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Identification and Characterization of a Rat Novel Gene RSEP4.Expressed Specifically in Central Nervous System

Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4. and Nai-He JING1* 1 Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, institute of Biochemistry and Cell Biology, shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, shanghai 200031,{4. School of Life Science and Biotechnology, 2 shanghai Jiaotong University, shanghai 200030,{4. institute of Neurobiology, Fudan University, shanghai 2004.3,{4. 3 4. Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA Laboratory of Molecular Cell Biology and Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4.gene (RSEP4.. The total length of RSEP4.cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4.was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4.was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4.EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4.protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4.may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

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General gambogic acids inhibited growth of human hepatoma SMMC-7721 cells in vitro and in nude mice

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Acta Pharmacologica Sinica 2004年第6期25卷 67-72页

作者： Qing-long GUO2, Qi-dong YOU3, Zhao-qiu WU, Sheng-tao YUAN4.Li ZHAO Department of Physiology, Department of Medicinal Chemistry, china Pharmaceutical University, Nanjing 210009 3 4.1} of Materia Medica, shanghai institutes for Biological Sciences, ChineseAcademy ofSciences, shanghai 200031, china Department of Physiology Department of Medicinal Chemistry China Pharmaceutical University institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

AIM: To study the inhibitory effect of general gambogic acids (GGA) on transplantation tumor SMMC-7721 in experimental animal model and SMMC-7721 cells in vitro. METHODS: Anti-tumor activity of GGA in the experi- mental transplantation tumor SMMC-7721 was evaluated by relative tumor growth ratio. Cell morphology was observed with inverted microscope and electron microscope. Cell proliferation was measured by MTT assay and the telomerase activity was determined by PCR. RESULTS: In vivo study indicated that GGA (2, 4. and 8 mg/kg, iv, 3 times per week for 3 weeks) displayed an inhibitory effect on the growth of transplantation tumor SMMC- 7721 in nude mice compared with the normal saline group (P<0.01). At the concentrations of 0.625-5.0 mg/L, GGA remarkably inhibited the proliferation of SMMC-7721 cells in vitro. GGA 2 mg/L dramatically changed morphology of SMMC-7721 cells and inhibited the telomerase activity in SMMC-7721 cells. CONCLUSION: GGA had inhibitory effect on the growth of SMMC-7721, which might be related to its inhibition of telomerase activity.

关键词： gamboge gambogic acid hepatoma SMMC-7721 cells telomerase nude mice

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Visualization of chromatin folding patterns in chicken erythrocytes by atomic force microscopy (AFM)

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Cell research 1997年第2期7卷 143-150页

作者： QIAN RUO LAN ZHENG XIA LIU MEI YUN ZHOU HEN YUE XIE CHU JIANG ZHI JIANG YAN(shanghai institute of Cell Biology, Chinese Academy ofSciences, shanghai 200031, china)LI MIN QIAN YI ZHANG JUN HU(shanghai institute of nuclear research, Chinese Academyof shanghai institute of nuclear research Chinese Academy of Sciences Shanghai 201800 China

The organization of the higher order structure of chromatin in chicken erythrocytes has been examined withtapping-mode scanning force microscopy under conditionsclose to their native environment. Reproducible highresolution AFM images of chromatin compaction at several levels can be demonstrated. An extended beads-on-astring (width of ～ 15-20nm, height of ～ 2-3nm for eachindividual nucleosome) can be consistently observed. Furthermore, superbeads (width of ～ 4.nm, height of ～ 7nm)are demonstrated. Visualization of the solenoid conformation at the level of 30nm chromatin fiber is attained eitherby using AFM or by using electron microscopy. In addition, tightly coiled chromatin fibers (～ 50-60nm and ～90-110nm) can be revealed. Our data suggest that the chromatin in the interphase nucleus of chicken erythrocyte represents a high-order conformation and AFM provides useful high-resolution structural information concerning thefolding pattern of interphase chromatin fibers.

关键词： The chromatin folding pattern chicken erythrocyte atomic force microscopy

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Passive Q-switching of Pulsed and CW Nd: YAG Lasers with Cr4.: YAG

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Chinese Journal of Lasers 1996年第3期B5卷 209-213页

作者： DING Yanhua OUYANG Bin WAN Xiaoke XU Jun LIN Lihuang(shanghai institute of Optics and Fine Mechanics, Academia Sinica,P. O. Box 800-211, shanghai 201800, china) shanghai Inst of Optics and Fine Mechanics Academia Sinica Shanghai China

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关键词： Passive Q-switch Cr￣(4.):YAG saturable absorber

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Dual Gabriel theorem with applications

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Science china Mathematics 2006年第1期49卷 9-26页

作者： CHEN Xiaowu, HUANG Hualin & ZHANG Pu Department of Mathematics, University of Science and Technology of china, Hefei 230026, china USTC shanghai institute for Advanced Studies, shanghai 201315, china Mathematical Section, the Abdus Salam ICTP, Strada Costiera 11, Trieste 34.14. Italy Department of Mathematics, shanghai Jiao Tong University, shanghai 200030, china 1. Department of Mathematics University of Science and Technology of China Hefei 230026 China 2. USTC shanghai institute for Advanced Studies Shanghai 201315 China 3. Mathematical Section the Abdus Salam ICTP Strada Costiera 11 Trieste 34014 Italy 4. Department of Mathematics Shanghai Jiao Tong University Shanghai 200030 China

We introduce the quiver of a bicomodule over a cosemisimple coalgebra. Applying this to the coradical C0 of an arbitrary coalgebra C, we give an alternative definition of the Gabriel quiver of C, and then show that it coincides with the known Ext quiver of C and the link quiver of C. The dual Gabriel theorem for a coalgebra with a separable coradical is obtained, which generalizes the corresponding result for a pointed coalgebra. We also give a new description of C1 = C0 ∧C C0 of any coalgebra C, which can be regarded as a generalization of the first part of the well-known Taft-Wilson Theorem for pointed coal-gebras. As applications, we give a characterization of locally finite coalgebras via their Gabriel quivers, and a property of the Gabriel quiver of a quasi-coFrobenius coalgebra.

关键词： quivers, cotensor coalgebra, quasi-coFrobenius coalgebra.

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Coherent transition radiation from REB in plasma ripple

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nuclear Science and Techniques 2000年第1期11卷 45-51页

作者： DAI Zhi-Min ZHANG Jin-Song ZHAO Xiao-Feng (National shanghai Synchrotron Radiation Center shanghai 201800 shanghai institute of {4. research, the Chinese Academy of Sciences, shanghai 201800 institute of Plasma Physics, the Chinese Academy of Scien shanghai institute of nuclear research the Chinese Academy of Sciences Shanghai 201800 National shanghai Synchrotron Radiation Center Shanghai 201800 institute of Plasma Physics the Chinese Academy of Sciences Hefei 230031

The coherent transition-radiation emission from an underdense rela tivistic beam of electrons, travelling through a dense plasma ripple, was studied. The evolution of this radiation field is described by a set of self-consistent pendulum-wave equations. Analytic calculations of the small-signal gain and numerical computations of the nonlinear saturation of this emission are presented. It is shown that such a device may provide a source of tunable coherent radiation ranging from the microwave to the infrared region.

关键词：等离子体自由电子激光器 REB 跃迁辐射相对论电子束

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A New Euphane-Type Triterpene from Melia Azedarach

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Chinese Chemical Letters 1999年第4期10卷 285-290页

作者： Wei Min ZHAO Cheng Qi FAN Zhi ZHANG (shanghai institute of Materia Medica. Academia Sinic4.294.Taiyuan Road, shanghai 200031) Acad Sinica Shanghai Inst Mat Med Shanghai 200031 Peoples R China

A 'ex'l Euphane-type triterpene. named azedarachic acid, was isolated from the fruits ofJab ha azedarach. Its structure was determined on the basis of NMR and MS experiments.

关键词： A4.lia ,4.edaroch triterpene azedarachic acid

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Jackknife and Bootstrap Tests of the Composition Vector Trees

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Genomics, Proteomics & Bioinformatics 2010年第4期8卷 262-267页

作者： Guanghong Zuo1,2, Zhao Xu1,3, Hongjie Yu1,4. and Bailin Hao1,5,6 1T-Life research Center & Department of Physics, Fudan University, shanghai 2004.3, china 2shanghai {4. of Applied Physics, Chinese Acadamy of Sciences, shanghai 201800, china 3Applied Biosystems, Inc., Beijing 100027, china 4.udan-VARI Center for Genetic Epidemiology, Fudan University, shanghai 2004.3, china 5{4. of Theoretical Physics, Chinese Acadamy of Sciences, Beijing 100190, china 6Santa Fe {4., Santa Fe, NM 87505, USA. [a] T-Life research Center & Department of Physics Fudan University shanghai 2004.3 china [b] shanghai institute of Applied Physics Chinese Acadamy of Sciences shanghai 201800 china [c] Applied Biosystems Inc. Beijing 100027 china [d] Fudan-VARI Center for Genetic Epidemiology Fudan University shanghai 2004.3 china [e] institute of Theoretical Physics Chinese Acadamy of Sciences Beijing 100190 china [f] Santa Fe institute Santa Fe NM 87505 USA

Composition vector trees （CVTrees） are inferred from whole-genome data by an alignment-free and parameter-free method. The agreement of these trees with the corresponding taxonomy provides an objective justification of the inferred phylogeny. In this work, we show the stability and self-consistency of CVTrees by performing bootstrap and jackknife re-sampling tests adapted to this alignment-free approach. Our ultimate goal is to advocate the viewpoint that time-consuming statistical re-sampling tests can be avoided at all in using this alignment-free approach. Agreement with taxonomy should be taken as a major criterion to estimate prokaryotic phylogenetic trees.

关键词： composition vector phylogeny topological distance bootstrap jackknife CVTree

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Surface Crystallized Glass with β-Barium Borate for Nonlinear Optical Applications

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Chinese Journal of Lasers 1997年第2期B6卷 189-192页

作者： ZHANG Qinyuan ZHAO Yonghua JIANG Zhonghong(shanghai institute of Optics & Fine Mechanics, Chinese Academy of SciencesP. O. Box 800-216, shanghai 201800) shanghai Inst of Optics and Fine mechanics Chinese Acad of Sciences Shanghai China

Optical second harmonic generation has been observed in surface crystallized but transpeent binary BaO-B2O3 and ternary BaO-B2O3-0. 1Al2O3 glasses using a Q -switched Nd:YAG laser. The only crystalline phase formed under controlled heat treatment of the base glass was β -BaB2O4.and preferred a -axis orientation of β -BaB2O4.fine grains was noticed. The glass surface crystallized layer is about 2μm in thickness and most grain size is less than 0. 3 μm.

关键词： glass-ceramics second harmonic generation surface crystallized

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SECONDARY HARDENING OF BAINITE IN HIGH SPEED STEEL

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Acta Metallurgica Sinica(English Letters) 1990年第2期3卷 99-103页

作者： YU Xuejie shanghai Iron and Steel research institute,shanghai,{4. ZHOU Tianjian Anhui institute of Technology,Hefei,{4. YU Xuejie Senior Engineer,shanghai Iron and Steel research institute,1001 Taihe Road,Wusong,shanghai 20094.,{4.

The mechanical properties and structure of bainite and martensite after tempering m 6-5-4.2 high speed steel have been *** was found that the secondary hardening effect and resistance to heat softening as well as impact toughness are higher in the bainite than in the *** observations show that the(Fe,M)_3C precipitated in the martensite after tempering at temperature near the peak of hardness-tempering temperature ***, no(Fe,M)_3C but the dispersive MC and M_2C precipitated in the ferrite and austenite of bainite after tempering at the same *** indicates that the diffusion of alloy ele- ments forming carbide might exist during the process of bainite growing.

关键词： high speed steel bainite secondary hardening

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