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食品与机械 2018年第7期34卷 169-172,203页

作者：吴波谢晶上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306 上海冷链装备性能与节能评价专业技术服务平台上海201306 上海201306

在分析鱼类运输应激反应的基础上,综合血液生化指标、非特异性免疫细胞、抗氧化功能等,从低氧胁迫、氨氮胁迫、密度胁迫、客观运输条件胁迫、温度胁迫等运输中可能产生不同胁迫的角度,详细综述鱼类保活运输中应激反应诱发因素及其影响。

关键词：鱼类保活运输应激反应因素影响

电池厂高频感应加热机辐射水平监测及干预研究

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实用预防医学 2011年第4期18卷 659-660页

作者：沙焱香映平谢子煌杨震宇何家禧深圳市职业病防治院广东深圳518001

目的监测电池厂高频感应加热机电磁辐射水平,评估干预措施的效果。方法用PMM8053B电场强度测量系统对4.电池厂高频加热机岗位进行检测。结果在无屏蔽状况下,所有岗位的电场强度均超过国家职业卫生标准(2. V/m)。屏蔽后分别在干预后3 d... 详细信息

目的监测电池厂高频感应加热机电磁辐射水平,评估干预措施的效果。方法用PMM8053B电场强度测量系统对4.电池厂高频加热机岗位进行检测。结果在无屏蔽状况下,所有岗位的电场强度均超过国家职业卫生标准(2. V/m)。屏蔽后分别在干预后3 d、干预后3个月、干预后6个月对相关岗位进行电场强度检测。干预后所有岗位的电场强度均未超过国家职业卫生标准。结论电池厂高频感应加热机作业员工的电场辐射暴露不容忽视,有效防护措施对于控制高频感应加热机的高频污染十分关键。

关键词：高频感应加热机电场强度干预

白细胞介素lra基因多态性与2.糖尿病关系的研究

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现代检验医学杂志 2004年第6期19卷 15-17页

作者：张健肖琴李卫宁北京大学深圳医院检验科广东深圳518036

目的探讨白细胞介素1受体拮抗剂(IL-lra)基因多态性与2.糖尿病的关系。方法应用聚合酶链反应技术对109例2.糖尿病患者和2.1例正常人的白细胞介素1受体拮抗剂基因(IL一1RN)数目可变的串联重复多态性进行分析。结果 2.糖尿病患者和... 详细信息

目的探讨白细胞介素1受体拮抗剂(IL-lra)基因多态性与2.糖尿病的关系。方法应用聚合酶链反应技术对109例2.糖尿病患者和2.1例正常人的白细胞介素1受体拮抗剂基因(IL一1RN)数目可变的串联重复多态性进行分析。结果 2.糖尿病患者和正常人IL-1RN Ⅰ／Ⅰ、Ⅰ／Ⅱ、Ⅱ／Ⅱ和Ⅰ／Ⅳ4.基因型频率分别为80.7％和81.1％,14.7％和16.9％,1.8％和0.5％,2.8％和1.5 9％;IL-1RN*Ⅰ等位基因频率分别为89.5％和90.3％,IL-1RN*Ⅱ等位基因频率分别为9.1％和9.0％。携带IL-1RN*Ⅱ等位基因的2.糖尿病组患者血糖和甘油三酯水平明显升高。结论 IL-1RN内含子2.因多态性与2.糖尿病易感性无关,但IL-1RN(Ⅱ)等位基因可能与2.糖尿病病情的危险性有关。

关键词：白细胞介素1 受体拮抗剂基因多态性 2.糖尿病

Allelic frequency distributions of 2. non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia,China

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 533-540页

作者： Hong-dan WANG Chun-mei SHEN Wen-juan LIU Yu-dang ZHANG Guang YANG Jiang-wei YAN Hai-xia QIN Bo-feng ZHU School of Medicine Xi'an Jiaotong University People's Hospital of Zhengzhou University Medical Genetic Institute of Henan Province People's Hospital of Henan Province blood center of Shaanxi Province Vascular Biology center Georgia Health Sciences UniversityAugustaGA 30912USA Beijing Institute of Genomics Chinese Academy of Science Department of Internal Medicine Morehouse School of MedicineAtlantaGA 30303USA

We studied the allelic frequency distributions and statistical forensic parameters of 2. new short tandem repeat(STR)loci and the amelogenin locus,which are not included in the combined DNA index system(CODIS),in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,China.A total of 114.bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction(PCR)*** capillary electrophoresis,the PCR products of the 2. STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.004. to *** 2. non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background,for individual identification,and for paternity testing in forensic practice.

关键词： Short tandem repeat(STR) Russian population Genetic polymorphisms

FG02.32.-loaded nanoparticle with PEG and PDLLA improved pharmacodynamics of reversing multidrug resistance in vitro and in vivo

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Acta Pharmacologica Sinica 2007年第6期28卷 913-920页

作者： Wen-jing DENG~2.Xiao-qiang YANG~2.Yong-ju LIANG~2.Li-ming CHEN~2.Yan-yan YAN~2.Xin-tao SHUAI~3,Li-wu FU~(2.4.~2.State Key Laboratory of Oncology in Southern China Cancer center,Sun Yat-Sen University,Guangzhou 510060,China ~3 School ofChemistry and Chemical Engineering of Sun Yat-Sen University,Guangzhou 5102.5,China StateKeyLaboratoryofOncologyinSouthernChinaCancercenter SunYat-SenUniversityGuangzhou510060China SchoolofChemistryandChemicalEngineeringofSunYat-SenUniversity Guangzhou510275China

Aim:FG02.32.,a novel imidazole derivative,is a potent multidrug-resistance(MDR) modulator in vitro and in ***,FG02.32. is ***-FG02.32. is a FG02.32.-1oaded nanoparticle formed with diblock copolymers ofpoly (ethylene glycol)-block-poly (D,L-lactic acid) (PEG:PDLLA,PEDLLA) thatcan solubilize *** work was intended to evaluate the pharmacody-namics of PEDLLA-FG02.32. on reversing MDR in vitro and in ***:Cytotoxicity was determined by tetrazolium *** intracellular accumulationand efflux of doxorubicin (Dox) were detected by fluorescence *** function of P-glycoprotein was examined by Rhodamine 12. (Rh12.) accumu-lation detected by flow *** KBv2.0 cell xenograft model was estab-lished to investigate the effect of PEDLLA-FG02.32. on reversing MDR in ***:PEDLLA-FG02.32. and FG02.32. exhibited 56.4.and 35.9-fold activityin reversing KBv2.0 cells to vincristine (VCR) resistance,respectively and 14.98-and 7.64.fold to Dox resistance,***-FG02.32. was much stron-ger than FG02.32.,resulting in the increase of Dox and Rh 12. accumulation andthe decrease of intracellular Dox extrusion in KBv2.0 ***,PEDLLA-FG02.32. exhibited more powerful activity than FG02.32. in enhancing the effectof VCR against KBv2.0 cell xenografts in nude mice,but did not appear more ***:The pharmacodynamics of FG02.32. was improved by incorporatingit into a micellar nanoparticle formed with PEG-block-PDLLA copolymers.

关键词： FG02.32. micellar nanoparticle multidrug resistance xenografts

活化α7乙酰胆碱受体促进LPS刺激的人牙髓干细胞牙/骨向分化

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南京医科大学学报（自然科学版） 2024年第2期44卷 145-153页

作者：李梦圆王宇萌徐青清关卓卞成玥江飞张光东南京医科大学附属口腔医院综合诊疗科江苏省口腔疾病研究重点实验室口腔医学转化研究中心江苏南京210029

目的:探讨活化α7乙酰胆碱受体(alpha 7 nicotinic acetylcholine receptor,α7-nAChR)联合钙离子(calcium ion,Ca^(2.)对LPS刺激的人牙髓干细胞(dental pulp stem cell,DPSC)牙/骨向分化的影响。方法:分离培养DPSC,流式细胞术对DPSC进行表面标志物表达鉴定。CCK-8检测α7-nAChR激动剂PNU-2.2.87和Ca^(2.)对DPSC增殖的影响。通过碱性磷酸酶(alkaline phosphatase,ALP)活性和染色筛选PNU-2.2.87促进DPSC表达ALP活性的最佳浓度。用大肠杆菌脂多糖(lipopolysaccharide,LPS)模拟炎性微环境刺激DPSC。采用免疫印迹分析(Western blot,WB)、实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,RT-qPCR)和茜素红染色等方法检测牙/骨向分化的相关蛋白:Ⅰ型胶原(typeⅠcollagen,COL-I)、牙本质涎磷蛋白(dentin sialoprotein,DSPP)、骨钙素(osteopontin,OPN)、ALP、核心转录因子-2.runt-related transcription factor 2.RUNX2.、成骨细胞特异性转录因子(osterix,OSX),相关基因(COL-I、DSPP、OPN、ALP、RUNX2.OSX)和矿化基质表达情况。Fura-2.M用于检测细胞内Ca^(2.)流动情况。结果:CCK-8实验显示,PNU-2.2.87浓度低于10μmol/L时对细胞增殖无抑制作用,且此浓度处理LPS刺激的DPSC后ALP活性增加最明显;Ca^(2.)浓度低于2.mmol/L对细胞增殖无抑制作用;Western blot和RT-qPCR实验显示,PNU-2.2.87及Ca^(2.)处理后的LPS刺激的DPSC牙/骨向分化相关蛋白(COL-I、DSPP、OPN、ALP、RUNX2.OSX)和相关基因(COL-I、DSPP、OPN、ALP、RUNX2.OSX)的表达及矿化基质形成均明显上调,二者联合后上调最显著(P 2.AM钙离子探针结果显示DPSC细胞内Ca^(2.)浓度增加。结论:10μmol/L PNU-2.2.87联合2.mmol/L Ca^(2.)可以促进LPS刺激的DPSC的牙/骨向分化能力。

关键词： α7乙酰胆碱受体牙/骨向分化人牙髓干细胞钙离子脂多糖

Efficient fabrication of light Cf/SiHfBOC composites with excellent thermal shock resistance and ultra-high-temperature ablation up to 1800℃

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Journal of Advanced Ceramics 2023年第11期12卷 2062-2074页

作者： Yang Lyu Zhihong Han guangdong Zhao Yuan Cheng Shanbao Zhou Xinghong Zhang Guiqing Chen Wenbo Han National Key Laboratory of Science and Technology on Advanced Composites in Special Environments and Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080China School of Chemistry and Materials Science Heilongjiang UniversityHarbin 150080China

In this paper,a high-yield Hf-modified SiHfBOC ceramic precursor was developed,and a high-pressure assisted impregnation pyrolysis method was proposed to achieve the preparation of 3D PyC–Cf/SiHfBOC *** high-pressure assisted impregnation method significantly improves impregnation filling effect of the precursor in and between fiber bundles compared to dozens of traditional impregnation *** undergoing just 9 precursor infiltration pyrolysis(PIP)cycles,the composites achieved relative density of approximately 90%and density of 1.64.g/cm^(3).The critical temperature difference of the 3D PyC–Cf/SiHfBOC composites after the shock of room temperature(RT)–1000℃is as high as 650℃,which is twice that of traditional ceramic materials,showing good thermal shock *** the effect of Hf modification,a dense HfO_(2.–SiO_(2.oxide layer(thickness of 93μm)was formed in situ on the surface of the 3D PyC–Cf/SiHfBOC composites,effectively preventing further erosion of the composite matrix by high-temperature oxidation *** in the ultra-high-temperature oxygen-containing environment at 1800℃,it still exhibits an excellent non-ablative result(with a linear ablation rate of 0.83×10^(−4.mm/s).This work not only enriches the basic research on lightweight ultra-high-temperature ceramic composites converted from Hf ceramic precursors,but also provides strong technical support for their applications in ultra-high-temperature non-ablative thermal protection materials for high-speed aircraft.

关键词： 3D PyC-C/SiHfBOC composites precursor infiltration pyrolysis(PIP)method thermal shock resistance ablation resistance ablation mechanism

Association mapping for bacterial wilt resistance in tobacco

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Association mapping for bacterial wilt resistance in tobacco

慢性乙型肝炎患者外周血T淋巴细胞亚群变化与病毒复制的关系

第十三届全国植物基因组学大会

作者： Yiwen Yu~(1,4.,Zijuan Zhang~(1,) Yanshi Xia~(1,4.,Ronghua Li~(1,4.) Miaowen Qiu~2.Junbiao Chen~3, Zhenchen Zhang~3,Peiguo Guo~(1,4.) 1 Internation center For Crop Stress Resistance/college of Life Sciences,Guangzhou University,Guangzhou 510006,China 2.Nanxiong Research Institute of guangdong Tobacco Co Ltd,Nanxiong 512.0}0,China 3 Crop Research Institute,guangdong Academy of Agricultural Sciences,Guangzhou 51064., China 4.Guangzhou Key Laboratory For Plant Stress resistant Functional Gene /college of Life Sciences,Guangzhou University,Guangzhou 510006,China

Bacterial wilt,caused by Ralstonia solanacearum,is found in almost all warm temperate and semitropical zones of the world,and is a serious disease of tobacco in the southern part of China,.Growing tobacco cultivar resistant to bacterial wilt is one of best measures to control the disease and reduce the loss,while molecular-

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检验医学 2009年第11期24卷 808-811页

作者：何英张允奇吴润香陆学东广东医学院附属深圳市福田人民医院广东深圳518033

目的探讨慢性乙型肝炎患者外周血T淋巴细胞亚群变化与乙型肝炎病毒(HBV)复制的关系。方法用流式细胞术对81例慢性乙型肝炎患者和30名健康对照者进行外周血T淋巴细胞亚群检测,用荧光定量聚合酶链反应(PCR)测定血清中的HBV DNA含量。结果... 详细信息

目的探讨慢性乙型肝炎患者外周血T淋巴细胞亚群变化与乙型肝炎病毒(HBV)复制的关系。方法用流式细胞术对81例慢性乙型肝炎患者和30名健康对照者进行外周血T淋巴细胞亚群检测,用荧光定量聚合酶链反应(PCR)测定血清中的HBV DNA含量。结果慢性乙型肝炎患者外周血CD3+、CD4.T细胞百分率及CD4./CD8+比值较健康对照者显著降低,而CD8+T细胞百分率显著升高。与健康对照组相比,HBV DNA阴性患者只有CD4./CD8+比值变化显著;HBV DNA阳性患者CD3+、CD4.、CD8+T细胞百分率及CD4./CD8+比值有显著改变,但高拷贝组和低拷贝组相比,差异无统计学意义。结论慢性乙型肝炎患者感染HBV后免疫功能下降与病毒复制有关,HBV复制可进一步加重免疫功能紊乱。

关键词： T淋巴细胞亚群流式细胞术聚合酶链反应慢性乙型肝炎

Pigment Epithelium-Derived Factor Enhances Tumor Response to Radiation by Normalizing Vasculature in Xenografted Model of Lung Cancer

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Pigment Epithelium-Derived Factor Enhances Tumor Response to...