboratory of Green Chemical Technology South China University of Technology Guangzhou 510641 China 2. Lausanne EPFL Swiss Federal Institute of Technology CH-1015 Lausanne Switzerland 3. PKU Green Chemistry Center Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

Recent developments of the synthesis and applications of functionalized ionic liquids (including dual-functionalized ionic liquids) have been highlighted in this review. Ionic liquids are at-tracting attention as alternative solvents in green chemistry, but as more functionalized ILs are pre-pared, a greater number of applications in increasingly diverse fields are found.

关键词： ionic liquids functionalized ionic liquids dual-functionallzed ionicliquids reaction media asymmetric synthesis nano-materials porous materials lubricants flue-gas desulfurization oil desul-furization

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Structural components of the nuclear body in nuclei of Allium cepa cells

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Cell Research 2001年第1期11卷 68-73页

作者： TAO WEI, CHANG HUI YAN, TAO CAI, SHUI HAO, ZHONG HE ZHAI (college of life Sciences, peking university, {3., china) (institute of Genetics and Cytology, Northeast Normal university, Changchun Jilin 13.024, china) 1college of life Sciences Peking University Beijing 100871 China 1college of life Sciences Peking University Beijing 100871 China 1college of life Sciences Peking University Beijing 100871 China 2institute of Genetics and Cytology Northeast Normal University Changchun Jilin 130024 China 1college of life Sciences Peking University Beijing 100871 China

Nuclear bodies have long been noted in interphase nuclei of plant cells, but their structural component, origin and fUnction are still unclear by now. The present work showed in onion cells the nuclear bodies appeared as a spherical structure about 0.3.to 0.8 μm in diameter. They possibly were formed in nucleolus and subsequently released, and entered into nucleoplasm. Observation through cytochemical staining method at the ultrastructural level codeined that nuclear bodies consisted of ribonucleoproteins (RNPs) and silver-stainable proteins. Immunocytochemical results revealed that nuclear bodies contained no DNA and ribosomal gene transcription factor (UBF). Based on these data, we suggested that nuclear bodies are not related to the ribosome or other gene transcription activities, instead they may act as subnuclear structures for RNPs transport from nucleolus to cytoplasm, and may also be involved in splicing of pre-mRNAs.

关键词： Nuclear body structural component ribonucleoproteins nucleolus Allium cepa.

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