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Preparation of grain size controlled boron-doped diamond thin films and their applications in selective detection of glucose in basic solutions

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Science china Chemistry 2010年第6期53卷 1378-1384页

作者： ZHAO JianWen1,2,WANG JinLiang3,ZHI JinFang3 & ZHANG ZhiMing4.1College of Chemistry and Chemical engineering,Jishou university,Jishou 4.6000,china 2Schools of Materials Science and engineering,Nanyang Technological university,639798,Singapore 3Laboratory of Organic Optoelectronic Functional Materials and Molecular engineering,Technical Institute of Physics and Chemistry & Graduate university of Chinese Academy of Sciences,Beijing 100080,china 4.8} Institute of Micro-Nano Science and Technology,Shanghai Jiao Tong university,Shanghai 200030,china 1. College of Chemistry and Chemical engineering Jishou University Jishou 416000 China2. Schools of Materials Science and Engineering Nanyang Technological University 639798 Singapore Singapore3. Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering Technical Institute of Physics and Chemistry & Graduate University of Chinese Academy of Sciences Beijing 100080 China4. Research Institute of Micro-Nano Science and Technology Shanghai Jiao Tong University Shanghai 200030 China

Boron-doped diamond (BDD) thin films with different crystal grain sizes were prepared by controlling the reacting gas pressure using hot filament chemical vapor deposition (HFCVD).The morphologies and structures of the prepared diamond thin films were characterized by scanning electron microscopy (SEM) and Raman *** electrochemical responses of K4.e(CN)6 on different BDD electrodes were *** results suggested that electron transfer was faster at the boron-doped nanocrystalline diamond (BDND) thin film electrodes in comparison with that at other BDD thin film *** prepared BDD thin film electrodes without any modification were used to directly detect glucose in the basic *** results showed that the as-prepared BDD thin film electrodes exhibited good selectivity for detecting glucose in the presence of ascorbic acid (AA) and uric acid (UA).The higher sensitivity was observed on the BDND thin film grown on the boron-doped microcrystalline diamond (BDMD) thin film surface,and the linear response range,sensitivity and the low detection limit were 0.25–10 mM,189.1 μA mmol-1 cm-2 and 25 μM (S/N=3) for glucose in the presence of AA and UA,respectively.

关键词： diamond thin films electrochemical properties selective glucose detection

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Fusion Protein of Interleukin 4.and Diphtherial Toxin with High Cytotoxicity to Cancer Cells

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Acta biochimica et biophysica Sinica 2004年第6期36卷 437-442页

作者： Yu-Jian ZHANG, Xiao-BoHU, Su-Xia LI1, Li-Ping TIAN, Sheng-Li YANG, and Yi GONG* research center of biotechnology, Shanghai Institutes for biological Sciences,Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200233, {4. State Key Laboratory of bioreactor engineering, East {4. university of Science and Technology, Shanghai 200237, {4. research center of biotechnology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200233 China State Key Laboratory of bioreactor engineering East China University of Science and TechnologyShanghai 200237 China

Receptor of human interleukin 4.(hIL4.) has been found to be present on many types of cancer, so it may be a good target for cancer therapy. Here, fusion toxin gene DT4. has been constructed by fusing DNA sequence encoding the first 389 amino acids of diphtherial toxin (DT), which can not bind its own receptor, to human interleukin 4.(hIL4. gene. In order to improve the affinity of fusion toxin for hIL4., a circularly permuted form of hIL4.(cpIL4. was used. The fusion gene was expressed in Escherichia coli where the fusion toxin DT4. was highly expressed. Purified DT4. was very cytotoxic to cancer cell line U251 cells, and moderate cytotoxic to HepG2 and MCF-7 cells. SGC-7901 cells were insensitive to it. The cytotoxic action of DT4. was specific because it was blocked by excess hIL4. These results suggest that DT4. may be a useful agent in the treatment of certain malignancies.

关键词： fusion toxin diphtherial toxin cancer therapy interleukin 4 IL-4.receptor

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Enhancing disease resistances of Super Hybrid Rice with four antifungal genes

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Science china(Life Sciences) 2007年第1期50卷 31-39页

作者： ZHU HuaChen1, XU XinPing1, XIAO GuoYing2,3, YUAN LongPing2 & LI BaoJian1 1 biotechnology research center, Key Laboratory of Gene engineering of Ministry of Education, Sun Yat-sen {4., Guangzhou 510275, china 2 china National Hybrid Rice R&D center, hunan Hybrid Rice research center, changsha 4.0125, china 3 Institute of Subtropical Agriculture, the Chinese Academy of Sciences, changsha 4.0125, china 1. biotechnology research center Key Laboratory of Gene Engineering of Ministry of Education Sun Yat-sen University Guangzhou 510275 China 2. china National Hybrid Rice R&D center Hunan Hybrid Rice Research Center Changsha 410125 China 3. Institute of Subtropical Agriculture the Chinese Academy of Sciences Changsha 410125 China

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64., showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).

关键词： Super Hybrid Rice multi-gene transformation rice blast rice false smut rice kernel smut anti-fungi disease resistance

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Progress in study on endocrine disrupting pesticides (EDPs) in aquatic environment

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Science Bulletin 2005年第20期 3-12页

作者： XUE Nandong1, 2, WANG Hongbo2 & XU Xiaobai1 1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, research center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, china 2. Department of Environmental Sciences and engineering, {4. Ag- ricultural university, changsha 4.0128, china

Background on the generation of the problems of endocrine disrupting pesticides (EDPs) in aquatic environment, characteristics of EDPs, adverse effects and their effect mechanism of EDPs on human and wildlife, the transportation and degradation pathways of EDPs in water and analysis methods of EDPs in water were reviewed. The importance of EDPs in water should be attached to adverse effects on wildlife and human health. It was advised to estab- lish research programs on EDPs in aquatic environment es- pecially in water supply source.

关键词： endocrine disrupting pesticides (EDPs) aquatic envi- ronment.

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Anomalous magnetotransport in LaMn_(1-x)Te_xO_3

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Science china(Physics,Mechanics & Astronomy) 2009年第7期52卷 987-992页

作者： TAN GuoTai1,2, CHEN ZhengHao3 & ZHANG XiaoZhong4.5 1 Key Laboratory of Beam Technology & Materials Modification of the Ministry of Education, Institute of Low Energy Nuclear Physics, Beijing Normal university, Beijing 100875, china 2 Beijing Radiation center, Beijing 100875, china 3 Laboratory of Optical Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, china 4.Key Laboratory of Advanced Materials of the Ministry of Education, Department of Materials Science and engineering, Tsinghua university, Beijing 100084. china 5 Beijing National center for Electron Microscopy, Tsinghua university, Beijing 100084. china 1. Key Laboratory of Beam Technology & Materials Modification of the Ministry of Education Institute of Low Energy Nuclear Physics Beijing Normal University Beijing 100875 China 2. Beijing Radiation center Beijing 100875 China 3. Laboratory of Optical Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China 4. Key Laboratory of Advanced Materials of the Ministry of Education Department of Materials Science and Engineering Tsinghua University Beijing 100084 China 5. Beijing National center for Electron Microscopy Tsinghua University Beijing 100084 China

The LaMn1-xTexO3+δ (x=0.1, 0.2, 0.3) were synthesized using solid-state reaction method for the first time. X-ray photoemission spectrum (XPS) shows that in the samples the Te ions have a valence of Te4., and Mn3+ ions are partly converted into Mn2+ and Mn4. due to the excess oxygen and Te doping. The magnetotransport associated with Mn2+, Mn3+ and Mn4. was investigated. The experimental results show that the samples are insulator at 0 T when the amount of Mn3+ is much larger or less than the sum of Mn2+ and Mn4.; by contrast, the samples display metal to insulator transition with increasing temperature when the amount of Mn3+ is close to the sum of Mn2+ and Mn4.. These anomalous magnetotransport behaviors were analyzed in the frame of the double-exchange (DE) mechanism.

关键词： colossal magnetoresistance La-Te-Mn-O magnetotransport

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Mesh theory of angle modified dual tori double-enveloping toroidal worm drive

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Science china(Technological Sciences) 2010年第7期53卷 1913-1927页

作者： ZHAO YaPing1, SU DaiZhong2, ZHANG Zhao1, WEI WenJun3 & DONG XueZhu3 1 College of Machinery and Automation, Wuhan university of Science and Technology, Wuhan 4.0081, china 2 Advanced Design and Manufacturing engineering center, Nottingham Trent university, Nottingham NG1 4.U, UK 3 College of engineering, china Agricultural university, Beijing 100083, china 1. College of Machinery and Automation Wuhan University of Science and Technology Wuhan 430081 China2. Advanced Design and Manufacturing Engineering Center Nottingham Trent University Nottingham NG1 4BU UK3. College of Engineering China Agricultural University Beijing 100083 China

In this paper, the meshing theory of the angle modified hourglass worm drive is enriched and developed. The ordinary condition of the angle modification is derived and the physical significance of the modification is interpreted. A normal section methodology is proposed for meshing analysis, which can be used to compute the normal distance near a singular meshing point of a conjugate surface couple. By means of the method and after analyzing the normal transversals, it is specified that the worm helicoid, the nominal former contact zone and the new contact zone intersect each other along the locus of singular points of the instantaneous contact lines of an angle-modified worm pair. As a result, it is explained clearly that those three osculate each other but the osculations are different in degree. Moreover, the mechanism of removing the twice-contacted zone from the worm gear tooth surface is clarified and the reason of shortening the worm working length is also elucidated. With the help of the theory described in the present paper and the thorough and systematic research on the relevant meshing characteristics, the angle modified dual tori double-enveloping toroidal worm drive has been shown to be an excellent new-fashioned hourglass worm set.

关键词： hourglass worm angle modification double-enveloping osculation normal transversal

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GC-MS分析不同产地香荚兰商品荚乙醇提取物化学成分差异及其香草醛的测定

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中草药 2013年第8期44卷 955-959页

作者：孙皓刘薇曾建国彭旋刘军鸽杜方麓湖南中医药大学药学院湖南长沙410007 湖南省中药提取工程研究中心湖南长沙410331 湖南农业大学国家中药材生产(湖南)技术中心湖南长沙410128

目的分析中国海南与巴布亚新几内亚所产香荚兰商品荚乙醇提取物中化学成分的差异并测定香草醛的量。方法对不同样品处理方法进行比较,采用GC-MS分析法,利用Agilent DB-5MS弹性石英毛细管柱(30 m×0.32 mm,0.25μm),在初始温度为60℃,保... 详细信息

目的分析中国海南与巴布亚新几内亚所产香荚兰商品荚乙醇提取物中化学成分的差异并测定香草醛的量。方法对不同样品处理方法进行比较,采用GC-MS分析法,利用Agilent DB-5MS弹性石英毛细管柱(30 m×0.32 mm,0.25μm),在初始温度为60℃,保持1 min,以10℃/min升至260℃,保持6 min,再以6℃/min升至290℃,保持8 min;载气为氦气的条件下,对比两产地乙醇提取物的差异。结果两产地所产香荚兰商品荚乙醇提取物中分别鉴定出34.32种化合物,其中共有化合物18种。香草醛在香荚兰商品荚中的相对质量分数较高,定量测定结果表明,在质量浓度0.04.1.10 mg/mL内与峰面积呈良好的线性关系(r=0.999 8,n=5);回收率为100.05%,RSD为1.88%。结论本方法能快速、简便、高效地检测出香荚兰商品荚乙醇提取物的化学成分,并且在香草醛的定量测定中具有稳定、可重复的特点。

关键词： GC-MS 香荚兰商品荚乙醇提取物香草醛定量测定

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Dynamics of risers for earthquake resistant designs

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Petroleum Science 2010年第2期7卷 273-282页

作者： Duan Menglan Wang Yi Yue Zhiyong Estefen Segen Yang Xiaogang School of Naval Architecture and Marine engineering Dalian University of Technology Dalian Liaoning 116024 China Offshore Oil/Gas research center China University of Petroleum Beijing 102249 China Department of Mechanics and engineering Peking University Beijing 100080 China COPPE Federal University of Rio de Janeiro EO.Box 68508 RJ 21945-970 Brazil Design Division CNOOC Engineering Tanggu Tianjin 300452 China

It is well known that no criterion about seismic design for risers is available, and relevant research has not been reported. A comprehensive study of riser dynamics during earthquakes is performed in this paper. A dynamic model for seismic analysis of risers is developed in accordance with the working environment of the risers and the influence of inertia force of the pipelines. The dynamic equations for the developed model are derived and resolved on the basis of the energy theory of beams. Numerical simulation for an engineering project in the Bohai Oil Field, china shows that the fundamental frequency of the riser plays the major role in the seismic responses, and for platforms in shallow water in Bohai Bay, the risers demonstrate a much lower stress response due to prominent differences between the riser frequency and the earthquake wave frequency. The presented model and its corresponding method for seismic analysis are practical and important for riser design resistant to earthquake waves.

关键词： Subsea pipeline riser seismic design dynamic response earthquake wave Hamilton theory

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A RE-VISIT TO THE FORMULATION OF THE FINITE ELEMENT METHOD INSPIRED BY MESHFREE TECHNIQUES

A RE-VISIT TO THE FORMULATION OF THE FINITE ELEMENT METHOD I...

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庆祝中国力学学会成立50周年暨中国力学学会学术大会’2007

作者： LIU GR~(*,+,1)) HAN X~(**,2)) *(center for ACES,Department of Mechanical engineering,National university of Singapore,9 engineering Drive 1,117576,Singapore) +(Singapore-MIT Alliance,E4.04.10,4.engineering 3,117576,Singapore) **(College of Mechanical and Automobile engineering,hunan university,changsha,410082.china)

The finite element method is the most popular computational method for solving mechanics problems in *** is very well developed,widely used and technically very ***,this does not mean that further development is *** t...

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Coenzyme Q10 protects human dopaminergic cell line SHSY-5Y against rotenone-induced apoptosis

Coenzyme Q10 protects human dopaminergic cell line SHSY-5Y a...

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中国药理学会临床药理学专业委员会会议暨第十次全国临床药理学学术会议

作者： DENG Sheng~1 ZHANG Wei~2 YIN Tao~1 TANG Ling~1 DENG Hao~3 1 Department of Pharmacy,Xiangya Hospital,Central South university,changsha 4.0008,hunan,china 2 pharmacogenetics {4. Institute,Institute of Clinical Pharmacology,Central South university,changsha 4.0078,hunan,china 3 Medical Experimental center,The Third Xiangya Hospital,Central South university,changsha 4.0013,hunan,china

AIM:To determine whether the use of an antioxidant re- agent coenzyme Q10 can protect human dopaminergic cell line SHSY-5Y against rotenone-induced apoptosis. METHODS:Cell viability was quantified by 3-(4.5-dim- ethyhhiazol-2-yl)-2,5-diphenyhetrazolium bromide(MTF) assay and the apoptosis induced by rotenone was observed by Hoechst 3334.,propidium iodide,and ealcein AM staining in SHSY-5Y cells. RESULTS:Rotenone,a commonly used natural pesti- cide,inhibitor of mitochondrial respiratory chain complex I,was shown to decrease cell viability and induce apopto- sis in SHSY-5Y *** with coenzyme Q10,the electron transporter in the mitochondrial respiratory chain, remarkably increased cell viability as well as significantly reduced the percentage of apoptotic SHSY5Y cells induced by rotenone. CONCLUSION:Coenzyme QI0 has a beneficial effect in protecting against rotenone-indueed apoptosis in SHSY-SY *** results support the hypothesis that coenzyme QI0 is a component of the antioxidant machinery that pro- tects cell membranes from oxidative damage and decreases *** evaluation is required to determine whether the neuroprotective action of coenzyme Q10 can be used for the prevention and therapy of neurodegenerative diseases.

关键词： Coenzyme Q10 rotenone cell viability apoptosis

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