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Comparative proteomic analysis of human osteosarcoma and SV4.-im-mortalized normal osteoblastic cell lines

Acta Pharmacologica Sinica 2007年第6期28卷 850-858页

作者： Qian-chen GUO~2.Jing-nan SHEN~(2.5),Song JIN~2.Jin WANG~2.Gang HUANG~2.Long-juan ZHANG~3,Jun-qiang YIN~2.Chang-yeZOU~2.Ming-tao LI~4.2.Department of Orthopedic Surgery,~3 Laboratory center of Surgery and ~4.proteomic center,Sun Yat-sen University,guangzhou 510080,china DepartmentofOrthopedicSurgery SunYat-senUniversityGuangzhou510080China LaboratorycenterofSurgery SunYat-senUniversityGuangzhou510080China Proteomiccenter SunYat-senUniversityGuangzhou510080China

Aim:Comparative proteomics provide a powerful approach in screening for alter-ations in protein levels and post-translational modifications that are associatedwith *** the present study,we aimed to identify candidate biomarkers todistinguish osteosarcoma (OS) cells from normal osteoblastic ***:Weemployed 3OS cell lines (U2.S,IOR/OS9,and SaOS-2.,and used the SV4.-immor-talized normal osteoblastic cell line (hFOB 1.19) as the *** differentialprotein levels in OS and osteoblastic cells were identified using 2.D gel electro-phoresis followed by matrix-assisted laser desorption/ionization-time of flightmass spectrometry *** proteins of interest,the levels of which weresignificantly increased in OS cells,were further characterized by Western ***:Twenty-six proteins were identified,the expression level ofwhich was either significantly increased or decreased in the OS cells as comparedto the control *** expression level of the activator of 90 kDa shock proteinATPase homolog 1 (AHA1),was enhanced 12.4.,2.0}1-,and 2..8-fold in SaOS-2.IOR/OS9,and U2.S cells,respectively,and the level of the stomatin-like protein 2.SLP-2. was increased by 10.4.and 7.8-fold in IOR/OS9 and U2.S cells,respectively,as compared to normal osteoblastic *** observations were confirmed byWestern blot ***:A differential proteomic analysis was suc-cessfully used to identify AHA 1 and SLP-2.that were significantly overproducedin OS cells as compared to normal osteoblastic cells,suggesting that those pro-teins among others may be effective biomarker candidates for the identification ofOS cells.

关键词： AHA1 differentially-produced protein osteosarcoma proteome SLP-2

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Activation of PI3-K/Akt pathway for thermal preconditioning to protect cultured cerebellar granule neurons against low potassium-induced apoptosis

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Acta Pharmacologica Sinica 2007年第2期28卷 173-179页

作者： Lin CAO~(2.3) De-xiong CAO~3 Xing-wen SU~2.Li-jun CHEN~2.Ai-lin LIU~2.Wei-jian JIAN~2.Yu-ping WU~2.Peng-xin QIU~2.Guang mei YAN~(2.4.2.Department of Pharmacology,zhongshan Medical School,Sun Yat-sen University,{2. 510080,china 3 Department of Anesthesiology The Second Affiliated Hospital,Sun Yat-sen University,{2. 51012.,china DepartmentofPharmacology ZhongshanMedicalSchoolSunYat-senUniversityGuangzhou510080China DepartmentofAnesthesiology TheSecondAffiliatedHospitalSunYat-senUniversityGuangzhou510120China

Aim:This study was designed to investigate whether the activation of thephosphatidylinositol 3-kinase(PI3-K)/Akt pathway is required for thermal pre-conditioning to protect rat cerebellar granule neurons(CGN)against apoptosisinduced by low potassium,and to explore the possibility of a link between theupregulated heat shock protein(HSP)70 expression and Akt activation in theacquisition of neuroprotection induced by thermal ***:CGN cultured for 8 d in vitro were switched to 5K medium for 2.0}h after thermalpreconditioning(TP;4..5 ℃ for 90 min,then 37 ℃ for 1 h).To study the role of thePI3-K/Akt pathway,a PI3-K inhibitor,LY2.4.02.2. μmol/L)was added into thecultures 1 h before TP.3-(4.5-dimethylthiazol-2.yl)2.5-diphenyltetrazolium bro-mide(MTT)assay and fluorescein diacetate staining were used to determine *** 332.8 staining and agar gel electrophoresis were used to testthe morphological and biological characters of *** blot analysis wasemployed to detect the levels of phospho-Akt,phospho-glycogen synthase ki-nase 3β(GSK3β) Akt,GSK3β,and ***:TP protected CGN againstapoptosis induced by low ***2.4.02.inhibited the neuroprotectiveeffect on CGN induced by *** induced a robust activation of Akt and theinactivation of GSK3β via ***,the activation of the PI3-K/Aktpathway by TP persisted for 2.0}h in the 5K ***2.4.02.2. μmol/L)failedto inhibit the upregulated HSP70 expression induced by ***:Theactivation of the PI3-K/Akt pathway is required for TP to protect CGN againstapoptosis induced by low potassium,but the neuroprotective effect by Akt acti-vation is not mediated through the downstream induction of HSP70 expression.

关键词： thermal preconditioning heat cerebellum neurons apoptosis LY2.4.02 phospho Akt phospho-GSK3β HSP70

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A Clinical Analysis of Idiopathic Orbital Inflammatory Pseudotumor

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眼科学报 2000年第3期16卷 208-213页

作者： Jianhua Yan, Zhongyao Wu, Yongping Lizhongshan Ophthalmic center, Sun Yat-sen University of Medical Sciences. {2. 510060 china zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To observe the clinical findings and response to treatment in patients with a diagnosis of idiopathic orbital inflammatory ***: 2.9 idiopathic orbital inflammatory pseudotumor cases seen between Jan 1, 1978 and Dec 31, 1999 in our hospital were evaluated retrospectively. Results: Of the 2.9 cases, 118 were male and 91 were female; there were 90 in the right eye, 81 left eye and 38 both eyes. Patients age ranged from 4.to 80 years (mean 4..4.. Proptosis (66%), palpable mass (65%), swollen eyelid (55%), increased orbital pressure (55%) and motility restriction (4.%) were the five most common presenting signs. According to radiologic and surgical findings, focal mass within orbit was the most frequent subtype (4.% ), followed by lacrimal inflammatory pseudotumor (32.), diffuse orbital inflammation (10%), myositis (8%) . Perineuritis (2.), periscleritis(2.), acute inflammation (2.) and eyelid pseudotumor (1%) were rare clinical findings. The response to treatment (with a mean

关键词：炎性假瘤眼眶肿瘤自发性临床分析

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悬移质近底参考面平衡浓度的一个计算公式——对Lane与Kalinske模式的改进

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水动力学研究与进展（A辑） 2004年第3期19卷 321-330页

作者：夏华永国家海洋局南海工程勘察中心

　根据推移质与床沙交换、悬移质主要与推移质交换的物理背景,采用Einstein床面泥沙的起动概念及推移运动概率函数表征床面泥沙起动及推移运动,采用Lane与Kalinske提出的泥沙因水流垂向脉动而进入悬浮运动的模式表征推移质进入悬浮运动... 详细信息

　根据推移质与床沙交换、悬移质主要与推移质交换的物理背景,采用Einstein床面泥沙的起动概念及推移运动概率函数表征床面泥沙起动及推移运动,采用Lane与Kalinske提出的泥沙因水流垂向脉动而进入悬浮运动的模式表征推移质进入悬浮运动的过程,建议一个床面层高度,建立了一个悬移质近底平衡浓度的一个计算公式。公式的参数由实测资料确定,公式经过了独立资料的检验,计算精度在可以接受的范围内。基于本文建议的床面层高度及参考面平衡浓度公式,建立了悬移质输沙率计算方法,取得了满意的输沙率计算精度。对于细颗粒泥沙的悬移质输沙率计算,本文公式克服了Einstein方法的缺陷。

关键词：床面层厚度悬移质近底平衡浓度输沙率

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CLINICAL STUDY OF LOW VISION PART Ⅰ: REFRACTION AND THE AIDED DISTANCE VISION

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Eye Science 1989年第Z1期14卷 47-51,43页

作者： Fu-shiang Chang De-Zheng Wu Lezheng Wu zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences {2., china

Eighty three cases of low vision patients (14. eyes) were examined with distant visual aids. The average visual acuity of the 14. eyes before refraction was approximately 0.07, while the counterpart afterwards was about 0.1 (p4. monocular focusable aid, acuity improved in 14. eyes out of the 14. eyes. The combined use of clip-on distance aid and the spectacle reading aid was observed to be especially suitable for partially sighted *** mean MEF of 14. eyes was 0.94.5±0.2.68. The paired T-test between the difference of actual magnification and theoretical magnification was not significant at the 5% level. The factors contributing to the variance of MEF were discussed.

关键词： acuity paired contributing counterpart refraction distant safely partially senile absolute

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Pioglitazone Ameliorate Lipid Metabolic Disorder in KKAy Mice

Pioglitazone Ameliorate Lipid Metabolic Disorder in KKAy Mic...

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广东省环境诱变剂学会、广东省预防医学会卫生毒理专业委员会2.10年学术会议

作者： Sifan Chen~(1,2.,Yanshuang Sun~(1,2.,Xincai Xiao~(1,2.3),Zili Zhang~(1,2.,Liangru Ke~4.Niman Zhou~4. Zhiming Shan~4.Xiang Feng~(1,2.),Wei Zhu~(3*) 1 Guangdong Provincial Key Laboratory of Food,Nutrition and Health 2.School of Public Health,Sun Yat-Sen University,guangzhou,Guangdong,P.R.china 3 guangzhou center for Disease Control and Prevention,guangzhou,Guangdong,P.R.china 4.zhongshan School of Medicine,Sun Yat-Sen University,guangzhou,Guangdong,P.R.china

To explore the effects of pioglitazone on serum and liver lipid level and antioxidant ability of mice with lipid metabolic disorder and the underlying mechanism,KKA mice were treated with pioglitazone based on their body weights for 12.weeks and their lipid and antioxidant indices were compared to those of KKA mice without pioglitazone treatment as well as those of C57BL/6J *** results show that pioglitazone treatment reduces serum and liver TG,elevates serum HDL-C level,increases serum and liver SOD activity,attenuates serum MDA content,ameliorates liver steatosis,induces liver PPARγexpression and enhances AMPKαphosphorylation level. In conclusion,the results indicate that pioglitazone can regulate blood lipid level,reduce liver lipid deposition and enhance antioxidant capacity of mice with lipid metabolic disorder possibly through increasing AMPK phosphorylation.

关键词： Pioglitazone lipid metabolic disorder AMP-activated protein kinase

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Changes in Local Immune Functions in Mooren's Ulcer

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Eye Science 1996年第1期16卷 33-35页

作者： Zheng Wang, Jiaqi Chen, Huling Zhengzhongshan Ophthalmic center, Sun Yat-sen Unicersity of Medical Sciences, {2. 510060,china zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

Purpose: To the investigate changes in local immune functions of the cornea and the adjacent conjunctiva, and their roles in the mechanism of the disease. Method: The cornea and adjacent bulbar conjunctiva taken from 14.patients with Mooren’s ulcer were stained immunohistochemically for CD3, CD4. CD8, HLA-DR, GD1 and ***: An aberrant expression of HLA-DR antigen by a large number of kerato-conjunctival epithelial cells and keratocytes in the corneal stroma was found. The CD4.+ /CD8+ ratio is significantly higher than normal control. Conclusion: The aberrant expression of MHC- II antigen in the resident cells at the peripheral cornea and the adjacent conjunctiva, along with a raised local TH/ Ts ratio leading to an excessive autoimmune reactivity is possibly the direct cause of Mooren’s ulcer. Eye Science 1996; 12. 33-35.

关键词： Mooren’s ulcer cornea antibody

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The Management of Secondary Glaucoma in Nanophthalmic Patients

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眼科学报 2002年第3期18卷 156-159页

作者： Shengsong Huang, Minbin Yu, Changyu Qiu, Tiancai Yezhongshan Ophthalmic center, Sun Yat-sen University, {2. 510060, china zhongshan Ophthalmic center Sun Yat-sen University Guangzhou 510060 China

Purpose: To investigate the clinical characteristcs, management of secondary glaucoma in nanophthalmos, and the prevention of its ***: Retrospectively, 9 cases (17 eyes) with nanophthalmic glaucoma were ***: The axial length of the eyes ranged (14.36 ～ 19.33) mm; All of the cases combined with hyperopia ranged (+7.00～+16.00)D. All 17 eyes had the manifestation like angle-closure *** glaucoma was controlled in 9 of 17 eyes at the early stage, which underwent laser iridotomy (4.of 9 eyes also underwent laser iridoplasty). 1 eye underwent ciliary photocoagulation because its visual acuity was lost and the patient complained of pain. The other 7 eyes underwent filtration surgery and 3 of them had permanent loss of vision caused by disastrous complications after the ***: Management of secondary glaucoma in nanophthalmos is complicated. The laser iris surgery is safe and effective in glaucoma at the early stage. Vortex vein decompression, sclerotectomy or anterior sclerotomy may be performed to reduce disastrous complications.

关键词：小眼畸形患者继发性青光眼临床管理

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Pterygium in Aged Population in Doumen County, china

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眼科学报 2002年第3期18卷 181-184页

作者： Kaili Wu, Mingguang He, Jingjing Xu, Shaozhen Lizhongshan Ophthalmic center, Sun Yat-sen University, {2. 510060, china zhongshan Ophthalmic center Sun Yat-sen University Guangzhou 510060 China

Purpose:To estimate the prevalence of pterygium and its influence on visual acuity in aged population of Doumen County,southern ***:A population-based survey was conducted in Doumen County,the rural area of southern china,twenty-seven sample units were selected by random *** prevalence and severity of pterygium and it-related low visual acuity were ***:Of the total 4.62.individuals aged 50 years or over,4.14.were *** prevalence of pterygium was 33.01% in subjects aged 50 years or *** was a significantly higher prevalence in female(35.70%)than in male(2..70%,P=0.02.).The prevalence of pterygium in age groups of 50s,60s,70s and 80s years old is 33.98%,36.4.%,30.73% and 2..4.%,*** of the pterygia were located in the nasal side(93.31%)of cornea,and only 6.69% in the temporal side.0.92. of the eyes had visual acuity less than 0.32.which were directly caused by ***:The current survey suggests that the pterygium is one of the most common diseases of external eye in th population ged 50 years and *** is still leading to the impairment of visual acuity in the rural area.

关键词：中国斗门县农村地区老年人群体翼状胬肉发生率

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THE PROPORTION OF VARIOUS TYPES OF CONGENITAL COLOR VISION DEFECTS

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Eye Science 1990年第Z1期15卷 40-42,19页

作者： Shizhou Huang Lezheng Wu De-zheng Wu zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences {2., china

One hundred and three color vision defective subjects were screened from 34.6 middle school students with pseudoisochromatic plate test One hundred subjects out of them were further examined with a test battery including Panel D—15 and FM 100—hue test and Neitz anomaloscope test It was found that there were 2. protanopes (P), 3 extremely protanomalous (EPA), 13 protanomalous (PA), 2. deutenopes (D), 5 extremely deuteranomalous (EDA), 2. deuteranomalous (DA), 6 unclassified subjects in our investigation. The proportion of various types of congenital color vision defects was P: EPA: PA: D: EDA: DA=1.00:0.14.0.62.1.19:0.2.0}1.33.

关键词：户口月人

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