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A family net of giant pandas in the tangjiahe natural {2.:Assessment of current individual migration

Science Bulletin 2005年第17期 65-72页

作者： WAN Qiuhong1, FANG Shengguo1, LI Jianguo2. ZHANG Liming 3, OU Weifu4. XIAN Fanghai4.& CHEN Wanli4.1. College of Life Sciences, State Conservation Center for Gene Re-sources of Endangered Wildlife, and Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals of Ministry of Education, Zhejiang University, Hangzhou 31002., China 2. Forestry Department of Sichuan Province, Chengdu 610081, China 3. Sichuan Bureau for Wildlife Resource Investigation, Conservation and Management, Chengdu 610082. China 4. tangjiahe natural reserve, qingchuan 62.109, China

In the present study, formalin-fixed feces, oli-gonucleotide fingerprinting and SRY-gene based sexing were used to construct a family net for giant pandas in the Tang-jiahe natural reserve and to assess contemporary gene flow (migration) in this population. A total of 12.2.fecal samples were attributed to 37 individuals (2. females and 15 males) that were then analyzed for family relationships. Based on DNA fingerprints, the deduced family net revealed the fol-lowing facts: (i) First-order relatives possessed similarities from 50% to 90%, and similarities between unrelated indi-viduals or distant relatives were as high as 77%, indicating that the {2. pandas are characterized by high genetic similarity; (ii) 15 matings were identified and 5 ones oc-curred between close relatives, implying that there is poten-tial for inbreeding to impact the pandas; (iii) four mating pairs and 5 offspring presented long distance migrations, demonstrating the intra-reserve habitat is continuous; (iv) four pairs of full sibs (also female-male dyads) dispersed short distance and all of them gave birth to highly inbred offspring, reflecting long distance migration is vital for in-breeding avoidance; (v) 17 adult individuals dispersed short or moderate distance and formed three clusters on the land-scape, indicating that it is necessary to find out whether there is a negative factor impacting the pandas.

关键词： multilocus DNA fingerprinting giant panda family rela-tionship close inbreeding dispersal pattern.

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Evolutionary genomics of climatic adaptation and resilience to climate change in alfalfa

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Molecular Plant 2024年第6期17卷 867-883页

作者： Fan Zhang Ruicai Long Zhiyao Ma Hua Xiao Xiaodong Xu Zhongjie Liu Chunxue Wei Yiwen Wang Yanling Peng Xuanwen Yang Xiaoya Shi Shuo Cao Mingna Li Ming Xu Fei He Xueqian Jiang Tiejun Zhang Zhen Wang Xianran Li Long-Xi Yu Junmei Kang Zhiwu Zhang Yongfeng Zhou qingchuan Yang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China National Key Laboratory of Tropical Crop Breeding Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China School of Grassland Science Beijing Forestry UniversityBeijing 100083China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Crop and Soil Sciences Washington State UniversityPullmanWA 99163USA U.S.Department of Agriculture-Agricultural Research Service Plant Germplasm Introduction and Testing ResearchProsserWA 99350USA National Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China

Given the escalating impact of climate change on agriculture and food security,gaining insights into the evolutionary dynamics of climatic adaptation and uncovering climate-adapted variation can empower the breeding of climate-resilient crops to face future climate ***(Medicago sativa ***),the queen of forages,shows remarkable adaptability across diverse global environments,making it an excellent model for investigating species responses to climate *** this study,we performed population genomic analyses using genome resequencing data from 702.accessions of 2.0}Medicago species to unravel alfalfa’s climatic adaptation and genetic susceptibility to future climate *** found that interspecific genetic exchange has contributed to the gene pool of alfalfa,particularly enriching defense and stress-response *** introgression between *** ***(***)and alfalfa not only aids alfalfa’s climatic adaptation but also introduces genetic burden.A total of 1671 genes were associated with climatic adaptation,and 5.7%of them were introgressions from *** integrating climate-associated variants and climate data,we identified populations that are vulnerable to future climate change,particularly in higher latitudes of the Northern *** findings serve as a clarion call for targeted conservation initiatives and breeding *** also identified preadaptive populations that demonstrate heightened resilience to climate fluctuations,illuminating a pathway for future breeding ***,this study enhances our understanding about the local adaptation mechanisms of alfalfa and facilitates the breeding of climate-resilient alfalfa cultivars,contributing to effective agricultural strategies for facing future climate change.

关键词： Medicago local adaptation population genetics adaptive introgression genetic vulnerability alfalfa breeding

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Precise 3D shape measurement of three-dimensional digital image correlation for complex surfaces

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Science China(Technological Sciences) 2018年第1期61卷 68-73页

作者： YAN TianHao SU Yong ZHANG qingchuan CAS Key Laboratory of Mechanical Behavior and Design of Materials Department of Modern Mechanics University of Science and Technology of China

Three dimensional-digital image correlation （3D-DIC） is a widely used optical metrology in the experimental mechanics community because of its reliability, practicality, and flexibility. Although the precision of digital image correlation （DIC） has been thoroughly studied theoretically and numerically, verification experiments have seldom been performed, especially fbr complex surfaces with a small field of view （FOV）. In this work, the shape of a 1-yuan coin was measured using 3D-DIC; the shape was complex due to the presence of many fine details, and the FOV was relatively small because the coin diameter was only 2. mm. During the experiment, a novel strategy for speckle production was developed： white paint was simply sprayed onto the surface. Black paint was not used; instead, taking advantage of the reflective nature of the coin surface, polarized light and a Polaroid filter were introduced, and the polarization direction was carefully adjusted, ensuring that the spray pattern was extremely thin and that high-quality speckle images with significant contrast were captured. The three-dimensional coin shape was also successfully determined for comparison using a stylus profiler. The results demonstrate that 3D-DIC provides high precision in shape measurement even for complex surfaces with small FOV. The precision of 3D-DIC can reach 1/7000 of the field of view, corresponding to about 6 ~tm in this experiment.

关键词： three-dimensional digital image correlation(3D-DIC) measurement precision complex surfaces small field of view polarized light polaroid filter

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Optimal Design of Multiple Stresses Accelerated Life Test Plan Based on Transforming the Multiple Stresses to Single Stress

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Chinese Journal of Mechanical Engineering 2014年第6期27卷 1125-1132页

作者： GAO Liang CHEN Wenhua QIAN Ping PAN Jun HE qingchuan College of Mechanical and Electrical Engineering Sichuan Agricultural University Zhejiang Province's Key Laboratory for Reliability Technology of Mechanical & Electrical Products Zhejiang Sci-Tech University

For planning optimum multiple stresses accelerated life test plans, a commonly followed guiding principle is that all parameters of the life-stress relationship should be estimated, and the number of the stress level combinations must be no less than the number of parameters of the life-stress relationship. However, the general objective of an accelerated life test（ALT） is to assess thep-th quantile of the product life distribution under normal stress. For this objective,estimating all model parameters is not necessary, and this will increase the cost of test. Based on the theoretical conclusion that the stress level combinations of the optimum multiple stresses ALT plan locate on a straight line through the origin of coordinate, it is proposed that a design idea of planning the optimum multiple stresses ALT plan through transforming the problem of designing an optimum multiple stresses ALT plan to designing an optimum single stress ALT plan. Moreover, a method of planning the optimum multiple stresses ALT plan which can avoid estimating all model parameters is established. An example shows that, the proposed plan which only has two stress level combinations could achieve an accuracy no less than the traditional plan, and save the test time and cost on one stress level combination at least; when the actual product life is less than the design value, even the deviation of the model initial parameters value is up to 2.%, the variance of the estimation of thep-th quantile of the proposed plan is still smaller than the traditional plans approximately 2.%. A design method is provided for planning the optimum multiple stresses ALT which uses the statistical optimum degenerate test plan as the optimum multiple stresses accelerated life test plan.

关键词： multiple stresses accelerated testing optimum design test planning

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Enhancing General Corrosion Resistance of Biomedical High Nitrogen Nickel-Free Stainless Steel by Nitric Acid Passivation

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Acta Metallurgica Sinica(English Letters) 2020年第2期33卷 307-312页

作者： Yixun Yang qingchuan Wang Jun Li Lili Tan Ke Yang Institute of Metal Research Chinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China

A systematic study of nitric acid passivation was investigated to enhance the general corrosion resistance of biomedical high-nitrogen nickel-free stainless steels(HNSs).After passivation,the corrosion rate of HNS could dramatically reduce to 1/2. of the untreated in 37℃0.9 wt% NaCl ***,the passive film on HNS was analyzed by X-ray photoelectron *** was found that chromium enrichment in the passive film and nitrogen enrichment in the film/metal interface contributed to the improvement in general corrosion resistance of HNS.

关键词： Stainless steel Nitric acid passivation General corrosion Passive film

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Task Coordination Organization Model and the Task Allocation Algorithm for Resource Contention of the Syncretic System

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Tsinghua Science and Technology 2016年第4期21卷 459-470页

作者： Danfeng Wu Guangping Zeng Di He Zhaopeng Qian qingchuan Zhang School of Computer&Communication Engineering University of Science&Technology BeijingBeijing 100083China School of Software Liaoning Technical UniversityHuludao 125105China Beijing Key Laboratory of Knowledge Engineering for Materials Science Beijing 100083China Graduate Center City University of New YorkNew YorkNY 10016USA

At present, robot embedded systems have some common problems such as closure and poor dynamic evolution. Aiming at resolving this situation, our paper focuses on improvements to the robot embedded system and sets up a new robot system architecture, and we propose a syncretic mechanism of a robot and SoftMan （SM）. In the syncretic system, the structural organization of the SoftMan group and its modes are particularly important in establishing the task coordination mechanism. This paper, therefore, proposes a coordination organization model based on the SoftMan group, and studies in detail the process of task allocation for resource contention, which facilitates a rational allocation of system resources. During our research, we introduced Resource Requirement Length Algorithm （RRLA） to calculate the resource requirements of the task and a resource conformity degree allocation algorithm of Resource Conformity Degree Algorithm （RCDA） for resource contention. Finally, a comparative evaluation of RCDA with five other frequently used task allocation algorithms shows that RCDA has higher success and accuracy rates with good stability and reliability.

关键词： SoftMan robot syncretic system organization model task allocation game theory

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Performance analysis of microcantilever array sensing

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Science China(Technological Sciences) 2017年第11期60卷 1674-1680页

作者： ZHOU XiaRong LIU Hong WU ShangQuan ZHANG qingchuan WU XiaoPing Key Laboratory of Mechanical Behavior and Design of Material Chinese Academy of SciencesDepartment of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Department of ChemicalPhysics University of Seienee and Technology of ChinaHefei 230026China

Array sensing is increasingly important in the development of microcantilever(MC) sensors, and response consistency is the foundation for MC array sensing. In the present work, we investigated the response consistency of MC array sensing. The responses of two types of commercially available MC arrays were studied under conditions of temperature change, solution replacement and biochemical molecular interaction. For the thermal response, the deflections of both arrays were found to be proportional to temperature, and the responses of the MCs in both arrays were consistent with each other. The thermal response sensitivity for each MC during temperature increase and decrease also showed good consistency. Moreover, the MC array showed good consistency for the response induced by solution replacement. Finally, we also demonstrated that the MC array had good consistency in biochemical detection, exemplified by aflatoxin antibody-anti gen binding. The good response consistency makes this technology reliable and accurate for biochemical sensing.

关键词： microcantilever array thermal response environmental disturbance biochemical detection

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AttentionSplice: An Interpretable Multi-Head Self-Attention Based Hybrid Deep Learning Model in Splice Site Prediction

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Chinese Journal of Electronics 2022年第5期31卷 870-887页

作者： YAN Wenjing ZHANG Baoyu ZUO Min ZHANG qingchuan WANG Hong MAO Da Division of Chemical Metrology and Analytical Science National Institute of Metrology National Engineering Laboratory for Agri-Product Quality Traceability Beijing Technology and Business University

Pre-mRNA splicing is an essential procedure for gene transcription. Through the cutting of introns and exons, the DNA sequence can be decoded into different proteins related to different biological *** cutting boundaries are defined by the donor and acceptor splice sites. Characterizing the nucleotides patterns in detecting splice sites is sophisticated and challenges the conventional methods. Recently, the deep learning frame has been introduced in predicting splice sites and exhibits high performance. It extracts high dimension features from the DNA sequence automatically rather than infers the splice sites with prior knowledge of the relationships, dependencies, and characteristics of nucleotides in the DNA sequence. This paper proposes the Attention Splice model, a hybrid construction combined with multi-head self-attention, convolutional neural network, bidirectional long short-term memory *** performance of Attention Splice is evaluated on the Homo sapiens(Human) and Caenorhabditis Elegans(Worm) datasets. Our model outperforms state-of-the-art models in the classification of splice sites. To provide interpretability of Attention Splice models, we extract important positions and key motifs which could be essential for splice site detection through the attention learned by the model. Our result could offer novel insights into the underlying biological roles and molecular mechanisms of gene expression.

关键词： Splice sites Multi-head self-attention Bioinformatics Deep-learning Long short-term memory (LSTM) Convolutional neural network (CNN)

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Investigation of Portevin–Le Chatelier Band Strain and Elastic Shrinkage in Al-Based Alloys Associated with Mg Contents

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Journal of Materials Science & Technology 2017年第6期33卷 580-586页

作者： Yulong Cai Suli Yang Shihua Fu Di Zhang qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Materials University of Science and Technology of China State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing

The Portevin–Le Chatelier（PLC） effect in Al–2.30wt%Mg, Al–4.57wt%Mg and Al–6.91wt%Mg alloys has been investigated at various applied strain rates at room temperature in this study. Three-dimensional digital image correlation（3D-DIC） technique was applied to obtaining the further insight into the spatiotemporal characteristics, in particular the influence of Mg content on deformation behaviors. Mg content has a pronounced effect on serration characteristics, including the serration type and amplitude; Mg content tends to weaken the spatial correlation of the propagative bands. Additionally, the serration amplitude linearly increases with the maximum PLC band strain; high Mg content generates a higher PLC band strain at a given serration amplitude compared with low Mg content. Mg content is found to be effective to enhance the serration amplitude, the maximum PLC band strain and also the amount of elastic shrinkage outside PLC bands.

关键词： Mg content Al–Mg alloy Portevin–Le Chatelier effect Digital image correlation Elastic shrinkage

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High strength and ductility achieved in friction stir processed Ni-Co based superalloy with fine grains and nanotwins

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Journal of Materials Science & Technology 2022年第11期106卷 162-172页

作者： Miao Wang Xingwei Huang Peng Xue Shangquan Wu Chuanyong Cui qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Materials University of Science and Technology of ChinaHefei 230027China Superalloy Division Institute of Metal ResearchChinese Academy of ScienceShenyang 110016China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of ScienceShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaHefei 230027China

The trade-off between strength and ductility has been an enormous difficulty in the field of materials for an extended time due to their inverse correlation. In this work, friction stir processing(FSP) was for the first time performed to high-strength and high-melting-point Ni-Co based superalloy(GH4.68),and enhanced strength and ductility were achieved in FSP samples. At room temperature, the FSP sample demonstrated significantly higher yield strength and ultimate tensile strength(12.0 and 1670 MPa)than that of the base material(BM, 758 and 904.MPa) and advanced wrought GH4.68 alloy(982.and 12.1 MPa), concurrent with high tensile ductility(~2.0}). Compared with the BM, 70% higher yield strength of the FSP sample results from the remarkable contribution of grain-boundary and nanotwin strengthening, which has been confirmed by the multimechanistic model studied in this work. More importantly, with increasing temperature, an excellent strength-ductility synergy was obtained at 4.0 ℃,i.e., the yield strength of the FSP sample was increased by more than 50% compared with the BM(from789 to 12.9 MPa);more interestingly, the elongation was also significantly increased from 17.9% in the BM to 2..5% in the FSP sample. Meanwhile, the Portevin-Le Chatelier effect was observed in the engineering stress-strain curve. The occurrence of this effect may be attributed to the interaction between solutes and defects like twins and mobile dislocations. Moreover, the grain refinement mechanism of FSP samples was proved to be discontinuous dynamic recrystallization.

关键词： Superalloy Friction stir processing Grain refinement Nanotwins

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