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Exploration of Multifocal Rod Electroretinograms Recording in Human

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眼科学报 2002年第3期18卷 136-142页

作者： Changzheng Chen, Lezheng Wu, De-Zheng Wu, Shixian Long Jiongji Liang, Futian Jiang, Libing Jiangzhongshan Ophthalmic center, Sun Yat-sen {2., guangzhou 510060, China zhongshan Ophthalmic center Sun Yat-sen University Guangzhou 510060 China

Purpose:To test the feasibility of recording rod multifocal electroretinograms (ERG) in humans and observe appropriate recording ***: Multifocal rod ERG were recorded using a stimulus array of 61 equalsized hexagons in two normal subjects after the dark adaptation. Flashes were blue (W4.B). Blank frames between two successive flashes of hexagons varied from 0 to 14. Length of the m-sequence, bandwidth, flash frequency, flash intensities and background intensities were changed to obtain appropriate recording conditions for the clinical ***:Multifocal rod ERG were clearly recordable and well formed. They had an early implicit time, very small negative wave and a late implicit time, large positive wave. The positive wave was bimodal, whose timing and waveform were similar to the full-field rod ERG. The local response amplitudes can be suppressed with increase in flash frequency or background intensity, decrease in flash intensity or the size of stimulus ***: Multifocal rod ERG can be recorded in human and can provide topographical maps of retinal function that have clinical usage. 2.2.1 m-sequence length, 3F blank frames and 3～ 300 Hz bandwidth were suggested to appropriate recording conditions.

关键词：多源杆视网膜电图人体记录方法

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人颗粒细胞线粒体DNA缺失的研究

华中科技大学学报（医学版） 2004年第5期33卷 556-558页

作者：黄睿方丛李涛庄广伦广东妇幼保健院集爱遗传与不育诊疗中心广州510010 中山大学附属第一医院生殖中心广州510089

目的　探讨颗粒细胞线粒体DNA突变在女性生殖老化中的可能机制 ,并为颗粒细胞线粒体移植提供理论基础。方法　分离 70例临床行常规体外受精 (IVF)和胞质内单精子注射 (ICSI)患者的颗粒细胞 ,抽提DNA ,PCR检测颗粒细胞线粒体DNA (mitocho... 详细信息

目的　探讨颗粒细胞线粒体DNA突变在女性生殖老化中的可能机制 ,并为颗粒细胞线粒体移植提供理论基础。方法　分离 70例临床行常规体外受精 (IVF)和胞质内单精子注射 (ICSI)患者的颗粒细胞 ,抽提DNA ,PCR检测颗粒细胞线粒体DNA (mitochondrialDNA ,mtDNA) 4.977bp缺失情况。结果　所有颗粒细胞均未检测到mtDNA4.977bp缺失。结论　人颗粒细胞mtDNA并不发生 4.977bp缺失 ,这可能与其不是有丝分裂后细胞 ,仍具有增殖能力 ,受到的氧化损伤较少有关。颗粒细胞参与生殖老化的机制可能与其mtDNA突变无关。

关键词：颗粒细胞线粒体DNA 单精子注射 ICSI IVF 患者氧化损伤生殖 mtDNA突变有丝分裂

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筋膜囊下浸润麻醉与内眼手术

眼科学报 1999年第4期15卷 265-266页

作者：鲍光全中山医科大学中山眼科中心广州510060

目的:评价筋膜下浸润麻醉在内眼手术中的效果。方法:对白内障囊外摘出联合后房型人工晶体植入术14.,白内障抽吸联合后房型人工晶体植入术5眼,抗青光眼周边虹膜切除术2.,小梁切除术3眼,视网膜硅胶垫压术后取出硅胶带1眼,共2.眼,作筋膜... 详细信息

目的:评价筋膜下浸润麻醉在内眼手术中的效果。方法:对白内障囊外摘出联合后房型人工晶体植入术14.,白内障抽吸联合后房型人工晶体植入术5眼,抗青光眼周边虹膜切除术2.,小梁切除术3眼,视网膜硅胶垫压术后取出硅胶带1眼,共2.眼,作筋膜下浸润麻醉,方法是:在结膜囊内滴0.5％Dicain表面麻醉后,在角膜缘结膜作切口,用冲洗针头沿巩膜表面伸入10～15mm,向1O:00时及2.00时方位各注射0.75％布比卡因和2.利多卡因混合液1.5～2.l。结果:麻醉效果为:Ⅰ级2.眼(80％)Ⅱ级4.(16％)Ⅲ级1眼(4.)。结论:这种麻醉方法应用于内眼手术,其麻醉效果好,安全,简便。特别适合于独眼,晚期青光眼及年老体弱心功能不全的患者。眼科学报 1999;15:2.5-2.6.

关键词：筋膜囊下内眼手术浸润麻醉

大肠杆菌发酵生产重组人源抗-HBs Fab

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生物工程学报 2003年第1期19卷 87-91页

作者：安峰陈玉川范列英韩焕兴中山大学中山医学院法医病理学教研室广州510080 第二军医大学长征医院临床免疫中心上海200003

在KLF2.0 0 0发酵罐中利用补料分批培养技术培养表达含重组质粒pBAD HBsFab的TOP10大肠杆菌 ,生产人源抗 HBsFab ,为批量生产作准备。在发酵过程中 ,控制溶氧 30 %以上 ,温度 37℃ ,在基础培养基内生长 4.后 ,补加以甘油为碳源的补料 ,... 详细信息

在KLF2.0 0 0发酵罐中利用补料分批培养技术培养表达含重组质粒pBAD HBsFab的TOP10大肠杆菌 ,生产人源抗 HBsFab ,为批量生产作准备。在发酵过程中 ,控制溶氧 30 %以上 ,温度 37℃ ,在基础培养基内生长 4.后 ,补加以甘油为碳源的补料 ,继续生长到 9h ,加入阿拉伯糖 ,至终浓度为 0 0 2.% ,30℃诱导表达 5h ,收集菌体 ,纯化制备目的蛋白。利用Westernblot方法检测Fab抗原性 ,Dotblot方法检测生物学活性。 14.发酵结束后 ,菌体密度最终达 96g L ,纯化所得蛋白大约占菌体总蛋白的 6 % ,含量为 80mg L。以重组质粒pBAD HBsFab ,大肠杆菌TOP10表达生产的人源抗 HBsFab为可溶性具生物学活性的蛋白 ,与包涵体表达相比避免了变性、复性这一复杂过程 ,发酵罐表达比率与摇瓶相比没有降低 ,表达量达 80mg L左右 ,为大批量生产作了准备。

关键词：大肠杆菌发酵生产重组人源抗-HBs Fab 分批补料发酵培养人源化抗体

Growth Inhibition, Induction of Apoptosis by Green Tea Constituent (-)-Epigallocatechin-3-gallate in Cultured Rabbit Lens Epithelial Cells

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眼科学报 2000年第3期16卷 194-198页

作者： Wenyong Huang, Shaozhen Li, Junwen Zeng, Yizhi Liu, Mingxing Wu, Ming Zhangzhongshan Ophthalmic center, Sun Yat-sen {2. of Medical Sciences, guangzhou 510060, China zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To evaluate effect of green tea extract (-)-Epigallocatechin-3-gallate (EGCG) in cultured rabbit lens epithelial cells in order to pave a new way to postcapsular opacity (PCO) ***: Cell survival rate was measured by 3-(4. 5-dimethylthiazol-2.yl)-2. 5-diphenyl tetrazolium bromide (MTT) coloimetric assay. Cell apoptosis was detected by electron microscopy, Hochest 332.8 stain and flow cytometer. DNA fragment was detected using agarose gel ***: Proliferation of the cultured rabbit lens epithelia cells was inhibited by EGCG in a dose and time dependent manner. Morphologic study showed that the cells became shrunk, round shaped with their nuclei condensed and broken. Apoptotic bodies were also seen under electron microscope and in Hochest 332.8 stain assay 2.0}hours after EGCG was added to the medium. DNA ladders were shown in agarose gel eletrophoresis. In flow cytometry assay, apoptosis peak was also ***: Green Tea Constituent(-)

关键词：晶体上皮细胞生长抑制剂EGCG 上睑下垂

Comparison of the SchirmerⅠtest with and without topical anesthesia for diagnosing dry eye

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International Journal of Ophthalmology(English edition) 2012年第4期5卷 478-481页

作者： Na Li, Xin-Guo Deng, Mei-Feng He State Key Laboratory of Ophthalmology, zhongshan Ophthalmic center, Sun Yat-sen {2., guangzhou 510060, Guangdong Province, China

·AIM:To determine the value of Schirmer Ⅰtest (SⅠt) without anesthesia and with topical anesthesia for diagnosing dry eye (DE). ·METHODS:Totally 2.0 eyes in 110 patients, male (4.) and female (66), (39.56±12.67) years old diagnosed with DE were examined. SⅠt without anesthesia was performed firstly, and 15 minutes later, it was applied again in the same person after topical anesthesia with 0.5% proparacaine hydrochloride eye drops. The wetting strips counted <10mm per 5 minutes were defined positive, while ≤5mm per 5 minutes were defined strong positive. ·RESULTS:The wetting length in SⅠt after topical anesthesia was significantly lower than that in SⅠt without anesthesia(P < 0.001). The positive rate and strong positive rate of SⅠt after topical anesthesia were significantly higher than that of SⅠt without anesthesia (P <0.001). The positive rate and strong positive rate of SⅠt without anesthesia and the strong positive rate of SⅠt after topical anesthesia in patients with aqueous-deficiency dry eye (ADDE) were significantly higher than those in total patients whereas those in patients with evaporative dry eye (EDE) were significantly lower than those in total patients (P <0.001). ·CONCLUSION:SⅠt after topical anesthesia with 0.5% proparacaine hydrochloride eye drops is more objective and reliable than that without anesthesia in reflecting the status of DE, and its diagnostic value in patients with ADDE was even higher, making itself a meaningful evidence for the diagnosis and treatment of DE.

关键词： dry eye SchirmerⅠtest tears topical anesthesia

POINTS THAT MERIT CONSIDERATION IN THE DIAGNOSIS OF RETINOBLASTOMA

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Eye Science 1989年第Z1期14卷 3-6页

作者： Wenshu Mao Qiaoyun Ma Linghua Zeng Xianghong Lin Youzhao Chen Xinyuan Cao Zuyou Dai zhongshan Ophthahnic center Sun Yat-sen {2. of Medical Sciences guangzhou, China

According to the clinical appearances and the characteristics of the onset of 86 Rb cases, some points in the diagnosis merit consideration:1) Since the average symptomatic age was 2..75 months and the mean diagnostic age was 2.0}32.months, children under 3 years with leukocoria should be carefully examined, while adult Rb should not be neglected.2. The primary symptom of Rb is mostly leukocoria, but Rb eyes without any symptoms or leukocoria(2. out of 105 eyes in our groups) might also be encountered.3) 4.out of the 86 cases were retinocytoma which is supposed to be a type of Rb degeneration or *** Rh.4. Attention should be paid to abnormalities and second primary tumors in Rb patients.5) The pedigree investigations, twin method and laboratory studies in Rb such as chromosome analysis, EsD, LDH measurement and RFLP technique are useful in the prenatal diagnosis,early diagnosis and the diagnosis of the carriers.

关键词： carefully degeneration retinoblastoma chromosome neglected mostly supposed children diagnostic deletion

HUMORAL AND CELLULAR IMMUNE RESPONSES TO LENS PROTEIN IN PATIENTS WITH CONGENITAL CATARACT

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Eye Science 1989年第Z1期14卷 24-25页

作者： Chufang Xie Shaozhen Li Xiangkun Huang Wenshu Mao (Winifred Mao) Eye Research Institute, zhongshan Ophthalmic center Sun Yat-sen {2. of Medical Sciences guangzhou, China

In order to understand the immune responses to lens protein in patients with congenital cataract, the passive hemagglutination assay for humoral immunity and the leukocyte migration test for cellular immunity to lens protein were performed on 4. patients with congenital cataract and 30 healthy persons served as *** results showed that there was no significant difference in the incidence of humoral immune responses to lens protein between patients with congenital cataract and the controls (P>0.05), but the incidence of cellular immune responses tolensprotein in patients with congenital cataract was higher than that of the controls (P<0.005).The results of this study suggested that during the development of congenital cataract, lens protein leaked into the aqueous humour and entered the systemic circulation, sensitizing lymphocytes and producing cellular mediated immune responses. Further investigation is neceessary to determine whether there is any relationship between the immune responses to lens protein and the formation of congenital cataract.

关键词： cataract congenital immunity incidence leukocyte producing migration immunized served Ophthal

α-Al_2._3:Mn单晶的三维热释发光谱研究

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物理学报 2004年第11期53卷 3940-3944页

作者：张纯祥林理彬唐强罗达玲中山大学物理系广州510275 四川大学辐射物理及技术教育部重点实验室成都610064

测量了α Al2.O3:Mn单晶中子辐照前后的三维热释发光谱 .观察到α Al2.O3:Mn单晶γ射线照射后测量的三维热释发光谱中 ,峰温在 35 0℃波长为 6 80nm处有一宽发光峰 ,这可能与Mn2.+ 离子有关 ;波长为 6 95nm峰温在 170℃和35 0℃的线状光... 详细信息

测量了α Al2.O3:Mn单晶中子辐照前后的三维热释发光谱 .观察到α Al2.O3:Mn单晶γ射线照射后测量的三维热释发光谱中 ,峰温在 35 0℃波长为 6 80nm处有一宽发光峰 ,这可能与Mn2.+ 离子有关 ;波长为 6 95nm峰温在 170℃和35 0℃的线状光谱 ,叠加在 6 80nm宽发光峰上 ,是Cr3+ 离子的发光谱线 ,其中可能有Mn4.+ 离子的贡献 .与纯α Al2.O3单晶的热释发光谱相比 ,掺入Mn杂质后 ,γ射线照射的三维热释发光谱中完全地抑制了波长为 4.16nm的α Al2.O3的F心发光峰 .经 10 1 7cm- 2.中子注量辐照和退火后 ,γ射线照射后测量的三维热释发光谱中 ,在 15 0℃出现了波长为4.16和 6 95nm的发光峰 ,以及在 2.5 0℃波长为 6 80和 6 95nm的发光峰 ,其中 6 95nm新发光峰的强度略超过了中子辐照前α Al2.O3:Mn在 35 0℃波长为 6 95nm的发光峰 ,说明中子辐照产生了大量浅陷阱能级和F心 .然而 ,经 10 1 8cm- 2.中子注量辐照和退火后 ,γ射线照射后测量的三维热释发光谱中 ,出现了峰温 15 0℃ ,190℃和 2.5 0℃波长为 5 2.0nm的Mn2.+ 离子发光峰 ,以及 30 0℃波长为 6 80和 6 95nm的Cr3+ (或Mn4.+ )的发光峰 ,表明增高中子注量的辐照 ,产生了温度为 190℃ ,2.5 0℃和 30 0℃深陷阱能级和F心 ,并使Mn2.+

关键词：发光峰热释发光光谱研究波长离子 Cr^3+ 单晶中子辐照发光谱退火