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animal husbandry and Feed Science 2012年第2期4卷 60-61,71页

作者： LIN Biao-sheng 1,2 ,HE Yu-qin 1,2 ,LUO Jian 3,4.,YANG Xiao-yan 1,2 1. College of Life Science of longyan University,longyan 364.12,china 2. Key Laboratory of Fujian Universities Preventive veterinary {4. and Biotechnology,longyan 364.12,china 3. longyan Jinhe animal Feed Co. ,Ltd. ,longyan 3..000,china 4. the animal husband- ry,veterinary medicine and marine products bureau of longyan,longyan 3..000,china

[Objective]The aim was to study on effects of fermented forages with highly active bacteria on immunity and oxidation resistance of fin- ishing pigs,providing references for popularization and application of the forage. [Method]About 180 duroc-landrace-yorkshire swines at the same age were chosen and two groups were set. In control group,the pigs were fed with basic forages and antibiotics,and in test group,the pigs were fed with basic forages and 8% of forages with highly active bacteria. [Result] Compared with control group,immune globulins in test group im- proved significantly and IgG differed extremely on the 28th d （ P 〈0. 01） ; blocking rate of antibody against swine fever enhanced by 4.. 10% on the 28th d than that on the 21th d with significant differences （ P 〈0. 05） ; oxidation resistance in test group was significantly higher and T-AOC achieved an extreme level （ P 〈0. 01） . [Conclusion]Forages with highly active bacteria would promote immunity and oxidation resistance of finishing pigs, which is of great value in production.

关键词： Fermented forage Finishing pig Immunity Oxidation resistance

Procyanidins from grape seeds protect against phorbol ester-induced oxidative cellular and genotoxic damage

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Acta Pharmacologica Sinica 2004年第8期 109-115页

作者： Yin LU, Wan-zhou ZHAO, Zai CHANG, Wen-xing CHEN, Lin LIDepartment of Pharmacology, marine Drug Research Center, Nanjing University of Traditional Chinese medicine,Nanjing 210029 Model animal Research Center (MARC), Nanjing University, Nanjing 210093, china Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14.63, USA

AIM: To evaluate the inhibitory effects of Vitis vinifera procyanidins (PAs) on carcinogen-induced oxidative stress. METHODS: The single cell gel electrophoresis technique (comet assay) was employed to detect DNA damage induced by the carcinogen phorbol-12-myristate-13-acetate (PMA). The release of hydrogen peroxidase from polymorphonuclear leukocytes (PMNs) was assayed by the horseradish peroxidase-mediated oxidation of phenol red. The microplate assay was used to detect the presence of oxidative products by means of 2',7'-dichlorofluorescin diacetate (DCFH-DA). The superoxide dismutase (SOD) activity of liver mitochondria was assayed, based on the ability of SOD to inhibit the generation of superoxidate anions by the xanthine-xanthine oxidase system. The malondialdehyde (MDA) level was determined by the thiobarbituric acid (TBA) assay. RESULTS: DNA of NIH3T3 cells was significantly damaged after addition of PMA. The length of the comet tail was observed ,while in normal cells the comet tail could not b

关键词： procyanidin antioxidation reactive oxygen species

鱼病毒性神经坏死病病毒(VNNV)不同基因型鉴别方法的建立及在VNN检疫和监测中的应用

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水产学报 2004年第6期28卷 695-702页

作者：刘荭史秀杰高隆英何俊强江育林陈焕春华中农业大学动物医学院动物病毒室深圳出入境检验检疫局动植食中心水生动物病重点实验室华中农业大学动物医学院动物病毒室湖北武汉　4.0070深圳出入境检验检疫局动植食中心水生动物病重点实验室广东深圳　518001

从GenBank中查找出乙型野田村病毒组中海水鱼类各病毒的序列,并用Sequencher多重序列比较软件将其分到条纹神经坏死病毒(stripedjacknervousnecrosisvirus,SJNNV)组、条纹星鲽神经坏死病毒(barfinfloundernervousnecrosisvirus,BFNNV)组、红点石斑鱼神经坏死病毒(redspottedgroupernervousnecrosisvirus,RGNNV)组和虎斑东方神经坏死病毒(tigerpuffernervousnecrosisvirus,TPNNV)组4.基因型的组别中。用DNAsis序列比较软件比较同一基因型各基因序列之间的同源性,均在85%以上;不同基因型之间序列的同源性,均在66%以下。结合Premier引物设计软件和Sequencher序列多重比较软件,设计了4.引物,采用逆转录聚合酶链式反应(RT PCR)来鉴别这4.不同的基因型。对从深圳口岸进境的产地为台湾的海水鱼苗和广东、福建两省养殖的主要海水鱼类进行检疫和监测,结果在进境的海水鱼苗中检出有RGNNV基因型的VNNV,在福建和广东省养殖的石斑鱼成鱼和鱼苗的病鱼体内均检测到RGNNV基因型的VNNV,对上述扩增产物基因序列进行比较,相似性均在96.5%以上,推导出的氨基酸序列与玛拉巴石斑鱼神经坏死病毒(MNNV)序列相似性均为100%。

关键词：病毒性神经坏死病病毒基因型鉴别

Healthy aquaculture pattern and technology without environment pollution

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Healthy aquaculture pattern and technology without environme...

Contrasting on Hydrocarbon Generation Modeland Characteristics of Pyrolysis of Modern Aquatic Plant(Gloeocapsa) vs marineanimal(Mantis Shrimp)

2003水产科技论坛

作者： Wang Anli Miao Yutao (Institute of marine Biology,College of Life Science,South china Normal University,Guangzhou 510631 ,china)

During the past two decades, aquaculture production has made impressive progress in many developing countries, where aquaculture has attained an economic and social importance. However, infectious diseases, high antibiotic level in some aquatic products cultured and water environmental pollution have seriously affected the profitability and the sustained development of their aquaculture industries. In view of the above mentioned facts, information is presented about the forming process of healthy aquaculture pattern without environmental pollution, the formation of the technology, the developing tendency,and the important roles of these patterns in the prevention and control of aquaculture diseases and the production of safe aquatic products in order to supply new ideas and methods for the sustained development of the world aquaculture industry.

关键词： aquatic animal aquaculture without environmental pollution healthy aqua-culture aquaculture pattern aquaculture technology

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Journal of Earth Science 2000年第1期19卷 30-38页

作者： Liu Dameng Department of Geology and Energy Resources, china University of Geosciences, Beijing 100083 Jin Kuili china University of Mining and Technology, Beijing 100083

A hydrocarbon model of the modern aquatic plant Gloeocapsa and the aquatic animal mantis shrimp was found in thermal simulating experiment. The results show that the modern aquatic plant Gloeocapsa is characterized by late generation, late termination and a long duration of oil generation, while the aquatic animal mantis shrimp is characterized by early generation, early termination and a short duration of oil generation. The nalkanes from Gloeocapsa and mantis shrimp are characterized by peak carbon C 15 -C 17 . With increasing thermal simulating temperature, the peak carbon changes from C 17 to C 15 , and the odd-even predominance of nalkanes becomes less clear. The products from Gloeocapsa contain abundant phenanthrene and naphthalene compounds, and even a little retene, while those from mantis shrimp are dominated by naphthalene compounds, and are poor in phenanthrene compounds. Gloeocapsa and mantis shrimp are rich in C 27 sterane, relatively rich in tricyclic terpanes (C 19 -C 29 ) and hopanes (C 27 -C 35 ), poor in 5α,14.,17β sterane, and coprostane does not disappear until 4.0 ℃. The tricyclic terpanes in Gloeocapsa are characterized by peak carbon C 23 and C 23 >C 21 , and in mantis shrimp by C 21 and C 21 >C 23 . The content of pregnanes is very low at low temperature of thermal simulation, while above 360 ℃, high levels of pregnane series compounds and even degraded C 25 and C 26 steranes occur in thermal simulation products.

关键词： Gloeocapsa mantis shrimp hydrocarbon generation thermal simulation molecular geochemistry.

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中国海绵天然产物的研究

天然产物研究与开发 2003年第4期15卷 359-363,368页

作者：薛松赵权宇张卫虞星炬金美芳袁权中国科学院大连化学物理研究所大连116023

据统计 ,从海洋生物的天然产物中获得药物或药物先导化合物的几率要比陆源生物高 ,海绵是海洋中除珊瑚以外的第二大生物资源。在海绵体内已发现许多高活性的化合物。中国海绵的研究主要集中在南海。南海海绵种类多、数量大 ,样品采集比... 详细信息

据统计 ,从海洋生物的天然产物中获得药物或药物先导化合物的几率要比陆源生物高 ,海绵是海洋中除珊瑚以外的第二大生物资源。在海绵体内已发现许多高活性的化合物。中国海绵的研究主要集中在南海。南海海绵种类多、数量大 ,样品采集比较容易。本文统计调查了国内外海绵研究的现状 ,重点综述中国已研究的海绵种类、所获得的天然化合物以及相关产物的药理研究结果 ,对上述研究的特点与存在的不足进行了分析、讨论。

关键词：中国海绵天然产物活性药理

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