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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Qi Chen~(1,2,*),Hongying Peng~(1,*),Li Lei~(1,2,*),Ying Zhang~(1,2),Haibin Kuang~(1,2),Yujing Cao~1,Qi-xian Shi~3, Tonghui Ma~4,Enkui Duan~1 1.State Key laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,china.2.Graduate School of Chinese Academy of Sciences,Beijing 100049,china.3.Unit of Reproductive Physiology,Zhcjiang Academy of Medical Sciences,Hangzhou 310013,china.4.central research laboratory,bethune second hospital of jilin university,265 ziqiang street,changchun 130041,china.

In the journey from the male to female reproductive tract,mammalian sperm experience a natural osmotic decrease(e.g.,in mouse,from～415 mOsm in the cauda epididymis to～310 mOsm in the uterine cavity).At one hand,the hypotonic stress upon ejaculation is beneficial for mouse sperm motility "start-up"(evolutionary trait from fish sperm);However,the hypotonic stress could also cause potential harms to sperm function by inducing un-wanted cell *** counteract this negative impact, mammalian sperm have acquired mechanisms to drive rapid transmembrane water movement for efficient cell volume *** physiological and pharmacological studies have shown that ion channel-controlled water influx/efflux is actively involved in the process of sperm volume regulation;however,no specific sperm proteins have been found responsible for this rapid *** this study,we demonstrated that aquaporin3(AQP3) is localized in sperm tail membrane of mice and humans. Aqp3-deficient sperm show normal motility activation in response to hypotonicity but display increased vulnerability to hypotonic cell swelling,characterized by increased tail bending after entering *** sperm defect is a result of impaired sperm volume regulation and progressive cell swelling in response to physiological hypotonic stress during male-female reproductive tract ***-lapse imaging revealed that the cell volume expansion begins at cytoplasmic droplet,forcing the tail to angulate and form a hairpin-like structure due to mechanical membrane *** tail deformation hampered sperm migration into oviduct,resulting in impaired fertilization and reduced male *** data revealed AQP3 as an essential membrane pathway for sperm regulatory volume decrease(RVD) upon physiological hypotonicity,balancing the "trade-off’ between hypotonic induced sperm motility and cell swelling, thereby optimizing postcopulatory sperm *** the similar expression pattern of AQP3

关键词： aquaporin-3(AQP3) sperm osmoadaptation regulatory volume decrease(RVD) mechanosensing

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Impact of Interleukin-28B Polymorphism on Response to Treatment in Chinese Hepatitis C Patients

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Chemical research in Chinese Universities 2012年第1期28卷 79-83页

作者： Lü Jian-nan1, WANG Song2, XU Yan1, ZHANG Man-hua1, SIQING Tunala1, JI Shang-wei1, JIAO Jian1, WANG Xu1 and WANG Jiang-bin1 1. china.Japan Union hospital of jilin university, changchun 130033, P. R. china.2. The second hospital of jilin university, changchun 130041, P. R. china china.Japan Union hospital of jilin university Changchun 130033 The second hospital of jilin university Changchun 130041

The candidate polymorphism rs12979860 of interleukin 28B（IL28B） gene was genotyped by means of polymerase chain reaction（PCR） amplification and direct PCR sequencing, and then the role of this single nucleotide polymorphism（SNP） in the response to the treatment of 172 Chinese patients with hepatitis C virus（HCV） chronic infection was analyzed. The results show that the frequencies of major homozygotes（CC） and heterozygotes（CT） of rs12979860 were 0.84 and 0.16, respectively, and the minor homozygotes（TT） wasn’t found in this cohort. A highly significant association was found between the CC genotype and sustained virological response（SVR） in HCV geno type 1 infected patients（P〈0.001） but not in HCV non-genotype 1 infected patients. In addition, a strong association was found between rs12979860 CC genotype and rapid virological response（RVR） in both HCV genotype 1 infected patients（P〈0.001） and non-genotype 1 infected patients（P〈0.001）. Taken together, these results indicate that IL28B polymorphism plays a key role in response to hepatitis C therapy in Chinese patients. Combine assessment of clinical predictors and the IL28B polymorphism may be beneficial to the individualized treatment decision in Asian chronic hepatitis C（CHC） patients.

关键词： Hepatitis C virus(HCV) Interleukin 28B(IL28B) Interleukin-28B Gene

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The primary research on mouse and cat infected with A/Tiger/Harbin/01/2003(H5N1)

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中国实验动物学报 2005年第S1期13卷 7-8页

作者： Xia-xianzhu1, Gao-yuwei1, Yang-songtao1, Zhou-xiaohuan1, Chang-suang2,Zhao-hongxia3,Duan-ming4,Yueyuhuan1 1.Military Veterinary Institute, Academy of Military Medical Science, changchun, china. 130062, 2.Farming and Veterinary Institute, jilin university, changchun, china.130062, 3.The First hospital ,jilin university, changchun, china.130061, 4.The second Institute, Academy of Military Medical Science, Beijing, china.100071

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绝经过渡期妇女睡眠质量与血清雌二醇、5-羟色胺的相关性研究

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实用妇产科杂志 2008年第1期24卷 30-32页

作者：吴飞郭锡永刘景英易立岩吉林大学第二医院吉林长春130041 吉林大学再生医学科学研究所吉林长春130021 吉林省妇幼保健院吉林长春130041

目的：探讨绝经过渡期妇女睡眠质量与血清雌二醇（E2）、5-羟色胺（5-HT）的相关性。方法：采用匹兹堡睡眠质量指数问卷（PSQI）对吉林大学第二医院妇女保健门诊40—55周岁的绝经过渡期妇女进行睡眠质量的评价；采用病例对照方法，应用... 详细信息

目的：探讨绝经过渡期妇女睡眠质量与血清雌二醇（E2）、5-羟色胺（5-HT）的相关性。方法：采用匹兹堡睡眠质量指数问卷（PSQI）对吉林大学第二医院妇女保健门诊40—55周岁的绝经过渡期妇女进行睡眠质量的评价；采用病例对照方法，应用放免法和荧光法对绝经过渡期妇女睡眠质量差组（I组）、绝经过渡期妇女睡眠质量正常组（Ⅱ组）和生育年龄正常对照组（Ⅲ组）妇女各30例进行血清E2、5-HT水平的测定。结果：Ⅰ组的血清E2水平和5-HT水平低于Ⅱ组和Ⅲ组，且差异有显著性（P〈0．05）；绝经过渡期组血清5-HT与E2水平呈正相关性（r=0．025，P〈0．01）。结论：绝经过渡期妇女睡眠质量差的发生与血清E2及5-HT水平降低有关。

关键词：绝经过渡期睡眠雌二醇 5-羟色胺

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Effects of Nanoβ-TCP/Gelatin/Velvet Antler Polypeptide Composites on Restoration of rabbit mandibular defects

Effects of Nanoβ-TCP/Gelatin/Velvet Antler Polypeptide Comp...

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第二届中国鹿业发展大会

作者： HU Jin-ping~1,LIU Xiao-feng~2,TIAN Xiu-Li~1,ZHANG Jin~1,QUAN Long-quan~1,SHI Qi-Yun~1, HE Zhong-Mei~3,ZHOU Qiu-Li~4,DENG Xu-ming~1,SONG Yu~(1,*) 1.College of Animal Science and Veterinary Medicine,jilin university,changchun,china. 2.Department of Clinical laboratory,jilin Province Tumor hospital,changchun,china. 3.jilin Agricultural university,changchun,china. 4.School of pharmaceutical sciences,jilin university,changchun,china.

This study was designed to evaluate the effects of Nanoβ-TCP/Gelatin/Velvet Antler Polypeptide (Nanoβ-TCP/Gelatin/VAP) composites on reparation of rabbit mandible *** this study, morphological and histological evaluations were carried out using 80 consecutively treated *** animals were divided into four groups:the first group was material group containing 5mg/g of VAP;the second group was material group containing 20mg/g of VAP;the third was control group which was no VAP

关键词： Nanoβ-TCP/Gelatin/Velvet Antler Polypeptide Rabbit mandibular defect Osteoid New bone formation

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围绝经期及绝经后妇女睡眠质量影响因素研究

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国外医学（妇幼保健分册） 2005年第5期16卷 312-315页

作者：吴飞郭锡永吉林大学第二医院妇女卫生教研室吉林长春130041

睡眠质量是围绝经期及绝经后妇女健康状态及生活质量的重要决定因素,然而,睡眠质量受多种因素的影响,该文就性激素、躯体症状、5-羟色胺、社会心理因素、绝经状态与围绝经期及绝经后女性睡眠质量的相关性研究作以综述。

关键词：围绝经期绝经睡眠质量影响因素

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端粒及端粒酶与颈癌及癌前病变的关系

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国外医学（妇幼保健分册） 2003年第6期14卷 396-399页

作者：万俊崔满华吉林大学第二医院吉林长春130021

近年来 ,有关端粒和端粒酶及其与肿瘤之间关系的研究已成为各科研究的热点 ,认为人类端粒酶与恶性肿瘤的发生和发展有密切的关系 ,研究已取得了长足的进展。该文就端粒酶在宫颈癌及癌前病变方面的研究、进展情况及端粒酶活性与宫颈癌的... 详细信息

近年来 ,有关端粒和端粒酶及其与肿瘤之间关系的研究已成为各科研究的热点 ,认为人类端粒酶与恶性肿瘤的发生和发展有密切的关系 ,研究已取得了长足的进展。该文就端粒酶在宫颈癌及癌前病变方面的研究、进展情况及端粒酶活性与宫颈癌的诊断及预后的关系进行了综述。

关键词：端粒端粒酶官颈癌癌前病变

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Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation

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Cell research 2005年第7期15卷 539-547页

作者： Peihsien TANG Department of Cell Biology Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100850 china

ABSTRACTHuman placenta-derived mononuclear cells (MNC) were isolated by a Percoll density gradient and cultured in mesen-chymal stem cell (MSC) maintenance medium. The homogenous layer of adherent cells exhibited a typical fibroblast-like morphology, a large expansive potential, and cell cycle characteristics including a subset of quiescent cells. In vitrodifferentiation assays showed the tripotential differentiation capacity of these cells toward adipogenic, osteogenic andchondrogenic lineages. Flow cytometry analyses and immunocytochemistry stain showed that placental MSC was ahomogeneous cell population devoid of hematopoietic cells, which uniformly expressed CD29, CD44, CD73, CD105,CD166, laminin, fibronectin and vimentin while being negative for expression of CD31, CD34, CD45 and α-smoothmuscle actin. Most importantly, immuno-phenotypic analyses demonstrated that these cells expressed class I majorhistocompatibility complex (MHC-I), but they did not express MHC-II molecules. Additionally these cells could sup-press umbilical cord blood (UCB) lymphocytes proliferation induced by cellular or nonspecific mitogenic stimuli. Thisstrongly implies that they may have potential application in allograft transplantation. Since placenta and UCB arehomogeneous, the MSC derived from human placenta can be transplanted combined with hematopoietic stem cells(HSC) from UCB to reduce the potential graft -versus-host disease (GVHD) in recipients.

关键词： mesenchymal stem cells human placenta umbilical cord blood immune regulation.

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